Sideritis akmanii'den Elde Edilen Sulu ve Etanol Ekstraktlarının Antioksidan ve Histopatolojik Etkilerinin Araştırılması

Year 2023, Volume: 16 Issue: 3, 383 - 392, 30.09.2023

İsmail Küçükkurt , Fahriye Kan , Sinan İnce , Hasan Hüseyin Demirel , Erten Akbel , Damla Arslan Acaröz

Abstract

Bu çalışmada, Afyonkarahisar ili Kumalar yaylası bölgesinde Şuhut-Sandıklı yolu üzerinde yayılış gösteren endemik Sideritis akmanii bitkisinden elde edilen sulu ve etanolik ekstraktların antioksidan aktivitelerinin ve histopatolojik etkilerinin araştırılması amaçlanmıştır. 48 adet Wistar albino erkek rat 8 gruba ayrıldı; Kontrol Grubu (Grup I), CMC Grubu (Grup II), %0,5 CMC (karboksimetil selüloz), Sulu ve etanolik bitki ekstrakt grupları (Grup III-IV-V-VI-VII-VIII). Belirlenen LD50 dozundan uygun oranlarda üç farklı doz (%1, %2 ve %4) ekstraktı 6 deney grubuna 30 gün uygulandı. Deney sonunda sağlıklı ratların kan ve doku (karaciğer, kalp, beyin, böbrek, testis) örneklerinde malondialdehit (MDA), glutatyon (GSH) düzeyleri, süperoksit dismutaz (SOD) ve katalaz (CAT) enzim aktiviteleri ölçüldü.Tam kan MDA düzeyi etanol ekstrakt grubunun tüm dozlarında kontrol grubuna göre azalırken, GSH düzeyi her iki ekstrakt grubunda da kontrol grubuna göre arttı. Etanol gruplarında eritrosit ve testis dokusu dışında SOD ve CAT enzim aktiviteleri kontrol grubuna göre arttı (p<0.05). Histopatolojik inceleme sonuçlarında ise herhangi bir değişiklik gözlenmedi. Çalışma sonucunda Sideritis akmanii'nin sulu ve etanol ekstraktlarının antioksidan özelliklere sahip olduğu ve etanol ekstraktının antioksidan enzimler üzerinde daha etkili olduğu gözlemlendi.

Keywords

Antioksidan enzimler, Histopatoloji, Lipid peroksidasyonu, Sideritis akmanii ekstraktı (sulu ve etanollü)

Project Number

Project no: 18.KARIYER.190

Investigation of Antioxidant and Histopathological Effects of Aqueous and Ethanol Extracts Obtained from Sideritis akmanii

Abstract

In this study, it was aimed to investigate the antioxidant activities and histopathological effects of the aqueous and ethanolic extracts obtained from the endemic Sideritis akmanii plant, which spreads on the Şuhut-Sandıklı road in the Kumalar plateau region of Afyonkarahisar province. The number of 48 Wistar albino male rats were divided into 8 groups; Control Group (Group I), CMC Group (Group II), 0.5% CMC (carboxymethyl cellulose), Aqueous and ethanolic plant extract groups (Group III-IV-V-VI-VII-VIII). Three different doses (1%, 2%, and 4%) extracts from the determined LD50 dose were applied to 6 experimental groups for 30 days. At the end of the experiment, malondialdehyde (MDA), glutathione (GSH) levels, superoxide dismutase (SOD), and catalase (CAT) enzyme activities were measured in healthy rat's blood and tissue (liver, heart, brain, kidney, testis) samples. The whole blood MDA level decreased in all doses of the ethanolic extract group compared to the control group, while the GSH level increased in both extract groups compared to the control group. SOD and CAT enzyme activities were increased in the ethanolic groups compared to the control group, except for erythrocyte and testis tissue (p<0.05). On the other hand, no changes were observed in the histopathological examination results. As a result of the study, it was observed that the aqueous and ethanolic extracts of Sideritis akmanii had antioxidant properties and the ethanolic extract was more effective on antioxidant enzymes.

Keywords

Antioxidant enzymes, Histopathology, Lipid peroxidation, Sideritis akmanii extract (aqueous and ethanolic)

Supporting Institution

This study was supported by Afyon Kocatepe University Scientific Research Council, Afyonkarahisar, Turkey. All authors express their gratitude to this institution.

Project Number

Project no: 18.KARIYER.190

Thanks

We thank Prof Dr. Mustafa KARGIOGLU for identifying the Sideritis akmanii used in the study

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