Kütahya İlindeki Mezbahalarda Kesilen Sığırlarda Pasteurella Türlerinin İzolasyonu, İdentifikasyonu ve Antibiyotik Duyarlılıkları

Year 2023, Volume: 16 Issue: 2, 127 - 130, 30.06.2023

Seydi Mehmet Arslan , Beytullah Kenar

https://doi.org/10.30607/kvj.1169683

Abstract

Yapılan bu çalışmada sığır pnömonilerine sebep olan en önemli bakteriyel etkenlerden biri olan Pasteurella türlerini izole ve identifiye ederek tedavide en doğru yaklaşım olarak antibiyotik duyarlılıklarını belirlemek amaçlanmıştır. Bu amaçla Kütahya ilindeki mezbahalarda kesilen sığırlardan 210 akciğer örneği alındı. Örneklerden kanlı agar, Eosin methylene blue agar ve MacConkey agara ekimler gerçekleştirildi ve 89 (%42) Gram negatif bakteri izole edildi. Pasteurella türlerinin özelliklerini gösteren 17 bakteri otomatize Vitek 2 cihazında identifiye edildi. İdentifikasyon sonucu iki (%0,95) izolatun Pasteurella multocida olduğu tespit edildi. İzolatların antibiyotik duyarlılıkları Kirby-Bauer disk difüzyon testi kullanılarak araştırıldı. İzolatların amoksisilin klavulanik asit, enrofloksasin, siprofloksasin ve seftiofura %100 oranında duyarlı, danofloksasine ise %50 duyarlı %50 artırılmış dozajda duyarlı olduğu gözlemlenirken, sülfametaksazol-trimetoprim ve tetrasikline %100 oranında dirençli olduğu tespit edildi.

Keywords

Antibiyotik duyarlılık testi, sığır, kesimhane, Pasteurella türleri

Isolation, Identification and Antibiotic Sensitivity of Pasteurella Species In Cattle Slaughtered in Slaughterhouses in Kütahya Province

Abstract

In this study, it was aimed to isolate and identify Pasteurella species, which is one of the most important bacterial agents that cause bovine pneumonia, and to determine their antibiotic susceptibility as the most accurate approach in treatment. For this purpose, 210 lung samples were taken from cattle slaughtered in slaughterhouses in Kütahya. Sowing was performed on blood agar, Eosin methylene blue agar and MacConkey agar from the samples and 89 (42%) Gram negative bacteria were isolated. 17 bacteria showing the characteristics of Pasteurella species were identified in the automated Vitek 2 device. As a result of the identification, two (0.95%) isolates were found to be Pasteurella multocida. Antibiotic susceptibility of the isolates was investigated using the Kirby-Bauer disk diffusion test. It was observed that the isolates were 100% sensitive to amoxicillin clavulanic acid, enrofloxacin, ciprofloxacin and ceftiofur, 50% sensitive to danofloxacin, 50% sensitive at increased dosage, and 100% resistant to sulfamethoxazole-trimethoprim and tetracycline.

Keywords

Antibiotic sensitivity test, cattle, slaughterhouse, Pasteurella species

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