The effect of Lysimachia savranii on the breast canser cells in cell culture conditions compared to normal cells

Year 2019, Volume: 7 Issue: 2, 68 - 75, 30.12.2019

İşil Aydemir Caner Özbey Gonca Dönmez Arat Oktay Özkan Ahmet Savran Sevinç İnan Mehmet İbrahim Tuğlu

https://doi.org/10.24998/maeusabed.631012

Cited By: 1

Abstract

The
breast cancer has become a threat to women's lives at an increasing rate. The
inadequate treatments of surgical procedures and chemotherapy affect negatively
quality of patient life. Therefore, there has been an increase in demand for
alternative and supportive agents, especially for the use of herbal products.
The purpose of this study is to evaluate the in vitro anti-tumor activity of Lysimachia savranii in breast cancer
cell lines compared to normal adipose tissue derived mesenchymal stem cells. The
adipose tissue derived mesenchymal stem cells as normal cells with MCF-7 and,
MDA-MB-231 breast cancer cells were cultured. IC₅₀ doses of Lysimachia savranii extract for each
cells were determined via MTT assay. The immunocytochemical staining was
performed for the oxidative stress by eNOS, whereas apoptosis were analyzed by
TUNEL method. The data exhibited with H-score and apoptotic index. The plant
extract inhibited the proliferation and survival in the breast cancer cells compared
to the stem cells. Lysimachia savranii
extract caused the increase of eNOS staining for oxidative stress and the TUNEL
labeling for apoptosis. The further molecular
techniques should be used to determine the biologic activity of Lysimachia
savranii extract and its content
should be investigated.

Keywords

Lysimachia savranii, breast cancer, proliferation, cytotoxicity

References

• Dai, L.M., Huang, R.Z., Zhang, B., Hua, J., Wang, H.S., Liang, D., 2017. Cytotoxic triterpenoid saponins from Lysimachia foenum-graecum. Phytochemistry 136, 165-174. doi: 10.1016/j.phytochem.2017.01.021.
• Deliloglu-Gurhan, I., Tuglu, I., Vatansever, H.S., Ozdal-Kurt, F., Ekren, H., Taylan, M., Sen, B.H., 2006. The effect of osteogenic medium on the adhesion of rat bone marrow stromal cell to the hydroxyapatite. Saudi Med J 27(3), 305-11.
• DeSantis, C.E., Fedewa, S.A., Goding Sauer, A., Kramer, J.L., Smith, R.A., Jemal, A., 2016. Breast cancer statistics, 2015: Convergence of incidence rates between black and white women. CA Cancer J Clin. 66(1), 31-42. doi: 10.3322/caac.21320.
• Fei, Z.H., Wu, K., Chen, Y.L., Wang, B., Zhang, S.R., Ma, S.L., 2014. Capilliposide Isolated from Lysimachia capillipes Hemsl. Induces ROS Generation, Cell Cycle Arrest, and Apoptosis in Human Nonsmall Cell Lung Cancer Cell Lines. Evid Based Complement Alternat Med, 497456. doi: 10.1155/2014/497456.
• Hack, C.C., Voiß, P., Lange, S., Paul, A.E., Conrad, S., Dobos, G.J., Beckmann, M.W., Kümmel, S., 2015. Local and Systemic Therapies for Breast Cancer Patients: Reducing Short-term Symptoms with the Methods of Integrative Medicine. Geburtshilfe Frauenheilkd 75(7), 675-682.
• He, Z., Liang, F., Lu, J., Pan, Y., 2013. Cytotoxic triterpenoids from Lysimachia parvifolia. Eur J Med Chem 67, 390-7. doi: 10.1016/j.ejmech.2013.06.043.
• Kim, W., Lee, W.B., Lee, J.W., Min, B.I., Baek, S.K., Lee, H.S., Cho, S.H., 2015. Traditional herbal medicine as adjunctive therapy for breast cancer: A systematic review. Complement Ther Med 23(4), 626-32. doi: 10.1016/j.ctim.2015.03.011.
• Mete, M., Aydemir, I., Ünsal, Ü.Ü., Duransoy, Y.K., Tuğlu, M.İ., Selçuki, M., 2016. Neuroprotective effects of bone marrow-derived mesenchymal stem cells and conditioned medium in mechanically injured neuroblastoma cells. Turk J Med Sci 46, 1900-1907, Özdal-Kurt, F., Tuğlu, I., Vatansever, H.S., Tong, S., Şen, B.H., Deliloğlu-Gürhan, S.I., 2016. The effect of different implant biomaterials on the behavior of canine bone marrow stromal cells during their differentiation into osteoblasts. Biotech Histochem 91(6), 412-22. doi: 10.1080/10520295.2016.1183819.
• Ozbilgin, K., Karaca, F., Turan, A., Köse, C., Vatansever, S., Ozcakir, T., 2015. The higher heparin-binding epidermal growth factor (HB-EGF) in missed abortion. Taiwan J Obstet Gynecol 54(1), 13-8. doi: 10.1016/j.tjog.2013.08.011.
• Li, R., Zhang, L., Zhang, L., Chen, D., Tian, J., Cao, L., Zhang, L., 2014. Capilliposide C derived from Lysimachia capillipes Hemsl inhibits growth of human prostate cancer PC3 cells by targeting caspase and MAPK pathways. Int Urol Nephrol 46(7), 1335-44. doi: 10.1007/s11255-013-0641-6.
• Liang, D., Hao, Z.Y., Liu, Y.F., Luo, H., Wang, Y., Zhang, C.L., Zhang, Q.J., Chen, R.Y., Yu, D.Q., 2013. Bioactive carboxylic acids from Lysimachia clethroides. J Asian Nat Prod Res 15(1), 59-66. doi: 10.1080/10286020.2012.745855.
• Lv, Y., Yang, X.B., Zhao, Y., Ruan, Y., Yang, Y., Wang, Z.Z., 2009. Separation and quantification of component monosaccharides of the tea polysaccharides from Gynostemma pentaphyllum by HPLC with indirect UV detection. Food Chemistry 112 (3), 742-746.
• Podolak, I., Koczurkiewicz, P., Michalik, M., Galanty, A., Zajdel, P., Janeczko, Z., 2013. A new cytotoxic triterpene saponin from Lysimachia nummularia L. Carbohydr Res 375, 16-20. doi: 10.1016/j.carres.2013.04.005.
• Podolak, I., Galanty, A., Janeczko, Z., 2005. Cytotoxic activity of embelin from Lysimachia punctata. Fitoterapia 76(3-4), 333-5.
• Pourheydar, B., Soleimani Asl, S., Azimzadeh, M., Rezaei Moghadam, A., Marzban, A., Mehdizadeh, M., 2016. Neuroprotective Effects of Bone Marrow Mesenchymal Stem Cells on Bilateral Common Carotid Arteries Occlusion Model of Cerebral Ischemia in Rat. Behav Neurol 2016, 2964712.Shen, Z., Xu, L., Li, J., Zhang, N., 2017. Capilliposide C Sensitizes Esophageal Squamous Carcinoma Cells to Oxaliplatin by Inducing Apoptosis Through the PI3K/Akt/mTOR Pathway. Med Sci Monit 23, 2096-2103.
• Sumalatha, K., Gowda, M., 2017. Meenakshisundaram S. ROS-mediated induction of apoptosis by benzoquinone embelin in human colon adenocarcinoma cells HT-29. J Complement Integr Med 14(2), pii:/j/jcim.2017.14.issue-2/jcim-2016-0131/jcim-2016-0131.xml. doi:10.1515/jcim-2016-0131.
• Tariq, A., Sadia, S., Pan, K., Ullah, I., Mussarat, S., Sun, F., Abiodun, O.O., Batbaatar, A., Li, Z., Song, D., Xiong, Q., Ullah, R., Khan, S., Basnet, B.B., Kumar, B., Islam, R., Adnan, M., 2017. A systematic review on ethnomedicines of anti-cancer plants. Phytother Res 31(2), 202-264. doi: 10.1002/ptr.5751.
• Torre, L.A., Siegel, R.L., Ward, E.M., Jemal, A., 2016. Global Cancer Incidence and Mortality Rates and Trends--An Update. Cancer Epidemiol Biomarkers Prev 25(1), 16-27. doi: 10.1158/1055-9965.
• Tuğlu, İ., Özdal-Kurt, F., Koca, H., Sarac, A., Barut, T., Kazanç, A., 2010. The contribution of differentiated bone marrow stromal stem cellloaded biomaterial to treatment in critical size defect model in rats. Kafkas Univ Vet Fak Derg 16 (5), 783-792. DOI:10.9775/kvfd.2010.1582.
• Trachootham, D., Alexandre, J., Huang, P., 2009. Targeting cancer cells by ROS-mediated mechanisms: a radical therapeutic approach? Nat Rev Drug Discov 8(7), 579-91. doi: 10.1038/nrd2803.
• Zhang, S.L., Yang, Z.N., He, C., Liao, H.B., Wang, H.S., Chen, Z.F., Liang, D., 2018. Oleanane-type triterpenoid saponins from Lysimachia fortunei Maxim. Phytochemistry 147, 140-146. doi: 10.1016/j.phytochem.2017.12.022.
• Zheng, J., Zhou, Y., Li, Y., Xu, D.P., Li, S., Li, H.B., 2016. Spices for Prevention and Treatment of Cancers. Nutrients 8(8), pii: E495. doi: 10.3390/nu8080495.