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Diagnosis of Mycoplasma agalactiae from various specimens of goats

Year 2024, Volume: 12 Issue: 1, 40 - 45, 02.05.2024
https://doi.org/10.24998/maeusabed.1329900

Abstract

The aim of this study was to determine the presence of Mycoplasma agalactiae (M. agalactiae) by bacteriological methods and to reveal the prevalence of the disease in a goat farm with pneumonia, mastitis and arthritis symptoms and deaths. This study was carried out in a Saanen goat farm in Isparta province between January 2015 and January 2017. Samples (milk, intra-articular fluid, internal organs of deceased animals) were brought to Burdur Mehmet Akif Ersoy University, Faculty of Veterinary Medicine Microbiology Laboratory and examined by bacteriological methods. Blood samples were collected from 813 goats in aged 1-6 years in the goat farm. Blood sera were tested for Mycoplasma agalactiae with a commercial ELISA kit (IDEXX, France) according to the kit instructions. Mycoplasma colonies were isolated from all samples. As a result of serological examination, 83 (10.2%) animals were positive, 9 (1.1%) animals were suspicious, and the rest were negative by ELISA. The seropositivity of M. agalactiae was ranged from 5.1 % to 28.7% according to age, and it was found quite high in three-year-old animals. With this study, it was determined that the seroprevalance of M. agalactiae is quite high in goats aged three years and above, it can be detected of M. agalactiae in animals that do not show clinical symptoms using ELISA and can be used in eradication of the disease from flocks.

References

  • Assunçao, P., Davey, H.M., Rosales, R.S., Antunes, N.T., De la Fe, C., Ramirez, A.S., Ruiz de Galarreta, C.M., Poveda, J.B., 2007. Detection of mycoplasmas in goat milk by flow cytomery. Cytometry Part A, 71A, 1034-1038.
  • Azevedo, E.O.D., Alcântara, M.D.B.D., Nascimento, E.R.D., Tabosa, I.M., Barreto, M.L., Almeida, J.F.D., Castro, R.S.D., 2006. Contagious agalactia by Mycoplasma agalactiae in small ruminants in Brazil: first report. Brazilian Journal of Microbiology 37, 576-581.
  • Bandeira, D.A., Castro, R.S., Azevedo, E.O., Nascimento, E.R., Melo, L.S.S., Melo, C.B., 2008. Infection by Mycoplasma agalactiae in dairy goat herds in the microregions of Cariri in Paraíba State, Brazil. Arquivo Brasileiro de Medicina Veterinaria e Zootecnia 60 (5), 1255-1258.
  • Beşe, M., Arda, M., 1968. Koyunlarda Mycoplasma agalactiae’nin ilk izolasyonu üzerinde araştırma. https://dspace.ankara.edu.tr/xmlui/bitstream/handle/20.500.12575/57446/8135.pdf? sequence=1&isAllowed=y. (Erişim 3.10.2022).
  • Bohach, D.M., Stegniy, B.T., Bohach, M.V., Pavlov, S.L., Bolotin, V.I., 2021. Age and seasonal pattern of contagious agalactia in small ruminants in Ukraine. Journal of Veterinary Research 65(1), 67-72.
  • Cottew, G.S., Watson, W.A., Arısoy, F., Erdağ, O., Buckley, L.S., 1968. Differntiation of Mycoplasma agalactiae from other mycoplasmas of sheep and goats. Journal of Comparative Pathology 78, 275-282.
  • Çetinkaya, B., Kalın, R., Karahan, M., Atıl, E., Manso-Sılvan, L., Thıaucourt, F., 2009. Detection of contagious caprine pleuropneumonia in East Turkey. Revue Scientifique et Technique İnternational Office of Epiootic 28(3), 1037-1044.
  • Çetinkaya, B., Karahan, M., Kalın, R., Atıl, E., 2010. Türkiye’nin doğusundaki ruminant Mikoplazmalarının biyoçeşitliliği: Aşı ve kontrol stratejileri için uygulamalar. IX. Ulusal Veteriner Mikrobiyoloji Kongresi Bildiri, Lefkoşa, Kuzey Kıbrıs Türk Cumhuriyeti, sayfa 9-12.
  • Gomez-Martin, A., Amores, J., Paterna, A., De la fe, C., 2013. Contagious agalactia due to Mycoplasma spp. in small dairy ruminants: Epidemiology and prospects for diagnosis and control. The Veterinary Journal 198, 48-56.
  • Göçmen, H., Ülgen, M., Çarlı, K.T., Önat, K., Kahya, S., Özdemir, Ü., Mat, B., 2015. Koyun ve keçilerde bulaşıcı agalaksi hastalığının bakteriyolojik ve PCR metotları ile araştırılması. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 21(1), 75-80.
  • Jaÿ, M., Tardy, F., 2019. Contagious agalactia in sheep and goats: current perspectives. Veterinary Medicine: Research and Reports 10, 229–247.
  • Keskin, D., 2018. Koyun ve keçilerde Mycoplasma agalactiae’nin önemi. e-TRALLEIS., 2, 1-5.
  • Kızıl, O., Ozdemir, H., 2006. Clinical, haematological and biochemical studies in goats naturally infected with Mycoplama agalactiae. Bulletion of the Veterinary Institute in Pulawy 50, 325-328.
  • Kinde, H., Damassa, A.J., Wakenell, P.S., Petty, R., 1994. Mycoplasma infection in commercial goat dairy caused by Mycoplasma agalactiae and Mycoplasma mycoides subsp. mycoides (caprine biotype). Journal of Veterinary Diagnostic Investigation 6, 423-427.
  • Kumar, A., Rahal, A., Chakraborty, S., Kumar Verma, A., Dhama, K., 2014. Mycoplasma agalactiae, an etiological agent of contagious agalactia in small ruminants: a review. Veteriner Medicine International, (http://dx.doi.org/10.1155/2014/286752).
  • Lin, H., Toung, O. P., Weng, H. X., Hsu, P., Chang, M. H., Lai, J., 2022. The prevalence and species of Mycoplasma identified from dairy goats in Taiwan. Research in Veterinary Science 151, 184-188.
  • Mohan, K., Uzoukwu, M., 1985. Mycoplasma spp. from pneumonic goats and sheep in eastern Nigeria. Bulletin of Animal Health and Production in Africa 33(3), 235-239.
  • Ongor, H., Kalin, R., & Acik, M. N. (2011). Detection of Mycoplasma ovipneumoniae from goats with nasal discharge by culture and polymerase chain reaction. Pakistan Veterinary Journal 31(3), 244-248.
  • Reji, R.M., Mini, M., Mani, B.K., Sankar, S., Thirupathy, V., Reshma, P.S., AIswarya, N., Akkara, T.S., 2018. Isolation and identification of Mycoplasma agalactiae associated with respiratory tract infection in goats: a case study. The Pharma Innovation Journal 7(12), 21-23.
  • Şababoğlu, E., Yapıcıer, Ö.Ş., Öztürk, D., Pehlivanoğlu, F., Kaya, M., Türütoğlu, 2018. Buzağı artritlerinden izole edilen bakteriyel etkenler: retrospektif bir çalışma. Veterinary Journal of Mehmet Akif Ersoy University 3(1), 36-42.
  • Verbisck-Bucker, G., González-Candela, M., Galián, J., Cubero-Pablo, M. J., Martín-Atance, P., León-Vizcaíno, L., 2008. Epidemiology of Mycoplasma agalactiae infection in free-ranging Spanish ibex (Capra pyrenaica) in Andalusia, southern Spain. Journal of Wildlife Diseases 44(2), 369-380.
  • Watson, W.A., Cottew, G.S., Erdağ, O., Arısoy, F., 1968. The pathogenicity of Mycoplasma organisms isolated from sheep and goats in Turkey. Journal of Comparative Pathology 78, 283-291.
  • World Organıiation For Animal Health (OIE) 2008. Contagious agalactia Terrestrial Manual, Chapter 2.7. 5, 992-997.
  • Zendulkova, D., Ball, H.J., Madanat, A., Lany, P., Pospisıl, Z., 2004. Detection of Mycoplasma agalactiae antigen in sheep and goats by monoclonal antibody- based sandwich ELISA. Acta Veterianaria Brno 73, 461-464.
  • Zendulkova, D., Madanat, A., Lany, P., 2007. Detection of Mycoplasma agalactiae by polymerase chain reaction in jordanian sheep and goat herds. Acta Veterianaria Brno 76, 71-77.

Keçilerin çeşitli örneklerinden Mycoplasma agalactiae’nın teşhisi

Year 2024, Volume: 12 Issue: 1, 40 - 45, 02.05.2024
https://doi.org/10.24998/maeusabed.1329900

Abstract

Bu çalışmanın amacı, pnömoni, mastitis ve artritis sempomları ile birlikte ölümler görülen bir keçi çiftliğinde Mycoplasma agalactiae’nın varlığını bakteriyolojik yöntemlerle belirlemek ve hastalığın prevalansını ortaya koymaktır. Bu çalışma 2015 Ocak- 2017 Ocak ayları arasında Isparta ilinde bir Saanen keçi çiftliğinde yapıldı. Burdur Mehmet Akif Ersoy Üniversitesi Veteriner Fakültesi Mikrobiyoloji Laboratuvarı’na getirilen örnekler (hangi örnekler) bakteriyolojik metotlarla incelendi. Ayrıca keçi çiftliğinde yaşları 1-6 arasında değişen 813 keçiden kan örnekleri toplandı. Kan serumları M. agalactiae varlığı yönünden ticari bir ELISA kiti (IDEXX, France) ile kit prospektüsüne uygun olarak test edildi. Örneklerden Pleura Pneumonia Like Organism (PPLO) besiyerine ekimler yapıldı ve Mycoplasma spp. kolonileri tüm örneklerden saf olarak izole edildi. Serolojik muayene sonucunda ELISA ile 83 (%10.2) hayvan pozitif, 9 (%1.1) hayvan şüpheli ve geri kalanı negatif olarak saptandı. M. agalactiae enfeksiyonunun seropozitifliği, yaşlara göre değerlendirildiğinde, %5.1 ile %28.7 arasında değiştiği, üç yaşındaki hayvanlarda oldukça yüksek olduğu belirlendi. Bu çalışma ile keçilerde M. agalactiae’nın seroprevalansının üç yaş ve üzerindeki keçilerde oldukça yüksek olduğu, semptom göstermeyen hayvanlarda ELISA pozitif hayvanların tespit edilebileceği, sürülerden hastalığın eradikasyonunda ELISA testinin kullanılabileceği kanaatine varıldı.

References

  • Assunçao, P., Davey, H.M., Rosales, R.S., Antunes, N.T., De la Fe, C., Ramirez, A.S., Ruiz de Galarreta, C.M., Poveda, J.B., 2007. Detection of mycoplasmas in goat milk by flow cytomery. Cytometry Part A, 71A, 1034-1038.
  • Azevedo, E.O.D., Alcântara, M.D.B.D., Nascimento, E.R.D., Tabosa, I.M., Barreto, M.L., Almeida, J.F.D., Castro, R.S.D., 2006. Contagious agalactia by Mycoplasma agalactiae in small ruminants in Brazil: first report. Brazilian Journal of Microbiology 37, 576-581.
  • Bandeira, D.A., Castro, R.S., Azevedo, E.O., Nascimento, E.R., Melo, L.S.S., Melo, C.B., 2008. Infection by Mycoplasma agalactiae in dairy goat herds in the microregions of Cariri in Paraíba State, Brazil. Arquivo Brasileiro de Medicina Veterinaria e Zootecnia 60 (5), 1255-1258.
  • Beşe, M., Arda, M., 1968. Koyunlarda Mycoplasma agalactiae’nin ilk izolasyonu üzerinde araştırma. https://dspace.ankara.edu.tr/xmlui/bitstream/handle/20.500.12575/57446/8135.pdf? sequence=1&isAllowed=y. (Erişim 3.10.2022).
  • Bohach, D.M., Stegniy, B.T., Bohach, M.V., Pavlov, S.L., Bolotin, V.I., 2021. Age and seasonal pattern of contagious agalactia in small ruminants in Ukraine. Journal of Veterinary Research 65(1), 67-72.
  • Cottew, G.S., Watson, W.A., Arısoy, F., Erdağ, O., Buckley, L.S., 1968. Differntiation of Mycoplasma agalactiae from other mycoplasmas of sheep and goats. Journal of Comparative Pathology 78, 275-282.
  • Çetinkaya, B., Kalın, R., Karahan, M., Atıl, E., Manso-Sılvan, L., Thıaucourt, F., 2009. Detection of contagious caprine pleuropneumonia in East Turkey. Revue Scientifique et Technique İnternational Office of Epiootic 28(3), 1037-1044.
  • Çetinkaya, B., Karahan, M., Kalın, R., Atıl, E., 2010. Türkiye’nin doğusundaki ruminant Mikoplazmalarının biyoçeşitliliği: Aşı ve kontrol stratejileri için uygulamalar. IX. Ulusal Veteriner Mikrobiyoloji Kongresi Bildiri, Lefkoşa, Kuzey Kıbrıs Türk Cumhuriyeti, sayfa 9-12.
  • Gomez-Martin, A., Amores, J., Paterna, A., De la fe, C., 2013. Contagious agalactia due to Mycoplasma spp. in small dairy ruminants: Epidemiology and prospects for diagnosis and control. The Veterinary Journal 198, 48-56.
  • Göçmen, H., Ülgen, M., Çarlı, K.T., Önat, K., Kahya, S., Özdemir, Ü., Mat, B., 2015. Koyun ve keçilerde bulaşıcı agalaksi hastalığının bakteriyolojik ve PCR metotları ile araştırılması. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 21(1), 75-80.
  • Jaÿ, M., Tardy, F., 2019. Contagious agalactia in sheep and goats: current perspectives. Veterinary Medicine: Research and Reports 10, 229–247.
  • Keskin, D., 2018. Koyun ve keçilerde Mycoplasma agalactiae’nin önemi. e-TRALLEIS., 2, 1-5.
  • Kızıl, O., Ozdemir, H., 2006. Clinical, haematological and biochemical studies in goats naturally infected with Mycoplama agalactiae. Bulletion of the Veterinary Institute in Pulawy 50, 325-328.
  • Kinde, H., Damassa, A.J., Wakenell, P.S., Petty, R., 1994. Mycoplasma infection in commercial goat dairy caused by Mycoplasma agalactiae and Mycoplasma mycoides subsp. mycoides (caprine biotype). Journal of Veterinary Diagnostic Investigation 6, 423-427.
  • Kumar, A., Rahal, A., Chakraborty, S., Kumar Verma, A., Dhama, K., 2014. Mycoplasma agalactiae, an etiological agent of contagious agalactia in small ruminants: a review. Veteriner Medicine International, (http://dx.doi.org/10.1155/2014/286752).
  • Lin, H., Toung, O. P., Weng, H. X., Hsu, P., Chang, M. H., Lai, J., 2022. The prevalence and species of Mycoplasma identified from dairy goats in Taiwan. Research in Veterinary Science 151, 184-188.
  • Mohan, K., Uzoukwu, M., 1985. Mycoplasma spp. from pneumonic goats and sheep in eastern Nigeria. Bulletin of Animal Health and Production in Africa 33(3), 235-239.
  • Ongor, H., Kalin, R., & Acik, M. N. (2011). Detection of Mycoplasma ovipneumoniae from goats with nasal discharge by culture and polymerase chain reaction. Pakistan Veterinary Journal 31(3), 244-248.
  • Reji, R.M., Mini, M., Mani, B.K., Sankar, S., Thirupathy, V., Reshma, P.S., AIswarya, N., Akkara, T.S., 2018. Isolation and identification of Mycoplasma agalactiae associated with respiratory tract infection in goats: a case study. The Pharma Innovation Journal 7(12), 21-23.
  • Şababoğlu, E., Yapıcıer, Ö.Ş., Öztürk, D., Pehlivanoğlu, F., Kaya, M., Türütoğlu, 2018. Buzağı artritlerinden izole edilen bakteriyel etkenler: retrospektif bir çalışma. Veterinary Journal of Mehmet Akif Ersoy University 3(1), 36-42.
  • Verbisck-Bucker, G., González-Candela, M., Galián, J., Cubero-Pablo, M. J., Martín-Atance, P., León-Vizcaíno, L., 2008. Epidemiology of Mycoplasma agalactiae infection in free-ranging Spanish ibex (Capra pyrenaica) in Andalusia, southern Spain. Journal of Wildlife Diseases 44(2), 369-380.
  • Watson, W.A., Cottew, G.S., Erdağ, O., Arısoy, F., 1968. The pathogenicity of Mycoplasma organisms isolated from sheep and goats in Turkey. Journal of Comparative Pathology 78, 283-291.
  • World Organıiation For Animal Health (OIE) 2008. Contagious agalactia Terrestrial Manual, Chapter 2.7. 5, 992-997.
  • Zendulkova, D., Ball, H.J., Madanat, A., Lany, P., Pospisıl, Z., 2004. Detection of Mycoplasma agalactiae antigen in sheep and goats by monoclonal antibody- based sandwich ELISA. Acta Veterianaria Brno 73, 461-464.
  • Zendulkova, D., Madanat, A., Lany, P., 2007. Detection of Mycoplasma agalactiae by polymerase chain reaction in jordanian sheep and goat herds. Acta Veterianaria Brno 76, 71-77.
There are 25 citations in total.

Details

Primary Language English
Subjects Veterinary Microbiology
Journal Section Case Report
Authors

Dilek Öztürk 0000-0002-9643-8570

Sibel Yaman 0000-0002-9998-3806

Publication Date May 2, 2024
Submission Date July 19, 2023
Published in Issue Year 2024 Volume: 12 Issue: 1

Cite

APA Öztürk, D., & Yaman, S. (2024). Diagnosis of Mycoplasma agalactiae from various specimens of goats. Mehmet Akif Ersoy University Journal of Health Sciences Institute, 12(1), 40-45. https://doi.org/10.24998/maeusabed.1329900
AMA Öztürk D, Yaman S. Diagnosis of Mycoplasma agalactiae from various specimens of goats. Mehmet Akif Ersoy University Journal of Health Sciences Institute. May 2024;12(1):40-45. doi:10.24998/maeusabed.1329900
Chicago Öztürk, Dilek, and Sibel Yaman. “Diagnosis of Mycoplasma Agalactiae from Various Specimens of Goats”. Mehmet Akif Ersoy University Journal of Health Sciences Institute 12, no. 1 (May 2024): 40-45. https://doi.org/10.24998/maeusabed.1329900.
EndNote Öztürk D, Yaman S (May 1, 2024) Diagnosis of Mycoplasma agalactiae from various specimens of goats. Mehmet Akif Ersoy University Journal of Health Sciences Institute 12 1 40–45.
IEEE D. Öztürk and S. Yaman, “Diagnosis of Mycoplasma agalactiae from various specimens of goats”, Mehmet Akif Ersoy University Journal of Health Sciences Institute, vol. 12, no. 1, pp. 40–45, 2024, doi: 10.24998/maeusabed.1329900.
ISNAD Öztürk, Dilek - Yaman, Sibel. “Diagnosis of Mycoplasma Agalactiae from Various Specimens of Goats”. Mehmet Akif Ersoy University Journal of Health Sciences Institute 12/1 (May 2024), 40-45. https://doi.org/10.24998/maeusabed.1329900.
JAMA Öztürk D, Yaman S. Diagnosis of Mycoplasma agalactiae from various specimens of goats. Mehmet Akif Ersoy University Journal of Health Sciences Institute. 2024;12:40–45.
MLA Öztürk, Dilek and Sibel Yaman. “Diagnosis of Mycoplasma Agalactiae from Various Specimens of Goats”. Mehmet Akif Ersoy University Journal of Health Sciences Institute, vol. 12, no. 1, 2024, pp. 40-45, doi:10.24998/maeusabed.1329900.
Vancouver Öztürk D, Yaman S. Diagnosis of Mycoplasma agalactiae from various specimens of goats. Mehmet Akif Ersoy University Journal of Health Sciences Institute. 2024;12(1):40-5.