Ratlarda Gentamisin İle İndüklenmiş Oksidatif Streste Borun Muhtemel Koruyucu Etkisinin Dokularda Araştırılması#

Year 2017, Volume: 10 Issue: 3, 172 - 179, 01.03.2017

İsmail Küçükkurt Damla Arslan Acaröz Hasan Hüseyin Demirel Sinan İnce Abdullah Eryavuz

Abstract

Çalışmada gentamisin maruziyeti ile toksisite oluşturulan ratlarda borun (B) muhtemel koruyucu etkisinin belirlenmesi amaçlandı. Bu amaçla Wistar albino 56 erkek rat kullanıldı. Her grupta 7 hayvan olacak şekilde, kontrol, gentamisin (100 mg/kg i.p.), B-5 (5 mg/kg B, i.p.), B-10 (10 mg/kg B, i.p.), B-20 (20 mg/kg B, i.p.), B-5 + gentamisin (5 mg/kg B ve 100 mg/kg gentamisin, i.p.), B-10 + gentamisin (10 mg/kg B ve 100 mg/kg gentamisin, i.p.), B-20 + gentamisin (20 mg/kg B ve 100 mg/kg gentamisin, i.p.) olarak 8 gruba ayrıldı. Ratlara B uygulaması gentamisin enjeksiyonundan 4 gün önce verilmeye başlandı. Gentamisin uygulamasına 4. gün başlandı ve 12. gün bu uygulama sonlandırıldı. Gentamisin uygulamasının bitişinden itibaren hayvanlara 2 gün daha B verildi. Uygulama sonunda hayvanlardan anestezi altında karaciğer, kalp, beyin, akciğer ve testis dokuları alındı. Gentamisinin karaciğer dokusunda malondialdehid düzeylerini arttırdığı, buna karşın redükte glutatyon düzeylerini, superoksid dismutaz ve katalaz aktivitelerini azalttığı tespit edildi. Histopatolojik incelemede gentamisinin dokularda hasar oluşturduğu gözlendi. Buna karşın, B uygulamasının gentamisin ile indüklenen hasarı azalttığı tespit edildi.

Keywords

Bor, Gentamisin, Histopatoloji, Lipid Peroksidasyon, Oksidatif Stres, Rat

Potential Protective Effect of Boron Against Gentamicin-Induced Oxidative Stress on Rat Tissues

Abstract

The objective of this study was to determine the protective effects of boron on gentamicin induced toxicity in rats. Rats were divided into eight experimental groups containing seven animals in each group. Experimental groups were as follows; control group (fed without B), gentamicin group (100 mg/kg, i.p.), B-5 group (5 mg/kg B, i.p.), B-10 group (10 mg/kg B, i.p.), B-20 group (20 mg/kg B, i.p.), B-5 + gentamicin group (5 mg/kg B and 100 mg/kg gentamicin, i.p.), B-10 + gentamicin group (10 mg/kg B and 100 mg/kg gentamicin, i.p.), and B-20 + gentamicin group (20 mg/kg B and 100 mg/kg gentamicin, i.p.). B was given to rats before four days. Gentamicin was given to rats on the fourth day and finished on the twelfth day. Also, administration of B was completed the fourteenth day. End of the experiment, liver, hearth, brain, lung, and testis tissues were collected from animals under anaesthesia. Administration of gentamicin increased malondialdehyde levels whereas decreased reduce glutathione levels, superoxide dismutase and catalase activities in the liver. Histopathologically, damages were detected in the tissues of gentamicin group. However, treatment of boron resulted in a reversal of gentamicin-induced damages.

Keywords

Boron, Gentamicin, Histopathology, Lipid Peroxidation, Oxidative Stress, Rat

References

• Aebi H. Catalase in vitro, in: U. Bergmeyer (Ed.), Methods of enzymatic analysis. Academic Press, New York and London. 1974; 673- 677.
• Atessahin A, Karahan I, Yilmaz S, Çeribaşi AO, Princci I. The effect of manganese chloride on gentamicin-induced nephrotoxicity in rats. Pharmacological Research. 2003; 48: 637-642.
• Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione. Journal of Laboratoty and Clinical Medicine. 1993; 61: 882-888.
• Bilgiç M, Dayık M. Borun özellikleri ve tekstil endüstrisinde kullanımıyla sağladığı avantajlar. Tekstil Teknolojileri Elektronik Dergisi. 2013; 7: 27-37.
• Bourgeois AC, Scott ME, Sabally K, Koski KG. Low dietary boron reduces parasite (nematoda) survival and alters cytokine profiles but the infection modifies liver minerals in mice. Journal of Nutrition. 2007; 137: 2080-2086.
• Briggs GG, Ambrose P, Nageotte MP. Gentamicin dosing in postpartum women with endometritis. American Journal of Obstetrics and Gynecology. 1989; 160: 309- 13.
• Cuzzocrea S, Mazzon E, Dugo L, Serraino I, Di Paola R, Britti D, De Sarro A, Pierpaoli S, Caputi A, Masini E, Salvemini DA. Role for superoxide in gentamicin-mediated nephropathy in rats. European Journal of Pharmacology. 2002; 450: 67-76.
• Dökmeci İ, Akçasu A, Banoğlu N, Berkarda Ş. Farmakoloji. İlaç Uygulamalarında Temel Kavramlar. Editör: Dökmeci İ. Nobel Tıp Kitabevleri. 1992; 705-785.
• Duff P, Jorgensen JH, Gibbs RS, Blanco JD, Alexander G, Castaneda YS. Serum gentamicin levels in patients with postcesarean endomyometritis. Obstetric Gynecology. 1983; 61: 723-727.
• Hancock RE, Raffle VJ, Nicas TI. Involvement of the outer membrane in gentamicin and streptomycin uptake and killing in Pseudomonas aeruginosa. Antimicrobial Agents Chemotheraphy. 1981; 19: 777-785.
• Ince S., Arslan-Acaroz D. An Update on Health Effects of Metalloid Trace Element: Boron. Aperito Journal of Drug Designing and Pharmacology. 2015; 2:1.
• Ince S, Keles H, Erdogan M, Hazman O, Kucukkurt I. Protective effect of boric acid against carbon tetrachloride–induced hepatotoxicity in mice. Drug and chemical toxicology. 2012; 35: 285-292.
• Ince S, Kucukkurt I, Cigerci IH, Fidan AF, Eryavuz A. The effects of dietary boric acid and borax supplementation on lipid peroxidation, antioxidant activity, and DNA damage in rats. Journal of Trace Element and Medicinal Biology. 2010; 24: 161-164.
• Ince S, Kucukkurt I, Demirel HH, Acaroz DA, Akbel E, Cigerci IH. Protective effects of boron on cyclophosphamide induced lipid peroxidation and genotoxicity in rats. Chemosphere. 2014; 108: 197-204.
• Karahan I, Atessahin A, Yılmaz S, Ceribası AO, Sakin F. Protective effect of lycopene on gentamicin-induced oxidative stress and nephrotoxicity in rats. Toxicology. 2005; 215: 198-204.
• Kayaalp SO. Rasyonel Tedavi Yönünden Tıbbi Farmakoloji (Cilt 3). Ankara: Feryal Matbaası. 1990.
• Khan MR, Badar I, Siddiquah A. Prevention of hepatorenal toxicity with Sonchus asper in gentamicin treated rats. BMC complementary and alternative medicine. 2011; 11(1): 113.
• Khan SA, Priyamvada S, Farooq N, Khan S, Khan MW, Yusufi AN. Protective effect of green tea extract on gentamicin-induced nephrotoxicity and oxidative damage in rat kidney. Pharmacological Research. 2009; 59: 254-262.
• Kucukkurt I, Akbel E, Karabag F, Ince S. The effects of dietary boron compounds in supplemented diet on hormonal activity and some biochemical parameters in rats. Toxicology and industrial health. 2015; 31: 255-260.
• Kucukkurt I, Ince S, Fidan AF, Ozdemir A. The effects of dietary supplementation of different amount of Yucca schidigera powder (Sarsaponin 30®) on blood and tissue antioxidant defense systems and lipid peroxidation in rats. Journal of Animal and Veterinary Advances. 2008; 7: 1413-1417.
• Lode H, Kemmerich B, Koeppe P. Comparative clinical pharmacology of gentamicin, sisomicin, and tobramycin. Antimicrobial Agents Chemotheraphy. 1975; 8: 396-401.
• Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. The Journal of Biological Chemistry. 1951; 193: 265-275.
• Luo QH, Chen ML, Sun FJ, Chen ZL, Li MY, Zeng W, Gong L, Cheng AC, Peng X, Fang J, Tang L, Geng Y. KIM-1 and NGAL as biomarkers of nephrotoxicity induced by gentamicin in rats. Moleculer Cell Biochemistry. 2014; 397: 53-60.
• McCoy H, Kenney MA, Montgomery C, Irwin A, Williams L, Orrell R. Relation of boron to the composition and mechanical properties of bone. Environmental Health Perspective. 1994; 102: 49-53.
• Nakajima T, Hishida A, Kato A. Mechanisms for protective effects of free radical scavengers on gentamicin-mediated nephropathy in rats. American Journal of Physiology. 1994; 266: 425-431.
• Nielsen FH, Hunt CD, Mullen LM, Hunt JR. Effect of dietary boron on mineral,estrogen, and testosterone metabolism in postmenopausal women. Faseb Journal. 1987; 1: 394-397.
• Noorani AA, Gupta K, Bhadada K, Kale MK. Protective effect of methanolic leaf extract of Caesalpinia bonduc (L.) on gentamicininduced hepatotoxicity and nephrotoxicity in rats. Iranian Journal of Pharmacology and Therapeutics. 2011; 10: 21-25.
• Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry. 1979; 95: 351-358.
• Quirós Y, Vicente-Vicente L, Morales AI, Lopez-Novoa JM, Lopez-Hernandez FJ. An integrative overview on the mechanisms underlying the renal tubular cytotoxicity of gentamicin. Toxicological Sciences. 2011; 119: 245–256.
• Samman S, Naghii MR, Lyons Wall PM, Verus AP. The nutritional and metabolic effects of boron in humans and animals. Biological Trace Element Research. 1998; 66: 227-235.
• Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clinical Chemistry. 1988; 34: 497-500.
• Şanlı Y, Kaya S. Veteriner Farmakoloji ve İlaçla Sağıtım Seçenekleri. Medisan Yayınevi, Ankara. 1994; 571-650.
• Şener S. Veteriner Klinik Farmakoloji ve Formüller. Pethask Veteriner Hekimliği Yayınları. 1990; 83-91.
• Turkez H, Geyikoğlu F, Tatar A, Keleş S, Özkanç A. Effects of some boron compounds on peripheral human blood. Zeitschrift für Naturforschung. 2007; 62: 889-896.