The Role of Pestiviruses (BDV and BVDV) in Ruminant Abortion Cases in the Afyonkarahisar Province

Year 2018, Volume: 11 Issue: 3, 238 - 244, 01.09.2018

Murat Şevik

https://doi.org/10.30607/kvj.418451

Cited By: 2

Abstract

Pestiviruses are important viral agents that can cause
abortion in ruminants. In this study, roles of Border Disease Virus (BDV) and
Bovine Viral Diarrhoea Virus (BVDV) were investigated in ruminant abortion
cases. Aborted foetal tissue samples were collected from 101 animals (74 sheep
foetuses and 27 bovine foetuses), each from epidemiologically different farms,
during the months of January 2016 and December 2017 in the Afyonkarahisar
Province. One step real-time duplex RT-PCR was used for the detection of BDV
and BVDV RNA. Genetic characterization of the field isolates of pestiviruses
was conducted by sequencing 5' untranslated region (5' UTR). BDV RNA was
detected in 9 (12.16%) of the 74 aborted sheep foetuses, whereas BVDV RNA was
detected in 6 (22.2%) of the 27 bovine foetuses. Phylogenetic analysis based on
the 5' UTR region indicated that BDV isolates in the present study belong to
BDV-7 genotype whereas BVDV isolates belong to BVDV-1 genotype. The results of
this study showed that pestivirus infections play important role in ruminant
abortion cases in Afyonkarahisar province.

Keywords

Border disease virus, bovine viral diarrhoea virus, abortion, sheep, cattle

Afyonkarahisar İlinde Ruminant Abort Vakalarında Pestivirusların (BDV ve BVDV) Rolleri

Abstract

Pestiviruslar
ruminantlarda abortlara neden olan önemli viral ajanlardır. Bu çalışmada,
ruminant abort vakalarında Border Disease Virus (BDV) ve Bovine Viral Diarrhoea
Virus (BVDV)’un rolleri araştırılmıştır. Abort olmuş fötus doku örnekleri 101
hayvandan (74’ü koyun fötusu, 27’si sığır fötus), her biri epidemiyolojik olarak
farklı çiftliklerden, Ocak 2016 ve Aralık 2017 ayları arasında Afyonkarahisar
ilinden toplanmıştır. BDV ve BVDV RNA’sının tespiti için tek adımlı real-time
dubleks RT-PCR yöntemi kullanılmıştır. Sahadan izole edilen pestivirus’ların
genetik karakterizasyonu 5' translate olmayan bölge sonunun (5' UTR) sekansı
ile gerçekleştirilmiştir. BDV RNA’sı, 74 aborte koyun fötusunun 9
(%12.16)’unda, BVDV RNA’sı ise 27 sığır fötusunun 6 (%22.2)’sında tespit
edilmiştir.  5' UTR bölgesinin
filogenetik analizi bu çalışmada izole edilen BDV izolatlarının BDV-7
genotipine, BVDV izolatlarının ise BVDV-1 genotipine ait olduğunu göstermiştir.
Bu çalışmanın sonuçları, pestivirus enfeksiyonlarının, Afyonkarahisar ilindeki
ruminant abort vakalarında önemli rol oynadığını göstermektedir.

Keywords

Border disease virus, bovine viral diarrhoea virus, koyun, abort, sığır

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