Effect of synthetic cannabinoids (JWH-018) on antibody response to HBV vaccination

Abstract

Synthetic cannabinoids can affect the immune system and can cause some changes in immune response. The immune response to the Hepatit B vaccine is complex. Studies on hepatitis B vaccine antibody response and JWH-018 are extremely limited. The main aim of this study was to investigate the effect of JWH-018 on anti-HBs Ag changes before or after Hepatitis B vaccination. The study was performed on C57BL6 mice (n=25). Mice were divided into 3 groups. Control Group; it was immunized Engerix B at 3 times 3-week intervals. Group 1; JWH-018 (1 mg/kg) was administered once a week for 4 weeks. At the end of this period, Engerix B was immunized 3 times at 3-week intervals. Group 2; it was immunized 3 times at 3-weeks intervals with Engerix B. At the end of this period JWH-018 (1 mg/kg) was treated once a week for 4 weeks. Blood samples (3 times with an interval of 2 weeks) were collected at the end of drug and vaccine administration. It was calculated that the means obtained from the control group were lower than the other groups. The final measurement of the within-group was higher than other measures and was statistically significant (p=0,017). Statistical difference was measured in the first (p=0,018) and third measurements (p=0,005) of the between groups. A total of 5 mice from the experimental groups died at different stages of the study. In this study, the use of JWH-018 has been shown to be effective on the anti-HBs parameter. We think that our study is very valuable in terms of proving the relationship between JWH-018 and Anti-Hbs parameter. However, more data are needed to understand causation.

Keywords

• Hepatitis B
• Immunity
• JWH-018
• Mice
• Vaccination

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