Kısa Süreli Saklanan Koç Spermasında 7-Dehidrokolesterolün Spermatolojik Parametrelere Etkisi

Year 2019, Volume: 12 Issue: 1, 15 - 19, 31.03.2019

Muhammed Enes İnanç Şükrü Güngör Ayhan Ata

https://doi.org/10.30607/kvj.492556

Abstract

Bu çalışmanın
amacı, 7-dehidrokolesterol yüklenmiş siklodekstrinin (7-DCLC) +4 C⁰’de kısa süreli saklanan koç spermasının spermatolojik
parametrelerine etkisinin araştırılmasıdır. Her bir
hayvandan spermalar elektroejakülatör yardımı ile sezon dışında alınarak dört eşit gruba ayrıldı, farklı oranlarda 7-DCLC (1,5 mg, 3,0 mg and 5
mg/120x10⁶)  içeren ve
içermeyen kontrol (C) tris/yumurta sarısı sulandırıcı ile sulandırıldı.
Sulandırılan spermalar, +4 C⁰’de saklanarak 96 saat süresince motilite, membran bütünlüğü ve anormal
spermatozoa yönünden incelendi. Saklama süresi boyunca anormal spermatozoa
yönünden gruplar arasında bir farklılık bulunmazken (P>0,05); motilite
açısından gruplar arasında farklılıklar tespit edildi (P<0,05).  72. ve 96. zaman dilimlerinde en yüksek
motilite 7-DCLC 1,5 mg/120x10⁶ grubunda tespit edildi (P<0,05).
En yüksek dozlu (5 mg) 7-DCLC grubu diğer gruplarla karşılaştırıldığında
spermatozoa motilitesini ve membran bütünlüğünü düşürdüğü tespit edildi.
Ayrıca, 72. saatte 7-DCLC 3,0 mg grubunun membran bütünlüğü kontrol grubuna
göre daha az olduğu tespit edildi (P<0,05). Sonuç olarak, 7-DCLC 1,5 mg
içeren tris/yumurta sarısı sulandırıcısı koç spermasında motiliteyi ve membran
bütünlüğünü +4 C⁰’de 96 saat süresince koruduğu belirlendi.

Keywords

7-dehidrokolesterol, siklodekstrin, motilite, membran bütünlüğü, kısa süreli saklama

Effect of 7-Dehydrocholesterol on Ram Semen Spermatological Parameters on Short Term Preservation

Abstract

The aim of this study
was investigated to effect of 7-dehydrocholesterol loaded cyclodextrin (7-DCLC) on ram semen
spermatological parameters on short term preservation at +4 ⁰C. Collected semen with electroejaculator at non-breeding
season. Each animal native ejaculates were mixed and divided into 4 equal groups, diluated with tris/egg yolk extender
with included different rates of 7-DCLC (1.5 mg, 3.0 mg and 5 mg/120x10⁶)
and control (C). After extended semen samples were preservation at +4 ⁰C and evaluated motility, membrane integrity and
abnormal spermatozoa rate until 96. hour. There were no significant differences
between the groups on abnormal spermatozoa rate (P>0.05); there were a
significant differences between the groups on motility at all storage period
(P<0.05).The highest motility were observed in 7-DCLC 1.5 mg at 72. and 96.
hour (P<0.05).  The highest dose (5
mg/120x10⁶) 7-DCLC were decreased the motility and membrane
integrity compared to other groups. Besides, 7-DCLC 3.0 mg group membrane
integrity was lower than control at 72. hour (P<0.05). In conclusion, tris/egg yolk extender which is contain 7-DCLC 1.5 mg
were protected to ram semen motility and membrane integrity up to 96. hour at
+4°C preservation. 

Keywords

7-dehydrocholesterol, cyclodextrins, motility, membrane integrity, short term storage

References

• Aksoy M, Akman O, Lehimcioğlu NC, Erdem H. Cholesterol-loaded cyclodextrin enhances osmotic tolerance and inhibits the acrosome reaction in rabbit spermatozoa. Anim Reprod Sci. 2010; 120: 166-172.
• Amorim EAM, Graham JK, Spizziri B, Meyers M, Torres CA. Effect of cholesterol or cholesteryl conjugates on the cryosurvival of bull sperm. Cryobiology. 2009; 58: 210-214.
• Avdatek F, Yeni D, Gündoğan M. Merinos koçlarda spermaya katılan antioksidanların kısa süreli saklama sırasında spermatolojik parametreler ve DNA hasarı üzerine etkileri. Kocatepe Vet J. 2018; 11: 126-133.
• Bailey JL, Bilodeau JF, Cormier N. Semen cryopreservation in domestic animals: a damaging and capacitating phenomenon. J Androl. 2000; 21: 1–7.
• Benhenia K, Rahab H, Smadi MA, Benmakhlouf H, Lamara A, Idres T, Iguer-Ouanda M. Benifical and harmful effects of cyclodextrin- vitamin E complex on cryopreserved ram sperm. Anim. Reprod. Sci. 2018; 195; 266-273.
• Bucak MN, Atessahim A, Yüce A. Effect of anti-oxidants and oxidativestress parameters on ram semen after the freezing-thawing process. Small Rum Res. 2008; 75: 128–134.
• Crini G. A history of cyclodextrins. Chemical reviews. 2014; 114: 10940-10975.
• De Lamirande E, Leclerc P, Gagnon C. Capacitation as a regulatory event that primes spermatozoa for the acrosome reaction and fertilization. Mol Hum Reprod. 1997; 3: 175–194.
• Falchia L, Gallerib G, Zeddaa MT, Paua S, Boglioloa L, Ariua F, Leddaa S. Liquid storage of ram semen for 96 h: Effects on kinematic parameters, membranes and DNA integrity, and ROS production. Livestock Science. 2018; 207: 1–6.
• Hancock JL. The morphology of bull spermatozoa. J Exp Biol. 1952; 29: 445-453.
• Inanc ME, Tekin K, Olgac KT, Ozen D, Stelletta C, Uysal O, Daskin A. Effects of 7-dehydrocholesterol and cholesterol-loaded cyclodextrins on bull sperm motility during short term storage. Kafkas Univ Vet Fak Derg. 2017; 23: 661-664.
• Inanc ME, Uysal O, Ata A. Cryopreservation and evalution of Akkaraman ram semen with 7-dehydrocholesterol. Ankara Univ Vet Fak Derg. 2018a; 65: 179-185.
• Inanc ME, Tekin K, Olgac KT, Yilmaz B, Cil B, Tasdemir U, Tuncer PB, Buyukleblebici S, Durmaz E, Uysal O. Effect of cholesterol loaded cyclodextrin on semen cryopreservation of Aksaray Malakli shepherd dogs of different ages. Animal Reprod Sci. 2018b; 193, 191-200.
• Inoue K, Kubota S, Seyama Y. Cholestanol induces apoptosis of cerebellar neuronal cells, biochemical and biophysical. Biochemical and Biophys Res Commun. 1999; 256: 198-203.
• Katanbegzadeh H, Farid B, Mohammadreza T. Cryoprotectant-free freezing of the goat epididymal sperm. Cryoletters. 2014; 35: 293-298.
• Khan J, Tahir MZ, Khalid A, Sattar A, Ahmad N. Effect of cholesterol‐loaded cyclodextrins on cryosurvival of dog spermatozoa. Reprod Domest Anim. 2017; 52: 265-268.
• Lopez-Revuelta A, Sanchez-Gallego JI, Garcia-Montero AC, Hernández-Hernández A, Sánchez-Yagüe J, Llanillo M. Membrane cholesterol in the regulation of aminophospholipid asymmetry and phagocytosis in oxidized erythrocytes. Free Radical Bio Med. 2007; 42: 1106-1118.
• Mansour M. Modification of hypo-osmotic swelling test to evaluate the integrity of stallion sperm plasma membrane. Global Veterinaria. 2009; 3: 302-307.
• Maxwell WMC, Watson PF. Recent progress in the preservation of ram semen. Anim Reprod Sci. 1996; 42: 55–65.
• Maxwell WM, Evans G, Mortimer ST, Gillan L, Gellatly ES, McPhie CA. Normal fertility in ewes after cervical insemination with frozen-thawedspermatozoa supplemented with seminal plasma. Reprod Fertil Dev. 1999; 11: 123–126.
• Medeiros CMO, Forell F, Oliveria ATD, Rodrigues JL. Current status of sperm cryopreservation: why isn’t it better? Theriogenology. 2002; 57: 327-344.
• Moce E, Blanch E, Tomás C, Graham J K. Use of cholesterol in sperm cryopreservation: present moment and perspectives to future. Reprod Domest Anim. 2010; 45: 57-66.
• Moraes EA, Graham JK, Torres CA, Meyers M, Torres CA. Delivering cholesterol or cholestanol to bull sperm membranes improves. cryosurvival. Anim Reprod Sci.2010; 118: 148-54.
• Murphy C, English AM, Holden SA, Fair S. Cholesterol-loaded-cyclodextrins improve the post-thaw quality of stallion sperm. Anim Reprod Sci. 2014; 145: 123-129.
• O’Hara L, Hanrahan JP, Richardson L, Donovan A, Fair S, Evans, ACO., Lonergan P. Effect of storage duration, storage temperature, and diluent on the viability and fertility of fresh ram sperm. Theriogenology. 2010; 73: 541–549.
• Oettle EE. Sperm morphology and fertility in the dog. J Reprod Fertil. 1993; Supplement 47: 257-260.
• Oliveira RR, Rates DM, Pugliesi G, Ker PG, Arruda RP, Moraes EA, Carvalho GR. Use of Cholesterol‐Loaded Cyclodextrin in Donkey Semen Cryopreservation Improves Sperm Viability but Results in Low Fertility in Mares. Reprod Domest Anim. 2014; 49: 845-850.
• Partyka A, Strojecki M, Niżański W. Cyclodextrins or cholesterol-loaded-cyclodextrins? A better choice for improved cryosurvival of chicken spermatozoa. Anim Reprod Sci. 2018; 193: 235-244.
• Paulenz H, Soderquist L, Perez-Pe R, Berg KA. Effect of different extenders and storage temperatures on sperm viability of liquid ram semen. Theriogenology. 2002; 57: 823–36.
• Purdy PH, Graham JK. Effect of cholesterol-loaded cyclodextrin on the cryosurvival of bull sperm. Cryobiology. 2004; 48: 36-45.
• Revell SG, Mrode RA. An osmotic resistance test for bovine semen. Anim Reprod Sci. 1994; 36: 77-86.
• Salamon S, Maxwell WMC. Frozen storage of ram semen. 2. Causes of low fertility after cervical insemination and methods of improvement. Anim Reprod Sci. 1995; 38:1–36.
• Salamon S, Maxwell WM. Storage of ram semen. Anim Reprod Sci. 2000; 62: 77–111.
• Schafer S, Holzmann A. The use of transmigration and spermac stain to evaluate epididymal cat spermatozoa. Anim Reprod Sci. 2000; 59: 201-11.