Holştayn Sığırlarında Foliküler ve Luteal Fazdaki Ovaryum Dokularında İntraovarian Genlerin Ekspresyon Profili

Year 2018, Volume: 11 Issue: 4, 453 - 462, 15.12.2018

Selçuk Özdemir , Selim Çomaklı , Harun Arslan

https://doi.org/10.30607/kvj.430566

Abstract

Bu çalışmanın amacı,
Holştayn sığırlarına ait preovülatör folikül ve korpus luteum dokularında BMP15, TGFB1, TGFB2 ve GDF9 genlerinin ekspresyon seviyelerini
karşılaştırılmalı olarak belirlemektir. Bu amaç için, öncelikle dokular
immunohistokimyasal boyama ile incelendi. Daha sonra foliküler sıvılar ELISA
testiyle incelenerek östradiol ve progesteron seviyeleri belirlendi. Son olarak
qRT-PCR ile gruplar arasında ilgili genlere ait ekspresyon seviyeleri tespit
edildi. İmmunohistokimyasal boyama sonucunda preovülatör foliküllerde yoğun miktarda
östrojen reseptör alfa ve progesteron reseptör immunpozitifliklerine, korpus
luteum da çok hafif düzeyde östrogen alfa reseptör immunpozitifliğine,
progesteron reseptörlerinin ise preovülatör foliküllerdeki pozitiflik düzeyine
yakın olduğu belirlendi. Östradiol seviyesi, preovulatör foliküllerde yüksek,
progesteron seviyesi ise korpus luteumda yüksek olarak bulundu. Preovülatör
foliküllerdeki TGFB1 ve TGFB2 genlerine ait mRNA transkript
seviyesi korpus luteuma göre istatistiksel olarak daha yüksek bulundu (p<0.01, p<0.05, sırasıyla), ancak
gruplar arasında BMP15 ve GDF9 genlerine ait mRNA transkript
seviyesinde istatistiksel olarak bir fark gözlenmedi (p>0.05). Sonuç olarak intraovarian genlerin farklı
ekspresyonunun, ovaryum dokusunu oluşturan hücre popülasyonu içinde folikül
dinamikleri ve gen ekspresyon seviyelerinin farklılıklarıyla ilişkili
olabileceğini ve bu durumun da foliküler ve luteal dönemin sonucu olarak ortaya
çıkabileceği düşünülmektedir. 

Keywords

Korpus luteum, holştayn, preovulatör folikül, , intraovarian genler, qRT-PCR

Expression Profile of Intraovarian Genes in Ovary Tissues at Follicular and Luteal Phases in Holstein Cattle

Abstract

The aim of the
present study is to determine comparatively expression levels of the BMP15, TGFB1, TGFB2 and GDF9 genes in the preovulatory follicle
and corpus luteum tissue of Holstein cattle. For this purpose, primarily the
tissues were examined by immunohistochemical staining. Later on, follicular
fluid was analyzed by ELISA test and estradiol and progesterone levels were
determined. Finally, expression levels of the related genes were determined
between the groups by qRT-PCR. Immunohistochemical staining revealed that
estrogen receptor alpha and progesterone receptor immunoreactivities were
intensely present in preovulatory follicles, estrogen alpha receptor
immunoreactivity was very slight and progesterone receptors were similar to
positivity in preovulatory follicles in corpus luteum. Furthermore, estradiol
level was high in preovulatory follicles and progesterone level was high in
corpus luteum. The levels of mRNA transcripts of the TGFB1 and TGFB2 genes in
the preovulatory follicles were statistically higher than the corpus luteum (p<0.01, p<0.05, respectively), but
there was no statistically significant difference between the groups in the
mRNA transcript levels of the BMP15
and GDF9 genes (p>0.05). As a result, it is thought that differential expression
of intraovarian genes may be associated with differences in follicular dynamics
and gene expression levels within the cell population of ovarian tissue, so
this situation may result in follicular and luteal phase.

Keywords

Corpus luteum, holstein, intraovarian genes, preovulatory follicle, qRT-PCR

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