Some acute phase proteins and hepcidin levels in single and dual infection with BVD and BHV-1

Year 2017, Volume: 5 Issue: 2, 115 - 123, 25.12.2017

Şima Şahinduran Mehmet Kale Reyda Kıyıcı

https://doi.org/10.24998/maeusabed.329788

Cited By: 2

Abstract

The aims of this study were to determine serum amyloid A, haptoglobin
and especially hepcidin concentrations in animals with dual  (Bovine
viral diarrhea virus / Bovine herpesvirus
-1)
and single infections ( Bovine
viral diarrhea virus or Bovine herpesvirus -1) and
reference ranges of hepcidin in healthy clinicaly cattle. Previously, studies have been conducted on only hepcidin gene
but hepcidin values has not been studied in cattle diseases.  

In this
study totaly 56 cattle were used. In 17 of them Bovine herpesvirus-1,
13 of them Bovine viral diarrhea virus,
4 of them both Bovine herpesvirus-1 and Bovine
viral diarrhea virus were diagnosed. The control group was
composed of 22 clinicaly healthy animals. According to the results animals naturally infected with dual infection did not
show more significant differences level of acute phase proteins compared to animals with single infection. Serum hepcidin values
​​did not differ between the 3 trial
groups. But there were significant differences in three trial
groups results as compared to control group (p<0.001). Haptoglobin analysis
results were similar to the
results of hepcidin analysis. We
demonstrated the normal ranges of hepcidin in 22 clinically healthy cows. According to these results, we can
suggest that the hepcidin can be used as a marker for the diagnosis of the
diseases as an acute phase protein.

Keywords

bovine viral diarrhea, bovine herpesvirus, acute phase proteins, hepcidin

BVD ve BHV-1 ile tek ve çift infeksiyonlarda bazı akut faz proteinleri ve hepcidin düzeyleri

Abstract

Bu
çalışmanın amacı, ikili (Bovine viral diarrhea virus / Bovine herpesvirus -1)
ve tek enfeksiyonlu (Bovine viral diarrhea virus veya Bovine herpesvirus -1)
hayvanlarda ve ayrıca sağlıklı sığırlarda serum amiloid A, haptoglobin ve
özellikle hepcidin konsantrasyonlarını belirlemekti. Daha
önce sadece hepcidin geni üzerinde çalışmalar yapılmış olup ancak sığır
hastalıklarında hepcidin değerleri araştırılmamıştır.

Bu
çalışmada toplam 56 sığır kullanılmıştır. Onlardan 17'inde Bovine herpesvirus-1,
13 tanesinde Bovine viral diarrhea virüsü,   bunların 4'ünde ise hem Bovine herpesvirus-1
hem Bovine viral diarrhea virüsü tespit edildi. Kontrol grubu ise 22 sağlıklı sığırdan
oluşturuldu. Bulgulara göre, çift infeksiyonlu hayvanlar ile tek
enfeksiyonlu hayvanlar arasında akut faz proteinlerinin seviyelerinde belirgin
farklılık saptanmamıştır. Serum hepcidin değerleri, 3 çalışma
grubu arasında farklılık göstermedi. Fakat üç deneme grubunda kontrol grubuna
göre anlamlı farklılıklar vardı (p <0.001). Haptoglobin
değerleri hepcidin değerlerine yakın bulundu. Bu çalışma ile ilk defa sağlıklı sığırlarda
hepcidin'in normal değerleri saptanmaya çalışıldı. Bu sonuçlara göre, hepcidin,
bir akut faz proteini olarak hastalıkların teşhisi için bir marker olarak
kullanılabileceği önerilebilir.

Keywords

bovine viral diarrhea, ; bovine herpesvirus;, akut faz proteinleri, hepcidin

References

• 1.Eckersall PD, Bell R. 2010. Acute phase proteins: Biomarkers of infection and inflammation in veterinary medicine. The veterinary Journal. 185(1): 23-27.
• 2.Eckersall PD. 2000. Recent advances and future prospects for the use of acute phase proteins as markers of disease in animals. Revue de Medicine Veterinaire.151(7): 577–584.
• 3.Genheim C, Hultén C, Carlsson U, et al. 2003. The acute phase response in calves experimentally infected with Bovine Viral Diarrhoea Virus and/or Mannheimia haemolytica. Journal of Veterinary Medicine, Series B. 50(4): 183-190.
• 4.Hamers C, Dehan P, Couvreur B, et al. 2001. Diversity among bovine pestiviruses. The Veterinary Journal. 161(2):112–122.
• 5.Jawor P, Stefaniak T. 2011. Acute Phase Proteins in Cattle. In: Acute Phase Proteins as Early Non-Specific Biomarkers of Human and Veterinary Diseases, Francisco Veas (Ed.), InTech, pp. 381-408.
• 6.Krause A, Neitz S, Magert HJ, et al. 2000. LEAP-1, a novel highly disulfide-bonded human peptide, exhibits antimicrobial activity. FEBS Lett. 480(2-3):147-150.
• 7.Lindberg ALE. 2003. Bovine Viral Diarrhoea Virus Infections and its Control. A review. Veterinary Quarterly. 25(1): 1-16.
• 8.Nemeth E, Valore EV, Territo M, et al. 2003. Hepcidin, a putative mediator of anemia of inflammation, is a type II acute-phase protein. Blood. 101(7): 2461-2463.
• 9.Park CH, Valore EV, Waring AJ et al. 2001. Hepcidin, a urinary antimicrobial peptide synthesized in the liver. The Journal of Biological Chemistry. 276 (11): 7806-7810.
• 10.Radostits OM, Gay CC, Hinchcliff KW, et al. 2008. Bovine virus diarrhea, mucosal disease. Bovine pestivirus disease complex. In: Veterinary Medicine: A Textbook of Diseases of Cattle, Horses, Sheep, Pigs, and Goats, 10th edn. Saunders Elsevier, pp.1248-1277.
• 11.Risalde MA, Molina V, Sanchez-Cordon PJ et al. 2011. Response of proinflammatory and anti-inflammatory cytokines in calves with subclinical bovine viral diarrhea challenged with bovine herpesvirus-1. Veterinary Immunology and Immunopathology. 144(1-2): 135-143.
• 12.Smith BP. 2009. Large Animal Internal Medicine. Mosby, Elsevier, St. Louis.
• 13. Srikumaran S, Kelling C, Ambagala A. 2008. Immune evasion by pathogens of bovine respiratory disease complex. Anim Health Res Rev. 8 (2): 215–229.
• 14.Swasdipan S, McGowan M, Phillips N, et al. 2002. Pathogenesis of transplacental virus infection: pestivirus replication in the placenta and fetus following respiratory infection. Microbial Pathogenesis. 32(2):49–60.
• 15.Weinstein DA, Roy CN, Fleming MD, et al. 2002. Inappropriate expression of hepcidin is associated with iron refractory anemia: implications for the anemia of chronic disease. Blood. 100 (10): 3776-3781.