Zebra Balığının (Dania rerio) Diyetsel Protein Gereksinimi

Öz

Ortalama başlangıç ağırlığı 88,61±0,82 mg olan zebra balıkları (Danio rerio) protein düzeyi % 20-55 arasında değişen sekiz adet izoenerjitik yemle beslenmiştir. Her bir deneme yemi üç tekrarlı olarak 6 hafta boyunca balıklara verilmiştir. Spesifik büyüme oranı (SGR) 2. ve 4. haftalarda kuadratik olarak etkilenirken, bu eğilim deneme sonunda kaybolmuştur. Protein düzeyleri arttıkça yem tüketimi, yemden yararlanma oranı ve protein etkinlik oranı doğrusal olarak düşmüştür. Tüm vücut kuru madde, kül ve lipit konsantrasyonları diyetsel protein düzeyinin artışı ile doğrusal olarak düşmüş, vücut protein düzeyi ise kuadratik olarak etkilenmiştir. Diyetsel protein gereksinimlerini tahmin etmek için, ikinci derece regresyon ve iki kırıklı linear model (İKLM) kullanılmıştır. 4. hafta ve deneme sonu SGR değerleri kullanıldığında İKLM daha düşük kalıntı kareler toplamı vermiştir. 4. hafta ve final SGR oranlarına göre, İKLM minimum protein gereksinimlerini sırasıyla, % 27,69 ve % 28,93 olarak tahmin etmiştir. Kısaca, çalışma bulguları zebra balıklarının maksimum büyüme için minimum protein gereksinimlerinin yaklaşık % 29 olduğunu göstermektedir.

Anahtar Kelimeler

• Zebra balığı,diyetsel protein,büyüme,yemleme

Dietary Protein Requirements of Zebrafish (Dania rerio)

Abstract

Zebrafish (Danio rerio) with an initial weight of 88.61±0.82 mg were fed eight isoenergetic diets containing dietary protein levels ranging from 20 to 55 % by 5 % increments. Each diet was feed in triplicate of fish for 6 weeks. Specific growth rates (SGR) at week 2 and 4 were quadratically affected by the treatments but this trend disappeared at the end of the experiment. Dietary protein levels linearly reduced the values of daily feed intake, feed conversion ratio and protein efficiency rate. The whole body dry matter, ash and lipid concentrations linearly decreased with dietary protein levels whereas whole body protein was quadratically affected. The second order polynomial and two break point linear models (TBPLM) were used to estimate dietary protein requirements. The later model generated lower residual sum of squares when SGR_Week4 and SGR_Final valueswere used as a response. Minimum dietary protein requirements for SGR_Week4 and SGR_Final were estimated by the TBPLM as 27.69 and 28.93 % respectively. Briefly, results of the study suggest a minimum dietary protein requirement of zebrafish is about 29 % for maximum growth rate.

Keywords

• Zebrafish,dietary protein,growth,feeding

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