Listeria spp. doğada yaygın olarak bulunmakta ve halk sağlığı açısından önemli derecede risk oluşturabilmektedir. Bu bakteriler mastitisli hayvanlar, hijyenik olmayan sağım ekipmanları, transport veya depolama esnasında çiğ süte bulaşabilmektedir. Yapılan bu çalışmada Afyonkarahisar ilinden toplanan 100 manda ve 100 inek sütü olmak üzere toplam 200 çiğ süt numunesi materyal olarak kullanıldı. Toplanan süt numuneleri ISO 11290-1 yöntemi kullanılarak Listeria spp. varlığı yönünden analiz edildi. Analiz edilen 100 çiğ manda süt örneğinin 1’inde (%1) ve 100 çiğ inek süt örneğinin 4’ünde (%4) Listeria spp. klasik kültür metodu ile izole edildikten sonra Microbact 12L test kiti kullanılarak identifiye edildi. Ayrıca izole ve identifiye edilen Listeria türleri PCR ile onaylandı. Sonuç olarak manda sütünden izole edilen ve onaylanan Listeria türlerinin L. seeligeri (%1) olduğu, inek sütlerinden izole edilenlerin ise L. seeligeri (%3) ve L. ivanovii (%1) olduğu belirlendi. Yapılan çalışma sonucunda Afyonkarahisar ilinden toplanan çiğ manda ve inek sütlerinde L. monocytogenes saptanmazken, L. seeligeri ve L. ivanovii tespit edilmiş olup bu çalışma sonucunda Afyonkarahisar ilinden toplanan çiğ manda ve inek sütlerinde Listeria türlerinin yaygın olarak bulunmadığı belirlendi. Ancak Listeria türlerinin doğada yaygın olarak bulunması nedeniyle saha tarama çalışmalarının periyodik olarak yapılması, özellikle süt ve süt ürünleri işletmelerinde hijyen ve sanitasyon kurallarına uyulması, bu işletmelerde görev alan personelin bilgilendirilmesi önerilmektedir.
Abay S, Aydin F, Sumerkan AB. Molecular
typing of Listeria spp. isolated from
different sources. Ankara Üniv Vet Fak
Derg. 2012; 59: 183-190.
Adesiyun AA, Webb LA, Romain H, Kaminjolo
JS. Prevalence of Salmonella, Listeria
monocytogenes, Campylobacter spp., Yersinia
enterocolitica and Cryptosporidium spp. in bulk
milk, cows’ faeces and effluents of dairy
farms in Trinidad. Rev Elev Med Vet Pays
Trop. 1996; 49: 303-309.
Akça D, Şahin M. Kars yöresi sığırlarından alınan
süt ve vajinal sıvap örneklerinden Listeria
türlerinin araştırılması. Kafkas Üniv Vet
Fak Derg. 2011; 17(6): 987-993.
Amajoud N, Leclercq A, Soriano JM, Bracq-
Dieye H, El Maadoudi M, Senhaji NS,
Kounnoun A, Moura A, Lecuit A,
Abrini J. Prevalence of Listeria spp. and
characterization of Listeria monocytogenes
isolated from food products in Tetouan,
Morocco. Food Control. 2018; 84: 436-
Anonim (2001). Sekizinci beş yıllık kalkınma planı.
Hayvancılık Özel İhtisas Komisyonu
Raporu. DPT: 2574. ÖİK: 587.
Beale DJ, Morrison PD, Palombo EA.
Detection of Listeria in milk using nontargeted
metabolic profiling of Listeria
monocytogenes: a proof-of-concept
application. Food Control. 2014; 42: 343-
346.
Boland MJ, Rae AN, Vereijken JM, Meuwissen
MP, Fischer AR, van Boekel MA,
268
Rutherfurd SM, Gruppen H, Moughan
PJ, Hendriks WH. The future supply of
animal-derived protein for human
consumption. Trends Food Sci Technol.
2013; 29(1): 62-73.
Carpentier B, Cerf O. Review-Persistence of
Listeria monocytogenes in food industry
equipment and premises. Int J Food
Microbiol. 2011; 145(1): 1-8.
Çiftçioğlu G, Ülgen MT, Bostan K. Listeria
Monocytogenes’in Dondurmalardaki
varlığı üzerine bir araştırma. İstanbul Üniv
Vet Fak Derg. 1992; 18(2): 1-8.
Dhama K, Karthik K, Tiwari R, Shabbir MZ,
Barbuddhe S, Malik SVS, Singh RK.
Listeriosis in animals, its public health
significance (food-borne zoonosis) and
advances in diagnosis and control: a
comprehensive review. Veterinary
Quarterly. 2015; 35(4): 211-235.
Dümen E, Issa G, Ikiz S, Bagcıgil F, Ozgur Y,
Kahraman T, Ergin S, Yesil O.
Determining existence and antibiotic
susceptibility status of Listeria
monocytogenes isolated from dairy
products, serological and molecular typing
of the isolates. Kafkas Üniv Vet Fak Derg.
2011; 17(Suppl. A): 111-119.
Ertaş HB. Elazığ bölgesinde koyun ve keçi
sütlerinden Listeria türlerinin izolasyonu.
Doktora Tezi, Fırat Üniversitesi, Elazığ,
1997.
Gaya P, Sanchez J, Medina M, Nunez M.
Incidence of Listeria monocytogenes and
other Listeria species in raw milk produced
in Spain. Food Microbiol. 1998; 15: 551–
555.
Hasöksüz M, Ilgaz A. Marmara Bölgesindeki
sağlam koyunların kan serumlarında
ELISA yöntemi ile Listeria
monocytogenes’e karşı oluşan antikorların
saptanması ve listeriosis üzerinde
etiyolojik-epizootiolojik çalışmalar. İstanbul
Üniv Vet Fak Derg. 2000; 26: 157-174.
Hellberg RS, Martin KG, Keys AL, Haney CJ,
Shen Y, Smiley RD. 16S rRNA partial
gene sequencing for the differentiation and
molecular subtyping of Listeria species.
Food Microbiol. 2013; 36(2): 231e240.
Husu JR. Epidemiological studies on the
occurrence of Listeria monocytogenes in
the feces of dairy cattle. Zentralblatt fur
Veterinarmedizin. Reihe B. 1990; 37: 276-
282.
ISO-11290-1 (1996) Horizontal method for the
detection and enumeration of Listeria
monocytogenes, Part 2: Enumeration method,
International Organization for
Standardization, Geneva.
Lejeule JT, Rajala-Schultz P. Unpasteurized
milk: A continued public threat. Food
Safety. 2009; 48: 93-100.
Maertens de Noordhout C, Devleesschauwer
B, Angulo FJ, Verbeke G, Haagsma J,
Kirk M, Havelaar A, Speybroeck N. The
global burden of listeriosis: a systematic
review and meta-analysis. Lancet Infect
Dis. 2014; 14(11): 1073-1082.
Metin M. Süt teknolojisi, sütün bileşimi ve
işlenmesi. Ege Üniversitesi Mühendislik
Fakültesi Yayınları No: 33. Ege
Üniversitesi Basımevi, 802 s., Bornova,
İzmir, 2001.
Nightingale K. Listeria monocytogenes:
knowledge gained through DNA
sequence-based subtyping, implications,
and future considerations. Journal of
AOAC International. 2010; 93(4):
1275e1286.
Pereira PC. Milk nutritional composition and its
role in human health. Nutrition. 2014;
30(6), 619-627.
Rahimi E, Momtaz H, Behzadnia A,
Baghbadorani ZT. Incidence of Listeria
species in bovine, ovine, caprine, camel
and water buffalo milk using cultural
method and the PCR assay. Asian Pac J
Trop Dis. 2014; 4(1): 50-53.
Tekinşen OC. Süt Ürünleri Teknolojisi 3. Baskı
Selçuk Üniversitesi Basımevi, Konya, 2000.
Thorning TK., Raben A, Tholstrup T,
Soedamah-Muthu SS, Givens I, Astrup
A. Milk and dairy products: good or bad
for human health? An assessment of the
totality of scientific evidence. Food Nutr
Res. 2016; 60(1): 32527.
Uysal HK, Anğ Ö. Süt ve süt ürünlerinden izole
edilen Listeria türleri. Türk Mikrobiyoloji
Cemiyeti Dergisi. 2003; 33: 163-169.
Uyttendaele MR, Neyts KD, Lips RM,
Debevere JM. Indicence of Listeria
monocytogenes in poultry and poultry
products obtained from Belgian and
French abbatoirs. Food Microbiol. 1997;
14: 339-345.
Üçüncü M. A’dan Z’ye peynir teknolojisi. Cilt II.
Ege Üni. Mühendislik Fakültesi Gıda
Mühendisliği Bölümü, 2004; Sayfa 1233,
İzmir.
Ünlü GV, Ünlü M, Bakıcı MZ. Incidence of
Listeria spp. from raw milk in Sivas. Turk J
Med Sci. 1998; 28: 389-392.
Waak E, Tham W, Danielsson-Tham ML.
Prevalence and fingerprinting of Listeria
monocytogenes strains isolated from raw
whole milk in farm bulk tanks and dairy
plant receiving tanks. Appl Environ
Microbiol. 2002; 68: 3366-3370.
Determination of Listeria Species in Water Buffalo and Cow Milk Obtained From Afyonkarahisar Province
Year 2017,
Volume: 10 Issue: 4, 264 - 268, 01.12.2017
Listeria is one of the significant and commonly found bacteria in the environment which presents a high risk to human health. These bacteria can be transmitted to raw milk by mastitis infections, unhygienic milking equipment, transport, or storage. In this work, 200 raw milk samples (100 buffalo milk and 100 cow milk) collected from Afyonkarahisar province, were used as study material and milk samples were evaluated regarding the presence of Listeria spp. Detection of these bacteria was performed according to the method of ISO 11290-1. One Listeria spp. from 100 buffalo milk and four Listeria spp. from 100 cow milk samples were isolated by conventional method. The identification of isolated Listeria spp. was performed by Microbact 12L test kit. Also, isolated bacteria were confirmed by PCR technique. Isolated Listeria spp. from buffalo milk was determined as L. seeligeri (1%), those isolated from cow's milk were determined as L. seeligeri (3%) and L. ivanovii (%1). As a conclusion, this study shows that Listeria species are not widely present in raw buffalo milk and raw cow milk collected from Afyonkarahisar province. However, periodic field surveys are suggested due to the widespread presence of Listeria species in nature. Particularly, it is recommended to obey hygiene and sanitation rules and inform the staff working in milk and milk processing plant.
Abay S, Aydin F, Sumerkan AB. Molecular
typing of Listeria spp. isolated from
different sources. Ankara Üniv Vet Fak
Derg. 2012; 59: 183-190.
Adesiyun AA, Webb LA, Romain H, Kaminjolo
JS. Prevalence of Salmonella, Listeria
monocytogenes, Campylobacter spp., Yersinia
enterocolitica and Cryptosporidium spp. in bulk
milk, cows’ faeces and effluents of dairy
farms in Trinidad. Rev Elev Med Vet Pays
Trop. 1996; 49: 303-309.
Akça D, Şahin M. Kars yöresi sığırlarından alınan
süt ve vajinal sıvap örneklerinden Listeria
türlerinin araştırılması. Kafkas Üniv Vet
Fak Derg. 2011; 17(6): 987-993.
Amajoud N, Leclercq A, Soriano JM, Bracq-
Dieye H, El Maadoudi M, Senhaji NS,
Kounnoun A, Moura A, Lecuit A,
Abrini J. Prevalence of Listeria spp. and
characterization of Listeria monocytogenes
isolated from food products in Tetouan,
Morocco. Food Control. 2018; 84: 436-
Anonim (2001). Sekizinci beş yıllık kalkınma planı.
Hayvancılık Özel İhtisas Komisyonu
Raporu. DPT: 2574. ÖİK: 587.
Beale DJ, Morrison PD, Palombo EA.
Detection of Listeria in milk using nontargeted
metabolic profiling of Listeria
monocytogenes: a proof-of-concept
application. Food Control. 2014; 42: 343-
346.
Boland MJ, Rae AN, Vereijken JM, Meuwissen
MP, Fischer AR, van Boekel MA,
268
Rutherfurd SM, Gruppen H, Moughan
PJ, Hendriks WH. The future supply of
animal-derived protein for human
consumption. Trends Food Sci Technol.
2013; 29(1): 62-73.
Carpentier B, Cerf O. Review-Persistence of
Listeria monocytogenes in food industry
equipment and premises. Int J Food
Microbiol. 2011; 145(1): 1-8.
Çiftçioğlu G, Ülgen MT, Bostan K. Listeria
Monocytogenes’in Dondurmalardaki
varlığı üzerine bir araştırma. İstanbul Üniv
Vet Fak Derg. 1992; 18(2): 1-8.
Dhama K, Karthik K, Tiwari R, Shabbir MZ,
Barbuddhe S, Malik SVS, Singh RK.
Listeriosis in animals, its public health
significance (food-borne zoonosis) and
advances in diagnosis and control: a
comprehensive review. Veterinary
Quarterly. 2015; 35(4): 211-235.
Dümen E, Issa G, Ikiz S, Bagcıgil F, Ozgur Y,
Kahraman T, Ergin S, Yesil O.
Determining existence and antibiotic
susceptibility status of Listeria
monocytogenes isolated from dairy
products, serological and molecular typing
of the isolates. Kafkas Üniv Vet Fak Derg.
2011; 17(Suppl. A): 111-119.
Ertaş HB. Elazığ bölgesinde koyun ve keçi
sütlerinden Listeria türlerinin izolasyonu.
Doktora Tezi, Fırat Üniversitesi, Elazığ,
1997.
Gaya P, Sanchez J, Medina M, Nunez M.
Incidence of Listeria monocytogenes and
other Listeria species in raw milk produced
in Spain. Food Microbiol. 1998; 15: 551–
555.
Hasöksüz M, Ilgaz A. Marmara Bölgesindeki
sağlam koyunların kan serumlarında
ELISA yöntemi ile Listeria
monocytogenes’e karşı oluşan antikorların
saptanması ve listeriosis üzerinde
etiyolojik-epizootiolojik çalışmalar. İstanbul
Üniv Vet Fak Derg. 2000; 26: 157-174.
Hellberg RS, Martin KG, Keys AL, Haney CJ,
Shen Y, Smiley RD. 16S rRNA partial
gene sequencing for the differentiation and
molecular subtyping of Listeria species.
Food Microbiol. 2013; 36(2): 231e240.
Husu JR. Epidemiological studies on the
occurrence of Listeria monocytogenes in
the feces of dairy cattle. Zentralblatt fur
Veterinarmedizin. Reihe B. 1990; 37: 276-
282.
ISO-11290-1 (1996) Horizontal method for the
detection and enumeration of Listeria
monocytogenes, Part 2: Enumeration method,
International Organization for
Standardization, Geneva.
Lejeule JT, Rajala-Schultz P. Unpasteurized
milk: A continued public threat. Food
Safety. 2009; 48: 93-100.
Maertens de Noordhout C, Devleesschauwer
B, Angulo FJ, Verbeke G, Haagsma J,
Kirk M, Havelaar A, Speybroeck N. The
global burden of listeriosis: a systematic
review and meta-analysis. Lancet Infect
Dis. 2014; 14(11): 1073-1082.
Metin M. Süt teknolojisi, sütün bileşimi ve
işlenmesi. Ege Üniversitesi Mühendislik
Fakültesi Yayınları No: 33. Ege
Üniversitesi Basımevi, 802 s., Bornova,
İzmir, 2001.
Nightingale K. Listeria monocytogenes:
knowledge gained through DNA
sequence-based subtyping, implications,
and future considerations. Journal of
AOAC International. 2010; 93(4):
1275e1286.
Pereira PC. Milk nutritional composition and its
role in human health. Nutrition. 2014;
30(6), 619-627.
Rahimi E, Momtaz H, Behzadnia A,
Baghbadorani ZT. Incidence of Listeria
species in bovine, ovine, caprine, camel
and water buffalo milk using cultural
method and the PCR assay. Asian Pac J
Trop Dis. 2014; 4(1): 50-53.
Tekinşen OC. Süt Ürünleri Teknolojisi 3. Baskı
Selçuk Üniversitesi Basımevi, Konya, 2000.
Thorning TK., Raben A, Tholstrup T,
Soedamah-Muthu SS, Givens I, Astrup
A. Milk and dairy products: good or bad
for human health? An assessment of the
totality of scientific evidence. Food Nutr
Res. 2016; 60(1): 32527.
Uysal HK, Anğ Ö. Süt ve süt ürünlerinden izole
edilen Listeria türleri. Türk Mikrobiyoloji
Cemiyeti Dergisi. 2003; 33: 163-169.
Uyttendaele MR, Neyts KD, Lips RM,
Debevere JM. Indicence of Listeria
monocytogenes in poultry and poultry
products obtained from Belgian and
French abbatoirs. Food Microbiol. 1997;
14: 339-345.
Üçüncü M. A’dan Z’ye peynir teknolojisi. Cilt II.
Ege Üni. Mühendislik Fakültesi Gıda
Mühendisliği Bölümü, 2004; Sayfa 1233,
İzmir.
Ünlü GV, Ünlü M, Bakıcı MZ. Incidence of
Listeria spp. from raw milk in Sivas. Turk J
Med Sci. 1998; 28: 389-392.
Waak E, Tham W, Danielsson-Tham ML.
Prevalence and fingerprinting of Listeria
monocytogenes strains isolated from raw
whole milk in farm bulk tanks and dairy
plant receiving tanks. Appl Environ
Microbiol. 2002; 68: 3366-3370.
Acaröz, U., Acaröz-arslan, D., Kara, R., Zemheri, F., et al. (2017). Determination of Listeria Species in Water Buffalo and Cow Milk Obtained From Afyonkarahisar Province. Kocatepe Veterinary Journal, 10(4), 264-268.
AMA
Acaröz U, Acaröz-arslan D, Kara R, Zemheri F, Gürler Z. Determination of Listeria Species in Water Buffalo and Cow Milk Obtained From Afyonkarahisar Province. kvj. December 2017;10(4):264-268.
Chicago
Acaröz, Ulaş, Damla Acaröz-arslan, Recep Kara, Fahriye Zemheri, and Zeki Gürler. “Determination of Listeria Species in Water Buffalo and Cow Milk Obtained From Afyonkarahisar Province”. Kocatepe Veterinary Journal 10, no. 4 (December 2017): 264-68.
EndNote
Acaröz U, Acaröz-arslan D, Kara R, Zemheri F, Gürler Z (December 1, 2017) Determination of Listeria Species in Water Buffalo and Cow Milk Obtained From Afyonkarahisar Province. Kocatepe Veterinary Journal 10 4 264–268.
IEEE
U. Acaröz, D. Acaröz-arslan, R. Kara, F. Zemheri, and Z. Gürler, “Determination of Listeria Species in Water Buffalo and Cow Milk Obtained From Afyonkarahisar Province”, kvj, vol. 10, no. 4, pp. 264–268, 2017.
ISNAD
Acaröz, Ulaş et al. “Determination of Listeria Species in Water Buffalo and Cow Milk Obtained From Afyonkarahisar Province”. Kocatepe Veterinary Journal 10/4 (December 2017), 264-268.
JAMA
Acaröz U, Acaröz-arslan D, Kara R, Zemheri F, Gürler Z. Determination of Listeria Species in Water Buffalo and Cow Milk Obtained From Afyonkarahisar Province. kvj. 2017;10:264–268.
MLA
Acaröz, Ulaş et al. “Determination of Listeria Species in Water Buffalo and Cow Milk Obtained From Afyonkarahisar Province”. Kocatepe Veterinary Journal, vol. 10, no. 4, 2017, pp. 264-8.
Vancouver
Acaröz U, Acaröz-arslan D, Kara R, Zemheri F, Gürler Z. Determination of Listeria Species in Water Buffalo and Cow Milk Obtained From Afyonkarahisar Province. kvj. 2017;10(4):264-8.