Variability of CAPN1 g.5709 C>G and MYF5 g.1911 A>G Polymorphisms in Beef Cattle Imported from Brazil to Turkey

Year 2019, Volume: 59 Issue: 2, 72 - 78, 13.12.2019

Sena Ardıçlı , Hakan Ustüner Öznur Arslan Oğuz Kandazoğlu

Abstract

The objective of the present study was to determine genotypic/allelic frequencies and population genetic indices of CAPN1 g.5709 C>G and MYF5 g.1911 A>G polymorphisms in beef cattle imported from Brazil to Turkey. Single nucleotide polymorphisms were carried out using the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis. A total of 108 bulls, including Angus, Angus×Hereford×Nellore, Brahman, Hereford, Limousine, and Charolais breeds, were genotyped. Concerning the CAPN1, CC genotype was not found in this study. Besides, the G allele frequency was quite high (0.75). Regarding the MYF5, frequency of AA genotype was rather low (4.63%) compared to the other two genotypes, AG and GG. Therefore, the frequency of A allele was quite low (0.21). On the basis of breed-specific evaluation, the highest frequency of GG genotype of the CAPN1 was found in Brahman breed (88.24%) whereas the highest frequency of heterozygous genotype was determined in Charolais breed (85.71%). The frequency of MYF5 GG genotype was found to be very high in Limousine breed (75.00%). Moreover, MYF5 AA genotype was absent in Angus, Brahman, Limousine, and Charolais breeds. The present study may be useful for further genetic analyses conducted on beef cattle imported into Turkey.

Keywords

Beef cattle, CAPN1, MYF5

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