Comparative Evaluation of Medical versus Surgical Castration on Rat Penile Erectile Tissue Apoptosis

Year 2012, Volume: 4 Issue: 1, 5 - 9, 01.04.2012

Seyit Erkan Eyyüpoğlu Orhun Sinanoğlu Sinan Ekici Memduh Aydın Mete Çek

Abstract

Aim: To evaluate the apoptotic effect of Ciproterone acetate CPA and surgical orchidectomy on rat penile erectile tissue on the basis of Bax protein. Methods: Postpubertal Wistar Albino rats were divided in three groups: First group A underwent bilateral orchidectomy n=10 , second group B received 15 mg intramuscular CPA n=10 every two weeks for 14 days. Third group C; sham operated was taken as control n=10 . All rats were sacrified at 14th day, their cavernous bodies were taken en bloc, stained immunohistochemically with a reagent containing anti-mouse IgG against Bax protein and, cavernous sections were evaluated under microscope with different magnifications. Bax expression differences among groups were analysed with one way ANOVA. Results: No apoptotic difference among proximal, mid and distal penile sections was found. The apoptotic index in surgically castrated group was superior compared to CPA treated group p

Keywords

Apoptosis, penile, cavernous, erectile tissue, surgical castration, ciproterone acetate

Rat Penil Erektil Dokusunda Medikal ve Cerrahi Kastrasyonun Neden Olduğu Apoptozun Karşılaştırmalı Değerlendirilmesi

Abstract

Amaç: Siproteron asetat CPA ve cerrahi orşiektominin rat penil erektil dokusundaki apoptotik etkisini Bax proteinine bağlı olarak değerlendirmek. Yöntem: Postpubertal Wistar Albino ratlar üç gruba ayrıldı. ilk gruba A bilateral orşiektomi n=10 , ikinci gruba B haftada bir 14 gün süreyle 15 mg intramusküler CPA uygulaması n=10 yapıldı. Üçüncü grup C; sham opere kontrol grubu n=10 olarak alındı. Tüm ratlar postoperatif 14. günde sakrifiye edilip penil kavernöz cisimleri blok olarak alındı, Bax proteinine karşı oluşturulmuş anti-mouse IgG içerikli miyarla immünohistokimyasal olarak boyandı ve kavernözal alanlar değişik büyütmelerle mikroskop altında incelendi. Gruplar arasındaki Bax ekspresyonundaki farklı- lıklar one way ANOVA ile değerlendirildi. Bulgular: Proksimal, mid ve distal penil kesitler arasında apoptosis bakımından fark olmadığı görüldü. Cerrahi kastrasyon grubunun penil apoptotik derecesi CPA grubununkinden yüksek bulundu p

Keywords

Apoptoz, penil, kavernöz, erektil doku, cerrahi kastrasyon, siproteron asetat

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