Döllenmemiş Pullu Sazan (Cyprinus carpio) Yumurtalarının İn Vitro Muhafaza Edilmesi: Sulandırıcı ve Muhafaza Sürelerinin Etkisi

Abstract

Bu çalışma, farklı sulandırıcı ve in vitro muhafaza sürelerinin pullu sazan (Cyprinus carpio) yumurtalarının döllenme, gözlenme ve açılma başarısı üzerine olan etkilerinin araştırılması amacıyla yürütülmüştür. 20-ml hacmindeki üç farklı sulandırıcı (Ringer, Dettlaff ve Cortland) ve ovaryum sıvısı ile 15-cm çaplı petri kutularında muamele edilen yaklaşık 200 yumurta, 22.5⁰C'de 30, 60 ve 90 dk süre ile muhafaza edildi. İn vitro muhafaza edilen yumurtalar, muhafaza süresinin sonunda %80'den fazla motil özelliğe sahip olan 50 μl sperma ile her petri kutusunda döllenmiştir. En yüksek fertilizasyon oranları, Cortland solüsyonunda 60 dk ve ovaryum sıvısında 90 dk muhafaza edilen yumurta örneklerinde sırasıyla %86 ve %72 olarak belirlenmiştir (p<0,05). En yüksek gözlenme oranı Cortland solüsyonunda 60 dk muhafaza edilen yumurta örneklerinde %80 olarak belirlenmiştir (p<0,05). En iyi açılma oranı ovaryum sıvısında 30 dk muhafaza edilen yumurta örneklerinde %60 olarak belirlenmesine rağmen, 90 dk muhafaza süresinde ise en iyi sonuç Cortland solüsyonunda %48 olarak elde edildi (p<0,05). Elde edilen sonuçlar, Cortland solüsyonunun en iyi sulandırıcı olduğunu ve pullu sazan yumurtalarının in vitro muhafazasında ovaryum sıvısı yerine ikame edilebileceğini göstermektedir.

Keywords

• Sulandırıcı,yumurta,kısa süreli muhafaza,döllenme

In Vitro Storage of Unfertilized Eggs of Scaly Carp (Cyprinus carpio) : Effect of Different Artificial Mediums and Storage Periods

Abstract

The present study was carried out in order to explore the effect of different artificial mediums and in vitro storage periods on fertilization, eyeing and hatching success of scaly carp (Cyprinus carpio) eggs. The batches of about 200 pooled eggs treated with 20-ml three different extenders (Ringer, Dettlaff and Cortland) and Ovarian fluid in 15-cm petri dishes, were stored at 22.5⁰C for 30, 60, or 90 min. The in vitro stored eggs were fertilized by adding of 50 μl sperm which showing motility higher than 80%, in each petri dishes end of the storage period. The highest fertilization rates were determined as 86% and 72% with the egg samples stored for 60 min in Cortland solution and for 90 min in Ovarian fluid respectively (p<0.05). The highest eyeing rate (80%) was determined in egg samples kept in Cortland solution for 60 min storage (p<0.05). Despite the best hatching rate (60%) of the egg samples determined with Ovarian fluid at 30 min storage, the Cortland solution was (48%) the best for 90 min (p<0.05). Results indicate that Cortland solution is the most suitable extender and can be substituted instead of Ovarian fluid for in vitro storage of scaly carp eggs.

Keywords

• Extender,egg,short-term preservation,fertilization

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