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Burdur İlindeki Bazı Honamlı Irkı Keçi Sürülerinde Aflatoksin M1 Düzeylerinin Belirlenmesi

Year 2022, Volume: 10 Issue: 3, 146 - 150, 31.12.2022
https://doi.org/10.24998/maeusabed.1200417

Abstract

Bu çalışmada 2021 yılında bahar mevsiminde ve Nisan ayında Burdur İlinde Honamlı ırkı keçi yetiştiriciliği yapan 12 sürüde bulunan ve yaşları 2-6 yaş arasında değişen 90 adet laktasyondaki Honamlı ırkı keçilerin süt serumlarından aflatoksin M1 (AFM1) seviyeleri belirlenmiştir. Bulgularda sürü 1 ile sürü 4,5,6,7,8,9,10,11,12 arasında ve sürü 3 ile sürü 9 ve 11 arasında istatistiksel olarak anlamlı farklılık (p<0.05) bulunmuştur. Ayrıca, Burdur iline bağlı Honamlı ırkı keçi yetiştiriciliği yapılan 12 işletmede ortalama AFM1 konsantrasyonu 29.79 ng/L olarak belirlenmiştir. 12 sürüde minimum AFM1 konsantrasyonu 7.99 ng/L maksimum AFM1 konsantrasyonu 46.28 ng/L olarak belirlenmiştir. Sonuç olarak bu çalışmada burdur ilinde çalışmaya dahil edilen 12 sürünün tamamının süt numunelerinde AFM1 tespit edilmiştir. AFM1 düzeylerinin Türk Gıda Kodeksi’ne göre kabul edilebilir düzeyde belirlenmiştir. Ancak Türk Gıda Kodeksi’ne göre kabul edilebilir olsa da sürünün tamamında AFM1 bulunması depolama şartları kötü olan yem bitkilerinin hayvanlara yedirilmesi sırasında çok dikkatli olunmalıdır. Ayrıca meralardaki bitkilerdeki aflatoksin yoğunluğu araştırılmalıdır. Hayvan beslemede son dönemlerde aktif bir şekilde kullanılan doğal toksin bağlayıcıların keçi yetiştiriciliğinde de mutlaka kullanılması gerektiği kanısına varılmıştır.

Project Number

-

References

  • Akdemir, Ç., Altıntaş, A., 2004. Ankara’da işlenen sütlerde aflatoksin-M1 varlığının ve düzeylerinin HPLC ile araştırılması. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 51(3), 174-179.
  • Battacone, G., Nudda, A., Palomba, M., Pascale, M., Nicolussi, P., Pulina, G., 2005. Transfer of aflatoxin B1 from feed to milk and from milk to curd and whey in dairy sheep fed artificially contaminated concentrates. Journal of Dairy Science, 88(9), 3063-3069.
  • Baygeldi, Y., Tanyıldızı, S., 2018. Elazığ’da Üretilen İnek Sütlerinde Aflatoksin M1 Düzeylerinin HPLC ile Araştırılması. Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi, 32(3), 191-195.
  • Bbosa, G.S., Kitya, D., & Ogwal-Okeng, J., 2013. Aflatoxins metabolism, effects on epigenetic mechanisms and their role in carcinogenesis. 5, 14-34.
  • Betina, V., 1989. Aflatoxins, sterigmatocystins and versicolorins. Mycotoxins: Chemical, Biological and Environmental Aspects. Elsevier, Amsterdam-Oxford-New York-Tokyo.
  • Bilandžić, N., Božić, Đ., Đokić, M., Sedak, M., Kolanović, B S., Varenina, I., Cvetnić, Ž., 2014. Assessment of aflatoxin M1 contamination in the milk of four dairy species in Croatia. Food control, 43, 18-21.
  • Çankırı, B., Uyarlar, C., 2013. Mikotoksinlerin süt sığırlarının beslenmesindeki yeri ve önemi. Kocatepe Veterinary Journal, 6(2), 57-69.
  • Ergün, S.G., Özkan, S., & Abbasoğlu, U., 2016. Investigation of aflatoxin B1 levels in red-scaled pepper by ELISA. Gazi Medical Journal, 27(4).
  • Frobish, R.A., Bradley, B.D., Wagner, D.D., Long-Bradley, P E., Hairston, H., 1986. Aflatoxin residues in milk of dairy cows after ingestion of naturally contaminated grain. Journal of food protection, 49(10), 781-785.
  • Ghanem, I., Orfi, M,. 2009. Aflatoxin M1 in raw, pasteurized and powdered milk available in the Syrian market. Food Control, 20(6), 603-605.
  • Goto, T., Hsieh, D.P., 1985. Fractionation of radioactivity in the milk of goats administered 14C-Aflatoxin B1. Journal of the Association of Official Analytical Chemists, 68(3), 456-458.
  • Hussain, I., Anwar, J., Asi, M.R., Munawar, M.A., Kashif, M., 2010. Aflatoxin M1 contamination in milk from five dairy species in Pakistan. Food control, 21(2), 122-124.
  • İşleyici, Ö., Sancak, Y.C., Sancak, H., Yücel, U.M., 2015. Determination of Aflatoxin M1 Levels in Unpackaged Sold Raw Cow's Milk. Van Veterinary Journal, 26(3), 151-155.
  • Karadal, F., ONMAZ, N. E., HIZLISOY, H., YILDIRIM, Y., Serhat, A. L., Gönülalan, Z., 2018. Niğde ilindeki çiğ koyun, keçi ve inek sütlerinde aflatoksin M1 düzeyleri. Kocatepe Veterinary Journal, 11(2), 119-125.
  • Kaya, S., Pirinçci, İ., Bilgili, A., 2002. Veteriner hekimliğinde toksikoloji. Medisan Yayınevi.
  • Kaya Tuz, M., Asan, A., Ökten, S., 2017. Devam sütlerinde Aflatoksin M1 varlığının elisa yöntemiyle tespit Edilmesi. Trakya University Journal of Natural Sciences, 18(1), 55-58.
  • Martins, M.L., & Martins, H.M., 2000. Aflatoxin M1 in raw and ultra high temperature-treated milk commercialized in Portugal. Food additives & contaminants, 17(10), 871-874.
  • Molle, G., Decandia, M., Cabiddu, A., Landau, S.Y., Cannas, A., 2008. An update on the nutrition of dairy sheep grazing Mediterranean pastures. Small Ruminant Research, 77(2-3), 93-112.
  • Navarro, S., & Zettler, J.L., 2001. Critical limits of sealing for successful application of controlled atmosphere or fumigation. In Proc. Int. Conf. on Controlled Atmosphere and Fumigation in Stored Products. Executive Printing Services, Clovis, CA, 507-520.
  • Pittet, A. 1998. Natural occurrence of mycotoxins in foods and feeds: An update review. Revue de Medecine Veterinaire, 149, 479-492.
  • Özdemir, M., 2007. Determination of aflatoxin M1 levels in goat milk consumed in Kilis province. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 54(2), 99-103.
  • Özkaya, Ş., Temiz, A., 2003. Aflatoksinler: Kimyasal yapıları, toksisiteleri ve detoksifikasyonları. Orlab On-Line Mikrobiyoloji Dergisi, 1(1), 1-21.
  • Pohland, A.E., 1993. Mycotoxins in Review. Food Addivites and Contaminant,s 10(1), 17-28.
  • Rahimi, E., Ameri, M., 2012. A survey of aflatoxin M1 contamination in bulk milk samples from dairy bovine, ovine, and caprine herds in Iran. Bulletin of Environmental Contamination and Toxicology, 89(1), 158-160.
  • Rao, S.N., Chopra, R.C., 2001. Influence of sodium bentonite and activated charcoal on aflatoxin M1 excretion in milk of goats. Small Ruminant Research, 41(3), 203-213.
  • Ronchi, B., Danieli, P. P., Vitali, A., Sabatini, A., Bernabucci, U., Nardone, A., 2005. Evaluation of AFB1/AFM1 carry-over in lactating goats exposed to different levels of AFB1 contamination. Proc 56th Ann Meet EAAP, Uppsala, Sweden, 1-10.
  • Türk Gıda Kodeksi Tebliğ., 2002. Türk Gıda Kodeksi gıda maddelerinde belirli bulaşanların maksimum seviyelerinin belirlenmesi hakkında tebliğ (No:2002/63). Resmi Gazete, 23.09.2002, Sayı: 24885.
  • Türel, G.Y., Calapoğlu, N.Ş., 2017. Mikotoksinler ve moleküler düzeydeki etkileri. SDÜ Tıp Fakültesi Dergisi, 24(1), 13-28.
  • Van Egmond, H.P., 1989. Aflatoxin M1: occurrence, toxicity, regulation. In H.P. Van Egmond (Ed), Mycotoxins in Dairy Products. Elsevier Applied Science, New York. 11-55.
  • Veldman, A., Meijs, J.A.C., Borggreve, G.J., & Heeres-Van Der Tol, J J., 1992. Carry-over of aflatoxin from cows' food to milk. Animal Science, 55(2), 163-168.
  • Virdis, S., Scarano, C., Spanu, V., Murittu, G., Spanu, C., Ibba, I., & De Santis, E.P.L., 2014. A survey on Aflatoxin M1 content in sheep and goat milk produced in Sardinia Region, Italy (2005-2013). Italian journal of food safety, 3(4), 4517.
  • Van Egmond, H.P., 1989. Current situation on regulations for mycotoxins. Overview of tolerances and status of standard methods of sampling and analysis. Food Additives & Contaminants, 6(2), 139-188.
  • Whitlow, L.W., Hagler, W.M., Diaz, D.E., 2010. Mycotoxins in feeds. Foodstuffs , 74-84. Aksoy, U., 1990. Aflatoksin. Ege Üniversitesi Tarımsal Uygulama ve Araştırmalar Merkezi, 2, 1-7.

Determination of Aflatoxin M1 Levels in Some Honamli Goat Herds in Burdur Province

Year 2022, Volume: 10 Issue: 3, 146 - 150, 31.12.2022
https://doi.org/10.24998/maeusabed.1200417

Abstract

In this study, aflatoxin M1 (AFM1) levels were determined from the milk serum of 90 lactating Honamlı goats aged 2-6 years in 12 herds breeding Honamlı goats in Burdur Province in spring and April in 2021. A statistically significant difference was found between flock 1 and flock 4,5,6,7,8,9,10,11,12 and between flock 3 and flock 9 and 11 (p<0.05). In addition, the mean AFM1 concentration was determined as 29.79 ng/L in 12 farms of Honamlı breed goat breeding in Burdur province. The minimum AFM1 concentration was 7.99 ng/L and the maximum AFM1 concentration was 46.28 ng/L in 12 flocks. As a result, AFM1 was detected in the milk samples of all 12 herds included in the Burdur study in this study. AFM1 levels were determined at an acceptable level according to the Turkish Food Codex. However, although it is acceptable according to the Turkish Food Codex, the presence of AFM1 in the whole herd should be very careful when feeding the fodder plants with poor storage conditions to the animals. In addition, the concentration of aflatoxin in plants in pastures should be investigated. This study has proven that natural toxin binders, which have been actively used in animal nutrition recently, should also be used in goat breeding.

Supporting Institution

-

Project Number

-

Thanks

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References

  • Akdemir, Ç., Altıntaş, A., 2004. Ankara’da işlenen sütlerde aflatoksin-M1 varlığının ve düzeylerinin HPLC ile araştırılması. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 51(3), 174-179.
  • Battacone, G., Nudda, A., Palomba, M., Pascale, M., Nicolussi, P., Pulina, G., 2005. Transfer of aflatoxin B1 from feed to milk and from milk to curd and whey in dairy sheep fed artificially contaminated concentrates. Journal of Dairy Science, 88(9), 3063-3069.
  • Baygeldi, Y., Tanyıldızı, S., 2018. Elazığ’da Üretilen İnek Sütlerinde Aflatoksin M1 Düzeylerinin HPLC ile Araştırılması. Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi, 32(3), 191-195.
  • Bbosa, G.S., Kitya, D., & Ogwal-Okeng, J., 2013. Aflatoxins metabolism, effects on epigenetic mechanisms and their role in carcinogenesis. 5, 14-34.
  • Betina, V., 1989. Aflatoxins, sterigmatocystins and versicolorins. Mycotoxins: Chemical, Biological and Environmental Aspects. Elsevier, Amsterdam-Oxford-New York-Tokyo.
  • Bilandžić, N., Božić, Đ., Đokić, M., Sedak, M., Kolanović, B S., Varenina, I., Cvetnić, Ž., 2014. Assessment of aflatoxin M1 contamination in the milk of four dairy species in Croatia. Food control, 43, 18-21.
  • Çankırı, B., Uyarlar, C., 2013. Mikotoksinlerin süt sığırlarının beslenmesindeki yeri ve önemi. Kocatepe Veterinary Journal, 6(2), 57-69.
  • Ergün, S.G., Özkan, S., & Abbasoğlu, U., 2016. Investigation of aflatoxin B1 levels in red-scaled pepper by ELISA. Gazi Medical Journal, 27(4).
  • Frobish, R.A., Bradley, B.D., Wagner, D.D., Long-Bradley, P E., Hairston, H., 1986. Aflatoxin residues in milk of dairy cows after ingestion of naturally contaminated grain. Journal of food protection, 49(10), 781-785.
  • Ghanem, I., Orfi, M,. 2009. Aflatoxin M1 in raw, pasteurized and powdered milk available in the Syrian market. Food Control, 20(6), 603-605.
  • Goto, T., Hsieh, D.P., 1985. Fractionation of radioactivity in the milk of goats administered 14C-Aflatoxin B1. Journal of the Association of Official Analytical Chemists, 68(3), 456-458.
  • Hussain, I., Anwar, J., Asi, M.R., Munawar, M.A., Kashif, M., 2010. Aflatoxin M1 contamination in milk from five dairy species in Pakistan. Food control, 21(2), 122-124.
  • İşleyici, Ö., Sancak, Y.C., Sancak, H., Yücel, U.M., 2015. Determination of Aflatoxin M1 Levels in Unpackaged Sold Raw Cow's Milk. Van Veterinary Journal, 26(3), 151-155.
  • Karadal, F., ONMAZ, N. E., HIZLISOY, H., YILDIRIM, Y., Serhat, A. L., Gönülalan, Z., 2018. Niğde ilindeki çiğ koyun, keçi ve inek sütlerinde aflatoksin M1 düzeyleri. Kocatepe Veterinary Journal, 11(2), 119-125.
  • Kaya, S., Pirinçci, İ., Bilgili, A., 2002. Veteriner hekimliğinde toksikoloji. Medisan Yayınevi.
  • Kaya Tuz, M., Asan, A., Ökten, S., 2017. Devam sütlerinde Aflatoksin M1 varlığının elisa yöntemiyle tespit Edilmesi. Trakya University Journal of Natural Sciences, 18(1), 55-58.
  • Martins, M.L., & Martins, H.M., 2000. Aflatoxin M1 in raw and ultra high temperature-treated milk commercialized in Portugal. Food additives & contaminants, 17(10), 871-874.
  • Molle, G., Decandia, M., Cabiddu, A., Landau, S.Y., Cannas, A., 2008. An update on the nutrition of dairy sheep grazing Mediterranean pastures. Small Ruminant Research, 77(2-3), 93-112.
  • Navarro, S., & Zettler, J.L., 2001. Critical limits of sealing for successful application of controlled atmosphere or fumigation. In Proc. Int. Conf. on Controlled Atmosphere and Fumigation in Stored Products. Executive Printing Services, Clovis, CA, 507-520.
  • Pittet, A. 1998. Natural occurrence of mycotoxins in foods and feeds: An update review. Revue de Medecine Veterinaire, 149, 479-492.
  • Özdemir, M., 2007. Determination of aflatoxin M1 levels in goat milk consumed in Kilis province. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 54(2), 99-103.
  • Özkaya, Ş., Temiz, A., 2003. Aflatoksinler: Kimyasal yapıları, toksisiteleri ve detoksifikasyonları. Orlab On-Line Mikrobiyoloji Dergisi, 1(1), 1-21.
  • Pohland, A.E., 1993. Mycotoxins in Review. Food Addivites and Contaminant,s 10(1), 17-28.
  • Rahimi, E., Ameri, M., 2012. A survey of aflatoxin M1 contamination in bulk milk samples from dairy bovine, ovine, and caprine herds in Iran. Bulletin of Environmental Contamination and Toxicology, 89(1), 158-160.
  • Rao, S.N., Chopra, R.C., 2001. Influence of sodium bentonite and activated charcoal on aflatoxin M1 excretion in milk of goats. Small Ruminant Research, 41(3), 203-213.
  • Ronchi, B., Danieli, P. P., Vitali, A., Sabatini, A., Bernabucci, U., Nardone, A., 2005. Evaluation of AFB1/AFM1 carry-over in lactating goats exposed to different levels of AFB1 contamination. Proc 56th Ann Meet EAAP, Uppsala, Sweden, 1-10.
  • Türk Gıda Kodeksi Tebliğ., 2002. Türk Gıda Kodeksi gıda maddelerinde belirli bulaşanların maksimum seviyelerinin belirlenmesi hakkında tebliğ (No:2002/63). Resmi Gazete, 23.09.2002, Sayı: 24885.
  • Türel, G.Y., Calapoğlu, N.Ş., 2017. Mikotoksinler ve moleküler düzeydeki etkileri. SDÜ Tıp Fakültesi Dergisi, 24(1), 13-28.
  • Van Egmond, H.P., 1989. Aflatoxin M1: occurrence, toxicity, regulation. In H.P. Van Egmond (Ed), Mycotoxins in Dairy Products. Elsevier Applied Science, New York. 11-55.
  • Veldman, A., Meijs, J.A.C., Borggreve, G.J., & Heeres-Van Der Tol, J J., 1992. Carry-over of aflatoxin from cows' food to milk. Animal Science, 55(2), 163-168.
  • Virdis, S., Scarano, C., Spanu, V., Murittu, G., Spanu, C., Ibba, I., & De Santis, E.P.L., 2014. A survey on Aflatoxin M1 content in sheep and goat milk produced in Sardinia Region, Italy (2005-2013). Italian journal of food safety, 3(4), 4517.
  • Van Egmond, H.P., 1989. Current situation on regulations for mycotoxins. Overview of tolerances and status of standard methods of sampling and analysis. Food Additives & Contaminants, 6(2), 139-188.
  • Whitlow, L.W., Hagler, W.M., Diaz, D.E., 2010. Mycotoxins in feeds. Foodstuffs , 74-84. Aksoy, U., 1990. Aflatoksin. Ege Üniversitesi Tarımsal Uygulama ve Araştırmalar Merkezi, 2, 1-7.
There are 33 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Research Article
Authors

Hale Ergin Eğritağ 0000-0003-4240-4698

Kemal Varol 0000-0002-3057-2865

Project Number -
Publication Date December 31, 2022
Submission Date November 7, 2022
Published in Issue Year 2022 Volume: 10 Issue: 3

Cite

APA Ergin Eğritağ, H., & Varol, K. (2022). Determination of Aflatoxin M1 Levels in Some Honamli Goat Herds in Burdur Province. Mehmet Akif Ersoy University Journal of Health Sciences Institute, 10(3), 146-150. https://doi.org/10.24998/maeusabed.1200417
AMA Ergin Eğritağ H, Varol K. Determination of Aflatoxin M1 Levels in Some Honamli Goat Herds in Burdur Province. Mehmet Akif Ersoy University Journal of Health Sciences Institute. December 2022;10(3):146-150. doi:10.24998/maeusabed.1200417
Chicago Ergin Eğritağ, Hale, and Kemal Varol. “Determination of Aflatoxin M1 Levels in Some Honamli Goat Herds in Burdur Province”. Mehmet Akif Ersoy University Journal of Health Sciences Institute 10, no. 3 (December 2022): 146-50. https://doi.org/10.24998/maeusabed.1200417.
EndNote Ergin Eğritağ H, Varol K (December 1, 2022) Determination of Aflatoxin M1 Levels in Some Honamli Goat Herds in Burdur Province. Mehmet Akif Ersoy University Journal of Health Sciences Institute 10 3 146–150.
IEEE H. Ergin Eğritağ and K. Varol, “Determination of Aflatoxin M1 Levels in Some Honamli Goat Herds in Burdur Province”, Mehmet Akif Ersoy University Journal of Health Sciences Institute, vol. 10, no. 3, pp. 146–150, 2022, doi: 10.24998/maeusabed.1200417.
ISNAD Ergin Eğritağ, Hale - Varol, Kemal. “Determination of Aflatoxin M1 Levels in Some Honamli Goat Herds in Burdur Province”. Mehmet Akif Ersoy University Journal of Health Sciences Institute 10/3 (December 2022), 146-150. https://doi.org/10.24998/maeusabed.1200417.
JAMA Ergin Eğritağ H, Varol K. Determination of Aflatoxin M1 Levels in Some Honamli Goat Herds in Burdur Province. Mehmet Akif Ersoy University Journal of Health Sciences Institute. 2022;10:146–150.
MLA Ergin Eğritağ, Hale and Kemal Varol. “Determination of Aflatoxin M1 Levels in Some Honamli Goat Herds in Burdur Province”. Mehmet Akif Ersoy University Journal of Health Sciences Institute, vol. 10, no. 3, 2022, pp. 146-50, doi:10.24998/maeusabed.1200417.
Vancouver Ergin Eğritağ H, Varol K. Determination of Aflatoxin M1 Levels in Some Honamli Goat Herds in Burdur Province. Mehmet Akif Ersoy University Journal of Health Sciences Institute. 2022;10(3):146-50.