Research Article
PDF EndNote BibTex RIS Cite

Buzağılarda Respiratuar Sinsityal Virus (BRSV) Enfeksiyonlarında Patolojik ve İmmunohistokimyasal İncelemeler

Year 2017, Volume 2, Issue 2, 131 - 138, 29.12.2017
https://doi.org/10.24880/maeuvfd.363907

Abstract

Bu çalışmada Burdur İli’ndeki buzağılarda saptanan respiratuar sinsityal virus (BRSV) enfeksiyonları makroskobik, histopatolojik ve immunohistokimyasal bulgularıyla detaylı olarak incelendi. Solunum problemleri ile ölen ve immunohistokimyasal olarak BRSV tespit edilen buzağıların nekropsisinde makroskobik olarak kraniyo-ventral loblarda koyu kırmızı renkli sert kıvamlı pnömoni alanları gözlendi. Bu alanların kesit yüzlerinden köpüklü ve mukopurulent bir eksudat sızıyordu. Mikroskobik olarak akciğer kesitlerinde intersitisyel ve bronko-intersitisyel pnömoni bulguları gözlendi. Pnömoni bölgelerinde yoğun şekilde karakteristik sinsityal hücreler ve bazı bronş-bronşiyol epitellerinde intrasitoplazmik inklüzyon cisimciklerine rastlandı. İmmunohistokimyasal incelemelerde; bronş, bronşiol ve alveol epitelleri ile lümenlerindeki yangısal hücre ve özellikle sinsityal hücrelerin sitoplazmalarında immuno-pozitif alanlara rastlandı. Bu çalışmanın amacı;  Burdur bölgesinde buzağılarda ciddi kayıplara neden olan BRSV hastalığında makroskobik, mikroskobik ve immunohistokimyasal bulgularının detaylı olarak sunulmasıdır.

References

  • 1. Caswett JL Williams KJ. Respiratory system: Infectious disease of the respiratory system, In Jubb, Kennedy, and Palmer’s pathology of domestic animal, Volume 3. 5th edition. Edit. Maxie MG. Elsevier Saunders, Edinburgh; pp: 596-598, 2006.
  • 2. McIntosh KR Chanock M. Respiratory syncytial virus, in: Virology, eds. Fields, B. M. & D. M. Knipe, 2nd edn, Raven Press, New York; pp. 1045-1072, 1990.
  • 3. Ellis JA Philbert H West K Clark E Martin K Haines DM. Fatal pneumonia in adult dairy cattle associated with active infection with bovine respiratory syncytial virus, Can VetJ. 1996; 37: 103-105.
  • 4. Flores EF Weiblen R Medeiros Botton MSA Irigoyen LF Driemeier D Schuch LF Morales MA. Retrospective search for bovine respiratory syncytial virus (BRSV) antigens in histological specimens by immunofluorescence and immunohistochemistry, Pesq Vet Bras. 2000; 20(4): 139-143.
  • 5. Çabalar M Şahna KC. Doğu ve Güneydoğu Anadolu Bölgesinde Süt Sığırlarında Parainfluenza Virus-3, Bovine Herpes Virus-1 ve Respiratory Syncytial Virus Enfeksiyonlarının Seroepidemiyolojisi, Y.Y.Ü. Vet. Fak. Derg. 2000; 11(2): 101-105.
  • 6. Duman R Yavru S Kale M Avci O. Seroprevalence of viral upper respiratory infections in dairy cattle. Kafkas Univ Vet Fak Derg. 2009; 15: 539-542.
  • 7. Yıldırım Y Yılmaz V Majarashin ARF. Kuzeydoğu Anadolu Bölgesi Sınır İllerinde Bulunan Sığırlarda Viral Solunum Sistemi Enfeksiyonlarının Seroprevalansı, Kafkas Univ Vet Fak Derg 2009; 15(4): 601-606.
  • 8. Kale M Ozturk D Hasırcıoglu S Pehlıvanoglu F Turutoglu H. Some Viral and Bacterial Respiratory Tract Infections Of Dairy Cattle During the Summer Season, Acta Veterinaria (Beograd), 2013; 63 (2): 227-236.
  • 9. Hagglund S Svensson C Emanuelson U Valarcher JF Alenius S. Dynamics of virus infections involved in the bovine respiratory disease complex in Swedish dairy herds. Vet J. 2006; 172(2): 320-8.
  • 10. Tripp RA. Pathogenesis of respiratory syncytial virus infection. Viral Immunology, 2004; 17: 165-181.
  • 11. Leung AKC Kellner JD Davies HD. Respiratory syncytial virus bronchiolitis. Journal of the National Medical Association, 2005; 97: 1708-1713.
  • 12. Baker JC Frey ML. Bovine respiratory syncytial virus, Vet. Clin. North Am. Food Anim. Pract. 1985; 1: 259-275.
  • 13. Philippou S Otto P Reinhold P Elschner M Streckert HJ. Respiratory syncytial virus-induced chronic bronchiolitis in experimentally infected calves. Virchows Arch. 2000; 436: 617-621.
  • 14. Viuff B Uttenthal A Tegtmeier C Alexandersen S. Sites of replication of bovine respiratory syncytial virus in naturally infected calves as determined by in situ hybridization. Vet Pathol. 1996; 33: 383-390.
  • 15. Viuff B Tjornehoj K Larsen LE Rontved CM Uttenthal A Ronsholt L Alexandersen S. Replication and clearance of respiratory syncytial virus: apoptosis is an important pathway of virus clearance after experimental infection with bovine respiratory syncytial virus. Am J Pathol. 2002; 161: 2195-2207.
  • 16. Pringle CR. Virus taxonomy – a bulletin from the Xth International Congress of Virology in Jerusalem. Arch Virol. 1996; 141: 2251-2256.
  • 17. Stott E Taylor G. Respiratory syncytial virus: a brief review, Arch. Virol. 1995; 84:1-52.
  • 18. Van der Fels-Klerx HJ Martin SW Nielen M Huirne RBM. Effects on productivity and risk factors of bovine respiratory disease in dairy heiders; a review for the Nehterlands. Neth J Agr Sci 2002; 50: 27-45.
  • 19. Stott E Thomas L Collins A Crouch S Jebbet J Smith G Luther P Caswell R. A survey of virus infections of the respiratory tract of cattle and their association with disease, J. Hyg. (Lond.) 1980; 85: 257-270.
  • 20. Hamvas A. Inherited Surfactant Protein-B Deficiency and Surfactant Protein-C Associated Disease: Clinical Features and Evaluation, Semin Perinatol. 2006; 30(6): 316-26.
  • 21. Jack DL Klein NJ. Mannose-binding lectin: targeting the microbial world for complementMalcolm W. Turner attack and opsonophagocytosis. Immunological Reviews. 2001; 180: 86-99.
  • 22. Laad AD Thomas ML Fakih AR, Chiplunkar SV. Human gamma delta T ce lls recognize heat shock protein-60 on oral tumor cells, Int J Cancer. 1999; 80 (5): 709-714.
  • 23. Xiong H Pamer EG. Monocytes and infection: Modulator, messenger and effector. Immunobiology. 2015; 220: 210-214.

Pathological and Immunohistochemical Studies in Respiratory Cyncitial Virus (BRSV) Infection in Calves

Year 2017, Volume 2, Issue 2, 131 - 138, 29.12.2017
https://doi.org/10.24880/maeuvfd.363907

Abstract

In this study, bovine respiratory syncytial virus (BRSV) infections of calves were examined in detail with macroscopic, microscopic and immunohistochemical findings in Burdur province. At necropsy of calves that died from respiratory findings and immunohistochemically BRSV diagnosed, dark red and hard areas in the cranio-ventral lobes grossly. Foamy and mucopurulent exudate was leaking from the cross-sections of these areas. Microscopically, findings of interstitial and broncho-interstitial pneumonia were observed in the lung sections. At the pneumonic areas, marked characteristic syncytial cells and intra cytoplasmic inclusion bodies were seen in some bronchiolar and bronchial epithelial cells. Immunohistochemically, immuno-positive areas were seen in the bronchial, bronchiolar and alveolar epithelial cells and the cytoplasm of inflammatory cells especially syncytial cells. The aim of this study was detailed presentation of macroscopical, microscopical and immunohistochemical findings of BRSV disease in calves which cause serious economic losses in Burdur province.

References

  • 1. Caswett JL Williams KJ. Respiratory system: Infectious disease of the respiratory system, In Jubb, Kennedy, and Palmer’s pathology of domestic animal, Volume 3. 5th edition. Edit. Maxie MG. Elsevier Saunders, Edinburgh; pp: 596-598, 2006.
  • 2. McIntosh KR Chanock M. Respiratory syncytial virus, in: Virology, eds. Fields, B. M. & D. M. Knipe, 2nd edn, Raven Press, New York; pp. 1045-1072, 1990.
  • 3. Ellis JA Philbert H West K Clark E Martin K Haines DM. Fatal pneumonia in adult dairy cattle associated with active infection with bovine respiratory syncytial virus, Can VetJ. 1996; 37: 103-105.
  • 4. Flores EF Weiblen R Medeiros Botton MSA Irigoyen LF Driemeier D Schuch LF Morales MA. Retrospective search for bovine respiratory syncytial virus (BRSV) antigens in histological specimens by immunofluorescence and immunohistochemistry, Pesq Vet Bras. 2000; 20(4): 139-143.
  • 5. Çabalar M Şahna KC. Doğu ve Güneydoğu Anadolu Bölgesinde Süt Sığırlarında Parainfluenza Virus-3, Bovine Herpes Virus-1 ve Respiratory Syncytial Virus Enfeksiyonlarının Seroepidemiyolojisi, Y.Y.Ü. Vet. Fak. Derg. 2000; 11(2): 101-105.
  • 6. Duman R Yavru S Kale M Avci O. Seroprevalence of viral upper respiratory infections in dairy cattle. Kafkas Univ Vet Fak Derg. 2009; 15: 539-542.
  • 7. Yıldırım Y Yılmaz V Majarashin ARF. Kuzeydoğu Anadolu Bölgesi Sınır İllerinde Bulunan Sığırlarda Viral Solunum Sistemi Enfeksiyonlarının Seroprevalansı, Kafkas Univ Vet Fak Derg 2009; 15(4): 601-606.
  • 8. Kale M Ozturk D Hasırcıoglu S Pehlıvanoglu F Turutoglu H. Some Viral and Bacterial Respiratory Tract Infections Of Dairy Cattle During the Summer Season, Acta Veterinaria (Beograd), 2013; 63 (2): 227-236.
  • 9. Hagglund S Svensson C Emanuelson U Valarcher JF Alenius S. Dynamics of virus infections involved in the bovine respiratory disease complex in Swedish dairy herds. Vet J. 2006; 172(2): 320-8.
  • 10. Tripp RA. Pathogenesis of respiratory syncytial virus infection. Viral Immunology, 2004; 17: 165-181.
  • 11. Leung AKC Kellner JD Davies HD. Respiratory syncytial virus bronchiolitis. Journal of the National Medical Association, 2005; 97: 1708-1713.
  • 12. Baker JC Frey ML. Bovine respiratory syncytial virus, Vet. Clin. North Am. Food Anim. Pract. 1985; 1: 259-275.
  • 13. Philippou S Otto P Reinhold P Elschner M Streckert HJ. Respiratory syncytial virus-induced chronic bronchiolitis in experimentally infected calves. Virchows Arch. 2000; 436: 617-621.
  • 14. Viuff B Uttenthal A Tegtmeier C Alexandersen S. Sites of replication of bovine respiratory syncytial virus in naturally infected calves as determined by in situ hybridization. Vet Pathol. 1996; 33: 383-390.
  • 15. Viuff B Tjornehoj K Larsen LE Rontved CM Uttenthal A Ronsholt L Alexandersen S. Replication and clearance of respiratory syncytial virus: apoptosis is an important pathway of virus clearance after experimental infection with bovine respiratory syncytial virus. Am J Pathol. 2002; 161: 2195-2207.
  • 16. Pringle CR. Virus taxonomy – a bulletin from the Xth International Congress of Virology in Jerusalem. Arch Virol. 1996; 141: 2251-2256.
  • 17. Stott E Taylor G. Respiratory syncytial virus: a brief review, Arch. Virol. 1995; 84:1-52.
  • 18. Van der Fels-Klerx HJ Martin SW Nielen M Huirne RBM. Effects on productivity and risk factors of bovine respiratory disease in dairy heiders; a review for the Nehterlands. Neth J Agr Sci 2002; 50: 27-45.
  • 19. Stott E Thomas L Collins A Crouch S Jebbet J Smith G Luther P Caswell R. A survey of virus infections of the respiratory tract of cattle and their association with disease, J. Hyg. (Lond.) 1980; 85: 257-270.
  • 20. Hamvas A. Inherited Surfactant Protein-B Deficiency and Surfactant Protein-C Associated Disease: Clinical Features and Evaluation, Semin Perinatol. 2006; 30(6): 316-26.
  • 21. Jack DL Klein NJ. Mannose-binding lectin: targeting the microbial world for complementMalcolm W. Turner attack and opsonophagocytosis. Immunological Reviews. 2001; 180: 86-99.
  • 22. Laad AD Thomas ML Fakih AR, Chiplunkar SV. Human gamma delta T ce lls recognize heat shock protein-60 on oral tumor cells, Int J Cancer. 1999; 80 (5): 709-714.
  • 23. Xiong H Pamer EG. Monocytes and infection: Modulator, messenger and effector. Immunobiology. 2015; 220: 210-214.

Details

Primary Language Turkish
Subjects Health Care Sciences and Services
Journal Section Research Articles
Authors

Zafer ÖZYILDIZ> (Primary Author)
Mehmet Akif Ersoy Üniversitesi, Veteriner Fakültesi, Patoloji Anabilim Dalı
0000-0002-6009-9191
Türkiye


Özlem ÖZMEN>
Mehmet Akif Ersoy Üniversitesi, Veteriner Fakültesi, Patoloji Anabilim Dalı
0000-0002-1835-1082
Türkiye


Hüseyin DOLU This is me

Türkiye

Publication Date December 29, 2017
Submission Date December 8, 2017
Acceptance Date December 19, 2017
Published in Issue Year 2017, Volume 2, Issue 2

Cite

APA Özyıldız, Z. , Özmen, Ö. & Dolu, H. (2017). Buzağılarda Respiratuar Sinsityal Virus (BRSV) Enfeksiyonlarında Patolojik ve İmmunohistokimyasal İncelemeler . Veterinary Journal of Mehmet Akif Ersoy University , 2 (2) , 131-138 . DOI: 10.24880/maeuvfd.363907