Rosa canina özütü kullanılarak gümüş nanopartiküllerin biyosentezi ve insan kolon adenokarsinoma hücre hattı HT29 üzerindeki anti-kanser ve anti-metastatik aktivitesi

Abstract

Kolon kanseri, geleneksel tedavi yöntemlerinde kaydedilen ilerlemelere rağmen, dünyada kansere bağlı ölüm nedenleri arasında dördüncü sırada yer almaktadır. Bu nedenle, kolon kanserinin tedavisi için daha etkili yeni tedavi stratejilerine ihtiyaç duyulmaktadır. Bitki özleri kullanılarak sentezlenen gümüş nanopartiküller (AgNP’ler), terapötik uygulamalar göstermiş ve bu durum onları iyi bir kanser önleyici ajan adayı haline getirmiştir. Bu çalışmada, kuşburnu özütü ile sentezlenmiş gümüş nanopartiküllerin insan kolon adenokarsinoma hücre hattı HT29 üzerindeki anti-metastatik ve anti-kanser aktivitesinin değerlendirilmesi amaçlanmıştır. Gümüş nanopartiküllerin biyolojik sentezi, kuşburnu bitkisinin sulu özütü kullanılarak gerçekleştirildi. R-AgNP'ler, UV-görünür spektrofotometre ve taramalı elektron mikroskobu (SEM) gibi çeşitli analitik tekniklerle karakterize edildi. HT29 hücreleri 48 saat süresince çeşitli konsantrasyonlarda R-AgNP'lerle (0-20 µg/mL) muamele edildi. Sentezlenen R-AgNP'lerin insan kolon kanseri hücrelerine karşı sitotoksik aktivitesi MTT assay ile değerlendirildi ve IC50 değerinin 48 saatlik inkübasyon sonucunda 7,89 µg/mL olduğu belirlendi. R-AgNP'lerin anti-metastatik potansiyeli, HT29 hücreleri üzerinde scratch assay kullanılarak çalışıldı. R-AgNP'lerin doza bağımlı olarak hücre hareketliliğinde önemli bir azalmaya neden olduğu belirlendi. Sonuç olarak, bu bulgular biyosentezlenen gümüş nanopartiküllerin insan kolon kanseri tedavisi için umut verici yeni terapötik ajanlar olabileceğini göstermektedir.

Keywords

• Gümüş nanopartiküller,Kolon kanseri,Metastaz,AgNP

Biosynthesis of silver nanoparticles using Rosa canina extract and its anti-cancer and anti-metastatic activity on human colon adenocarcinoma cell line HT29

Abstract

Despite progress in conventional treatment methods for colon cancer, it remains the fourth leading cause of cancer-related deaths in the world. Therefore, more effective new treatment strategies for colon cancer are needed. Silver nanoparticles (AgNPs) synthesized using plant extracts have shown therapeutic applications and make it to be a good anti-cancer candidates. The aim of this study was to evaluate the anti-metastatic and anti-cancer activity of biosynthesized silver nanoparticles from Rosa canina extract on the human colon adenocarcinoma cell line HT29. The biosynthesis of AgNPs was carried out using Rosa canina extract. R-AgNPs were characterized by techniques such as UV-vis spectrophotometer and scaining electron microscopy (SEM). HT29 cells were incubated with different concentrations of AgNPs (0-20 µg/mL) for 48 h. The cytotoxic activity of the synthesized R-AgNPs against human colon adenocarcinoma cells HT29 was investigated by MTT assay and the IC50 value were found to be 7,89 µg/mL at 48 h incubation. Anti-metastatic potential of R-AgNPs were determined on HT29 cells using scratch assay. R-AgNPs induced a significant decrease of cell motility in dose-dependent manner. In conlusion, these findings suggest that the biosynthesized AgNPs may be promising new therapeutic agents for the treatment of human colon cancer.

Keywords

• Silver nanoparticles,Colon cancer,Metastasis,AgNP

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