Türkiye'de Subklinik Mastitisli Pırlak Koyunlardan İzole Edilen Stafilokoklarda Metisilin Direnç ve Panton-Valentine Lökosidin Genlerinin Prevalansı

Year 2019, Volume: 12 Issue: 4, 424 - 429, 31.12.2019

Esra Şeker Erhan Özenç Duygu Baki Acar Müesser Yılmaz

https://doi.org/10.30607/kvj.617025

Cited By: 3

Abstract

Bu çalışmada,
subklinik mastitis yönünden taranan 235 Pırlak koyuna ait 464 meme lobu süt
örneğinden daha önce izole edilen 47 Stafilokok türünde mecA ve pvl genlerinin
varlığının araştırılması amaçlandı. Çalışmada, Pırlak koyunlardan izole edilen
13 S. aureus, 13 S. epidermidis, altı S.
xylosus, beş S. chromogenes, üç S. simulans, üç S. hyicus, iki S. warneri,
bir S. lentus ve bir S. saprophyticus suşu kullanıldı. Toplam
10 suşun (%21,3) mecA geni taşıdığı,
bunlardan ikisinin (%4,2) ayrıca pvl
genine de sahip olduğu belirlendi. mecA
geni taşıyan suşlar S. aureus (3/13),
S. xylosus (3/6), S. epidermidis (2/13), S. lentus (1/1) ve S. hyicus (1/3) olarak bulundu. Toplam sekiz suşta (%17,0) pvl geni belirlenirken, bunlardan
altısının (%12,8) bu geni tek başına taşıdığı tespit edildi. pvl pozitif suşların üçü S. aureus, üçü S. xylosus, biri S. simulans
ve biri S. hyicus olarak belirlendi.
Bilgimize göre, bu çalışma Türkiye'de subklinik mastitisli Pırlak koyunlardan
izole edilen Stafilokoklarda mecA ve pvl genlerinin varlığını gösteren ilk
çalışmadır. 

Keywords

Koyun, Mastitis, Metisilin Direnci, Panton-Valentine Lökosidin, Stafilokok

Prevalence of Methicillin Resistance and Panton-Valentine Leukocidin Genes in Staphylococci Isolated from Pirlak Sheep with Subclinical Mastitis in Turkey

Abstract

This study aimed to
investigate the presence of mecA and pvl genes in 47 Staphylococci previously
isolated from 464 half-udder milk samples belong to 235 Pirlak sheep screening
for subclinical mastitis. The species from Pirlak sheep used in the present
study included: 13 S. aureus, 13 S. epidermidis, six S. xylosus, five S.
chromogenes, three S. simulans,
three S. hyicus, two S. warneri, one S. lentus and one S.
saprophyticus. A total of 10 strains (21.3%) were determined to harbour mecA gene, of these, two (4.2%) also
contained pvl gene. The strains
carrying mecA gene were found to be S. aureus (3/13), S. xylosus (3/6), S. epidermidis
(2/13), S. lentus (1/1) and S. hyicus (1/3). The presence of pvl gene was determined in a total of
eight strains (17.0%), six (12.8%) of these were alone. Of pvl positive strains, three, three, one, and one were S. aureus, S. xylosus, S. simulans
and S. hyicus, respectively. To our
knowledge, this is the first study showing the presence of mecA and pvl genes in the
Staphylococci isolated from Pirlak sheep with subclinical mastitis in Turkey. 

Keywords

Mastitis, Methicillin Resistance, Panton-Valentine Leukocidin, Sheep, Staphylococci

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