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Klinik Endometritisli İneklerde İntrauterin Kekik Esansiyel Yağı ile Birlikte Dimetil Sülfoksit Uygulamasının Klinik İyileşme Üzerine ve Serum Haptoglobin, Tümör Nekrozis Faktör ve Nitrik Oksit Düzeylerine Etkisi

Year 2021, Volume: 14 Issue: 1, 45 - 50, 31.03.2021
https://doi.org/10.30607/kvj.772613

Abstract

Bu çalışmada, intrauterin kekik yağı ile dimetil sülfoksit (DMSO) uygulamasının endometritisin klinik iyileşmesi, serum haptoglobin (Hp), tümör nekrozis faktör (TNF) ve nitrik oksit (NO) düzeylerine etkisini belirlemek amaçlandı. Çalışmada klinik endometritisli 90 inek rastgele 3 gruba ayrıldı. Birinci gruptaki ineklere (GI; n=30) kekik esansiyel yağı (20 mL), 12 mL DMSO ve 88 mL distile su karıştırıldı ve karışım (120 mL) intrauterin olarak verildi. İkinci gruptaki ineklere (GII; n=30) 20 mL kekik esansiyel yağı 100 mL distile su içerisine eklendi ve intrauterin olarak verildi. Son gruptaki ineklere ise (GIII; n=30) 12 mL DMSO, 108 mL distile su ile karıştırıldıktan sonra intrauterin olarak verildi. Tedaviden 14 gün sonra rektal, ultrasonografik ve vaginoskobik muayene yapıldı. Tüm hayvanlardan tedaviden hemen önce ve tedaviden 14 gün sonra kan alındı. Kan serumlarda Hp, TNF ve NO düzeyleri ELISA ticari kitleri ile belirlendi. Tüm gruplarda Hp, TNF ve NO düzeylerinin tedavi öncesine göre önemli oranda düştüğü saptandı. Klinik iyileşme oranı en yüksek GI’de (%93,3) tespit edildi. GII’de %73,3, GIII’te ise %56,6 olarak belirlendi. Sonuç olarak, intrauterin kekik esansiyel yağ tedavisi ile DMSO verilmesinin endometritisin klinik iyileşmesi üzerine pozitif yönde katkı sağladığı belirlenmiştir. 

Supporting Institution

Kafkas Üniversitesi Bilimsel Araştırmaları Projeleri Koordinatörlüğü

Project Number

2016-TS-47

References

  • Ahmadi MR, Derakhshandeh A, Shirian S, Daneshbod Y, Ansari-Lari M, Nazifi S. Detection of bacterial biofilm in uterine of repeat breeder dairy cows. Asian Pac. J. Reprod. 2017;6(3): 136-139.
  • Biswal SS, Das S, Balasubramanian S, Mohanty DN, Sethy K, Dasgupta M. Serum amyloid A and haptoglobin levels in crossbred cows with endometritis following different therapy. Vet. World. 2014;7(12): 1066–1070.
  • Çetin B, Çakmakçi S, Çakmakçi R. The investigation of antimicrobial activity of thyme and oregano essential oils. Turkish J. Agric. For. 2011;35(2): 145–154.
  • Chan JPW, Chu CC, Fung HP, Chuang S Te, Lin YC, Chu RM, Lee SL. Serum haptoglobin concentration in cattle. J. Vet. Med. Sci. 2004;66(1): 43–46.
  • Dubuc J, Duffield TF, Leslie KE, Walton JS, LeBlanc SJ. Randomized clinical trial of antibiotic and prostaglandin treatments for uterine health and reproductive performance in dairy cows. J. Dairy Sci. Elsevier; 2011;94(3): 1325–1338.
  • Erçetin E, Çetin Y. Effect of postpartum routıne ıntrauterıne treatment on fertılıty ın daıry cows. Van Vet. J. [Internet]. Van Veterinary Journal; 2020;(2): 60–66.
  • Fischer C, Drillich M, Odau S, Heuwieser W, Einspanier R, Gabler C. Selected pro-inflammatory factor transcripts in bovine endometrial epithelial cells are regulated during the oestrous cycle and elevated in case of subclinical or clinical endometritis. Reprod. Fertil. Dev. 2010;22(5): 818–829.
  • Gilbert RO. The effects of endometritis on the establishment of pregnancy in cattle. Reprod. Fertil. Dev. 2012;24(1): 252–257.
  • Hirvonen J, Huszenicza G, Kulcsàr M, Pyörälä S. Acute-phase response in dairy cows with acute postpartum metritis. Theriogenology. Elsevier Inc. 1999;51(6): 1071–1083.
  • Huzzey JM, Duffield TF, LeBlanc SJ, Veira DM, Weary DM, Von Keyserlingk MAG. Short communication: Haptoglobin as an early indicator of metritis. J. Dairy Sci. American Dairy Science Association. 2009;92(2): 621–625.
  • Kaçar C, Kaya S. İneklerde uterus enfeksiyonları ve üreme performansı üzerine etkisi. Kafkas Univ. Vet. Fak. Derg. 2014;20(6): 975–982.
  • Kaya S, Merhan O, Kacar C, Colak A, Bozukluhan K. Determination of ceruloplasmin, some other acute phase proteins, and biochemical parameters in cows with endometritis. Vet. World. 2016;9(10): 1056–1062.
  • Kaya S, Ögün M, Özen H, Kuru M, Sahin L, Kükürt A, Kaçar C. The impact of endometritis on specific oxidative stress parameters in cows. J. Hell. Vet. Med. Soc. 2017;68(2): 231–236.
  • Kim IH, Na KJ, Yang MP. Immune responses during the peripartum period in dairy cows with postpartum endometritis. J. Reprod. Dev. 2005;51(6): 757–764.
  • Küplülü Ş, Vural R, POLAT İM. İneklerde Uterus Enfeksiyonlarının Etiyopatogenezisi, Klinik Semptomları ve Sağaltım Girişimleri. Turkiye Klin. J. Vet. Sci. 2011;2(1): 58–65.
  • Kyo E, Uda N, Suzuki A, Kakimoto M, Ushijima M, Kasuga S, Itakura Y. Immunomodulation and antitumor activities of aged garlic extract. Phytomedicine. 1998;5(4): 259–267.
  • Ley WB, Bowen JM, Sponenberg DP, Lessard PN. Dimethyl sulfoxide intrauterine therapy in the mare: Effects upon endometrial histological features and biopsy classification. Theriogenology. Elsevier; 1989;32(2): 263–276.
  • Li DJ, Liu YF, Li YF, Lv Y, Pei XY, Guo DZ. Significance of nitric oxide concentration in plasma and uterine secretes with puerperal endometritis in dairy cows. Vet. Res. Commun. 2010;34(4): 315–321.
  • Liu IKM. The diagnosis, causes and treatment of persistent endometritis in the mare Rev. Bras. Reprod. Anim. 2011;35(2): 256-261.
  • Loncar KD, Ferris RA, McCue PM, Borlee GI, Hennet ML, Borlee BR. In Vitro Biofilm Disruption and Bacterial Killing Using Nonantibiotic Compounds Against Gram-Negative Equine Uterine Pathogens. J. Equine Vet. Sci. W.B. Saunders; 2017;53: 94–99.
  • Murat N, Demir Ö, Can E, Gidener S, Esen A. Optimum concentration of DMSO in human corpus cavernosum studies. Turk Urol. Derg. 2005;31(1): 17-20.
  • Noakes DE, Parkinson TJ, England GCW. Arthur’s Veterinary Reproduction and Obstetrics - E-Book - David E. Noakes, Timothy J. Parkinson, Gary C. W. England - 8th ed. Sounders W, editor. 2001. London
  • Oral H, Kuru M, Kulaksız R, Kaya S. Kronik endometritisli ineklerde intrauterin uygulanan kekik yağının gebe kalma oranı üzerine etkisi. Lalahan Hayvancılık Araştırma Enstitüsü Derg. 2014;54(2): 57–61.
  • Pande M, Das GK, Khan FA, Sarkar M, Pathak MC, Prasad JK, Kumar H. Endometritis impairs luteal development, function, and nitric oxide and ascorbic acid concentrations in buffalo (Bubalus bubalis). Trop. Anim. Health Prod. 2013;45(3): 805–810.
  • Pinedo PJ, Velez JS, Bothe H, Merchan D, Piñeiro JM, Risco CA. Effect of intrauterine infusion of an organic-certified product on uterine health, survival, and fertility of dairy cows with toxic puerperal metritis. J. Dairy Sci. 2015;98(5): 3120–3132.
  • Sarkar P, Kumar H, Rawat M, Varshney VP, Goswami TK, Yadav MC, Srivastava SK. Effect of administration of garlic extract and PGF2α on hormonal changes and recovery in endometritis cows. Asian-Australasian J. Anim. Sci. 2006;19(7): 964–969.
  • Tsousis G, Sharifi AR, Hoedemaker M. Increased risk of conception failure in German holstein friesian cows with chronic endometritis. Reprod. Domest. Anim. 2010;45(6): 1114–1117.
  • Xue S, De-jun L, Guo-feng F, Bei L, Yun-feng L. Dynamic Analysis of Nitric Oxide and Total Oxidant Capacity in Cow Uterine Secretion with Subclinical Endometritis. J. Northeast Agric. Univ. (English Ed. Elsevier BV; 2015;22(1): 35–39.
  • Yanishlieva N V., Marinova E, Pokorný J. Natural antioxidants from herbs and spices. Eur. J. Lipid Sci. Technol. 2006;108: 776–793.
  • Yaralioglu-Gurgoze S, Cetin H, Cen O, Yilmaz S, Atli MO. Changes in malondialdehyde concentrations and glutathione peroxidase activity in purebred Arabian mares with endometritis. Vet. J. W.B. Saunders; 2005;170(1): 135–137.
  • Yoshida H, Iwata N, Katsuzaki H, Naganawa R, Ishikawa K, Fukuda H, Fujino T, Suzuki A. Antimicrobial activity of a compound isolated from an oil-macerated garlic extract. Biosci. Biotechnol. Biochem. 1998;62(5): 1014–1017.

The Effect of Intrauterine Thyme Essential Oil and Dimethyl Sulfoxide Infusion on Clinical Recovery and Serum Haptoglobin, Tumor Necrosis Factor and Nitric Oxide Levels in Cows with Clinical Endometritis

Year 2021, Volume: 14 Issue: 1, 45 - 50, 31.03.2021
https://doi.org/10.30607/kvj.772613

Abstract

This study, it was aimed to determine the effect of the intrauterine infusion of thyme essential oil and dimethyl sulphoxide (DMSO) on serum haptoglobin (Hp), tumor necrosis factor (TNF) and nitric oxide (NO) levels and on healing in cows with clinical endometritis. In the study, 90 cows with clinical endometritis were randomly assigned to three groups. Cows in the first group (GI, n=30) were treated with an intrauterine infusion of the 120 mL mixture prepared from 20 mL thyme essential oil, 12 mL of DMSO and 88 mL of distilled water. In the second group of cows (GII; n = 30), 20 mL of thyme essential oil was added into 100 mL of distilled water and given intrauterine. The cows in the last group (GIII; n=30) were given intrauterine after mixing 12 mL of DMSO with 108 mL of distilled water. Rectal, ultrasonographic and vaginoscopic examination were performed 14 days after the treatment. Blood was collected from all animals immediately before and 14 days after treatment. Hp, TNF and NO levels in blood serums were determined by the ELISA commercial kits method. Hp, TNF and NO levels decreased significantly after treatment in all groups. The clinical recovery rate was found to be at the highest GI (93.3%). It was determined as 73.3% in GII and 56.6% in GIII. As a result, it was determined that DMSO administration intrauterine together with thyme essential oil treatment contributed positively to the clinical improvement of endometritis.

Project Number

2016-TS-47

References

  • Ahmadi MR, Derakhshandeh A, Shirian S, Daneshbod Y, Ansari-Lari M, Nazifi S. Detection of bacterial biofilm in uterine of repeat breeder dairy cows. Asian Pac. J. Reprod. 2017;6(3): 136-139.
  • Biswal SS, Das S, Balasubramanian S, Mohanty DN, Sethy K, Dasgupta M. Serum amyloid A and haptoglobin levels in crossbred cows with endometritis following different therapy. Vet. World. 2014;7(12): 1066–1070.
  • Çetin B, Çakmakçi S, Çakmakçi R. The investigation of antimicrobial activity of thyme and oregano essential oils. Turkish J. Agric. For. 2011;35(2): 145–154.
  • Chan JPW, Chu CC, Fung HP, Chuang S Te, Lin YC, Chu RM, Lee SL. Serum haptoglobin concentration in cattle. J. Vet. Med. Sci. 2004;66(1): 43–46.
  • Dubuc J, Duffield TF, Leslie KE, Walton JS, LeBlanc SJ. Randomized clinical trial of antibiotic and prostaglandin treatments for uterine health and reproductive performance in dairy cows. J. Dairy Sci. Elsevier; 2011;94(3): 1325–1338.
  • Erçetin E, Çetin Y. Effect of postpartum routıne ıntrauterıne treatment on fertılıty ın daıry cows. Van Vet. J. [Internet]. Van Veterinary Journal; 2020;(2): 60–66.
  • Fischer C, Drillich M, Odau S, Heuwieser W, Einspanier R, Gabler C. Selected pro-inflammatory factor transcripts in bovine endometrial epithelial cells are regulated during the oestrous cycle and elevated in case of subclinical or clinical endometritis. Reprod. Fertil. Dev. 2010;22(5): 818–829.
  • Gilbert RO. The effects of endometritis on the establishment of pregnancy in cattle. Reprod. Fertil. Dev. 2012;24(1): 252–257.
  • Hirvonen J, Huszenicza G, Kulcsàr M, Pyörälä S. Acute-phase response in dairy cows with acute postpartum metritis. Theriogenology. Elsevier Inc. 1999;51(6): 1071–1083.
  • Huzzey JM, Duffield TF, LeBlanc SJ, Veira DM, Weary DM, Von Keyserlingk MAG. Short communication: Haptoglobin as an early indicator of metritis. J. Dairy Sci. American Dairy Science Association. 2009;92(2): 621–625.
  • Kaçar C, Kaya S. İneklerde uterus enfeksiyonları ve üreme performansı üzerine etkisi. Kafkas Univ. Vet. Fak. Derg. 2014;20(6): 975–982.
  • Kaya S, Merhan O, Kacar C, Colak A, Bozukluhan K. Determination of ceruloplasmin, some other acute phase proteins, and biochemical parameters in cows with endometritis. Vet. World. 2016;9(10): 1056–1062.
  • Kaya S, Ögün M, Özen H, Kuru M, Sahin L, Kükürt A, Kaçar C. The impact of endometritis on specific oxidative stress parameters in cows. J. Hell. Vet. Med. Soc. 2017;68(2): 231–236.
  • Kim IH, Na KJ, Yang MP. Immune responses during the peripartum period in dairy cows with postpartum endometritis. J. Reprod. Dev. 2005;51(6): 757–764.
  • Küplülü Ş, Vural R, POLAT İM. İneklerde Uterus Enfeksiyonlarının Etiyopatogenezisi, Klinik Semptomları ve Sağaltım Girişimleri. Turkiye Klin. J. Vet. Sci. 2011;2(1): 58–65.
  • Kyo E, Uda N, Suzuki A, Kakimoto M, Ushijima M, Kasuga S, Itakura Y. Immunomodulation and antitumor activities of aged garlic extract. Phytomedicine. 1998;5(4): 259–267.
  • Ley WB, Bowen JM, Sponenberg DP, Lessard PN. Dimethyl sulfoxide intrauterine therapy in the mare: Effects upon endometrial histological features and biopsy classification. Theriogenology. Elsevier; 1989;32(2): 263–276.
  • Li DJ, Liu YF, Li YF, Lv Y, Pei XY, Guo DZ. Significance of nitric oxide concentration in plasma and uterine secretes with puerperal endometritis in dairy cows. Vet. Res. Commun. 2010;34(4): 315–321.
  • Liu IKM. The diagnosis, causes and treatment of persistent endometritis in the mare Rev. Bras. Reprod. Anim. 2011;35(2): 256-261.
  • Loncar KD, Ferris RA, McCue PM, Borlee GI, Hennet ML, Borlee BR. In Vitro Biofilm Disruption and Bacterial Killing Using Nonantibiotic Compounds Against Gram-Negative Equine Uterine Pathogens. J. Equine Vet. Sci. W.B. Saunders; 2017;53: 94–99.
  • Murat N, Demir Ö, Can E, Gidener S, Esen A. Optimum concentration of DMSO in human corpus cavernosum studies. Turk Urol. Derg. 2005;31(1): 17-20.
  • Noakes DE, Parkinson TJ, England GCW. Arthur’s Veterinary Reproduction and Obstetrics - E-Book - David E. Noakes, Timothy J. Parkinson, Gary C. W. England - 8th ed. Sounders W, editor. 2001. London
  • Oral H, Kuru M, Kulaksız R, Kaya S. Kronik endometritisli ineklerde intrauterin uygulanan kekik yağının gebe kalma oranı üzerine etkisi. Lalahan Hayvancılık Araştırma Enstitüsü Derg. 2014;54(2): 57–61.
  • Pande M, Das GK, Khan FA, Sarkar M, Pathak MC, Prasad JK, Kumar H. Endometritis impairs luteal development, function, and nitric oxide and ascorbic acid concentrations in buffalo (Bubalus bubalis). Trop. Anim. Health Prod. 2013;45(3): 805–810.
  • Pinedo PJ, Velez JS, Bothe H, Merchan D, Piñeiro JM, Risco CA. Effect of intrauterine infusion of an organic-certified product on uterine health, survival, and fertility of dairy cows with toxic puerperal metritis. J. Dairy Sci. 2015;98(5): 3120–3132.
  • Sarkar P, Kumar H, Rawat M, Varshney VP, Goswami TK, Yadav MC, Srivastava SK. Effect of administration of garlic extract and PGF2α on hormonal changes and recovery in endometritis cows. Asian-Australasian J. Anim. Sci. 2006;19(7): 964–969.
  • Tsousis G, Sharifi AR, Hoedemaker M. Increased risk of conception failure in German holstein friesian cows with chronic endometritis. Reprod. Domest. Anim. 2010;45(6): 1114–1117.
  • Xue S, De-jun L, Guo-feng F, Bei L, Yun-feng L. Dynamic Analysis of Nitric Oxide and Total Oxidant Capacity in Cow Uterine Secretion with Subclinical Endometritis. J. Northeast Agric. Univ. (English Ed. Elsevier BV; 2015;22(1): 35–39.
  • Yanishlieva N V., Marinova E, Pokorný J. Natural antioxidants from herbs and spices. Eur. J. Lipid Sci. Technol. 2006;108: 776–793.
  • Yaralioglu-Gurgoze S, Cetin H, Cen O, Yilmaz S, Atli MO. Changes in malondialdehyde concentrations and glutathione peroxidase activity in purebred Arabian mares with endometritis. Vet. J. W.B. Saunders; 2005;170(1): 135–137.
  • Yoshida H, Iwata N, Katsuzaki H, Naganawa R, Ishikawa K, Fukuda H, Fujino T, Suzuki A. Antimicrobial activity of a compound isolated from an oil-macerated garlic extract. Biosci. Biotechnol. Biochem. 1998;62(5): 1014–1017.
There are 31 citations in total.

Details

Primary Language Turkish
Subjects Veterinary Sciences
Journal Section RESEARCH ARTICLE
Authors

Semra Kaya 0000-0002-7520-6631

Cihan Kaçar 0000-0002-2642-697X

Oğuz Merhan 0000-0002-3399-0667

Murat Can Demir 0000-0002-3803-069X

Umut Çağın Arı 0000-0002-7089-6522

Abuzer K. Zonturlu 0000-0002-9537-5117

Project Number 2016-TS-47
Publication Date March 31, 2021
Acceptance Date December 17, 2020
Published in Issue Year 2021 Volume: 14 Issue: 1

Cite

APA Kaya, S., Kaçar, C., Merhan, O., Demir, M. C., et al. (2021). Klinik Endometritisli İneklerde İntrauterin Kekik Esansiyel Yağı ile Birlikte Dimetil Sülfoksit Uygulamasının Klinik İyileşme Üzerine ve Serum Haptoglobin, Tümör Nekrozis Faktör ve Nitrik Oksit Düzeylerine Etkisi. Kocatepe Veterinary Journal, 14(1), 45-50. https://doi.org/10.30607/kvj.772613
AMA Kaya S, Kaçar C, Merhan O, Demir MC, Arı UÇ, Zonturlu AK. Klinik Endometritisli İneklerde İntrauterin Kekik Esansiyel Yağı ile Birlikte Dimetil Sülfoksit Uygulamasının Klinik İyileşme Üzerine ve Serum Haptoglobin, Tümör Nekrozis Faktör ve Nitrik Oksit Düzeylerine Etkisi. kvj. March 2021;14(1):45-50. doi:10.30607/kvj.772613
Chicago Kaya, Semra, Cihan Kaçar, Oğuz Merhan, Murat Can Demir, Umut Çağın Arı, and Abuzer K. Zonturlu. “Klinik Endometritisli İneklerde İntrauterin Kekik Esansiyel Yağı Ile Birlikte Dimetil Sülfoksit Uygulamasının Klinik İyileşme Üzerine Ve Serum Haptoglobin, Tümör Nekrozis Faktör Ve Nitrik Oksit Düzeylerine Etkisi”. Kocatepe Veterinary Journal 14, no. 1 (March 2021): 45-50. https://doi.org/10.30607/kvj.772613.
EndNote Kaya S, Kaçar C, Merhan O, Demir MC, Arı UÇ, Zonturlu AK (March 1, 2021) Klinik Endometritisli İneklerde İntrauterin Kekik Esansiyel Yağı ile Birlikte Dimetil Sülfoksit Uygulamasının Klinik İyileşme Üzerine ve Serum Haptoglobin, Tümör Nekrozis Faktör ve Nitrik Oksit Düzeylerine Etkisi. Kocatepe Veterinary Journal 14 1 45–50.
IEEE S. Kaya, C. Kaçar, O. Merhan, M. C. Demir, U. Ç. Arı, and A. K. Zonturlu, “Klinik Endometritisli İneklerde İntrauterin Kekik Esansiyel Yağı ile Birlikte Dimetil Sülfoksit Uygulamasının Klinik İyileşme Üzerine ve Serum Haptoglobin, Tümör Nekrozis Faktör ve Nitrik Oksit Düzeylerine Etkisi”, kvj, vol. 14, no. 1, pp. 45–50, 2021, doi: 10.30607/kvj.772613.
ISNAD Kaya, Semra et al. “Klinik Endometritisli İneklerde İntrauterin Kekik Esansiyel Yağı Ile Birlikte Dimetil Sülfoksit Uygulamasının Klinik İyileşme Üzerine Ve Serum Haptoglobin, Tümör Nekrozis Faktör Ve Nitrik Oksit Düzeylerine Etkisi”. Kocatepe Veterinary Journal 14/1 (March 2021), 45-50. https://doi.org/10.30607/kvj.772613.
JAMA Kaya S, Kaçar C, Merhan O, Demir MC, Arı UÇ, Zonturlu AK. Klinik Endometritisli İneklerde İntrauterin Kekik Esansiyel Yağı ile Birlikte Dimetil Sülfoksit Uygulamasının Klinik İyileşme Üzerine ve Serum Haptoglobin, Tümör Nekrozis Faktör ve Nitrik Oksit Düzeylerine Etkisi. kvj. 2021;14:45–50.
MLA Kaya, Semra et al. “Klinik Endometritisli İneklerde İntrauterin Kekik Esansiyel Yağı Ile Birlikte Dimetil Sülfoksit Uygulamasının Klinik İyileşme Üzerine Ve Serum Haptoglobin, Tümör Nekrozis Faktör Ve Nitrik Oksit Düzeylerine Etkisi”. Kocatepe Veterinary Journal, vol. 14, no. 1, 2021, pp. 45-50, doi:10.30607/kvj.772613.
Vancouver Kaya S, Kaçar C, Merhan O, Demir MC, Arı UÇ, Zonturlu AK. Klinik Endometritisli İneklerde İntrauterin Kekik Esansiyel Yağı ile Birlikte Dimetil Sülfoksit Uygulamasının Klinik İyileşme Üzerine ve Serum Haptoglobin, Tümör Nekrozis Faktör ve Nitrik Oksit Düzeylerine Etkisi. kvj. 2021;14(1):45-50.

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