Pırlak Koyunlarında BMPR1B, BMP15 ve GDF9 Genlerinde Olası Polimorfizmlerin Araştırılması

Year 2018, Volume 11, Issue 4, 356 - 362, 15.12.2018

Koray ÇELİKELOĞLU Metin ERDOĞAN Özlem HACAN Serdar KOÇAK Zehra BOZKURT Mustafa TEKERLİ

https://doi.org/10.30607/kvj.428999

Abstract

Bu araştırmada, Afyonkarahisar İli Damızlık Koyun-Keçi Yetiştiricileri Birliği damızlık istasyonunda 2016 ve 2017 yıllarında çoklu ve tekli doğum yapanlardan sekizer baş olmak üzere toplam 16 baş Pırlak koyunda Bone Morphogenetic Protein Receptor type 1B (BMPR1B), Bone Morphogenetic Protein 15 (BMP15) ve Growth Differentiation Factor 9 (GDF9) genlerindeki polimorfizmin varlığı ve bunun tespit edilmesi durumunda çoklu doğumla ilişkilendirilmesi amaçlanmıştır. Genetik çalışmalarda kullanılmak üzere toplanan kan örnekleri hayvanların V. jugularis’lerinden EDTA’lı vakumlu tüplere alınmış ve genomik DNA izolasyon kiti kullanılarak DNA izolasyonları yapılmıştır. İlgili genler yönünden gerçekleştirilen PCR ve DNA dizileme analizleri sonucunda her üç gen yönünden de Pırlakların monomorfik yapıda olduğu ortaya konulmuştur.

Keywords

Pırlak, Döl verimi, BMPR 1B, BMP 15, GDF9

Investigation of Possible Polymorphisms in BMPR1B, BMP15 and GDF9 Genes in Pırlak Sheep

Abstract

This study was realised to determine the presence of polymorphism in major proliferacy genes named Bone Morphogenetic Protein Receptor type 1B (BMPR1B), Bone Morphogenetic Protein 15 (BMP15) and Growth Differentiation Factor 9 (GDF9) of 8 uniparous and 8 multiparous Pırlak sheep reared at Sheep and Goats Breeders Association’s Breeding Station, Afyonkarahisar between 2016 and 2017. Blood samples collected for using in genetic studies were taken from V. jugularis of animals into EDTA vacuum tubes and DNA isolations were performed using genomic DNA isolation kit. The PCR and DNA sequencing analyzes carried out on the basis of the respective genes revealed that Pırlaks are monomorphic in the aspect of above mentioned genes.

Keywords

Pırlak, Proliferacy, BMPR 1B, BMP 15, GDF9

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