Ratlarda Barsak Ensizyon Yarası İyileşmesi Üzerine Bor’un Etkisinin Araştırılması

Year 2024, Volume: 17 Issue: 4, 401 - 411

Yakup Alarslan , Zülfükar Sarıtaş

https://doi.org/10.30607/kvj.1528843

Abstract

u çalışmada 250-300 g, 38 erişkin erkek Wistar Albino rat kullanıldı, rastgele olarak Bor verilen dört grup (n=7/grup) ve iki kontrol grubu (n=5/grup) olmak üzere altı gruba ayrıldı. Etibor-48 (Boraks pentahidrat) serum fizyolojik içinde seyreltildi ve gavaj yoluyla uygulandı. Bor 1 grubuna üç gün 10 mg/kg bor, Bor 2 grubuna yedi gün 10 mg/kg bor, Bor 3 grubuna üç gün 30 mg/kg bor ve Bor 4 gruba, çalışmanın başlamasından yedi gün önce 30 mg/kg bor verildi. 0. günde tüm ratlara genel anestezi altında kolon anastomozu yapıldı. Ameliyat sonrası kontrol gruplarına hiçbir ilaç uygulanmadı ve postoperatif üçüncü ve yedinci gün ötenazi yapıldı. MPO, IL-1, IL-6, TNF-α, MDA, NO, GSH ve AOA'yı ölçmek için nekropsi sırasında tüm sıçanlardan kardiyak punksiyonla kan örneği alındı. Anastomoz bölgesi histopatolojik olarak değerlendirildi. Bor 1, 2, 3 ve 4 grupları ile Kontrol 3 ve 7 günlük gruplarda inflamatuar hücre ölçüm sonuçları sırasıyla 2,93±0,41, 3,93±0,41, 3,61±0,55, 3,93±0,41, 1,26±0,41 ve 1,43±. 0.51 olarak bulundu. Gruplar arasında istatistiksel farklar vardı (P˂0.05). Bor 1, 2, 3 ve 4 grupları ile Kontrol 3 ve 7 günlük gruplarda AOA ölçüm sonuçları sırasıyla 7,38±0,64, 8,27±0,57, 9,07±1,16, 9,06±0,86, 10,00±1,47 ve 9,86±0,54 mmol/l idi. Bor 1 ve Kontrol 3- ve 7 günlük gruplar arasında istatistiksel fark vardı (P˂0.05). 30 mg/kg Boraks pentahidrat solüsyonunun bağırsak yara iyileşmesi üzerine literatürün aksine olumlu etkisi olduğu sonucuna varılmıştır.

Keywords

Antioksidan aktivite, barsak ensizyonel yara iyileşmesi, Borax pentahydrate, sıçan.

Project Number

AKÜ BAPK 18.Sağ.Bil.19

The Investigation of the Effect of Boron on Intestinal Incision Wound Healing in Rats

Abstract

In this study 250-300 g 38 adult male Wistar Albino rats were used, randomly divided into six groups as four groups that received boron (n=7/group) and two control groups (n=5/group). Etibor-48 (Borax pentahydrate) was diluted in saline and administered by gavage. The Boron 1 group received 10 mg/kg boron for three days and the Boron 2 group received 10 mg/kg for seven days, Boron 3 group received 30 mg/kg for three days, and the Boron 4 group, received 30 mg/kg for seven days before the start of the study. On day 0, colon incisional wound was performed for all rats under general anesthesia and sutured. Control groups took no drugs and were euthanized on the third and seventh postoperative days. Blood sampling were done via cardiac punction at necropsy to measure Interleukin-1, IL-6, TNF-α, MPO, MDA, NO, GSH, and AOA. The sutured bowel incision line was evaluated histopathologically. Inflammatory cell measurement results in Boron 1, 2, 3, and 4 groups, and Control 3- and 7-day groups were 2.93±0.41, 3.93±0.41, 3.61±0.55, 3.93±0.41, 1.26±0.41, and 1.43±0.51, respectively. There were statistical difference between the groups (P˂0.05). AOA measurement results in the Boron 1, 2, 3, and 4 groups, and Control 3- and 7-day groups were 7.38±0.64, 8.27±0.57, 9.07±1.16, 9.06±0.86, 10.00±1.47, and 9.86±0.54 mmol/L, respectively. There were statistical difference between the Boron 1 and the Control 3- and 7-day groups (P˂0.05). It is concluded that Borax pentahydrate solution 30 mg/kg had a positive effect on intestinal incisional wound healing, contrary to the literature.

Keywords

Antioxidant activity, Borax pentahydrate, intestinal incisional wound healing, rat.

Supporting Institution

AKÜ BAPK

Project Number

AKÜ BAPK 18.Sağ.Bil.19

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