Farklı Üreme Yaşındaki Keçilerde Dondurulmuş Sperma ile Servikal Tohumlama

Year 2019, Volume: 12 Issue: 3, 357 - 362, 30.09.2019

Koray Tekin

https://doi.org/10.30607/kvj.604959

Abstract

Keçide dondurulmuş sperma ile servikal suni
tohumlama (ST) uygulaması düşük gebelik oranları nedeniyle halen sınırlıdır. Bu
çalışmanın amacı iki farklı üreme yaşındaki Saanen keçilerinde donmuş sperma
kullanımının etkinliğini belirlemekti. Senkronizasyon ve gebelik oranlarının
etkileri de incelenmiştir. Doksan sekiz (98) dişi Saanen keçisi iki gruba
ayrıldı: Grup-1 = 49 (7 aylık) ve Grup-2 = 49 (19 aylık) keçi. Östrus 11 gün süresince
intravajinal sünger (30 mg;  fluogestone
acetate, FGA)
ve süngerin çıkarılmasından 48 saat önce 50 ug kloprostenol (PGF2a) ve gebe
kısrak serum gonadotropini (PMSG, 500 IU) ile senkronize edildi. Sabit zamanlı
servikal tohumlama dondurulmuş sperma ile üreme mevsiminde süngerlerinin
çıkarılmasından 43-45 saat sonra gerçekleştirildi. Östrus arama tekesi ile
tespit edildi. Grup 1 ve Grup 2'de östrus görülme oranları sırasıyla %71.4,
%63.3 olarak tespit edildi (p>0.05). Altmışaltı (66) keçi dondurulmuş-çözdürülmüş
spermayla tohumlandı. Gebelik abdominal ultrasonografi ile belirlendi. Gebelik
oranları (Grup-1: %31.4, Grup-2: %32.3) yaş grupları arasında anlamlı bir
farklılık göstermedi (p>0.05). Ayrıca, artan çoklu doğum ve azalan
embriyonik kayıplar sayesinde Grup-1’de (%145.5), Grup-2’ye (%90.0) göre daha
yüksek oranda yavru elde edilmiştir. Sonuç olarak, Saanen keçileri, 7 aylık
yaştan itibaren suni tohumlama programlarına alınabilir ve yüksek
senkronizasyon başarısı ve yavru verimine gibi birçok fayda sağlayabilir.

Keywords

Dondurulmuş sperma, fertilite, gebelik, üreme yaşı, suni tohumlama

Cervical Insemination with Frozen Thawed Semen in Goats at Different Breeding Age

Abstract

Artificial insemination (AI) in goat is currently
limited by the poor fertility obtained following the cervical insemination with
frozen-thawed semen. The objective of the present study was to determine the
efficiency of frozen semen use in two different breeding ages at Saanen goats.
The effects of synchronization and fertility rates were also examined.
Ninety-eight (98) Saanen goats were divided into two groups: Group-1 = 49
(7-months-old) and Group-2 = 49 (19-month- old) does. Estrus was
synchronized by impregnated intravaginal sponges (30 mg; fluogestone
acetate,
FGA), and i.m. administration of 50 µg of cloprostenol (PGF2α) and
pregnant mare serum gonadotropin (PMSG, 500 IU), at 48 h before
sponge removal. Cervical fixed time AI was carried out with frozen semen
43 h after removal of the sponges during the breeding season. Estrus was
detected by teaser buck. The occurrence of estrus in Group-1 and Group-2 were
71.4%, 63.3%, respectively (p>0.05). Sixty-six (66) goats were inseminated
with frozen‐thawed semen. The pregnancy rate was determined by
abdominal scanning. Pregnancy rates were (Group-1: 31.4%, Group-2: 32.3%) not
differed significantly between age groups (p>0.05). Besides, higher lambing
rates were obtained in Group-1 (145.5%) than in Group-2 (90.0%) due to
increased prolificacy and reduced embryonic failure. In conclusion, Saanen goats in younger ages, starting
from the 7 months old age could be included in the breeding programs and could
provide several benefits such as higher percentages of synchronization success
and prolificacy. 

Keywords

Artificial Insemination, breeding age, frozen semen, pregnanc, fertility

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