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Koyunlarda Yeme Nane İlavesinin Rumenprotozoon Sayısı ve Bazı Rumen Parametreleri İle Kan Oksidan-Antioksidan Dengeye Etkisi

Year 2017, Volume 10, Issue 4, 300 - 307, 01.12.2017

Abstract

Bu çalışma, koyunlarda yeme nane ilavesinin rumen protozoon sayısı ile bazı rumen ve kan parametrelerine etkisini belirlemek amacıyla yapıldı. Çalışmada 18 adet koyun kullanılmış olup, bunlar her grupta 6 adet olacak şekilde 3 gruba ayrıldı. Toplam 30 gün süren deneme periyodu boyunca, Kontrol, %2.5 Nane ve %5 Nane gruplarındaki koyunlar sırasıyla nane içermeyen, %2.5 nane ve %5 nane ilave edilmiş yemlerle beslendi. Denemenin sonunda koyunlardan rumen ve kan örnekleri alındı. Rumen örneklerinde; protozoon sayısı, pH ve amonyak düzeyleri belirlendi. Kan örneklerinde ise kan sayımı, plazma üre, glikoz, malondialdehid (MDA) ve glutasyon (GSH) düzeyleri, eritrositlerde süperoksit dismutaz (SOD) ve katalaz (CAT) enzim aktiviteleri belirlendi. Çalışmada; gruplar arasında rumen protozoon sayısı, pH ve amonyak düzeyleri bakımından istatistiksel farklar olduğu tespit edildi. Yeme nane ilavesinin hematolojik parametreler ile plazma üre, glikoz, MDA ve GSH düzeyleri, SOD ve CAT enzim aktivitelerine etkisinin olmadığı bulundu. Bu çalışmada elde edilen bulgular, koyunların yemine %5 düzeyinde nane ilavesinin hematolojik parametreler ile kan oksidan-antioksidan dengeyi etkilemeksizin rumen protozoon sayısını azalttığını gösterdi.

References

  • Abdel-Wareth, A. A. ve Lohakare, J. D. 2014. Effect of dietary supplementation of peppermint on performance, egg quality, and serum metabolic profile of Hy-Line Brown hens during the late laying period. Anim. Feed Sci. Tech. 197: 114–120.
  • Agarwal, N., Shekhar, C., Kumar, R., Chaudhary, L. C., Kamra, D. N. 2009. Effect of peppermint (Mentha piperita) oil on in vitro methanogenesis and fermentation of feed with buffalo rumen liquor. Anim. Feed Sci. Technol., 148(2), 321-327.
  • Akdogan, M., Gultekin, F., Yontem, M., 2004b. Effect of Mentha piperita (Labiatae) and Mentha spicata (Labiatae) on iron absorption in rats. Toxicol. Ind. Health., 20; 119-122.
  • Akdogan, M., Ozguner, M., Kocak, A., Oncu, M., Cicek, E., 2004a.Effects of peppermint teas on plasma testosterone, follicle-stimulating hormone, and luteinizing hormone levels and testicular tissue in rats. Urology, 64: 394-398.
  • Ando S. N., İshida M., Hosoda K., Bayaru E. (2003). Effect of peppermint feeding on the digestibility, ruminal fermentation and protozoa. Livestock Production Sci., 82: 245–248.
  • Benchaar C., Calsamiglia S., Chaves AV., Fraser GR., Colombatto D., McAllister TA., Beauchemin KA. 2008. A review of plant-derived essantial oils in ruminant nutrition and production. Anim.Feed Sci. Technol., 145; 209-228.
  • Beutler, E., Olga, D., Barbara, M.R. 1963. Improved method for the determinetion of blood glutathione. J. Lab.Clin.Med., 61; 882-888.
  • Bölükbaşı, F. 1989. Fizyoloji Ders Kitabı (Vücut Isısı ve Sindirim), Cilt I., A.Ü. Vet. Fak. Yayınları, AÜ Basımevi, Ankara.
  • Çolpan, İ. ve ark. (2014). Hayvan besleme ve beslenme hastalıkları. Ankara
  • Devi K.M, Palod J, Singh D.V., Singh C.B., Dar A.H., Singh P.K., Singh V. S. 2017.Effect of Pudina (Mentha arvensis) Leaf Powder on Biochemical Parameters in White Leghorn.Int.J.Live.Res., 7; 206-216.
  • Djouvinov, D., Pavlov, D., Ilchev, A., Enev, E., 1997. Peppermint (Mentha piperita huds.) and basil (Ocimum basilicum L.) etheric oil by-products as roughages for sheep feeding. Anim. Feed Sci. Technol., 68; 287- 294.
  • Draper, H. H., Hardley, M. 1990. Malondialdehyde determination as index of lipid peroxidation. Methods Enzymol. 186: 421-431.
  • Eryavuz A., 2000. Defaunasyonun ruminantların sindirimine etkileri (Derleme). Hayv. Araş. Derg., 10 (1-2); 78-84.
  • Eryavuz, A., 2004. Saponinler ve ruminantlarda rumen protozoon sayısının azaltılmasında bunların kullanılması (Derleme). Hayv. Araş.Derg., 13, (1-2); 60-66.
  • Eryavuz A. Dehority BA. 2009. Effects of supplemental zinc concentration on cellulose digestion and cellulolytic and total bacterial numbers in vitro. Anim. Feed Sci. Technol., 151; 175-183.
  • Eryavuz, A., Dundar, Y., Özdemir, M., Aslan, R., Tekerli, M., 2003. Effects of adding urea and sulfur on performance of faunate and defaunate Ramlic lambs, and some rumen and blood parameters. Anim.Feed Sci.Technol.109; 35-46.
  • Eryavuz A., Küçükkurt İ., İnce S., Fidan AF., Avcı G., Bülbül T. 2015. Kuzularda rasyona Yucca schidigera tozu katılması ve günlük dozunun rumen fermentasyonu ile verime etkilerinin araştırılması. Kocatepe Vet. J., 8; 1-10.
  • Hosoda, K., Kuramoto, K., Eruden, B., Nishida, T., & Shioya, S. 2006. The effects of three herbs as feed supplements on blood metabolites, hormones, antioxidant activity, IgG concentration, and ruminal fermentation in Holstein steers. Asian Australasian Journal of Animal Sciences, 19(1), 35.
  • İnce S., Küçükkurt I., Cigerci İH., Fidan AF., Eryavuz A. 2010. The effects of dietary boric acid and borax supplementation on lipid peroxidation, antioxidant activity, and DNA damage in rats. J. Tarce Elem. Med.Biol., 24; 161-164.
  • Jouany, J.P. and Morgavi, D.R. 2007. Use of natural products as alternatives to antibiotic feed additives in ruminant production. ANIMAL, 1; 1443-1466.
  • Khamısabadı, H., Kafılzadeh, F., Charaıen, B. 2016. Effect of thyme (Thymus vulgaris) or peppermint (Mentha piperita) on performance, digestibility and blood metabolites of fattening Sanjabi lambs. Biharean Biologist, 10(2).
  • Khorrami B, Vakili AR, Danesh Mesgaran M, Klevenhusen F. Thyme and cinnamon essential oils: Potential alternatives for monensin as a rumen modifier in beef production systems. Anim Feed Sci Technol. 2015. 200; 8-16.
  • Luck H (1955) Catalase. in: Bergmeyer, H.U. (Ed.), Methods in Analysis. Academy Press, London.
  • Mahgoub, O., Kadim, I.T., Tageldin, M.H., Al-Marzooqi, W.S., Khalaf, S.Q., Ambu Ali, A. 2008. Clinical profile of sheep fed non-conventional feeds containing phenols and condensed tannins. Small Rum. Res. 78: 115–122.
  • Raghuvansi, S. K. S., Prasad, R., Mishra, A. S., Chaturvedi, O. H., Tripathi, M. K., Misra, A. K., Jakhmola, R. C. 2007. Effect of inclusion of tree leaves in feed on nutrient utilization and rumen fermentation in sheep. Bioresource technology, 98(3), 511-517.
  • Rochfort S., Parker AJ., Dunshea FR. 2008. Plant bioactives for ruminant health and productivity. Phytochemistry, 69; 299– 322.
  • Roshan N., Riley TV., Hammer KA. 2017.Antimicrobial activity of natural products against Clostridium difficile in vitro. J Appl.Microbiol., 123; 92-103.
  • Sulu, N., Bölükbaşı, F., Börkü, K. 1988. “Merinos koyunları rumen sıvısında protozoa sayısı ve bazı protozoon tiplerinin identifikasyonu”, A.Ü.Vet.Fak.Derg., 35 (1); 157-168. Sun Y, Oberley LW, Li Y (1988) A simple method for clinical assay of superoxide dismutase. Clin Chem 34:497-500.
  • Taghavi-Nezhad M., Alipour D., Flythe MD., 2014. The effect of essential oils of Zataria multiflora and Mentha spicata on the in vitro rumen fermentation, and growth and deaminative activity of amino acidfermenting bacteria isolated from Mebraban sheep. Anim Prod.Sci., 3; 299- 307.
  • Wallace RJ., McEwan NR., McIntosh FM., Teferedegne B., Newbold CJ. 2002. Natural Products as Manipulators of Rumen Fermentation. Asian-Aust. J. Anim. Sci.,15: 1458-1468.
  • Wanapat, M., Kang, S., Khejornsart, P., Wanapat, S. 2013. Effects of plant herb combination supplementation on rumen fermentation and nutrient digestibility in beef cattle. Asian-Aust. J Anim.Sci., 26;1127-1136.
  • Witterbourn CC, Hawkins RE, Brain M, Carrel W. The estimation of red cell superoxide dismutase activity. J Lab Clin Med. 1975. 55:337–41.
  • Yılmaz B. 2000. ‘Kan’ Fizyoloji, 2.Baskı, Feryal Matbaacılık, Ankara, S: 45-133.
  • Zmora, P., Cieslak, A., Pers-Kamczyc, E., Nowak, A., Szczechowiak, J., Szumacher-Strabel, M. 2012. Effect of Mentha piperita L. on in vitro rumen methanogenesis and fermentation. Acta Agriculturae Scandinavica, Section A– Animal Science, 62(1), 46-52.

Effect of Peppermint Supplementation to Diet on Rumen Protozoal Numbers and Some Ruminal Parameters in Sheep

Year 2017, Volume 10, Issue 4, 300 - 307, 01.12.2017

Abstract

This study was carried out to investigate the effects of dietary supplementation of peppermint on rumen protozoal numbers and some ruminal and hematological parameters in sheep. Eighteen sheep in a completely randomized desing were allocated to three treatments contained 6 sheep per group. During the experimental period (30 days), the sheep were fed to diets containing no peppermint, 2.5 % peppermint and 5% peppermint for groups of Control, 2.5%Peppermint and 5%Peppermint respectively. The rumen protozoal numbers, ruminal pH, rumen ammonia concentration, hematological parameters, plasma urea and glucose, malondialdehyde (MDA) and glutathione (GSH) concentrations, enzyme activities of superoxide dysmutase (SOD) and catalase (CAT) in ruminal and blood samples taken from sheep at the end of experiment were determined. Statistical significant differences were found in protozoal numbers, ruminal pH and rumen ammonia concentrations between three groups. Hematological parameters, concentrations of plasma urea, glucose, MDA and GSH, erythrocyte enzyme activities of SOD and CAT were not influenced by addition of peppermint to the diet. The results showed that addition of peppermint at a concentration of 5% to diet of sheep decreases ruminal protozoal numbers without affected hematological parameters and oxident-antioxident balance in the blood.

References

  • Abdel-Wareth, A. A. ve Lohakare, J. D. 2014. Effect of dietary supplementation of peppermint on performance, egg quality, and serum metabolic profile of Hy-Line Brown hens during the late laying period. Anim. Feed Sci. Tech. 197: 114–120.
  • Agarwal, N., Shekhar, C., Kumar, R., Chaudhary, L. C., Kamra, D. N. 2009. Effect of peppermint (Mentha piperita) oil on in vitro methanogenesis and fermentation of feed with buffalo rumen liquor. Anim. Feed Sci. Technol., 148(2), 321-327.
  • Akdogan, M., Gultekin, F., Yontem, M., 2004b. Effect of Mentha piperita (Labiatae) and Mentha spicata (Labiatae) on iron absorption in rats. Toxicol. Ind. Health., 20; 119-122.
  • Akdogan, M., Ozguner, M., Kocak, A., Oncu, M., Cicek, E., 2004a.Effects of peppermint teas on plasma testosterone, follicle-stimulating hormone, and luteinizing hormone levels and testicular tissue in rats. Urology, 64: 394-398.
  • Ando S. N., İshida M., Hosoda K., Bayaru E. (2003). Effect of peppermint feeding on the digestibility, ruminal fermentation and protozoa. Livestock Production Sci., 82: 245–248.
  • Benchaar C., Calsamiglia S., Chaves AV., Fraser GR., Colombatto D., McAllister TA., Beauchemin KA. 2008. A review of plant-derived essantial oils in ruminant nutrition and production. Anim.Feed Sci. Technol., 145; 209-228.
  • Beutler, E., Olga, D., Barbara, M.R. 1963. Improved method for the determinetion of blood glutathione. J. Lab.Clin.Med., 61; 882-888.
  • Bölükbaşı, F. 1989. Fizyoloji Ders Kitabı (Vücut Isısı ve Sindirim), Cilt I., A.Ü. Vet. Fak. Yayınları, AÜ Basımevi, Ankara.
  • Çolpan, İ. ve ark. (2014). Hayvan besleme ve beslenme hastalıkları. Ankara
  • Devi K.M, Palod J, Singh D.V., Singh C.B., Dar A.H., Singh P.K., Singh V. S. 2017.Effect of Pudina (Mentha arvensis) Leaf Powder on Biochemical Parameters in White Leghorn.Int.J.Live.Res., 7; 206-216.
  • Djouvinov, D., Pavlov, D., Ilchev, A., Enev, E., 1997. Peppermint (Mentha piperita huds.) and basil (Ocimum basilicum L.) etheric oil by-products as roughages for sheep feeding. Anim. Feed Sci. Technol., 68; 287- 294.
  • Draper, H. H., Hardley, M. 1990. Malondialdehyde determination as index of lipid peroxidation. Methods Enzymol. 186: 421-431.
  • Eryavuz A., 2000. Defaunasyonun ruminantların sindirimine etkileri (Derleme). Hayv. Araş. Derg., 10 (1-2); 78-84.
  • Eryavuz, A., 2004. Saponinler ve ruminantlarda rumen protozoon sayısının azaltılmasında bunların kullanılması (Derleme). Hayv. Araş.Derg., 13, (1-2); 60-66.
  • Eryavuz A. Dehority BA. 2009. Effects of supplemental zinc concentration on cellulose digestion and cellulolytic and total bacterial numbers in vitro. Anim. Feed Sci. Technol., 151; 175-183.
  • Eryavuz, A., Dundar, Y., Özdemir, M., Aslan, R., Tekerli, M., 2003. Effects of adding urea and sulfur on performance of faunate and defaunate Ramlic lambs, and some rumen and blood parameters. Anim.Feed Sci.Technol.109; 35-46.
  • Eryavuz A., Küçükkurt İ., İnce S., Fidan AF., Avcı G., Bülbül T. 2015. Kuzularda rasyona Yucca schidigera tozu katılması ve günlük dozunun rumen fermentasyonu ile verime etkilerinin araştırılması. Kocatepe Vet. J., 8; 1-10.
  • Hosoda, K., Kuramoto, K., Eruden, B., Nishida, T., & Shioya, S. 2006. The effects of three herbs as feed supplements on blood metabolites, hormones, antioxidant activity, IgG concentration, and ruminal fermentation in Holstein steers. Asian Australasian Journal of Animal Sciences, 19(1), 35.
  • İnce S., Küçükkurt I., Cigerci İH., Fidan AF., Eryavuz A. 2010. The effects of dietary boric acid and borax supplementation on lipid peroxidation, antioxidant activity, and DNA damage in rats. J. Tarce Elem. Med.Biol., 24; 161-164.
  • Jouany, J.P. and Morgavi, D.R. 2007. Use of natural products as alternatives to antibiotic feed additives in ruminant production. ANIMAL, 1; 1443-1466.
  • Khamısabadı, H., Kafılzadeh, F., Charaıen, B. 2016. Effect of thyme (Thymus vulgaris) or peppermint (Mentha piperita) on performance, digestibility and blood metabolites of fattening Sanjabi lambs. Biharean Biologist, 10(2).
  • Khorrami B, Vakili AR, Danesh Mesgaran M, Klevenhusen F. Thyme and cinnamon essential oils: Potential alternatives for monensin as a rumen modifier in beef production systems. Anim Feed Sci Technol. 2015. 200; 8-16.
  • Luck H (1955) Catalase. in: Bergmeyer, H.U. (Ed.), Methods in Analysis. Academy Press, London.
  • Mahgoub, O., Kadim, I.T., Tageldin, M.H., Al-Marzooqi, W.S., Khalaf, S.Q., Ambu Ali, A. 2008. Clinical profile of sheep fed non-conventional feeds containing phenols and condensed tannins. Small Rum. Res. 78: 115–122.
  • Raghuvansi, S. K. S., Prasad, R., Mishra, A. S., Chaturvedi, O. H., Tripathi, M. K., Misra, A. K., Jakhmola, R. C. 2007. Effect of inclusion of tree leaves in feed on nutrient utilization and rumen fermentation in sheep. Bioresource technology, 98(3), 511-517.
  • Rochfort S., Parker AJ., Dunshea FR. 2008. Plant bioactives for ruminant health and productivity. Phytochemistry, 69; 299– 322.
  • Roshan N., Riley TV., Hammer KA. 2017.Antimicrobial activity of natural products against Clostridium difficile in vitro. J Appl.Microbiol., 123; 92-103.
  • Sulu, N., Bölükbaşı, F., Börkü, K. 1988. “Merinos koyunları rumen sıvısında protozoa sayısı ve bazı protozoon tiplerinin identifikasyonu”, A.Ü.Vet.Fak.Derg., 35 (1); 157-168. Sun Y, Oberley LW, Li Y (1988) A simple method for clinical assay of superoxide dismutase. Clin Chem 34:497-500.
  • Taghavi-Nezhad M., Alipour D., Flythe MD., 2014. The effect of essential oils of Zataria multiflora and Mentha spicata on the in vitro rumen fermentation, and growth and deaminative activity of amino acidfermenting bacteria isolated from Mebraban sheep. Anim Prod.Sci., 3; 299- 307.
  • Wallace RJ., McEwan NR., McIntosh FM., Teferedegne B., Newbold CJ. 2002. Natural Products as Manipulators of Rumen Fermentation. Asian-Aust. J. Anim. Sci.,15: 1458-1468.
  • Wanapat, M., Kang, S., Khejornsart, P., Wanapat, S. 2013. Effects of plant herb combination supplementation on rumen fermentation and nutrient digestibility in beef cattle. Asian-Aust. J Anim.Sci., 26;1127-1136.
  • Witterbourn CC, Hawkins RE, Brain M, Carrel W. The estimation of red cell superoxide dismutase activity. J Lab Clin Med. 1975. 55:337–41.
  • Yılmaz B. 2000. ‘Kan’ Fizyoloji, 2.Baskı, Feryal Matbaacılık, Ankara, S: 45-133.
  • Zmora, P., Cieslak, A., Pers-Kamczyc, E., Nowak, A., Szczechowiak, J., Szumacher-Strabel, M. 2012. Effect of Mentha piperita L. on in vitro rumen methanogenesis and fermentation. Acta Agriculturae Scandinavica, Section A– Animal Science, 62(1), 46-52.

Details

Journal Section RESEARCH ARTICLE
Authors

İbrahim DURMUŞ This is me


Elmas ULUTAŞ This is me


Damla ACARÖZ-ARSLAN This is me


Sinan İNCE
0000-0002-1915-9797


İsmail KÜÇÜKKURT


Mehmet Naci SALİM This is me


Abdullah ERYAVUZ (Primary Author)
0000-0001-8602-2400

Publication Date December 1, 2017
Published in Issue Year 2017, Volume 10, Issue 4

Cite

Bibtex @research article { kvj367302, journal = {Kocatepe Veterinary Journal}, issn = {1308-1594}, eissn = {2147-6853}, address = {}, publisher = {Afyon Kocatepe University}, year = {2017}, volume = {10}, pages = {300 - 307}, doi = {}, title = {Effect of Peppermint Supplementation to Diet on Rumen Protozoal Numbers and Some Ruminal Parameters in Sheep}, key = {cite}, author = {Durmuş, İbrahim and Ulutaş, Elmas and Acaröz-arslan, Damla and İnce, Sinan and Küçükkurt, İsmail and Salim, Mehmet Naci and Eryavuz, Abdullah} }
APA Durmuş, İ. , Ulutaş, E. , Acaröz-arslan, D. , İnce, S. , Küçükkurt, İ. , Salim, M. N. & Eryavuz, A. (2017). Effect of Peppermint Supplementation to Diet on Rumen Protozoal Numbers and Some Ruminal Parameters in Sheep . Kocatepe Veterinary Journal , 10 (4) , 300-307 . Retrieved from https://dergipark.org.tr/en/pub/kvj/issue/32999/367302
MLA Durmuş, İ. , Ulutaş, E. , Acaröz-arslan, D. , İnce, S. , Küçükkurt, İ. , Salim, M. N. , Eryavuz, A. "Effect of Peppermint Supplementation to Diet on Rumen Protozoal Numbers and Some Ruminal Parameters in Sheep" . Kocatepe Veterinary Journal 10 (2017 ): 300-307 <https://dergipark.org.tr/en/pub/kvj/issue/32999/367302>
Chicago Durmuş, İ. , Ulutaş, E. , Acaröz-arslan, D. , İnce, S. , Küçükkurt, İ. , Salim, M. N. , Eryavuz, A. "Effect of Peppermint Supplementation to Diet on Rumen Protozoal Numbers and Some Ruminal Parameters in Sheep". Kocatepe Veterinary Journal 10 (2017 ): 300-307
RIS TY - JOUR T1 - Effect of Peppermint Supplementation to Diet on Rumen Protozoal Numbers and Some Ruminal Parameters in Sheep AU - İbrahim Durmuş , Elmas Ulutaş , Damla Acaröz-arslan , Sinan İnce , İsmail Küçükkurt , Mehmet Naci Salim , Abdullah Eryavuz Y1 - 2017 PY - 2017 N1 - DO - T2 - Kocatepe Veterinary Journal JF - Journal JO - JOR SP - 300 EP - 307 VL - 10 IS - 4 SN - 1308-1594-2147-6853 M3 - UR - Y2 - 2017 ER -
EndNote %0 Kocatepe Veterinary Journal Effect of Peppermint Supplementation to Diet on Rumen Protozoal Numbers and Some Ruminal Parameters in Sheep %A İbrahim Durmuş , Elmas Ulutaş , Damla Acaröz-arslan , Sinan İnce , İsmail Küçükkurt , Mehmet Naci Salim , Abdullah Eryavuz %T Effect of Peppermint Supplementation to Diet on Rumen Protozoal Numbers and Some Ruminal Parameters in Sheep %D 2017 %J Kocatepe Veterinary Journal %P 1308-1594-2147-6853 %V 10 %N 4 %R %U
ISNAD Durmuş, İbrahim , Ulutaş, Elmas , Acaröz-arslan, Damla , İnce, Sinan , Küçükkurt, İsmail , Salim, Mehmet Naci , Eryavuz, Abdullah . "Effect of Peppermint Supplementation to Diet on Rumen Protozoal Numbers and Some Ruminal Parameters in Sheep". Kocatepe Veterinary Journal 10 / 4 (December 2017): 300-307 .
AMA Durmuş İ. , Ulutaş E. , Acaröz-arslan D. , İnce S. , Küçükkurt İ. , Salim M. N. , Eryavuz A. Effect of Peppermint Supplementation to Diet on Rumen Protozoal Numbers and Some Ruminal Parameters in Sheep. Kocatepe Veterinary Journal. 2017; 10(4): 300-307.
Vancouver Durmuş İ. , Ulutaş E. , Acaröz-arslan D. , İnce S. , Küçükkurt İ. , Salim M. N. , Eryavuz A. Effect of Peppermint Supplementation to Diet on Rumen Protozoal Numbers and Some Ruminal Parameters in Sheep. Kocatepe Veterinary Journal. 2017; 10(4): 300-307.
IEEE İ. Durmuş , E. Ulutaş , D. Acaröz-arslan , S. İnce , İ. Küçükkurt , M. N. Salim and A. Eryavuz , "Effect of Peppermint Supplementation to Diet on Rumen Protozoal Numbers and Some Ruminal Parameters in Sheep", Kocatepe Veterinary Journal, vol. 10, no. 4, pp. 300-307, Dec. 2017

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