Doğu Karadeniz Bölgesinde Amfibi Patojeni Batrachochytrium dendrobatidis’in Potansiyel Dağılımı

Öz

Amfibi patojeni Batrachochytrium dendrobatidis Anadolu’da rapor edilmesine rağmen, hem coğrafik dağılımı hem de Türkiye amfibileri üzerindeki etkisi hala belirsizdir. Bu çalışmada, Doğu Karadeniz Bölgesindeki beş sulak alandan on farklı amfibi türlerine (Pelodytes caucasicus, Rana dalmatina, Rana macrocnemis, Bufo bufo, Bufo verrucosissimus, Bufotes variabilis, Hyla savignyi Pelophylax ridibundus, Ommatotriton ophryticus, Mertensiella caucasicus) ait 62 ergin birey toplandı. Kantitatif Real-time PCR tekniği ile tüm bireylerde B. dendrobatidis enfeksiyonlarının prevalansı ve yoğunluğu ile araştırıldı ve Doğu Karadeniz Bölgesi’ndeki 10 amfibi türünden toplanan 62 bireyin 13’ ünde B. dendrobatidis enfeksiyonu varlığı ilk kez rapor edildi. Enfeksiyon yoğunluğu 403,520-534,340 genomik eşdeğerler arasında değiştiği belirlendi. Amfibi türler arasında en yüksek genomik eşdeğer Uzungöl’ deki P. caucasicus  (534,280) ve Karagöl'de B. bufo (504,00) saptandı.

Anahtar Kelimeler

• Chytridiomycosis,Batrachochytrium dendrobatidis,Anadolu,Amphibia

Potential Distribution of the Amphibian Pathogen, Batrachochytrium dendrobatidis in the Eastern Black Sea Region of Turkey

Abstract

Although Batrachochytrium dendrobatidis, pathogen for amphibians, has been reported in Anatolia, its geographical distribution, as well as its impact on the amphibians in Turkey, remained obscure. In this study, 62 adult individuals belonging to ten different species (Pelodytes caucasicus, Rana dalmatina, Rana macrocnemis, Bufo bufo, Bufo verrucosissimus, Bufotes variabilis, Hyla savignyi, Pelophylax ridibundus, Ommatotriton ophryticus, and Mertensiella caucasicus) were collected from five wetland habitats in Eastern Black Sea Region of Turkey. The prevalence and the intensity of B. dendrobatidis infections in all the individuals were investigated by using quantitative Real-time-PCR technique and the presence of B. dendrobatidis infection was reported for the first time in 13 of the 62 individuals collected from 10 amphibian species from Eastern Black Sea Region of Turkey. The intensity of B. dendrobatidis infection ranged from 403.520 to 534.280 genomic equivalents (GE) was detected. The highest GE between amphibian species were determined in P. caucasicus (534.280 GE) in Uzungöl (Çaykara-Trabzon) and B. bufo (504.00 GE) in Lake Karagöl (Şavşat-Artvin).

Keywords

• Chytridiomycosis,Batrachochytrium dendrobatidis,Anatolia,Amphibia

References

1. Anna ES, Grismer LL, Anuar S, Onn CK, Grismer JL, Quah E, Muin MA, Ahmad N, Lenker M, Zamudio KR. 2011. First record of Batrachochytrium dendrobatidis infecting four frog families from Peninsular Malaysia. Ecohealth. 8(1):121-128. doi: 10.1007/s10393-011-0685-y
2. Ansell SW, Stenøien HK, Grundmann M, Russell SJ, Koch MA, Schneider H, Vogel JC. 2011. The importance of Anatolian mountains as the cradle of global diversity in Arabis alpina, a key arctic–alpine species. Annals of Botany. 108(2):241-252. doi: 10.1093/aob/mcr134
3. Bai C, Garner TWJ, Garner, LiY. 2010. First evidence of Batrachochytrium dendrobatidis in China: discovery of chytridiomycosis in introduced American bullfrogs and native amphibians in the Yunnan Province, China. EcoHealth. 7(1):127-134. doi: 10.1007/s10393-010-0307-0
4. Başkale E, Yildirim E,Çevik IE, Kaya U. 2013. Population size and age structure of metamorphic and pedomorphic forms of Ommatotriton ophryticus (Berthold, 1846) in the Northwestern Black Sea Region of Turkey. J Herpetol. 47(2):270-276. doi: 10.1670/11-116
5. Boyle DG, Boyle DB, Olsen V, Morgan JAT, Hyatt AD. 2004. Rapid quantitative detection of chytridiomycosis Batrachochytrium dendrobatidis in amphibian samples using real-time taqman PCR assay. Dis Aquat Organ. 60(2):141-148. doi: 10.3354/dao060141
6. Bülbül U, Matsui M, Kutrup B, Eto K. 2011. Taxonomic relationships among Turkish water frogs as revealed by phylo-genetic analyses using mtDNA gene sequences. Zool Sci. 28(12):930-936. doi: 10.2108/zsj.28.930
7. Carver S, Bell BD, Waldman B. 2010. Does chytridiomycosis disrupt amphibian skin function? Copeia. 2010(3):487-495. doi: 10.1643/CH-09-128
8. Daszak P, Berger L, Cunningham AA, Hyatt AD, Green DE, Speare R. 1999. Emerging infectious diseases and amphibian population declines. Emerg Infect Dis. 5(6):735-748. doi : 10.3201/eid0506.990601

9. Di Rosa I, Simoncelli F, Fagotti A, Pascolini R. 2007. Ecology: the proximate cause of frog declines? Nature. 447(7144):4-5. doi: 10.1038/nature05941
10. Erismis UC, Konuk M, Yoldas T, Agyar P, Yumuk D, Korcan SE. 2014. Survey of Turkey’s endemic frogs for amphibian chytrid fungus Batrachochytrium dendrobatidis. Dis Aquat Organ. 111(2):153-157. doi: 10.3354/dao02742
11. Farrer RA, Weinert LA, Bielby J, Garner TW, Balloux F, Clare F, Bosch J, Cunningham AA, Weldon C, du Preez LH, Anderson L, Pond SL, Shahar-Golan R, Henk DA, Fisher MC. 2011. Multiple emergences of genetically diverse amphibian-infecting chytrids include a globalized hypervirulent recombinant lineage. P Natl Acad Scı Usa. 108(46):18732-18736. doi: 10.1073/pnas.1111915108
12. Fisher MC, Garner TW, Walker SF. 2009. Global emergence of Batrachochytrium dendrobatidis and amphibian chytridiomycosis in space, time, and host. Annu Rev Microbiol. 63:291-310. doi: 10.1146/annurev.micro.091208.073435
13. Franzen M, Bussmann M, Kordges T, Thiesmeier B. 2008. Die Amphibien und Reptilien der Südwest-Türkei. Bielefeld: Laurenti Verlag 329 p.
14. Göçmen B, Veith M, Iğci N, Akman B, Godmann O, Wagner N. 2013. No detection of the amphibian pathogen Batrachochytrium dendrobatidis in terrestrial Turkish salamanders (Lyciasalamandra) despite its occurrence in syntopic frogs (Pelophylax bedriagae). Salamandra. 49(1):51-55.
15. Hyatt AD, Boyle DG, Olsen V, Boyle DB, Berger L, Obendorf D. 2007. Diagnostic assays and sampling protocols for the detection of Batrachochytrium dendrobatidis. Dis Aquat Organ. 73(3):175-192. doi: 10.3354/dao073175
16. Kriger KM, Hines HB, Hyatt AD, Boyle DG, Hero JM. 2006. Techniques for detecting chytridiomycosis in wild frogs: comparing histology with real-time taqman PCR. Dis Aquat Organ. 71(2):141-148. doi: 10.3354/dao071141
17. Kriger KM, Pereoglou F, Hero JM. 2007. Latitudinal variation in the prevalence and intensity of chytrid Batrachochytrium dendrobatidis infection in eastern Australia. Conserv Biol. 21(5):1280-1290. doi: 10.1111/j.1523-1739.2007.00777.x
18. Marantelli G, Berger L, Speare R, Keegan L 2004. Distribution of the amphibian chytrid Batrachochytrium dendrobatidis and keratin during tadpole development. Pacific Conservation Biology. 10(2):173-179. doi: 10.1071/PC040173
19. Mazzoni R, Cunningham AA, Daszak P, Apolo A, Perdomo E, Speranza G. 2003. Emerging pathogen of wild amphibians in frogs.Rana catesbeiana farmed for international trade. Emerg Infect Dis. 9(8):995-998. doi: 10.3201/eid0908.030030
20. Morehouse EA, James TY, GanleyA RD, Vilgalys R, Berger L, Murphy PJ, Longcore J E. 2003. Multilocus sequence typing suggests that the chytrid pathogen of amphibians is a recently emerged clone. Mol Ecol. 12(2):395-403. doi: 10.1046/j.1365-294X.2003.01732.x
21. Richmond JQ, Savage AE, Zamudio KR, Rosenblum EB. 2009. Toward immunogenic studies of amphibian chytridiomycosis: linking innate and acquired immunity. Bioscience 59(4):311-32. doi: 10.1525/bio.2009.59.4.9
22. Ron SR. 2005. Predicting the distribution of the amphibian pathogen Batrachochytrium dendrobatidis in the new world. Biotropica. 37(2):209-221. doi: 10.1111/j.1744-7429.2005.00028.x
23. Sensoy S, Demircan M, Ulupınar Y, Balta İ. 2008. Türkiye İklimi; [cited 18 Feb 2018]. Available from https://www.mgm.gov.tr/FILES/genel/makale/13_turkiye_iklimi.pdf
24. Şekercioğlu CH, Anderson S, Akçay E, Bilgin R, Emre Can Ö, Semiz G, Tavşanoğlu C, Baki Yokeş M, Soyumert A, İpekdal K, Sağlam İK, Yücel M, Dalfes HN. 2011. Turkey’s globally important biodiversity in crisis. Biol Conserv. 144(12):2752-2769. doi:10.1016/j.biocon.2011.06.025 Veith M, Schmidtler F, Kosuch J, Baran I, Seitz A. 2003. Paleoclimatic changes explain Anatolian mountain frog evolution: a test for alternating vicariance and dispersal event. Mol Ecol. 12(1):185-189. doi: 10.1046/j.1365-294X.2003.01714.x
25. Weldon C, du Preez LH, Hyatt AD, Muller R, Speare R. 2004. Origin of the amphibian chytrid fungus. Emerg Infect Dis. 10(12):2100-2105. doi: 10.3201/eid1012.030804
26. Yildiz MZ, Göcmen B. 2012. Population dynamics, reproduction, and life history traits of Taurus Frog, Rana holtzi Werner, 1898 (Anura: Ranidae) in Karagöl (Ulukışla, Niğde), Turkey. Herpetologica Romanica.6:1-40.