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Koyunlarda Plazma Sitokin ve Lipid Peroksidasyon Düzeylerine Yaşın Etkisi

Year 2021, Volume 14, Issue 1, 37 - 44, 31.03.2021
https://doi.org/10.30607/kvj.798623

Abstract

Bu çalışmanın amacı, değişik yaşlardaki koyunlarda kan sitokin düzeyleri ve lipid peroksidasyon ürünü malondialdehid (MDA) seviyesi ile tiroid hormonları düzeylerine yaşın etkilerinin belirlenmesidir. Çalışmada, aynı bakım ve besleme şartlarındaki bir sürüden kuzu (4-6 aylık, kuzu), genç (12-18 aylık) ve ergin (36-40 aylık) dişi koyun kullanıldı. Her bir yaş grubundan 10 baş olmak üzere toplam 30 koyundan sabah yemlemesinden önce kan örnekleri alındı. Kan örneklerinde MDA, glutatyon (GSH), interlökin-1 beta (IL-1β), tümör nekrozis faktör alfa (TNF-α) ve interlökin-6 (IL-6) ile T3 ve T4 düzeyleri belirlendi. Genç koyunların kuzularınkinden önemli oranda daha yüksek plazma MDA ve daha düşük IL-6 düzeylerine sahip oldukları bulundu. GSH, IL-1β, TNF-α, T3 ve T4 düzeyleri bakımından yaş grupları arasında farkın olmadığı saptandı. Sonuç olarak, koyunlarda kan lipid peroksidasyonu ve IL-6 düzeylerine yaşın etkisi olduğu söylenebilir.

References

  • Akçapınar H. Koyun Yetiştiriciliği. MEDİSAN Yayınevi. 1994.1.Baskı. Ankara.
  • Antunović Z., Novoselec J., Sauerwein H., Speranda M., Vegara M., Pavic V. Blood metabolic profile and some of hormones concentration in ewes during different physiological status. Bulg. J.Agric. Sci., 2011; 17: 687-695.
  • Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione. J. Lab. Clin. Med., 1963; 61: 882-888.
  • Burton AB, Wagner B, Erb HN., Ainsworth DM. Serum interleukin-6 and IL-10 concentrations in normal and septic foals. Vet Immunol Immunopathol, 2009; 132: 122-128.
  • Conner E.M., Grisham M.B. 1996. Inflammation, free radicals, and antioxidants. Nutrition., 12(4):274–277.
  • Craig N.M., Smith D.W., Pate J.A., Morrison I.W., Knight P.A. Local cytokine transcription in naïve and previously infected sheep and lambs following challenge with Teladorsagia circumcincta. BMC Veterinary Research 2014; 10:87.
  • Dhama K., Mahendran M., Chauhan R.S., Tomar S. Cytokines: their functional roles and prospective applications in veterinary practice: A review. J. Immunol. Immunopathol. 2008; 10:79-89.
  • Dobson RJ., Waller PJ., Donald AD. Population dynamics of Trichostrongylus colubriformis in sheep: the effect of host age on the establishment of infective larvae. Int J Parasitol, 1990; 20:353–357.
  • Draper HH., Hardley M. Malondialdehyde determination as index of lipid peroxidation. Methods in Enzymology, 1990; 186: 421-431.
  • Dröge W., Breitkreutz R. Glutathione and immune function.Proc.Nutr.Soc., 2000; 59:595-600.
  • Elmarakby A.A., Sullivan J.C. Relationship between oxidative stress and inflammatory cytokines in diabetic nephropathy. Cardiovascular Therapeutics, 2012; 30: 49–59.
  • Elsabahy M., Wooley K.L. Cytokines as biomarkers of nanoparticle immunotoxicity, Chem. Soc. Rev. 2013; 42: 5552–5576.
  • Eryavuz A., Küçükkurt İ., İnce S., Fidan AF., Avcı G., Bülbül T. Kuzularda Rasyona Yucca Schidigera Tozu Katılması ve Günlük Dozunun Rumen Fermentasyonu ile Verime Etkilerinin Araştırılması. Kocatepe V.J., 2015; 8:1-10.
  • Fernández A., Marteles D., Ruiz de Arcaute M., Lacasta D., Conde T., Loste A. Relationship between Pro-Inflammatory Cytokines, IL-10 Anti-Inflammatory Cytokine and Serum Proteins in Healthy Lambs and with Diarrhea. Pakistan Vet.J., 2016; 36(1): 63-67.
  • Foster D.L., Hileman S.L. Puberty in the sheep. Knobil and Neill's Physiology of Reproduction (Fourth Edition), 2015; pp, 1441-1485.
  • Fulop T., Larbi A., Dupuis G., Le Page A., Frost E.H., Cohen A.A., Witkowski J.M., Franceschi C. Immunosenescence and Inflamm-Aging As Two Sides of the Same Coin: Friends or Foes? Frontiers İmmul., 2018; 8:1960.
  • Gilgun-Sherki Y., Melamed E., Offen D. Oxidative stress induced neurodegenerative diseases: the need for antioxidants that penetrate the blood brain barrier. Neuropharmacology 2001; 40: 959–975.
  • Huang M.C., Greig N.H., Luo W., Tweedie D., Schwartz J.B., Longo D.L. Preferential enhancement of older human T cell cytokine generation, chemotaxis, proliferation and survival by lenalidomide. Clin. Immunol. 2011; 138 (2):201–211.
  • Huszenicza GY., Kulcsar M., Rudas P. Clinical endocrinology of thyroid gland function in ruminants. Vet. Med. (Czech), . 2002; 47:199–210.
  • John J., Haddad A. Redox microenvironment is essential for MAPK-dependent secretion of pro-inflammatory cytokines: Modulation by glutathione (GSH/GSSG) biosynthesis and equilibrium in the alveolar epithelium, Cellular Immunology, 2011; 270: 53-61.
  • Kandemir Ç., Adanacıoğlu H., Taşkın T., Koşum N. Türkiye’de Koyun ve Koyun Eti Fiyatlarının Bölgelere Göre Çok Boyutlu Ölçekleme Analizi ile Karşılaştırılması. Tekirdağ Ziraat Fakültesi Dergisi, 2019; 16(3): 315-327.
  • Kandır S., Keskin E. Serum IL-1β, IL-6, IL-10 and TNF-α Levels in Thyroidectomized Rats. Kafkas Univ Vet Fak Derg., 2016; 22: 297-300.
  • Kumar A.A., Tripathi B.N., Sharma B. Cytokine Profile in Tissues and Blood of Sheep Experimentally Infected with Mycobacterium avium subsp. Paratuberculosis. J. Appl. Anim. Res. 2010; 38: 185-189.
  • Lockwood CJ., Yen CF., Basar M., Kayisli UA., Martel M. Buhimschi IPreeclampsia-related inflammatory cytokines regulate interleukin-6 expression in human decidual cells. Am J Pathol., . 2008; 172(6): 1571–1579.
  • Maggio M., Guralnik JM., Longo DL., Ferrucci L. Interleukin-6 in aging and chronic disease: a magnificent pathway. J Gerontol A Biol Sci Med Sci, 2006; 61: 575–584.
  • Maher P. The effects of stress and aging on glutathione metabolism. Ageing Res.Rev., 2005; 4: 288-314.
  • Marchiori R.C., Pereira L.A., Naujorks A.A., Rovaris D.L., Meinerz D.F., Duarte M.M., Rocha J.B. Improvement of blood inflammatory marker levels in patients with hypothyroidism under levothyroxine treatment. BMC Endocr. Disord., 2015; 15:32 DOI 10.1186/s12902-015-0032-3.
  • Neito C.A.R., Ferguson M.B., Macleay C.A., Briegel J.R., Thompson A.N. Selection for superior growth advances the onset of puberty and increases reproductive performance in ewe lambs. Animal, 2013; 7(6): 990-997.
  • Pamir N., McMillen TS., Kaiyala KJ., Schwartz MW., LeBoeuf RC. Receptors for tumor necrosis factor-alpha play a protective role against obesity and alter adipose tissue macrophage status. Endocrinology, 2009; 50: 4124–4134.
  • Pawelec G. Age and immunity: What is “immunosenescence”? Exp.Gerant., 2018; 105: 4-9.
  • Pena L.R., Hill D.B., McClain C.J. Treatment with glutathione precursor decreases cytokine activity. J.Parenter. Enteral.Nutr., 1999; 23: 1-6.
  • Pérez-Navero J.L., Benitez-Sillero J.D., Gil-Campos M., Guillén-del Castillo M., Tasset I., Túnez I. Changes in oxidative stress biomarkers induced by puberty. An. Pediatr (Barc.), 2009; 70: 424-428.
  • Pollak Y., Yirmiya R. Cytokin-induced changes in mood and behaviour: implications for “depression due to a general medical condition”, immunotherapy and antidepressive treatment. Int.J.Neuropsychopharmacol., 2002; 5: 389-399.
  • Scheerlinck J.P.Y., Yen H.H. Veterinary applications of cytokines. Vet Immunol Immunopathol, 2005; 108: 17-22.
  • Sezenler T., Özder M. Türkgeldi Koyunlarında Erken Yaşta Kuzulatmanın Çeşitli Verim Özelliklerine Etkisi. Tekirdağ Ziraat Fak.Derg., 2007; 4 (1): 91-97.
  • Shelly C., Lu M.D. Glutathione Synthesis. Biochim Biophys Acta., 2013; 1830(5): 3143–3153.
  • Smarsh D.N., Williams C.A. Oxidative Stress and Antioxidant Status in Standardbreds: Effect of Age and Acute Exercise Before and After Training. J.Equine Vet.Sci., 2016; 47: 92-106.
  • Stowe R.P., Peek M.K., Cutchin M.P., Goodwin J.S. Plasma Cytokine Levels in a Population-Based Study: Relation to Age and Ethnicity. J. Gerontol. A Biol. Sci. Med. Sci., 2010; 65A (4): 429-433.
  • Suchard M. Immunosenescence: ageing of the immune system. S. Afr. Pharm. J., 2015; 82: 28-31.
  • Suwa T., Hogg JC., English D., van Eeden SF. Interleukin-6 induces demargination of intravascular neutrophils and shortens their transit in marrow. Am J Physiol Heart Circ Physiol 2000; 279: H2954–60.
  • Şeker İ., Köseman A., Baykalır Y., Şeker P. Koyun Karkaslarının Kalite Sınıflandırılmasında “EUROP” Sistemi ve Türkiye’deki Uygulamalar. Iğdır Üni. Fen Bilimleri Enst. Der., 2017; 7(3): 309-320.
  • Takcı A. Modifiye U-synch Protokolü ile Mevsim Dışı Senkronize Edilen Akkaraman Kangal Irkı Prepubertal Kuzu ve Primipar Koyunlara GnRH ve hCG Uygulamalarının Fertilite Üzerine Etkisi. Turk Vet J., . 2019; 1(2) : 60-68.
  • Tayde P., Bhagwat N., Sharma P., Sharma B., Dalwadi P., Sonawane A., Subramanyam A., Chadha M., Varthakavi P. Hypothyroidism and depression: are cytokines the link? Indian J. Endocrinol. Metab., . 2017; 21: 886-892.
  • Thompson A., Bairstow C., Ferguson M., Kearney G., Macleay C., Thompson H., Pagagoni B. Growth pattern to the end of the mating period influences the reproductive performance of merino ewe lambs mated at 7 to 8 months of age. Small Rum.Res., 2019; 179: 1-6.
  • Valasi I., Chadio S., Fthenakis GC., Amiridis GS. Management of pre-pubertal small ruminants: Physiological basis and clinical approach. Anim Reprod Sci 2012; 130(3-4): 126-134
  • Westendorp R.G.J. Are we becoming less disposable? EMBO reports, 2004; 5 (1): 2-6.
  • Yalçın A.D., Terzioğlu E., Gorczynski R.M. İmmün Yaşlanma. Türk Geriatri Derg., 2011; 14: 276-280.
  • Zhao W., Diz D.I., Robbins M.E. Oxidative damage pathways in relation to normal tissue injury. The British Journal of Radiology 2007; 80(1) : 23-31.

Effects of Age on The Concentrations of Plasma Cytokines and Lipidperoxidation in Sheep

Year 2021, Volume 14, Issue 1, 37 - 44, 31.03.2021
https://doi.org/10.30607/kvj.798623

Abstract

The aim of this study is to determine the effects of age on blood cytokine levels and lipid peroxidation product malondialdehyde (MDA) levels and thyroid hormones levels in sheep of different ages. Female sheep of lamb (4-6 months), young (12-18 months) and adult (36-40 months) from a herd in same care and feeding conditions were used in the study. Blood samples were taken before morning feding from 30 sheep in total, 10 heads from each age group. MDA, glutathione (GSH), interleukin-1 (IL-1), tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6) and T3 and T4 levels were determined in blood samples. Young animals have significantly higher MDA and lower IL-6 levels than lambs. There was no difference between age groups in terms of GSH, IL-1β, TNF-α, T3 and T4 levels. As a result, age has an effect on blood lipid peroxidation and IL-6 levels in sheep.

References

  • Akçapınar H. Koyun Yetiştiriciliği. MEDİSAN Yayınevi. 1994.1.Baskı. Ankara.
  • Antunović Z., Novoselec J., Sauerwein H., Speranda M., Vegara M., Pavic V. Blood metabolic profile and some of hormones concentration in ewes during different physiological status. Bulg. J.Agric. Sci., 2011; 17: 687-695.
  • Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione. J. Lab. Clin. Med., 1963; 61: 882-888.
  • Burton AB, Wagner B, Erb HN., Ainsworth DM. Serum interleukin-6 and IL-10 concentrations in normal and septic foals. Vet Immunol Immunopathol, 2009; 132: 122-128.
  • Conner E.M., Grisham M.B. 1996. Inflammation, free radicals, and antioxidants. Nutrition., 12(4):274–277.
  • Craig N.M., Smith D.W., Pate J.A., Morrison I.W., Knight P.A. Local cytokine transcription in naïve and previously infected sheep and lambs following challenge with Teladorsagia circumcincta. BMC Veterinary Research 2014; 10:87.
  • Dhama K., Mahendran M., Chauhan R.S., Tomar S. Cytokines: their functional roles and prospective applications in veterinary practice: A review. J. Immunol. Immunopathol. 2008; 10:79-89.
  • Dobson RJ., Waller PJ., Donald AD. Population dynamics of Trichostrongylus colubriformis in sheep: the effect of host age on the establishment of infective larvae. Int J Parasitol, 1990; 20:353–357.
  • Draper HH., Hardley M. Malondialdehyde determination as index of lipid peroxidation. Methods in Enzymology, 1990; 186: 421-431.
  • Dröge W., Breitkreutz R. Glutathione and immune function.Proc.Nutr.Soc., 2000; 59:595-600.
  • Elmarakby A.A., Sullivan J.C. Relationship between oxidative stress and inflammatory cytokines in diabetic nephropathy. Cardiovascular Therapeutics, 2012; 30: 49–59.
  • Elsabahy M., Wooley K.L. Cytokines as biomarkers of nanoparticle immunotoxicity, Chem. Soc. Rev. 2013; 42: 5552–5576.
  • Eryavuz A., Küçükkurt İ., İnce S., Fidan AF., Avcı G., Bülbül T. Kuzularda Rasyona Yucca Schidigera Tozu Katılması ve Günlük Dozunun Rumen Fermentasyonu ile Verime Etkilerinin Araştırılması. Kocatepe V.J., 2015; 8:1-10.
  • Fernández A., Marteles D., Ruiz de Arcaute M., Lacasta D., Conde T., Loste A. Relationship between Pro-Inflammatory Cytokines, IL-10 Anti-Inflammatory Cytokine and Serum Proteins in Healthy Lambs and with Diarrhea. Pakistan Vet.J., 2016; 36(1): 63-67.
  • Foster D.L., Hileman S.L. Puberty in the sheep. Knobil and Neill's Physiology of Reproduction (Fourth Edition), 2015; pp, 1441-1485.
  • Fulop T., Larbi A., Dupuis G., Le Page A., Frost E.H., Cohen A.A., Witkowski J.M., Franceschi C. Immunosenescence and Inflamm-Aging As Two Sides of the Same Coin: Friends or Foes? Frontiers İmmul., 2018; 8:1960.
  • Gilgun-Sherki Y., Melamed E., Offen D. Oxidative stress induced neurodegenerative diseases: the need for antioxidants that penetrate the blood brain barrier. Neuropharmacology 2001; 40: 959–975.
  • Huang M.C., Greig N.H., Luo W., Tweedie D., Schwartz J.B., Longo D.L. Preferential enhancement of older human T cell cytokine generation, chemotaxis, proliferation and survival by lenalidomide. Clin. Immunol. 2011; 138 (2):201–211.
  • Huszenicza GY., Kulcsar M., Rudas P. Clinical endocrinology of thyroid gland function in ruminants. Vet. Med. (Czech), . 2002; 47:199–210.
  • John J., Haddad A. Redox microenvironment is essential for MAPK-dependent secretion of pro-inflammatory cytokines: Modulation by glutathione (GSH/GSSG) biosynthesis and equilibrium in the alveolar epithelium, Cellular Immunology, 2011; 270: 53-61.
  • Kandemir Ç., Adanacıoğlu H., Taşkın T., Koşum N. Türkiye’de Koyun ve Koyun Eti Fiyatlarının Bölgelere Göre Çok Boyutlu Ölçekleme Analizi ile Karşılaştırılması. Tekirdağ Ziraat Fakültesi Dergisi, 2019; 16(3): 315-327.
  • Kandır S., Keskin E. Serum IL-1β, IL-6, IL-10 and TNF-α Levels in Thyroidectomized Rats. Kafkas Univ Vet Fak Derg., 2016; 22: 297-300.
  • Kumar A.A., Tripathi B.N., Sharma B. Cytokine Profile in Tissues and Blood of Sheep Experimentally Infected with Mycobacterium avium subsp. Paratuberculosis. J. Appl. Anim. Res. 2010; 38: 185-189.
  • Lockwood CJ., Yen CF., Basar M., Kayisli UA., Martel M. Buhimschi IPreeclampsia-related inflammatory cytokines regulate interleukin-6 expression in human decidual cells. Am J Pathol., . 2008; 172(6): 1571–1579.
  • Maggio M., Guralnik JM., Longo DL., Ferrucci L. Interleukin-6 in aging and chronic disease: a magnificent pathway. J Gerontol A Biol Sci Med Sci, 2006; 61: 575–584.
  • Maher P. The effects of stress and aging on glutathione metabolism. Ageing Res.Rev., 2005; 4: 288-314.
  • Marchiori R.C., Pereira L.A., Naujorks A.A., Rovaris D.L., Meinerz D.F., Duarte M.M., Rocha J.B. Improvement of blood inflammatory marker levels in patients with hypothyroidism under levothyroxine treatment. BMC Endocr. Disord., 2015; 15:32 DOI 10.1186/s12902-015-0032-3.
  • Neito C.A.R., Ferguson M.B., Macleay C.A., Briegel J.R., Thompson A.N. Selection for superior growth advances the onset of puberty and increases reproductive performance in ewe lambs. Animal, 2013; 7(6): 990-997.
  • Pamir N., McMillen TS., Kaiyala KJ., Schwartz MW., LeBoeuf RC. Receptors for tumor necrosis factor-alpha play a protective role against obesity and alter adipose tissue macrophage status. Endocrinology, 2009; 50: 4124–4134.
  • Pawelec G. Age and immunity: What is “immunosenescence”? Exp.Gerant., 2018; 105: 4-9.
  • Pena L.R., Hill D.B., McClain C.J. Treatment with glutathione precursor decreases cytokine activity. J.Parenter. Enteral.Nutr., 1999; 23: 1-6.
  • Pérez-Navero J.L., Benitez-Sillero J.D., Gil-Campos M., Guillén-del Castillo M., Tasset I., Túnez I. Changes in oxidative stress biomarkers induced by puberty. An. Pediatr (Barc.), 2009; 70: 424-428.
  • Pollak Y., Yirmiya R. Cytokin-induced changes in mood and behaviour: implications for “depression due to a general medical condition”, immunotherapy and antidepressive treatment. Int.J.Neuropsychopharmacol., 2002; 5: 389-399.
  • Scheerlinck J.P.Y., Yen H.H. Veterinary applications of cytokines. Vet Immunol Immunopathol, 2005; 108: 17-22.
  • Sezenler T., Özder M. Türkgeldi Koyunlarında Erken Yaşta Kuzulatmanın Çeşitli Verim Özelliklerine Etkisi. Tekirdağ Ziraat Fak.Derg., 2007; 4 (1): 91-97.
  • Shelly C., Lu M.D. Glutathione Synthesis. Biochim Biophys Acta., 2013; 1830(5): 3143–3153.
  • Smarsh D.N., Williams C.A. Oxidative Stress and Antioxidant Status in Standardbreds: Effect of Age and Acute Exercise Before and After Training. J.Equine Vet.Sci., 2016; 47: 92-106.
  • Stowe R.P., Peek M.K., Cutchin M.P., Goodwin J.S. Plasma Cytokine Levels in a Population-Based Study: Relation to Age and Ethnicity. J. Gerontol. A Biol. Sci. Med. Sci., 2010; 65A (4): 429-433.
  • Suchard M. Immunosenescence: ageing of the immune system. S. Afr. Pharm. J., 2015; 82: 28-31.
  • Suwa T., Hogg JC., English D., van Eeden SF. Interleukin-6 induces demargination of intravascular neutrophils and shortens their transit in marrow. Am J Physiol Heart Circ Physiol 2000; 279: H2954–60.
  • Şeker İ., Köseman A., Baykalır Y., Şeker P. Koyun Karkaslarının Kalite Sınıflandırılmasında “EUROP” Sistemi ve Türkiye’deki Uygulamalar. Iğdır Üni. Fen Bilimleri Enst. Der., 2017; 7(3): 309-320.
  • Takcı A. Modifiye U-synch Protokolü ile Mevsim Dışı Senkronize Edilen Akkaraman Kangal Irkı Prepubertal Kuzu ve Primipar Koyunlara GnRH ve hCG Uygulamalarının Fertilite Üzerine Etkisi. Turk Vet J., . 2019; 1(2) : 60-68.
  • Tayde P., Bhagwat N., Sharma P., Sharma B., Dalwadi P., Sonawane A., Subramanyam A., Chadha M., Varthakavi P. Hypothyroidism and depression: are cytokines the link? Indian J. Endocrinol. Metab., . 2017; 21: 886-892.
  • Thompson A., Bairstow C., Ferguson M., Kearney G., Macleay C., Thompson H., Pagagoni B. Growth pattern to the end of the mating period influences the reproductive performance of merino ewe lambs mated at 7 to 8 months of age. Small Rum.Res., 2019; 179: 1-6.
  • Valasi I., Chadio S., Fthenakis GC., Amiridis GS. Management of pre-pubertal small ruminants: Physiological basis and clinical approach. Anim Reprod Sci 2012; 130(3-4): 126-134
  • Westendorp R.G.J. Are we becoming less disposable? EMBO reports, 2004; 5 (1): 2-6.
  • Yalçın A.D., Terzioğlu E., Gorczynski R.M. İmmün Yaşlanma. Türk Geriatri Derg., 2011; 14: 276-280.
  • Zhao W., Diz D.I., Robbins M.E. Oxidative damage pathways in relation to normal tissue injury. The British Journal of Radiology 2007; 80(1) : 23-31.

Details

Primary Language Turkish
Subjects Veterinary Sciences
Journal Section RESEARCH ARTICLE
Authors

Mehmet SALİM This is me
Afyon Kocatepe Üniversitesi Veteriner Fakültesi, Eğitim, Uygulama ve Araştırma Çiftliği
0000-0002-4805-576X
Türkiye


İbrahim DURMUŞ
Afyon Kocatepe Üniversitesi, Şuhut Melek Yüksek Okulu
0000-0003-1360-8843
Türkiye


Mehmet BAŞEĞMEZ
Pamukkale Üniversitesi Acıpayam Meslek Yüksek Okulu
0000-0002-9994-1251
Türkiye


İsmail KÜÇÜKKURT
Afyon Kocatepe Üniversitesi, Veteriner Fakültesi, Biyokimya Anabilim Dalı
0000-0003-0198-629X
Türkiye


Abdullah ERYAVUZ (Primary Author)
Afyon Kocatepe Üniversitesi, Veteriner Fakültesi, Fizyoloji Anabilim Dalı
0000-0001-8602-2400
Türkiye

Supporting Institution Afyon Kocatepe Üniversitesi Bilimsel Araştırmaları Koordinasyon Birimi tarafından desteklenmiştir
Project Number Proje kodu: 18 Kariyer 280
Publication Date March 31, 2021
Published in Issue Year 2021, Volume 14, Issue 1

Cite

Bibtex @research article { kvj798623, journal = {Kocatepe Veterinary Journal}, issn = {1308-1594}, eissn = {2147-6853}, address = {}, publisher = {Afyon Kocatepe University}, year = {2021}, volume = {14}, pages = {37 - 44}, doi = {10.30607/kvj.798623}, title = {Koyunlarda Plazma Sitokin ve Lipid Peroksidasyon Düzeylerine Yaşın Etkisi}, key = {cite}, author = {Salim, Mehmet and Durmuş, İbrahim and Başeğmez, Mehmet and Küçükkurt, İsmail and Eryavuz, Abdullah} }
APA Salim, M. , Durmuş, İ. , Başeğmez, M. , Küçükkurt, İ. & Eryavuz, A. (2021). Koyunlarda Plazma Sitokin ve Lipid Peroksidasyon Düzeylerine Yaşın Etkisi . Kocatepe Veterinary Journal , 14 (1) , 37-44 . DOI: 10.30607/kvj.798623
MLA Salim, M. , Durmuş, İ. , Başeğmez, M. , Küçükkurt, İ. , Eryavuz, A. "Koyunlarda Plazma Sitokin ve Lipid Peroksidasyon Düzeylerine Yaşın Etkisi" . Kocatepe Veterinary Journal 14 (2021 ): 37-44 <https://dergipark.org.tr/en/pub/kvj/issue/58090/798623>
Chicago Salim, M. , Durmuş, İ. , Başeğmez, M. , Küçükkurt, İ. , Eryavuz, A. "Koyunlarda Plazma Sitokin ve Lipid Peroksidasyon Düzeylerine Yaşın Etkisi". Kocatepe Veterinary Journal 14 (2021 ): 37-44
RIS TY - JOUR T1 - Koyunlarda Plazma Sitokin ve Lipid Peroksidasyon Düzeylerine Yaşın Etkisi AU - Mehmet Salim , İbrahim Durmuş , Mehmet Başeğmez , İsmail Küçükkurt , Abdullah Eryavuz Y1 - 2021 PY - 2021 N1 - doi: 10.30607/kvj.798623 DO - 10.30607/kvj.798623 T2 - Kocatepe Veterinary Journal JF - Journal JO - JOR SP - 37 EP - 44 VL - 14 IS - 1 SN - 1308-1594-2147-6853 M3 - doi: 10.30607/kvj.798623 UR - https://doi.org/10.30607/kvj.798623 Y2 - 2021 ER -
EndNote %0 Kocatepe Veterinary Journal Koyunlarda Plazma Sitokin ve Lipid Peroksidasyon Düzeylerine Yaşın Etkisi %A Mehmet Salim , İbrahim Durmuş , Mehmet Başeğmez , İsmail Küçükkurt , Abdullah Eryavuz %T Koyunlarda Plazma Sitokin ve Lipid Peroksidasyon Düzeylerine Yaşın Etkisi %D 2021 %J Kocatepe Veterinary Journal %P 1308-1594-2147-6853 %V 14 %N 1 %R doi: 10.30607/kvj.798623 %U 10.30607/kvj.798623
ISNAD Salim, Mehmet , Durmuş, İbrahim , Başeğmez, Mehmet , Küçükkurt, İsmail , Eryavuz, Abdullah . "Koyunlarda Plazma Sitokin ve Lipid Peroksidasyon Düzeylerine Yaşın Etkisi". Kocatepe Veterinary Journal 14 / 1 (March 2021): 37-44 . https://doi.org/10.30607/kvj.798623
AMA Salim M. , Durmuş İ. , Başeğmez M. , Küçükkurt İ. , Eryavuz A. Koyunlarda Plazma Sitokin ve Lipid Peroksidasyon Düzeylerine Yaşın Etkisi. Kocatepe Veterinary Journal. 2021; 14(1): 37-44.
Vancouver Salim M. , Durmuş İ. , Başeğmez M. , Küçükkurt İ. , Eryavuz A. Koyunlarda Plazma Sitokin ve Lipid Peroksidasyon Düzeylerine Yaşın Etkisi. Kocatepe Veterinary Journal. 2021; 14(1): 37-44.
IEEE M. Salim , İ. Durmuş , M. Başeğmez , İ. Küçükkurt and A. Eryavuz , "Koyunlarda Plazma Sitokin ve Lipid Peroksidasyon Düzeylerine Yaşın Etkisi", Kocatepe Veterinary Journal, vol. 14, no. 1, pp. 37-44, Mar. 2021, doi:10.30607/kvj.798623

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