Investigation of Cytotoxic Effects of Curcuma Longa, Zingiberaceae and Dianthus Caryophllus, Which are Commonly Used as Food Supplements in Daily Life

Year 2019, Volume: 12 Issue: 3, 351 - 356, 30.09.2019

Özgür Albuz

https://doi.org/10.30607/kvj.593514

Cited By: 1

Abstract

The aim of this study
is to investigate the cytotoxic effects, which have an important place ,in
terms of public health as using herbal food supplement, of ginger, turmeric and
clove seeds over healthy cells. In this study, designed as an in vitro cell
culture study, fibroblast cells were used to investigate the effect of plant
extracts on healthy cells. MTT cell viability test was used to determine
cytotoxicity. The MTT test was performed according to ISO 10993-5 standards and
when the cell viability was calculated to be below 70% in the MTT method, the
sample was considered potential cytotoxic. When the viability values calculated
according to the absorbance values obtained from MTT test are examined; It was
calculated that ginger and turmeric extracts were not cytotoxic at any
concentration, including the highest concentrations. On the other hand, it was
calculated that 1: 2 concentration of clove extract was cytotoxic and the
viability was 26.7%. As a result of this study, it has been found that clove
use is remarkable in terms of plant nutritional supplements with its current
use in terms of cytotoxic effect compared to turmeric and ginger. 

Keywords

Curcuma Longa, Zingiberaceae, Dianthus Caryophllus, Cytoxicity

Günlük Yaşamda Gıda Takviyesi Olarak Kullanılan Zencefil, Zerdeçal ve Karanfilin Sitotoksik Etkilerinin Araştırılması

Abstract

Bu çalışmanın amacı,
günlük yaşamda gıda takviyesi olarak kullanılan ve halk sağlığı açısından
önemli bir yeri olan zencefil, zerdeçal ve karanfil tohumlarının sağlıklı
hücreler üzerinde sitotoksik etkilerini araştırmaktır. İn vitro hücre kültürü
çalışması olarak tasarlanan bu çalışmada, bitki özlerinin sağlıklı hücreler
üzerindeki etkisini araştırmak için fibroblast hücreleri kullanılmıştır.
Sitotoksisiteyi belirlemek için MTT hücre canlılığı testi kullanılmıştır. MTT
testi ISO 10993-5 standartlarına göre yapıldı ve MTT yönteminde hücre canlılığı
% 70'in altında olduğu hesaplandığında, numunenin sitotoksik potansiyelde
olduğu kabul edilmiştir. MTT
testinden elde edilen absorbans değerlerine göre hesaplanan canlılık değerleri
incelendiğinde; Zencefil ve zerdeçal ekstrelerinin, en yüksek konsantrasyonlar
dahil olmak üzere herhangi bir konsantrasyonda sitotoksik olmadığı
hesaplanmıştır. Öte yandan, 1:2 oranında karanfil ekstresi konsantrasyonunun
sitotoksik ve canlılığın % 26.7 olduğu hesaplanmıştır. Bu çalışma sonucunda, özellikle karanfil kullanımının zerdeçal ve
zencefil ile karşılaştırıldığında sitotoksik etkiye sahip olması bakımından
mevcut kullanımıyla, bitkisel gıda takviyeleri açısından dikkate değer olduğu
tespit edilmiştir.

Keywords

Zerdeçal, zencefil, karanfil, sitotoksitite

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