Morphologic and Molecular Detection of Dactylogyrus Species in Cyprinus Carpio and Ctenopharyngodon Idella in Southwestern Iran

Year 2020, Volume: 13 Issue: 3, 241 - 247, 30.09.2020

Mohammad Hossein Razi Jalali Sara Larki Rahim Peyghan Maryam Bastami

https://doi.org/10.30607/kvj.723034

Abstract

Monogeneans gill parasites of the diversified Dactylogyrus genus are host-specific to freshwater fish of the Cyprinidae family. Accurate detection of various Dactylogyrus species is very time-consuming and requires highly experienced personnel; to overcome this problem, the genomic DNA technology has been recently used to detect Dactylogyrus spp. In this study, 23 Cyprinus carpio and 19 Ctenopharyngodon idella isolates were collected from different farms and local markets of Ahvaz, southwest of Iran. The gill arches of each fish were carefully examined under a light microscope. Dactylogyrus species was identified morphologically. Identical species were kept in ethanol for molecular study. Molecular analysis was performed using amplification of the ITS-1 region of the ribosomal RNA gene of the parasite. Then, the amplified PCR products were sequenced. The aligned nucleotide sequences were analyzed to construct the phylogenetic tree of the identified species. The results revealed two lineages including D. extensus isolated from common carp and D. lamellatus taken from grass carp fish. Phylogenetic analysis showed that the detected D. extensus and D. lamellatus isolates from Khuzestan region were clustered with some Iranian (Guilan), Chinese and Czech isolates. Importing fingerlings from Guilan Province could lead to introducing monogenean infections to native cyprinid fish.

Keywords

Dactylogyrus, Common Carp, Grass carp, Fish, PCR

Supporting Institution

The Research Council of Shahid Chamran University of Ahvaz, Iran

Project Number

Master thesis

İran'ın Güneybatısında Yer Alan Cyprinus Carpio ve Ctenopharyngodon İdella'daki Dactylogyrus Türlerinin Morfolojik ve Moleküler Tespiti

Abstract

Çeşitlendirilmiş Dactylogyrus cinsinin solungaç monojenleri, Cyprinidae ailesinin tatlı su balıklarına ev sahipliğine özgüdür. Çeşitli Dactylogyrus türlerinin doğru tespiti çok zaman alıcı olduğundan ve çok deneyimli personel gerektirdiğinden, genomik DNA teknolojisi son zamanlarda Dactylogyrus spp. Bu çalışmada, Güneybatı İran'ın Ahvaz'ın farklı çiftliklerinden ve yerel pazarlarından 23 Cyprinus carpio ve 19 Ctenopharyngodon idella toplandı. Her balığın solungaç kemerleri, ışık mikroskobu altında dikkatle incelenir. Dactylogyrus türlerinin morfolojik olarak tanımlanması morfolojik olarak belirlenmiştir. benzer türler moleküler çalışma için etanol içinde tutuldu. Moleküler analiz, parazitin ribozomal RNA geninin ITS-1 bölgesinin amplifikasyonu kullanılarak yapıldı. Daha sonra amplifiye PCR ürünleri dizildi. Hizalanmış nükleotit sekansları, tanımlanan türlerin filogenetik ağacını yürütmek için analiz edilir. Sonuçlar, ortak sazandan izole edilen D. extensus ve çim sazan balıklarından alınan D. lamellatus da dahil olmak üzere iki soyun ortaya çıktığını göstermiştir. Filogenetik analiz, Khuzestan bölgesinden tespit edilen D. extensus ve D. lamellatus izolatlarının bazı İran (Guilan), Çin ve Çek Cumhuriyeti izolatlarıyla kümelendiğini gösterdi. Guilan Eyaletinden balıkların ithal edilmesi, yerli Cyprinid balıklarına monojenik enfeksiyonların ortaya çıkmasına neden olabilir.

Keywords

Sazan, Çim Sazan, Balık, PCR

Project Number

Master thesis

References

• Ahmadi A, Borji H, Naghibi A, Nasiri MR, Sharifiyazdi H. Morphologic and molecular (28S rDNA) characterization of Dactylogyrus spp. in Cyprinus carpio and Ctenopharyngodon idella in Mashhad, Iran. Can J Vet Res. 2017; 81(4): 280–284.
• Borji H, Naghibi A, Nasiri M, Ahmadi A. Identification of Dactylogyrus spp. and other parasites of common carp in northeast of Iran. JOPD. 2012; 36: 234–238.
• Daghigh Roohi JD, Dalimi A, Pourkazemi M, Ghasemi M. Morphometric and molecular characterization of Dactylogyrus lamellatus isolated from farmed grass carp, Ctenopharyngodon idella (Valenciennes, 1844), in Guilan province, Iran. Iran J Fish Sci. 2019; 1-11.
• Gibson DI, Timofeeva TA, Gerasev PI. A catalogue of the nominal species of the monogenean genus Dactylogyrus Diesing, 1850 and their host genera. Syst Parasitol. 1996; 35: 3–48.
• Gusev AV. Class Monogenea, In: Keys to Parasites of the Freshwater Fish Fauna of the USSR. (Vol. 2: Parasitic Metazoa), Ed; Bauer ON, Nauka, Leningrad. 1985; pp. 15-251. [in Russian]
• Jalali, B. Parasites and parasitic diseases of freshwater fishes of Iran, 1st Ed, Aquaculture Deputy, Fisheries Company, Iran. 1998; p. 564. [In Persian].
• Jalali B, Barzegar M. Dactylogyrus spp. (Monogenea: Dactylogyridae) in common carp (Cyprinus carpio L, 1750) of fresh water fishes of Iran and description of the pathogenicity of D.sahuensis Ling, 1985. J Agr Sci Tech. 2005; 7: 9-16.
• Kadlec D, Simkova A, Gelnar M. The microhabitat distribution of two Dactylogyrus species parasitizing the gills of the barbel, Barbus barbus. J Helminthol. 2003; 77(4): 317-325.
• Kimura M. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J Mol Evol. 1980; 16 (2): 111-120.
• Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Mol Biol Evol. 2018; 35: 1547-1549.
• Lu C, Zhang HY, Ji J, Wang GX. In vivo anthelmintic activity of Dryopteris crassirhizoma, Kochia scoparia, and Polygala tenuifolia against Dactylogyrus intermedius (Monogenea) in goldfish (Carassius auratus). Parasitol Res. 2012; 110(3): 1085-1090.
• Mahdipour M, Barzegar M, Jalali B. A survey on monogenean parasites of gills of fishes in Zayandeh-Rud river. Iran J Vet Sci. 2004; 1: 19-28. [In Persian].
• Molnar K. Studies on gill parasitosis of the grass carp (Ctenopharyngodon idella) caused by Dactylogyrus lamellatus Achmerow, 1952. I. Morphology and biology of Dactylogyrus lamellatus. Acta Vet Acad Sci Hung. 1971; 21(2): 267-289.
• Mozhdeganlou Z, Ebrahimzadeh Mousavi H, Shayan P, Soltani M, Ebrahimzadeh E, Rostami M. Detection of single Dactylogyrus spp. in DNA extracted from infected gill tissue of fishes using Polymerase Chain Reaction. Int J Vet Res. 2011; 5(2): 77-80.
• Olson PD, Cribb TH, Tkach VV, Bray RA, Littlewood DTJ. Phylogeny and classification of the Digenea (Platyhelminthes: Trematoda). Int J Parasitol. 2003; 33: 733-755.
• Shamsi S, Jalali B, Aghazadeh Meshgi M. Infection with Dactylogyrus spp. among introduced cyprinid fishes and their geographical distribution in Iran. J Vet Res. 2009; 10: 70-74.
• Sharma P, Agarwal N, Kumar S. Ribosomal DNA and morphological analysis of Dactylogyrus species from freshwater fishes of India. J Parasitic Dis. 2011; 35(2): 210–214.
• Simkova A, Pecinkova M, Rehulkova E, Vyskocilova M. Dactylogyrus species parasitizing European Barbus species: morphometric and molecular variability. Parasitology. 2007; 134(12): 1751-1765.
• Strona G, Stefani F, Galli P. Field preservation of monogenean parasites for molecular and morphological analyses. Parasitol Int. 2009; 58: 51-54.
• Thoney DA, Hargis WJ. Monogenea (platyhelminthes) as hazards for fish in confinement. Annu Rev Fish Dis. 1991; 1: 133-153.
• Tu X, Ling F, Huang A, Wang G. The first report of Dactylogyrus formosus Kulwiec, 1927 (Monogenea: Dactylogyridae) from goldfish (Carassius auratus) in central China. Parasitol Res. 2015; 114(7): 2689-96.
• Woo P, Bruno D, Lim L. Diseases and disorders of finfish in cage culture, CABI, Wallingford, Oxfordshire. 2002; p. 353.
• Woo PTK. Fish diseases and disorders. Vol. 1, protozoan and metazoan infections, 2nd Ed, CABI, Wallingford, Oxfordshire. 2006; p. 791.
• Zolfinejad K, Khara H, Filizadeh Y. Food preference and growth of grass carp, Ctenopharyngodon idella (Cuvier and Valenciennes, 1844) fed some aquatic and terrestrial plants. Iran J Fish Sci. 2017; 16(4): 1278-1286.