Investigation on Antimicrobial Resistance Levels of Escherichia coli Strains Isolated from Bovine Fecal Samples and Comparison with Guidelines of the Clinical Laboratory Standards Institute (CLSI) and European Committee on Antimicrobial Susceptibility Testing (EUCAST)

Year 2023, Volume: 16 Issue: 3, 334 - 341, 30.09.2023

Recep Kalın , Burcu Karagülle , Mehmet Nuri Açık , Murat Karahan , Yasin Öztürk Burhan Çetinkaya

https://doi.org/10.30607/kvj.1302078

Abstract

This study was carried out to investigate the antibiotic resistance levels of Escherichia coli isolates of bovine origin and to compare the results with CLSI and EUCAST guideline values. For this purpose, 97 E. coli strains isolated from fecal samples of cattle in 12 different farms were tested against 32 antibiotics by using the disk diffusion method. The zone diameters of 13 antibiotics examined within the scope of the study were compared according to the CLSI and EUCAST 2020 guidelines, and their consistency levels were evaluated statistically. The highest resistance rates in E. coli isolates were determined against tetracycline (68%), streptomycin (63.9%), ampicillin (58.8%), and doxycycline (50.5%) antibiotics. On the other hand, the isolates were found to be highly susceptible to amikacin and cephalosporin group antibiotics. When CLSI and EUCAST guidelines were compared, it was found that there were statistically significant differences between the resistance rates of nitrofurantoin, gentamicin, and amikacin. Only 10 (10.3%) of the isolates were detected to be susceptible to all the antibiotics tested, whereas 17.5% were resistant to 10 or more antibiotics. The results of this study showed that E. coli isolates of bovine origin were highly resistant against antibiotics used in the field for a long period, especially the number of isolates with multiple antibiotic resistance was striking. It was concluded that due to substantial inconsistencies between the CLSI and EUCAST guidelines for some antibiotics such as amikacin, nitrofurantoin, and gentamicin, there is an urgent need to execute necessary updates in both guidelines.

Keywords

Bovine, Escherichia coli, Antibiotic resistance, CLSI, EUCAST

Sığır Dışkı Örneklerinden İzole Edilen Escherichia coli Suşlarının Antimikrobiyal Direnç Düzeylerinin Araştırılması ve Klinik Laboratuvar Standartları Enstitüsü (CLSI) ve Avrupa Antimikrobiyal Duyarlılık Testi Komitesi (EUCAST) Kılavuzlarıyla Karşılaştırılması

Abstract

Bu çalışma, sığır orijinli Escherichia coli izolatlarının antibiyotik direnç düzeylerinin araştırılması ve sonuçların CLSI ve EUCAST kılavuz değerleri ile karşılaştırılması amacıyla yapılmıştır. Bu amaçla 12 farklı işletmedeki sığırların dışkı örneklerinden izole edilen 97 E. coli suşu, disk difüzyon yöntemi kullanılarak 32 antibiyotiğe karşı test edilmiştir. Çalışma kapsamında incelenen 13 antibiyotiğin zon çapları CLSI ve EUCAST 2020 kılavuzlarına göre karşılaştırıldı ve tutarlılık düzeyleri istatistiksel olarak değerlendirildi. E. coli izolatlarında en yüksek direnç oranları tetrasiklin (%68), streptomisin (%63,9), ampisilin (%58,8) ve doksisiklin (%50,5) antibiyotiklerine karşı saptanmıştır. Öte yandan, izolatların amikasin ve sefalosporin grubu antibiyotiklere karşı yüksek duyarlı olduğu saptanmıştır. CLSI ve EUCAST kılavuzları karşılaştırıldığında nitrofurantoin, gentamisin ve amikasin direnç oranları arasında istatistiksel olarak anlamlı farklar olduğu görüldü. İzolatların sadece 10'unun (%10,3) test edilen tüm antibiyotiklere duyarlı olduğu, %17,5'inin ise 10 ve daha fazla antibiyotiğe dirençli olduğu saptandı. Bu çalışmanın sonuçları sığır orijinli E. coli izolatlarının uzun süredir sahada kullanılan antibiyotiklere karşı oldukça dirençli olduğunu göstermiştir, özellikle çoklu antibiyotik direncine sahip izolatların sayısı dikkat çekicidir. Amikasin, nitrofurantoin ve gentamisin gibi bazı antibiyotikler için CLSI ve EUCAST kılavuzları arasındaki önemli tutarsızlıklar nedeniyle, her iki kılavuzda da gerekli güncellemelerin acilen yapılması gerektiği sonucuna varılmıştır.

Keywords

Sığır, Escherichia coli, Antibiyotik direnci, CLSI, EUCAST

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