Influence of Proanthocyanidin on Motility and Osmotic Resistance Parameters of Merino Ram Sperm During Short Term Storage

Year 2020, Volume: 13 Issue: 4, 362 - 367, 31.12.2020

Fatih Avdatek , Deniz Yeni , Umut Taşdemir

https://doi.org/10.30607/kvj.800186

Abstract

The activity of proanthocyanidin oligomers is approximately 50 times greater than that of vitamin C and vitamin E in terms of antioxidant action. The aim of this study is to investigate the effects of different doses proanthocyanidin adding to extender on ram sperm motility, membran integrity and viability test, during liquid storage of ram semen. Ejaculates were collected from four Merino rams using an electroajaculator once a week and this process was repeated six times in non-breeding season. Ejaculates were split into four aliquots and diluted to a final concentration of 150x106 spermatozoa/ml with the base extender containing proanthocyanidin (10, 50 and 100 µg/ml) and no additive (control). Diluted semen samples were transferred and stored at +4°C and sperm motility, membrane integrity and viability was analysed in determined intervals during three days storage period. Motility was evaluated subjectively by a phase contrast microscopy at 37°C. Membrane integrity and viability was analysed in hypo-osmotic resistance test with eosin staining test. After 24, 48 and 72 h, 10 μg / ml proanthocyanidin treatment sperm samples presented higher motility and HOST/E test results than control group (P <0.05) in ram semen stored at +4°C. In conclusion, it was determined that the doses of 10 μg / ml of proanthocyanidin added to extender in +4 0C storage of ram sperm showed a protective effect compared to the control group.

Keywords

Ram semen, Proanthocyanidin,, Motility,, Short term storage, Osmotic resistance

Kısa Süreli Saklanan Merinos Koç Spermasında Proantosiyanidin Motilite ve Ozmotik Direnç Parametreleri Üzerine Etkisi

Abstract

Proantosiyanidin oligomerlerinin aktivitesi, antioksidan etki açısından C vitamini ve E vitaminine göre yaklaşık 50 kat daha fazladır. Bu çalışmanın amacı, koç spermasının kısa süreli saklanmsı sırasında, farklı dozlardaki sulandırıcıya eklenen proantosiyanidin, koç spermatozoon motilitesi, membran bütünlüğü ve canlılığı üzerine etkilerini araştırmaktır. Ejakülatlar, sezon dışında dört Merinos koçtan elektroajakülatör yardımıyla haftada bir kez toplandı ve bu işlem altı kez tekrarlandı. Ejakülatlar dört eşit parçaya bölünerek proantosiyanidin (10, 50 ve 100 ug / ml) içeren ve hiçbir katkı maddesi içermeyen (kontrol) sulandırıcılar ile nihai ml’de 150x106/ml spermatozoa olacak şekilde sulandırıldı. Sulandırılan sperma örnekleri + 4 ° C'de 72 saat süresince saklandı ve spermatozoa motilitesi, membran bütünlüğü ve canlılığı üç günlük saklama süresi boyunca değerlendirildi. Motilite, 37 ° C'de faz kontrast mikroskop ile sübjektif olarak değerlendirildi. Membran bütünlüğü ve canlılığı, eozin boyama testi ile hipoozmotik direnç testinde (HOST/E) değerlendirildi. 24, 48 ve 72 saat sonra, + 4 ° C'de saklanan koç spermasında 10 μg / ml proantosiyanidin ile muamele edilmiş spermatozoon örnekleri kontrol grubuna göre daha yüksek motilite ve HOST / Eosin test sonuçları göstermiştir (p <0,05). Sonuç olarak koç sperminin +4 oC'de saklanmasında sulandırıcıya eklenen 10 μg / ml proantosiyanidin kontrol ve diğer antioksidan gruplarına göre daha iyi bir koruyucu etki gösterdiği belirlendi.

Keywords

Koç sperması, Proanthocyanidin, Motilite, Kısa Süreli Saklama, Ozmotik Direnç

References

• Saloman S., Maxwell W.M.C. Storage of ram semen. Anim. Reprod. Sci., 2000; 62 (1-3): 77-111.
• Johnson L.A., Weıtze K.F., Fıser P., Maxwell W.M.V. Storage of boar semen. Anim Reprod Sci., 2000; 62: 143–172.
• Huo L.J., Yue K.Z., Yang Z.M. Characterization of viability, mitochondrial activity, acrosomal integrity and capacitation status in boar sperm during in vitro storage at different ambient temperatures. Reprod Fert Develop., 2002, 14: 509–514.
• Aitken R.J. Reactive oxygen species as mediators of sperm capacitation and pathological damage. Mol. Reprod. Dev., 2017, 84: 1039–1052.
• Gundogan M., Yeni D., Avdatek F., Fidan A.F. Influence of sperm concentration on the motility, morphology, membrane and DNA integrity along with oxidative stress parameters of ram sperm during liquid storage. Anim Reprod Sci., 2010, 122: 200-207.
• Zakošek P.M., Mrkun J., Nemec S.A., Zrimšek P. Improvement of liquid stored boar semen quality by removing low molecular weight proteins and supplementation with α-tocopherol. Anim. Reprod. Sci., 2017, 186: 52–61.
• Kasimanickam R., Kasimanickam V., Tibary A., Pelzer K. Effect of semen 344 extenders on sperm parameters of ram semen during liquid storage at 4°C. 345 Small Ruminant Res, 2011, 99: 208-213.
• Brillouet J., Fulcrand H., Carrillo S., Roumeas L., Romieu C. Isolation of native proanthocyanidins from grapevine (Vitis vinifera) and other fruits in aqueous buffer. J. Agric. Food Chem., 2017, 65: 2895–2901.
• Han M., Song P., Chang H., Rezaei A., Farrar S., Brown, M.A., Xi, M. Dietary grape seed proanthocyanidins (GSPs) improve weaned intestinal microbiota and mucosal barrier using a piglet model. Oncotarget, 2016, 7: 80313-80236.
• Bagchi D., Garg A., Krohn R.L., Bagchi M., Tran M.X., Stohs S.J. Oxygen free radical scavenging abilities of vitamins C and E, and a grape seed proanthocyanidin extract in vitro. Research Communications in Molecular Pathology and Pharmacology, 1997, 95(2): 179-189.
• Nandakumar V., Singh T., Katiyar S.K. Multi-targeted prevention and therapy of cancer by proanthocyanidins. Cancer Letters, 2008, 269.
• Garavaglia J., Markoski M.J., Oliveira A., Marcadenti A. Grape Seed Oil Compounds: Biological and Chemical Actions for Health. Nutrition and Metabolic Insights, 2016, 9: 59-64.
• Bagchi D., Bagchi M., Stohs S.J., Das D.K., Ray S.D., Kuszynski C.A., Joshi S.S., Pruess H.G. Free radicals and grape seed proanthocyanidin extract: importance in human health and disease prevention. Toxicol, 2000, 148: 187–197.
• Nunes M.A., Pimentel F., Costa A.S.G., Alvers R.C., Oliveria M.B.P.P. Cardioprotective properties of grape seed proanthocyanidins: An update. Trends in Food Science & Technology, 2016, 57: 31-39.
• Day A.P., Kemp H.J., Bolton C., Hartog M., Stansbie D. Effect of concentrated red grape juice consumption on serum antioxidant capacity and low – density lipoprotein oxidation. Ann Nutr Metab, 1997, 41: 353-357.
• Hafez, E.S.E. Semen Evaluation (5 Ed) In: Hafez, E. S. E. (Ed) Reproduction in farm animals. Philadelphia. Lea and Fabiger. 1987.
• Demirci E. Evcil hayvanlarda reprodüksiyon, Suni tohumlama ve androloji ders notları (1nd ed). F.Ü.Vet.Fak., 2002, Ders Teksiri No:53 Elazığ.
• Avdatek F., Gündoğan M. Effects of Some Antioxidant Additives on Spermatological Parameters, Oxidative Stress and DNA Damage After Freezing-Thawing Process in Ram Semen. F.U. Vet. J. Health Sci., 2018, 32 (2): 135–142.
• Bucak M.N., Tekin N. Protective effect of taurine, glutathione and trehalose on the liquid storage of ram semen. Small Ruminant Res, 2006, 73: 103-108.
• Tuncer P.B., Bucak M.N., Büyükleblebici S., Sarıözkan S., Yeni D., Eken A., Akalın P.P., Kinet H., Avdatek F., Fida A.F., Gündoğan M. The effect of cysteine and glutathione on sperm and oxidative stress parameters of post-thawed bull semen. Cryobiol, 2010, 61: 303-307.
• Avdatek F., Yeni D., Gündoğan M. Merinos Koçlarda Spermaya Katılan Antioksidanların Kısa süreli Saklama Sırasında Spermatolojik Parametreler ve DNA Hasarı Üzerine Etkileri. Kocatepe Vet J., 2018, 11(2): 126-133.
• Wen F., Li Y., Feng T., Du Y., Ren F., Zhang L., Han N., Ma S., Li F., Wang P., Hu J. Grape Seed Procyanidin Extract (GSPE) Improves Goat Sperm Quality When Preserved at 4 oC. Animals, 2019, 9: 810; doi:10.3390/ani9100810
• Li Q., Shaoyong W., Li Y., Chen M., Hu Y., Liu B., Yang G., Hu J. Effects of oligomeric proanthocyanidins on quality of boar semen during liquid preservation at 17 °C. Animal Reproduction Science, 2018, 98: 47–56.
• Evans R., Miller N.J., Paganga G. Structure –antioxidant activity relationships of flavonoids and phenolic acids. Free Radic Bio Med, 1996, 20: 933-956.
• Puiggròs F., Llópiz N., Ardévol A., Bladé C., Arola L., Salvadó M.J. Grape seed procyanidins prevent oxidative injury by modulating the expression of antioxidant enzyme systems. J. Agric. Food Chem., 2005, 53: 6080–6086.
• Al-Daraji H. The Use of Certain Grape Constituents for Improve Semen Quality and Storage Ability of Diluted Roosters' Semen. Am. J. PharmTech Res., 2012, 2(5): 308-322.
• Zhao Y., Gao L., Zhang H., Guo J., Guo P. Grape seed proanthocyanidin extract prevents DDP-induced testicular toxicity in rats. Food Funct., 2014, 5: 605–611.
• Long M., Yang S., Zhang Y., Li P., Han J., Dong S., Chen X., He J. Proanthocyanidin protects against acute zearalenone-induced testicular oxidative damage in male mice. Environ Sci Pollut Res., 2017, 24: 938–946.
• Su L., Deng Y., Zhang Y., Li C., Zhang R., Sun Y. Protective e_ects of grape seed procyanidin extract against nickel sulfate-induced apoptosis and oxidative stress in rat testes. Toxicol. Mech. Methods, 2011, 21: 487–494.
• Attia S.M., Bakheet A.S., Al-Rasheed N.M. Proanthocyanidins produce significant attenuation of doxorubicin-induced mutagenicity via suppression of oxidative stress. Oxidative Medicine and Cellular Longevity, 2010, 3(6): 404-413.