Do Dietary Synbiotics Modulate Growth Performance and Haematological Properties of Tilapia, Oreochromis niloticus ?

Abstract

A 60-day long aquarium trial was conducted in laboratory condition to assess the growth performance of tilapia Oreochromis niloticus fed with various types of synbiotic diets. Twenty-five homogenous fry were randomly assigned into four different treatments with three replications. Fish were fed with experimental diets containing 0.3% probiotic (PBO)+1.5% molasses, 0.3% PBO+1.5% yeast and 0.3% PBO+1.5% sugar and marked as T1 T4 (control), T2, T3 and T4 and fish in control were feed with commercial diet. Length and weight were recorded every 10 days and at the end of the trial blood samples were extracted for haematological analysis. The T4 group showed significantly higher growth performance among the treatments and differences between T1 and T2 were found as non-significant (P<0.05). Again, significantly superior Specific Growth Rate (SGR %) was found in T4 and non-significant differences detected between T2 and T3 as well (P>0.05). Haematological analysis showed significant changes among the treatments for hemoglobin, Red Blood Cell (RBC) count and White Blood Cell (WBC) count (P>0.05). All treatments showed better growth performance in contrast to the control group. Finally, present results indicate that synbiotics might increase the growth performances, haematological indices and health condition in O. niloticus.

Keywords

• Synbiotics
• probiotics
• growth performence
• hematological properties
• O. niloticus

References

1. Ahmed I, Reshi QM, Fazio F. 2020. The influence of the endogenous and exogenous factors on hematological parameters in different fish species: a review. Aqua. Intl. 28(3):869–899. doi: 10.1007/s10499-019-00501-3
2. Ai Q, Xu H, Mai K, Xu W, Wang J, Zhang W. 2011. Effects of dietary supplementation of Bacillus subtilis and fructooligosaccharide on growth performance, survival, non-specific immune response and disease resistance of juvenile large yellow croaker, Larimichthys crocea. Aquaculture, 317(1):155–161. doi: 10.1016/j.aquaculture.2011.04.036
3. Akrami R, Iri Y, Rostami HK, Mansour MR. 2013. Effect of dietary supplementation of fructooligosaccharide (FOS) on growth performance, survival, lactobacillus bacterial population and hemato-immunological parameters of stellate sturgeon (Acipenser stellatus) juvenile. Fish Shellfish Immunol. 35(4):1235-1239.
4. Akter M, Iqbal M, Hossain M, Rahman A, Uddin S. 2019. Effect of L-Arginine on the growth of monosex fingerling nile tilapia (Oreochromis niloticus L.). J. Fish. Life. Sci, 4(2):31–36.
5. Alam MB, Islam MA, Marine SS, Rashid A, Hossain MA. 2014. Growth performances of GIFT tilapia (Oreochromis niloticus) in Cage culture at the Old Brahmaputra river using different densities. J. Sylhet. Agril. Univ., 1(2):265–271.
6. Alvarado F, Robinson JW. 1979. A kinetic study of the interactions between amino acids and monosaccharides at the intestinal brush‐border membrane. J. Phys. 295 (1):457-475.
7. Alvarado F. 1966. Transport of sugars and amino acids in the intestine: evidence for common carrier. Science. 151(37):1010-1013. doi: 10.1126/science.151.3713.1010
8. Anderson J, Jackson AJ, Matty AJ, Capper BS. 1984. Effects of dietary carbohydrate and fibre on the tilapia Oreochromis niloticus (Linn.). Aquaculture. 37 (4):303-314. doi: 10.1016/0044-8486(84)90296-5

9. Andrews SR, Sahu NP, Pal AK, Mukherjee SC, Kumar S. 2011. Yeast extract, brewer’s yeast and spirulina in diets for Labeo rohita fingerlings affect haemato-immunological responses and survival following Aeromonas hydrophila challenge. Res. Vet. Sci. 91 (1):103-109. doi: 10.1016/j.rvsc.2010.08.009
10. Austreng E, Risa S, Edwards DJ, Hvidsten H. 1977. Carbohydrate in rainbow trout diets. II. Influence of carbohydrate levels on chemical composition and feed in different families. Aquaculture.11(1):39-50. doi: 10.1016/0044-8486(77)90152-1
11. Bailey JS, Blankenship LC, Cox NA. 1991. Effect of fructooligosaccharide on Salmonella colonization of the chicken intestine. Poult. Sci.70 (12):2433-2438.
12. Bhatnagar A, Devi P. 2013. Water quality guidelines for the management of pond fish culture. Intl. J. Env. Sci. 3(6):1980–2009. doi: 10.6088/ijes.2013030600019
13. Bhatnagar A, Singh G. 2010. Culture fisheries in village ponds: a multi-location study in Haryana, India. Agric. Biol. J. N. Am., 2010, 1(5): 961-968 doi: 10.5251/abjna.2010.1.5.961.968
14. Biswas G, Korenaga H, Takayama H, Kono T, Shimokawa H, Sakai M. 2012. Cytokine responses in the common carp Cyprinus carpio L. treated with baker's yeast extract. Aquaculture. 356:169-175. doi: 10.1016/j.aquaculture.2012.05.019
15. Bongers A, van den Heuvel EG. 2003. Prebiotics and the bioavailability of minerals and trace elements. Food. Rev. Intl. 19 (4):397-422. doi: 10.1081/FRI-120025482
16. Bryan R, Soderberg W, Blanchet H, Sharpe EW. 2011. Management of FishPonds in Pennsylvania. Management of Fishponds in Pennsylvania. Retrieved 5th July 2020 from https://extension.psu.edu/management-of-fish-ponds-in-pennsylvania
17. Burel C, Person‐Le Ruyet J, Gaumet F, Le Roux A, Severe A, Boeuf G. 1996. Effects of temperature on growth and metabolism in juvenile turbot. J. Fish. Biol. 49 (4):678-692. doi: 10.1111/j.1095-8649.1996.tb00064.x
18. Chen CY, Wooster GA, Bowser PR. 2004. Comparative blood chemistry, histopathology of tilapia infected with Vibrio vulnificus or Streptococcus iniae exposed to carbon tetrachloride, gentamicin, or copper sulfate. Aquaculture 239 (1-4):421-443.
19. Crane B. 2006. Results of water quality measurements in Messer pond. Water Sampling Summary pp 1-9.
20. Das B, Sarker B, Hossain A, Alam M, Iqbal MM. 2019. Optimization of 17α-methyltestosterone dose to produce quality mono-sex Nile tilapia (Oreochromis niloticus). 1st International Conference on Sustainable Fisheries. Sylhet Agricultural University, Sylhet-3100, Bangladesh, p. 67.
21. Dawood MA, Eweedah NM, Moustafa EM, Shahin MG. 2020. Synbiotic effects of Aspergillus oryzae and β-glucan on growth and oxidative and immune responses of nile Tilapia, Oreochromis niloticus. Prob. Anti. Prot. 12 (1): 172-183.
22. Dawood MA, Koshio S, Esteban MA. 2018. Beneficial roles of feed additives as immunostimulants in aquaculture: a review. Rev. Aqua. 10 (4):950-974.
23. Denev S, Staykov Y, Moutafchieva R, Beev G. 2009. Microbial ecology of the gastrointestinal tract of fish and the potential application of probiotics and prebiotics in finfish aquaculture. Intl. Aqua. Res. 1 (1) 1-29.
24. El‐Haroun ER, Goda AS, Kabir Chowdhury MA. 2006. Effect of dietary probiotic Biogen® supplementation as a growth promoter on growth performance and feed utilization of Nile tilapia Oreochromis niloticus (L.). Aqua. Res. 37 (14):1473-1480.
25. Enes P, Panserat S, Kaushik S, Oliva-Teles AA. 2008. Hepatic glucokinase and glucose-6-phosphatase responses to dietary glucose and starch in gilthead sea bream (Sparus aurata) juveniles reared at two temperatures. Mol. Integ. Phy. 149 (1):80-86.
26. Francis G, Makkar HP, Becker K. 2005. Quillaja saponins- a natural growth-promoter for fish. Ani. Feed. Sci. Tec.121(1-2):147-157. doi: 10.1016/j.anifeedsci.2005.02.015
27. Giardina B, Antonini E, Brunori M. 1973. Hemoglobin in fishes: Structural and functional properties of trout hemoglobins. Neth. J. Sea Res., 7:339–344. doi: 10.1016/0077-7579(73)90056-2.
28. Goda AMA, Mabrouk HAHH, Wafa MAEH, El-Afifi TM. 2012. Effect of using baker's yeast and exogenous digestive enzymes as growth promoters on growth, feed utilization and hematological indices of Nile tilapia, Oreochromis niloticus fingerlings. J. Agri. Sci. Tec. B 2:15-28
29. Goran SMA, Omar SS, Anwer AY. 2017. Assessment of yeast as a dietary additive on haematology and water quality of common carp in a recirculating aquaculture system. In AIP Conference Proceedings, 1888(1):020023. doi: 10.1063/1.5004300
30. Hasan R, Hossaim MA, Islam MR, Iqbal MM. 2021. Does commercial probiotics improve the growth performance and hematological parameters of tilapia, Oreochromis niloticus? Aqua. Res.4(2): XX (in production).
31. Hassaan MS, Soltan MA, Ghonemy MMR. 2014. Effect of synbiotics between Bacillus licheniformis and yeast extract on growth, hematological and biochemical indices of the Nile tilapia (Oreochromis niloticus). Egypt. Aqua. Res. 40(2) 199-208.
32. Hokazono S, Tanaka Y, Katayama T, Chichester CO, Simpson KL. 1979. Intestinal transport of L-lysine in rainbow trout, Salmo gairdneri. Bull. Jpn. Sot. Sci. Fish 45 (7):845-848.
33. Hoseinifar SH, Mirvaghefi A, Amoozegar MA, Sharifian M, Esteban MÁ. 2015. Modulation of innate immune response, mucosal parameters and disease resistance in rainbow trout (Oncorhynchus mykiss) upon synbiotic feeding. Fish Shellfish Immunol. 45(1):27-32. doi: 10.1016/j.fsi.2015.03.029
34. Hoseinifar SH, Mirvaghefi A, Merrifield DL. 2011a.: The effects of dietary inactive brewer's yeast Saccharomyces cerevisiae var. ellipsoideus on the growth, physiological responses and gut microbiota of juvenile beluga (Huso huso). Aquaculture 318 (1-2):90-94. doi: 10.1016/j.aquaculture.2011.04.043
35. Hoseinifar SH, Mirvaghefi A, Merrifield DL, Amiri BM, Yelghi S, Bastami KD. 2011b. The study of some haematological and serum biochemical parameters of juvenile beluga (Huso huso) fed oligofructose. Fish. Phy. Bioc .37(1) 91-96.
36. Hoseinifar SH, Mirvaghefi A, Mojazi Amiri B, Rostami HK, Merrifield DL. 2011c. The effects of oligofructose on growth performance, survival and autochthonous intestinal microbiota of beluga (Huso huso) juveniles. Aqua. Nutr. 17(5):498-504.
37. Hossain MA, Akter M, Iqbal MM. 2017. Diversity of Fish Fauna in Kusiara River (Fenchungonj Upazilla), Northeast Bangladesh. J. Aqua. Trop. 32(1):1–13.
38. Htun-Han M. 1978. The reproductive biology of the dab Limanda limanda (L.) in the North Sea: Seasonal changes in the ovary. J. Fish Bio. 13(3):351–359. doi: 10.1111/j.1095-8649.1978.tb03443.x
39. Hung SS, Storebakken T. 1994. Carbohydrate utilization by rainbow trout is affected by feeding strategy. J. Nutr.124(2):223-230. doi: 10.1093/jn/124.2.223
40. Ibrahem MD, Fathi M, Mesalhy S, Abd El-Aty AM. 2010. Effect of dietary supplementation of inulin and vitamin C on the growth, hematology, innate immunity, and resistance of Nile tilapia (Oreochromis niloticus). Fish Shellfish Immunol. 29(2):241-246.
41. Kausar R, Salim M. 2006. Effect of water temperature on the growth performance and feed conversion ratio of Labeo rohita. J. Pak. Vet. 26(3):105-108.
42. Lee SM, Kim KD, Lall SP. 2003. Utilization of glucose, maltose, dextrin and cellulose by juvenile flounder (Paralichthys olivaceus). Aquaculture. 221(1-4) : 427-438.
43. Lee SM, Lee JH. 2004. Effect of dietary glucose, dextrin and starch on growth and body composition of juvenile starry flounder Platichthys stellatus. Fish. Sci. 70(1) 53-58.
44. Li P, Gatlin DM. 2005. Evaluation of the prebiotic GroBiotic®-A and brewer’s yeast as dietary supplements for sub-adult hybrid striped bass (Morone chrysops× M. saxatilis) challenged in situ with Mycobacterium marinum. Aquaculture 248 (1-4):197-205. doi: 10.1016/j.aquaculture.2005.03.005
45. Mansour MR, Akrami R, Ghobadi SH, Denji KA, Ezatrahimi N, Gharaei A. 2012. Effect of dietary mannan oligosaccharide (MOS) on growth performance, survival, body composition, and some hematological parameters in giant sturgeon juvenile (Huso huso Linnaeus, 1754). Fish. Phy. Bioc. 38(3):829-835.
46. McLean EWEN, Craig SR. 2006. Nutrigenomics in aquaculture research: a key in the Aquanomic revolution. In Nutritional biotechnology in the feed and food industries: Proceedings of Alltech's 22nd Annual Symposium, Lexington, Kentucky, USA, Alltech UK 23-26 April 2006 p.433-444.
47. Montajami S, Hajiahmadyan M, Forouhar Vajargah M, Hosseini Zarandeh AS, Shirood Mirzaie F, Hosseini SA. 2012. Effect of symbiotic (Biomin imbo) on growth performance and survival rate of Texas cichlid (Herichthys cyanoguttatus) larvae. Glob. Vet. 9(3):358-61.
48. Munkittrick KR, Leatherland JF. 1983. Haematocrit values in feral goldfish, Camssius auratus L., as indicators of the health of the population. J.Fish Bio. 23(2):153-161. doi: 10.1111/j.1095-8649.1983.tb02890.x
49. Nekoubin H, Gharedashi E, Imanpour MR, Asgharimoghadam A. 2012. The influence of synbiotic (Biomin Imbo) on growth factors and survival rate of Zebrafish (Danio rerio) larvae via supplementation with biomar. Glob. Vet. 8(5): 503-506.
50. Ngugi CC, James RB, Bethuel OO. 2007. A New Guide to Fish Farming in Kenya, Oregon State University, USA. https://agris.fao.org/agris-search/search.do?recordID=XF2015022349 (08.08.2020)
51. Oliva-Teles A, Gonçalves P. 2001. Partial replacement of fishmeal by brewers yeast (Saccaromyces cerevisae) in diets for sea bass (Dicentrarchus labrax) juveniles. Aquaculture, 202(3-4):269-278. doi: 10.1016/S00448486(01)00777-3
52. Olvera-Novoa MA, Campos SG, Sabido MG, Martínez Palacios CA. 1990. The use of alfalfa leaf protein concentrates as a protein source in diets for tilapia (Oreochromis mossambicus).Aquaculture, 90(3): 291–302. doi: 10.1016/0044-8486(90)90253-J
53. Ozório RO, Portz L, Borghesi R, Cyrino JE. 2012. Effects of dietary yeast (Saccharomyces cerevisia) supplementation in practical diets of tilapia (Oreochromis niloticus). Animals, 2(1):16-24. doi: 10.3390/ani2010016
54. Panase P, Mengumphan K. 2015. Growth performance, length-weight relationship and condition factor of backcross and reciprocal hybrid catfish reared in net cages. Intl. J. Zoo. Res. 11(2):57–64. doi: 10.3923/ijzr.2015.57.64
55. Pandiyan P, Balaraman D, Thirunavukkarasu R, George EGJ, Subaramaniyan K, Manikkam S, Sadayappan B 2013. Probiotics in aquaculture. Drug. Inv. Tod. 5(1):55-59. doi: 10.1016/j.dit.2013.03.003
56. Panigrahi A, Kiron V, Puangkaew J, Kobayashi T, Satoh S, Sugita H. 2005. The viability of probiotic bacteria as a factor influencing the immune response in rainbow trout Oncorhynchus mykiss. Aquaculture. 243(1-4):241-254. doi: 10.1016/j.aquaculture.2004.09.032
57. Pechsiri J, Yakupitiyage A. 2005. A comparative study of growth and feed utilization efficiency of sex-reversed diploid and triploid Nile tilapia, Oreochromis niloticus L. Aqua. Res. 13(1):45–51. doi: 10.1111/j.1365-2109.2004.01182.x
58. Phillips Jr AM, Tunison AV, Brockway DR. 1948. The utilization of carbohydrates bytrout. NY Conserv. Dep. Fish Res. Bull. 11:34-43.
59. Reza A, Abdolmajid H, Abbas M, Abdolmohammad AK. 2009. Effect of dietary prebiotic inulin on growth performance, intestinal microflora, body composition and hematological parameters of juvenile beluga, Huso huso. J. World. Aqua. Soc. 40(6):771-779. doi: 10.1111/j.1749-7345.2009.00297.x
60. Riggs A. 1970. Properties of Fish Hemoglobins. In W. S. Hoar & D. J. B. T.-F. P. Randall (Eds.), The Nervous System, Circulation, and Respiration. 4:209–252). Academic Press. doi: 10.1016/S1546-5098(08)60131-4
61. Rumsey GL, Kinsella JE, Shetty KJ, Hughes SG. 1991. Effect of high dietary concentrations of brewer's dried yeast on growth performance and liver uricase in rainbow trout (Oncorhynchus mykiss). Ani. Feed. Sci. Tec. 33(3-4):177 183.
62. Santhosh SB. 2017. Guidelines for water quality management for fish culture in Tripura. ICAR Research Complex for NEH Region, Tripura Centre, India, 27.
63. Shiau SY, Chuang JC. 1995. Utilization of disaccharides by juvenile tilapia, Oreochromis niloticus x O. aureus. Aquaculture. 133(3-4):249-256. doi: 10.1007/s10695-009-9366-y
64. Smiricky-Tjardes MR, Grieshop CM, Flickinger EA, Bauer LL, Fahey JGC. 2003. Dietary galactooligosaccharides affect ileal and total-tract nutrient digestibility, ileal and fecal bacterial concentrations, and ideal fermentative characteristic of growing pigs. J. Ani. Sci. 81(10):2535-2545. doi: 10.2527/2003.81102535x
65. Ta’ati R, Soltani M, Bahmani M, Zamini AA. 2011. Growth performance, carcass composition and immune physiological indices in juvenile great sturgeon (Huso huso) fed on commercial prebiotic. Iran. J. Fish. Sci. 10:324–335.
66. Tan Q, Xie S, Zhu X, Lei W, Yang AY. 2006. Effect of dietary carbohydrate sources on growth performance and utilization for gibel carp (Carassius auratus) and Chinese long snout catfish (Leiocassis longirostris). Aqua. Nutr. 12(1):61-70. doi: 10.1111/j.1365-2095.2006.00382.x
67. Welker TL, Lim C. 2011. Use of probiotics in diets of tilapia. Aqu. Res. Dev. S1 doi: 10.4172/2155-9546.S1-014
68. Wörle-Knirsch JM, Kern K, Schleh C, Adelhelm C, Feldmann C, Krug HF. 2007. Nanoparticulate vanadium oxide potentiated vanadium toxicity in human lung cells. Env. Sci. Tec. 41(1):331-336. doi: 10.1021/es061140x
69. Ye JD, Wang K, Li FD, Sun YZ. 2011. Single or combined effects of fructo- and mannan oligosaccharide supplements and Bacillus clausii on the growth, feed utilization, body composition, digestive enzyme activity, innate immune response and lipid metabolism of the Japanese flounder. Aqua. Nutr. 17(4):902–911. doi: 10.1111/j.1365-2095.2011.00863.x
70. Zaid AA, Olasunkanmi AO, Ajoke BR. 2013. Inclusion effect of graded levels of molases in the diet of Clarias gariepinus juvenile. Intl. Fish. Aqu. 5(7):172-176.
71. Zhou M, Liang R, Mo J, Yang S, Gu N, Wu Z, Sarath Babu V, Li Jun, Yunmao H Lin L. 2018. Effects of brewer's yeast hydrolysate on the growth performance and the intestinal bacterial diversity of largemouth bass (Micropterus salmoides). Aquaculture 484:139-144. doi: 10.1016/j.aquaculture.2017.11.006