Investigation of Anti-Müllerian Hormone Presence in Bitch Urine and Comparison of Anti-Müllerian Hormone Levels in Blood Serum and Urine

Year 2024, Volume: 17 Issue: 4, 418 - 422

Semra Kaya , Murat Can Demir , İnan Kaya , Muhammet Ali Karadağ , Gökhan Koçak , Cihan Kaçar

Abstract

The presented study aimed to investigate the presence of AMH in bitch urine and to determine whether there is a correlation between blood serum and urine AMH levels. Forty-two healthy crossbreed bitches brought to Kafkas University, Faculty of Veterinary Medicine, Animal Hospital, Department of Obstetrics and Gynecology with a request for ovariohysterectomy were included in the study. Blood samples were taken from the Vena cephalica antebrachii, and urine samples were collected using a urinary catheter of all bitches. After the blood and urine samples were centrifuged, AMH levels were determined using the ELISA method. The average AMH concentration was determined to be 4.56±0.53 ng/mL in urine and 7.75±1.19 ng/mL in blood serum. No significant correlation was found between blood and urine AMH levels (P>0.05). It was concluded that urine AMH levels were not related to blood serum AMH levels.

Keywords

Anti-Müllerian Hormone, Bitch, Blood serum, Urine

Köpek İdrarında Anti-Müllerian Hormon Varlığının Araştırılması ve Kan Serumu ve İdrar Anti-Müllerian Hormon Düzeylerinin Karşılaştırılması

Abstract

Sunulan çalışmada köpek idrarında AMH varlığının araştırılması ve kan serumu ile idrar AMH düzeyi arasında korelasyon olup olmadığının belirlenmesi amaçlanmıştır. XXX Üniversitesi, Veteriner Fakültesi Hayvan Hastanesi, Doğum ve Jinekoloji Kliniği’ne ovaryohisterektomi isteğiyle getirilen sağlıklı 42 melez ırk köpek çalışmaya dahil edildi. Tüm köpeklerin Vena cephalica antebrahii’den kan örnekleri alındı ve idrar katateri kullanılarak idrar örnekleri toplandı. Kan ve idrar örnekleri santrifüj edildikten sonra AMH düzeyleri ELISA yöntemiyle belirlendi. Kan serumunda ortalama AMH düzeyi 7,75±1,19 ng.ml-1, idrarda ise 4,56±0,53 ng.ml-1 bulundu. Kan ve idrar AMH düzeyi arasında anlamlı bir korelasyon saptanmadı. Sonuç olarak bu çalışmada köpeklerde idrar AMH düzeyi ilk defa ölçülmüş ve kantitatif veri olarak sunulmuştur. İdrar AMH düzeyinin kan serumundaki AMH düzeyi ile ilişkili olmadığı ve idrar AMH düzeyine bakılarak köpeklerde ovaryum rezervi hakkında tahminde bulunmanın yanıltıcı olabileceği kanaatine varıldı.

Keywords

Anti-Müllerian hormon, İdrar, Kan serumu, Köpek

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