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Genetic characterization of Varroa destructor (Family: Varroidae) prevalent in honeybees (Apis mellifera) in the province of Aydin in Turkey

Year 2018, Volume: 6 Issue: 1, 26 - 32, 21.07.2018
https://doi.org/10.24998/maeusabed.435383

Abstract

The aim of the present study was to identify
the haplotypes of the Varroa destructor mite which infects honeybees in
the province of Aydın in Turkey, using two different modified techniques for
the mitochondrial Cox1 gene of the mite.

In order to confirm the haplotype, two primers differing in their sequence i.e.
(ADA 01) as

forward primer 5′-TACAAAGAGGGAAGAAGCAGCC-3′ and reverse primer 5′-

GCCCCTATTCTTAATACATAGTGAAAATG-3′ and (ADA 02) with COXF primer

[5′GG(A/G)GG(A/T)GA(C/T)CC(A/T)ATT(C/T)T(A/T)TATCAAC3′] and COXRa primer
[5′GG(A/T)GACCTGT(A/TA(A/T)AATAGCAAATAC3′], were selected.

Amplified DNA 376 bp in size was acquired using (ADA 01) forward primer 5′-

TACAAAGAGGGAAGAAGCAGCC-3′ and reverse primer 5′-

GCCCCTATTCTTAATACATAGTGAAAATG-3′. SacI restriction enzyme was applied to
the amplified products; however, this restriction enzyme did not cut the DNA.

Amplified DNA, 570 bp in size was obtained using (ADA 02) COXF primer

[5’GG(A/G)GG(A/T)GA(C/T)CC(A/T)ATT(C/T)T(A/T)TATCAAC3’] COXRa and

[5’GG(A/T)GACCTGT(A/TA(A/T)AATAGCAAATAC3’]. XhoI and SacI
restriction

enzymes were used for the amplified products. Although, the SacI
restriction enzyme did not cut the DNA, the XhoI restriction enzyme cut
the amplified DNA into two fragments (bands), with the sizes of 270 and 300 bp
two bands 270 and 300 bp. While comparing the results, these bands were found
specific for Korean haplotype of V. destructor.

In conclusion, all of the 200 samples of V. destructor examined in this
study were identified to be the Korean haplotype.

References

  • Akinwande, K.L., Badejo, M.A., Ogbogu, S.S., 2012. Incidence of the Korean haplotype of Varroa destructor in southwest Nigeria. Journal of Apicultural Research 51(4), 369-370.Amdam, G.V., Hartfelder, K., Norberg, K., Hagen, A., Omholt, SW., 2004. Altered Physiology in Worker Honey Bees (Hymenoptera: Apidae) Infested with the Mite Varroa destructor (Acari: Varroidae): A Factor in Colony Loss During Overwintering?. Journal of Economic Entomology 97(3), 741-747. Anderson, D.L., Fuchs, S., 1998. Two genetically distinct populations of Varroa jacobsoni with contrasting reproductive abilities on Apis mellifera. Journal of Apicultural Research 37(2), 69-78.Anderson, D.L., Trueman, J.W.H., 2000. Varroa jacobsoni (Acari: Varroidae) is morethan one species. Experimental and Applied Acarology 24, 165-189. Ayan, A., Ural, K., Aldemir, O.S., Tutun, H., 2017a. Van Bölgesindeki Bal Arılarında (Apis mellifera) Görünen Varroa destructor’un Genetik Karakterizasyonunun Belirlenmesi Mehmet Akif Ersoy Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi 5(2), 78-84. Ayan, A., Aldemir, O.S., Selamoglu, Z., 2017b. Analysis of COI Gene Region of Varroa destructor in Honey Bees (Apis mellifera) in Province of Siirt. Turkish Journal of Veterinary Research 1(1), 20-23. Beaurepaire, A.L., Truong, T.A., Fajardo, A.C., , Dinh, T.Q., Cervancia, C., Moritz, R.F., 2015. Host Specificity in the Honeybee Parasitic Mite, Varroa spp. in Apis mellifera and Apis cerana. PLoS ONE 10(8), 1-12. Boecking, O., Genersch, E., 2008. Varroosis – the ongoing crisis in bee keeping. Journal für Verbraucherschutz und Lebensmittelsicherheit 3(2), 221-228. Chemurot, M., Akol, A.M., Masembe, C., de Smet, L., Descamps, T., de Graaf, D.C., 2016. Factors influencing the prevalence and infestation levels of Varroa destructor in honeybee colonies in two highland agro-ecological zones of Uganda. Experimental and Applied Acarology 68(4), 497-508. Dainat, B., Evans, J.D., Chen, Y.P., Gauthier, L., Neumann, P., 2012. Dead or alive: deformed wing virus and Varroa destructor reduce the life span of winter honeybees. Applied and Environmental Microbiology 78(4), 981-987. De Guzman, L., Rinderer, T.E., Stelzer, J.A., Anderson, D., 1998. Congruence of RAPD and mitochondrial DNA markers in assessing Varroa jacobsoni genotypes. Journal of Apicultural Research 37, 49-51. Dietemann, V., Nazzi, F., Martin, S.J., Anderson, D.L., Locke, B., Delaplane, K.S., Ellis, J.D., 2013. Standard methods for Varroa research. Journal of Apicultural Research 52(1), 1-54. Fazier, M., Muli, E., Conklin, T., Schmehl, D., Torto, B., Frazier, J., Tumlinson, J., Evans J.D., Raina S., 2010. A scientific note on Varroa destructor found in East Africa; threat or opportunity?. Apidologie 41, 463-465. Gajic, B., Radulovic, Z., Stevanovic, J., Kulisic, Z., Vucicevic, M., Simeunovic, P., Stanimirovicve, Z., 2013. Variability of the honey bee mite Varroa destructor in Serbia, based on mtDNA analysis. Experimental and Applied Acarology 61, 97-105. Ghazoul, J., 2005. Buzziness as usual? Questioning the global pollination crisis. Trends in Ecology & Evolution 20(7), 367-373. Jacobs, F.J., Simoens, C., DeGraaf, D.C., Deckers, J., 2006. Scope for non-wood forest products income generation from rehabilitation areas: focus on beekeeping. Journal of the Drylands 1(2),171-185. Maggi, M., Medici, S., Quintana, S., Ruffinengo, S., Marca´ngeli, J., Gimenez Martinez, P., Fuselli, S., Eguarasve, M., 2012. Genetic structure of Varroa destructor populations infesting Apis mellifera colonies in Argentina. Experimental and Applied Acarology 56, 309-318. Melin, A., Rouget, M., Midgley, J.J., Donaldson, J.S., 2014. Pollination ecosystem services in South African agricultural systems. South African Journal of Science 110(11), 1-9. Muli, E., Patch, H., Frazier, M., Frazier, J., Torto, B., Baumgarten, T., Grozinger, C., 2014. Evaluation of the distribution and impacts of parasites, pathogens, and pesticides on honey bee (Apis mellifera) populations in East Africa. PLoS One 9(4), 1-11. Muñoz, I., Garrido-Bailón, E., Martín-Hernández, R., Meana, A., Higes, M., De la Rúa, P., 2008. Genetic profile of Varroa destructor infesting Apis mellifera iberiensis colonies. Journal of Apicultural Research Bee World 47, 310-313. Navajas, M., Anderson, D.L., De Guzman, L.I., Huang, Z.Y., Clement, J., Zhou, T., Le Conte, Y., 2010. New Asian types of Varroa destructor. a potential new threat for World apiculture Apidologie 41, 181-193. Rasolofoarivao, H., Clemencet, J., Ravaomanarivo, L.H., Razafindrazaka, D., Reynaud, B., Delatte, H., 2013. Spread and strain determination of Varroa destructor (Acari: Varroidae) in Madagascar since its first report in 2010. Experimental and Applied Acarology 60, 521-530. Rosenkranz, P., Aumeier, P., Ziegelmann, B., 2010. Biology and control of Varroa destructor. Journal of Invertebrate Pathology 103, 96-119. Shen, M., Yang, X., Cox-foster, D., Cui, L., 2005. The role of Varroa mites in infections of Kashmir bee virüs (KBV) and deformed wing virus (DWV) in honey bees. Virology 342, 141-149. Solignac, M., Cornuet, J.M., Vautrin, D., Le Conte, Y., Anderson, D., Evans, J., Cros-Arteil, S., Navajas, M., 2005. The invasive Korea and Japan types of Varroa destructor, ectoparasitic mites of the Western honey bee (Apis mellifera), are two partly isolated clones Proceedings of the Royal Society of London. Series B, Biological sciences 272, 411-419. Strapazzon, R., Carneiro, F.E., Guerra Jr, J.C.V., Moretto, G., 2009. Genetic characterization of the mite Varroa destructor (Acari: Varroidae) collected from honey bees Apis mellifera (Hymenoptera, Apidae) in the State of Santa Catarina, Brazil. Genetics and Molecular Research 8(3), 990-997. Strauss, U., Human, H., Gauthier, L., Crewe, R.M., Dietemann, V., Pirk, C.W.W., 2013. Seasonal prevalence of pathogens and parasites in the savannah honeybee (Apis mellifera scutellata). Journal of Invertebrate Pathology 114, 45-52. Warrit, N., Hagen, T.A.R., Smith, D.R., Çakmak, I., 2004. Survey of Varroa destructor strains on Apis mellifera in Turkey. Journal of Apicultural Research 43(4), 190-191.
Year 2018, Volume: 6 Issue: 1, 26 - 32, 21.07.2018
https://doi.org/10.24998/maeusabed.435383

Abstract

References

  • Akinwande, K.L., Badejo, M.A., Ogbogu, S.S., 2012. Incidence of the Korean haplotype of Varroa destructor in southwest Nigeria. Journal of Apicultural Research 51(4), 369-370.Amdam, G.V., Hartfelder, K., Norberg, K., Hagen, A., Omholt, SW., 2004. Altered Physiology in Worker Honey Bees (Hymenoptera: Apidae) Infested with the Mite Varroa destructor (Acari: Varroidae): A Factor in Colony Loss During Overwintering?. Journal of Economic Entomology 97(3), 741-747. Anderson, D.L., Fuchs, S., 1998. Two genetically distinct populations of Varroa jacobsoni with contrasting reproductive abilities on Apis mellifera. Journal of Apicultural Research 37(2), 69-78.Anderson, D.L., Trueman, J.W.H., 2000. Varroa jacobsoni (Acari: Varroidae) is morethan one species. Experimental and Applied Acarology 24, 165-189. Ayan, A., Ural, K., Aldemir, O.S., Tutun, H., 2017a. Van Bölgesindeki Bal Arılarında (Apis mellifera) Görünen Varroa destructor’un Genetik Karakterizasyonunun Belirlenmesi Mehmet Akif Ersoy Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi 5(2), 78-84. Ayan, A., Aldemir, O.S., Selamoglu, Z., 2017b. Analysis of COI Gene Region of Varroa destructor in Honey Bees (Apis mellifera) in Province of Siirt. Turkish Journal of Veterinary Research 1(1), 20-23. Beaurepaire, A.L., Truong, T.A., Fajardo, A.C., , Dinh, T.Q., Cervancia, C., Moritz, R.F., 2015. Host Specificity in the Honeybee Parasitic Mite, Varroa spp. in Apis mellifera and Apis cerana. PLoS ONE 10(8), 1-12. Boecking, O., Genersch, E., 2008. Varroosis – the ongoing crisis in bee keeping. Journal für Verbraucherschutz und Lebensmittelsicherheit 3(2), 221-228. Chemurot, M., Akol, A.M., Masembe, C., de Smet, L., Descamps, T., de Graaf, D.C., 2016. Factors influencing the prevalence and infestation levels of Varroa destructor in honeybee colonies in two highland agro-ecological zones of Uganda. Experimental and Applied Acarology 68(4), 497-508. Dainat, B., Evans, J.D., Chen, Y.P., Gauthier, L., Neumann, P., 2012. Dead or alive: deformed wing virus and Varroa destructor reduce the life span of winter honeybees. Applied and Environmental Microbiology 78(4), 981-987. De Guzman, L., Rinderer, T.E., Stelzer, J.A., Anderson, D., 1998. Congruence of RAPD and mitochondrial DNA markers in assessing Varroa jacobsoni genotypes. Journal of Apicultural Research 37, 49-51. Dietemann, V., Nazzi, F., Martin, S.J., Anderson, D.L., Locke, B., Delaplane, K.S., Ellis, J.D., 2013. Standard methods for Varroa research. Journal of Apicultural Research 52(1), 1-54. Fazier, M., Muli, E., Conklin, T., Schmehl, D., Torto, B., Frazier, J., Tumlinson, J., Evans J.D., Raina S., 2010. A scientific note on Varroa destructor found in East Africa; threat or opportunity?. Apidologie 41, 463-465. Gajic, B., Radulovic, Z., Stevanovic, J., Kulisic, Z., Vucicevic, M., Simeunovic, P., Stanimirovicve, Z., 2013. Variability of the honey bee mite Varroa destructor in Serbia, based on mtDNA analysis. Experimental and Applied Acarology 61, 97-105. Ghazoul, J., 2005. Buzziness as usual? Questioning the global pollination crisis. Trends in Ecology & Evolution 20(7), 367-373. Jacobs, F.J., Simoens, C., DeGraaf, D.C., Deckers, J., 2006. Scope for non-wood forest products income generation from rehabilitation areas: focus on beekeeping. Journal of the Drylands 1(2),171-185. Maggi, M., Medici, S., Quintana, S., Ruffinengo, S., Marca´ngeli, J., Gimenez Martinez, P., Fuselli, S., Eguarasve, M., 2012. Genetic structure of Varroa destructor populations infesting Apis mellifera colonies in Argentina. Experimental and Applied Acarology 56, 309-318. Melin, A., Rouget, M., Midgley, J.J., Donaldson, J.S., 2014. Pollination ecosystem services in South African agricultural systems. South African Journal of Science 110(11), 1-9. Muli, E., Patch, H., Frazier, M., Frazier, J., Torto, B., Baumgarten, T., Grozinger, C., 2014. Evaluation of the distribution and impacts of parasites, pathogens, and pesticides on honey bee (Apis mellifera) populations in East Africa. PLoS One 9(4), 1-11. Muñoz, I., Garrido-Bailón, E., Martín-Hernández, R., Meana, A., Higes, M., De la Rúa, P., 2008. Genetic profile of Varroa destructor infesting Apis mellifera iberiensis colonies. Journal of Apicultural Research Bee World 47, 310-313. Navajas, M., Anderson, D.L., De Guzman, L.I., Huang, Z.Y., Clement, J., Zhou, T., Le Conte, Y., 2010. New Asian types of Varroa destructor. a potential new threat for World apiculture Apidologie 41, 181-193. Rasolofoarivao, H., Clemencet, J., Ravaomanarivo, L.H., Razafindrazaka, D., Reynaud, B., Delatte, H., 2013. Spread and strain determination of Varroa destructor (Acari: Varroidae) in Madagascar since its first report in 2010. Experimental and Applied Acarology 60, 521-530. Rosenkranz, P., Aumeier, P., Ziegelmann, B., 2010. Biology and control of Varroa destructor. Journal of Invertebrate Pathology 103, 96-119. Shen, M., Yang, X., Cox-foster, D., Cui, L., 2005. The role of Varroa mites in infections of Kashmir bee virüs (KBV) and deformed wing virus (DWV) in honey bees. Virology 342, 141-149. Solignac, M., Cornuet, J.M., Vautrin, D., Le Conte, Y., Anderson, D., Evans, J., Cros-Arteil, S., Navajas, M., 2005. The invasive Korea and Japan types of Varroa destructor, ectoparasitic mites of the Western honey bee (Apis mellifera), are two partly isolated clones Proceedings of the Royal Society of London. Series B, Biological sciences 272, 411-419. Strapazzon, R., Carneiro, F.E., Guerra Jr, J.C.V., Moretto, G., 2009. Genetic characterization of the mite Varroa destructor (Acari: Varroidae) collected from honey bees Apis mellifera (Hymenoptera, Apidae) in the State of Santa Catarina, Brazil. Genetics and Molecular Research 8(3), 990-997. Strauss, U., Human, H., Gauthier, L., Crewe, R.M., Dietemann, V., Pirk, C.W.W., 2013. Seasonal prevalence of pathogens and parasites in the savannah honeybee (Apis mellifera scutellata). Journal of Invertebrate Pathology 114, 45-52. Warrit, N., Hagen, T.A.R., Smith, D.R., Çakmak, I., 2004. Survey of Varroa destructor strains on Apis mellifera in Turkey. Journal of Apicultural Research 43(4), 190-191.
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Details

Primary Language English
Subjects Health Care Administration
Journal Section Research Article
Authors

Adnan Ayan

Osman Selçuk Aldemir This is me

Publication Date July 21, 2018
Submission Date June 21, 2018
Published in Issue Year 2018 Volume: 6 Issue: 1

Cite

APA Ayan, A., & Aldemir, O. S. (2018). Genetic characterization of Varroa destructor (Family: Varroidae) prevalent in honeybees (Apis mellifera) in the province of Aydin in Turkey. Mehmet Akif Ersoy University Journal of Health Sciences Institute, 6(1), 26-32. https://doi.org/10.24998/maeusabed.435383
AMA Ayan A, Aldemir OS. Genetic characterization of Varroa destructor (Family: Varroidae) prevalent in honeybees (Apis mellifera) in the province of Aydin in Turkey. Mehmet Akif Ersoy University Journal of Health Sciences Institute. July 2018;6(1):26-32. doi:10.24998/maeusabed.435383
Chicago Ayan, Adnan, and Osman Selçuk Aldemir. “Genetic Characterization of Varroa Destructor (Family: Varroidae) Prevalent in Honeybees (Apis Mellifera) in the Province of Aydin in Turkey”. Mehmet Akif Ersoy University Journal of Health Sciences Institute 6, no. 1 (July 2018): 26-32. https://doi.org/10.24998/maeusabed.435383.
EndNote Ayan A, Aldemir OS (July 1, 2018) Genetic characterization of Varroa destructor (Family: Varroidae) prevalent in honeybees (Apis mellifera) in the province of Aydin in Turkey. Mehmet Akif Ersoy University Journal of Health Sciences Institute 6 1 26–32.
IEEE A. Ayan and O. S. Aldemir, “Genetic characterization of Varroa destructor (Family: Varroidae) prevalent in honeybees (Apis mellifera) in the province of Aydin in Turkey”, Mehmet Akif Ersoy University Journal of Health Sciences Institute, vol. 6, no. 1, pp. 26–32, 2018, doi: 10.24998/maeusabed.435383.
ISNAD Ayan, Adnan - Aldemir, Osman Selçuk. “Genetic Characterization of Varroa Destructor (Family: Varroidae) Prevalent in Honeybees (Apis Mellifera) in the Province of Aydin in Turkey”. Mehmet Akif Ersoy University Journal of Health Sciences Institute 6/1 (July 2018), 26-32. https://doi.org/10.24998/maeusabed.435383.
JAMA Ayan A, Aldemir OS. Genetic characterization of Varroa destructor (Family: Varroidae) prevalent in honeybees (Apis mellifera) in the province of Aydin in Turkey. Mehmet Akif Ersoy University Journal of Health Sciences Institute. 2018;6:26–32.
MLA Ayan, Adnan and Osman Selçuk Aldemir. “Genetic Characterization of Varroa Destructor (Family: Varroidae) Prevalent in Honeybees (Apis Mellifera) in the Province of Aydin in Turkey”. Mehmet Akif Ersoy University Journal of Health Sciences Institute, vol. 6, no. 1, 2018, pp. 26-32, doi:10.24998/maeusabed.435383.
Vancouver Ayan A, Aldemir OS. Genetic characterization of Varroa destructor (Family: Varroidae) prevalent in honeybees (Apis mellifera) in the province of Aydin in Turkey. Mehmet Akif Ersoy University Journal of Health Sciences Institute. 2018;6(1):26-32.