Sığla Yağının Paslanmaz Çelik Yüzeyde Staphylococcus aureus Üzerine Antibakteriyel Aktivitelerinin Değerlendirilmesi

Abstract

Sığla ağacı (Liquidambar orientalis Miller) endemik bir tür olup özünden elde edilen sığla yağının antiparaziter ve antibakteriyel özellikleri bulunmaktadır. Staphylococcus aureus önemli bir mastitis etkenidir. Süt ve süt toplama tank yüzeylerinde bulunması ve salgıladığı toksinler gıda zehirlenmeleri oluşturabilir. Bu çalışmada; deneysel olarak paslanmaz çelik yüzeye uygulanan bakteri kültürü üzerine farklı sürelerde uygulanan sığla yağı içerikli solüsyonun etkilerinin araştırılması amaçlanmıştır. Çalışma sonucunda sığla yağının %5, %10, %15 ve %20 konsantrasyonlarda süt toplama tankı yüzeyinde tüm konsantrasyonlarda S. aureus üzerinde etkili olduğu tespit edilmiştir. Sığla yağının S. aureus varlığı üzerinde 7,94 log cfu/ml inaktivasyon gerçekleştirdiği belirlenmiştir. Sığla yağı endüstriyel anlamda yüzey temizliğinde doğal bir antimikrobiyal ajan olarak kullanılabilir. Bu çalışma sığla yağının dezenfeksiyon özelliklerinin belirlenmesi bakımından ilk çalışma özelliğindedir.

Keywords

• Sığla ağacı
• Sığla yağı
• Staphylococcus aureus
• Süt

Project Number

1919B011803682

Evaluation of Antibacterial Activities of Stryax Liquidus on Staphylococcus aureus on Stainless Steel Surface

Abstract

Sığla tree (Liquidambar orientalis Miller) is an endemic species and the styrax liquidus obtained from its extract has antiparasitic and antibacterial properties. Staphylococcus aureus is an important agent of mastitis, it can be found in milk and on the milk collection tank surfaces and the toxins it secretes may cause food poisoning. In this study; It was aimed to investigate the effects of the styrax liquidus solution applied for different periods on the bacterial culture applied to the stainless-steel surface experimentally. As a result of the study, it was determined that styrax liquidus was effective on S. aureus in all concentrations on the surface of the bulk tank milk at 5%, 10%, 15% and 20% concentrations. It was determined that styrax liquidus was reduced population of S. aureus level of 7.94 log cfu/ml. Styrax liquidus could be used as a natural antimicrobial agent in industrial surface cleaning. This is the first study to determine the disinfection properties of styrax liquidus.

Keywords

• Styrax liquidus
• Sığla tree
• Staphylococcus aureus
• Milk

Supporting Institution

TÜBİTAK 2209-A 2018/2 University Students Research Projects Support Program

Project Number

1919B011803682

References

1. Arslan, M.B., Şahin, H.T., 2016. Unutulan Bir Orman Ürünü Kaynağı: Anadolu Sığla Ağacı (Liquidambar Orientalis Miller). Journal Of Bartin Faculty Of Forestry 18(1), 103-117.
2. Aydıngöz, M., Bulut, S., 2014. Egenin Gizli Kalmış Şifa İksiri: Sığla. Afyon Kocatepe University Journal of Science & Engineering 14(1), 1-6.
3. Bayramoğlu, E.E., 2010. Soaking with Storax- Possibility of Using Siĝla Tree (Liquidambar orientalis Mill. Var orientalis) Storax as Bactericide in the Soaking Float. Jalca. Journal of the American Leather Chemists Association 105, 62-68.
4. Cabarkapa, I., Colovic, R., Duragic, O., Popovic, S., Kokic, B., Milanov, D., Pezo, L., 2019. Anti-biofilm activities of essential oils rich in carvacrol and thymol against Salmonella Enteritidis. Biofouling 35 (3), 361-375.
5. Chmielewski, R.A.N., Frank, J.F., 2003. Biofilm formation and control in food processing facilities. Comprehensive Reviews in Food Science and Food Safety 2, 22–32.
6. Dhanashekar, R., Akkinepalli, S., Nellutla, A., 2012. Milk-borne infections. An analysis of their potential effect on the milk industry. Germs 2(3), 101.
7. Ding, T., Yu, Y.Y., Schaffner, D.W., Chen, S.G., Ye, X.Q., Liu, D.H., 2016. Farm to consumption risk assessment for Staphylococcus aureus and staphylococcal enterotoxins in fluid milk in China. Food Control 59, 636-643.
8. Engel, J.B., Heckler, C., Tondo, E.C., Daroit, D.J., da Silva Malheiros, P., 2017. Antimicrobial activity of free and liposome-encapsulated thymol and carvacrol against Salmonella and Staphylococcus aureus adhered to stainless steel. International Journal of Food Microbiology 252, 18-23.

9. Gootz, T.D., 2010. The global problem of antibiotic resistance. Critical Reviews in Immunology, 30(1), 79-93.
10. Hamadi, F., Asserne, F., Elabed, S., Bensouda, S., Mabrouki, M., Latrache, H., 2014. Adhesion of Staphylococcus aureus on stainless steel treated with three types of milk. Food Control 38, 104-108.
11. Hamadi, F., Latrache, H., Mabrrouki, M., Elchmari, A., Outzourhit, A., Ellouali, M., 2005. Effect of pH on distribution and adhesion of Staphylococcus aureus to glass. Journal of Adhesion and Science of Technology 19, 73-85.
12. Haug, A., Høstmark, A.T., Harstad, O.M., 2007. Bovine milk in human nutrition–a review. Lipids in Health and disease 6(1), 1-16.
13. International Standart Office, 6888-1-ISO, 2003. Microbiology of food and animal feedingstuffs – Horizontal method for the enumeration of coagulasepositive staphylococci (Staphylococcus aureus and other species).
14. Kandpal, S.D., Srivastava, A.K., Negi, K.S., 2012. Estimation of quality of raw milk (open & branded) by milk adulteration testing kit. Indian Journal of Community Health 24(3), 188-192.
15. Keyvan, E., Tutun, H., 2019. Effects of Carvacrol on Staphylococcus aureus isolated from bulk tank milk. Medycyna Weterynaryjna 75(04), 238-241.
16. Keyvan, E., Yurdakul, O., Sen, E., 2020. Staphylococcal Enterotoxins and Enterotoxigenic Staphylococcus aureus in Raw Milk: A Screening Study. Kocatepe Veteriner Dergisi 13(2), 104-109.
17. Keyvan, E., Yurdakul, O., Kocasarı, F., Tutun, H., Demirtaş, A., Kahraman, H.A., Sen, E., 2018. Detection of ochratoxin A in bulk tank milk. Kocatepe Veteriner Dergisi, 11(3), 255-259.
18. Kusumaningrum, H.D., Riboldi, G., Hazeleger, W.C., Beumer, R.R., 2003. Survival of foodborne pathogens on stainless steel surfaces and cross-contamination to foods. International Journal of Food Microbiology 85(3), 227-236.
19. Le Loir, Y., Baron, F., Gautier, M., 2003. Staphylococcus aureus and food poisoning. Genetics and Molecular Research 2(1), 63-76.
20. Marques, S.C., Rezende, J.D.G.O.S., Aparecida de Freitas Alves, L., Silva, B.C., Alves, E., Ronaldo de Abreu, L., 2007. Formation of biofilms by Staphylococcus aureus on stainless steel and glass surface and its resistance to some selected chemical sanitizers. Brazilian Journal of Microbiology 38, 538-543.
21. Nathan, C., Cars, O., 2014. Antibiotic resistance--problems, progress, and prospects. New England Journal of Medicine 371 (19), 1761-1763.
22. Normanno, G., Firinu, A., Virgilio, S., Mula, G., Dambrosio, A., Poggiu, A., Celano, G. V., 2005. Coagulase-positive Staphylococci and Staphylococcus aureus in food products marketed in Italy. International Journal of Food Microbiology 98(1), 73-79.
23. Okmen, G., Turkcan, O., Ceylan, O., Gork, G., 2014. The Antimicrobial Activity of Aliquidambar orientalis mill. Against Food Pathogens and Antioxidant Capacity of Leaf Extracts. African Journal of Traditional, Complementary and Alternative Medicines 11(5), 28-32.
24. Oliver, S.P., Jayarao, B.M., Almeida, R.A., 2005. Foodborne pathogens in milk and the dairy farm environment: food safety and public health implications. Foodborne Pathogens & Disease 2(2), 115-129.
25. Oulahal, N., Brice, W., Martial, A., Degraeve, P., 2008. Quantitative analysis of survival of Staphylococcus aureus or Listeria innocua on two types of surfaces: polypropylene and stainless steel in contact with three different dairy products. Food Control 19, 178-185.
26. Painter, J.A., Hoekstra, R.M., Ayers, T., Tauxe, R.V., Braden, C.R., Angulo, F.J., Griffin, P.M., 2013. Attribution of foodborne illnesses, hospitalizations, and deaths to food commodities by using outbreak data, United States, 1998-2008. Emerging Infectious Diseases Journal 19(3), 407-415.
27. Porter JA, Morey A, Monu EA., 2020. Antimicrobial efficacy of white mustard essential oil and carvacrol against Salmonella in refrigerated ground chicken. Poultry Scicence 99 (10): 5091-5095.
28. Sağdıç, O., Özkan, G., Özcan, M., Özçelik, S., 2005. A Study on inhibitory effects of sığla tree (Liquidambar orientalis Mill. var. orientalis) storax against several bacteria. Phytotherapy Research 19(6), 549-551.
29. Turkoglu, C., Keyvan, E., 2019. Determination of aflatoxin M1 and ochratoxin A in raw, pasteurized and UHT milk in Turkey. Acta Scientiae Veterinariae 47(1), 1626.
30. Van Houdt, R., Michiels, C.W., 2010. Biofilm formation and the food industry, a focus on the bacterial outer surface. Journal of Applied Microbiology 109, 1117–1131.