Research Article
Year 2020,
Volume: 12 Issue: 3, 79 - 82, 31.12.2020
Abstract
Amaç: Hücre dışı matriksin en yaygın bileşenlerinden biri olan tip 1 kollajenin, Hepatosellüler Karsinoma (HSK) hücre hattı olan HepG2 hücrelerinde morfoloji ve proliferasyonu üzerine etkilerini incelemek amaçlanmaktadır.
Materyal ve Metodlar: Klasik kültür ve iki boyutlu tip I kollajen kaplı kültür kaplarında HepG2 hücreler 37C° ve %5CO2 varlığında kültüre edildi. Hücreler 72. Saat sonunda %60-80 konfluent oldukları zaman inverted mikroskop altında morfolojik değişimleri görüntülendi ve proliferasyon hızları MTT yöntem ile incelendi.
Bulgular: Tip I kollajen varlığında kültüre edilen hücrelerde morfolojik olarak iğsi bir form gözlenirken, proliferasyon kontrol grubuna göre anlamlı bir şekilde artmıştır.
Sonuç: Tip I kollajen HepG2 hücrelerin, morfolojik özelliklerini değiştirmenin yanında proliferasyon hızlarına da etki göstermektedir.
Keywords
References
- Referans1. Mittal S, and El-Serag BH. Epidemiology of HCC: Consider the Population. J Clin Gastroenterol 2013;47(0):2-6.
- Referans2. Burley MR and Roth CM. Effects of Retinoik Acid on Proliferation and Differention of HepG2 Cells. The Open Biotechnology Journal 2017;1: 47-51.
- Referans3. Moghe PV, Berthiaume F, Ezzell RM, Toner M, Tompkins RG, Yarmush ML. Culture Matrix Configuration and Composition in the Maintanence of Hepatocyte Polarity and Function. Biomaterials 1996; 17: 3.
- Referans4. Dipersio M, Jakson DA, ZaretK S. The Extracellular Matrix Coordinately Modulates Liver Transcription Factors and Hepatocyte Morphology. Molecular and Cellular Biology 1991; 4405-4414.
- Referans5. Kleinman HK, Klebe RJ, Martin GR. Role of Collagenous Matrices in the Adhesion and Growth of Cells. J Cell Bio 1988; 88:473-485.
- Referans6. Zhou M, Zhang Q, Zhao J, Liao M, Wen S and Yang M. Phosphorylation of Bcl-2 plays an important role in glycochenodeoxycholate-induced survival and chemoresistance in HCC. Oncology Reports 2017;38:1742-50.
- Referans7. Deniz G ve Yanıkkaya Demirel G. Akan Hücre Ölçer. 1. Baskı. Yelken Matbaacılık: İstanbul; 2014.
- Referans8. Rieder CL, Cole RW. Cold-shock and the Mammalian cell cycle. Cell cycle 2002;1(3): 169-75.
- Referans9. Roobol A, Carden MJ, Newsam RJ, Smales MJ. Biochemical insights into the mechanisms central to the response of mammalian cells to cold stres and subsequent rewarming. FEBS J 2009;276(1):286-30
- Referans10. Sapudom J and Pompe T. Biomimetic tumor microenvironments based on collagen matrices. Biomater Sci 2018;6:2009-24.
- Referans11. Ehrmann RL and Gey GO. The growth of cells on a transparent gel of reconstituted rat-tail collagen. J Natl Cancer Inst 1956; 16(6): 1375-403.
- Referans12. Godoy P, Hengstler JG, Ilkavets I, Meyer C, Bachmann A, Muller A et al. Extracellular Matrix Modulates Sensitivity of Hepatocytes to Fibroblastoid Dedifferentiation and Transforming Growth Factor β-Induced Apoptosis. Hepatology 2009; 49: 2031-43.
Year 2020,
Volume: 12 Issue: 3, 79 - 82, 31.12.2020
Abstract
References
- Referans1. Mittal S, and El-Serag BH. Epidemiology of HCC: Consider the Population. J Clin Gastroenterol 2013;47(0):2-6.
- Referans2. Burley MR and Roth CM. Effects of Retinoik Acid on Proliferation and Differention of HepG2 Cells. The Open Biotechnology Journal 2017;1: 47-51.
- Referans3. Moghe PV, Berthiaume F, Ezzell RM, Toner M, Tompkins RG, Yarmush ML. Culture Matrix Configuration and Composition in the Maintanence of Hepatocyte Polarity and Function. Biomaterials 1996; 17: 3.
- Referans4. Dipersio M, Jakson DA, ZaretK S. The Extracellular Matrix Coordinately Modulates Liver Transcription Factors and Hepatocyte Morphology. Molecular and Cellular Biology 1991; 4405-4414.
- Referans5. Kleinman HK, Klebe RJ, Martin GR. Role of Collagenous Matrices in the Adhesion and Growth of Cells. J Cell Bio 1988; 88:473-485.
- Referans6. Zhou M, Zhang Q, Zhao J, Liao M, Wen S and Yang M. Phosphorylation of Bcl-2 plays an important role in glycochenodeoxycholate-induced survival and chemoresistance in HCC. Oncology Reports 2017;38:1742-50.
- Referans7. Deniz G ve Yanıkkaya Demirel G. Akan Hücre Ölçer. 1. Baskı. Yelken Matbaacılık: İstanbul; 2014.
- Referans8. Rieder CL, Cole RW. Cold-shock and the Mammalian cell cycle. Cell cycle 2002;1(3): 169-75.
- Referans9. Roobol A, Carden MJ, Newsam RJ, Smales MJ. Biochemical insights into the mechanisms central to the response of mammalian cells to cold stres and subsequent rewarming. FEBS J 2009;276(1):286-30
- Referans10. Sapudom J and Pompe T. Biomimetic tumor microenvironments based on collagen matrices. Biomater Sci 2018;6:2009-24.
- Referans11. Ehrmann RL and Gey GO. The growth of cells on a transparent gel of reconstituted rat-tail collagen. J Natl Cancer Inst 1956; 16(6): 1375-403.
- Referans12. Godoy P, Hengstler JG, Ilkavets I, Meyer C, Bachmann A, Muller A et al. Extracellular Matrix Modulates Sensitivity of Hepatocytes to Fibroblastoid Dedifferentiation and Transforming Growth Factor β-Induced Apoptosis. Hepatology 2009; 49: 2031-43.
There are 12 citations in total.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Clinical Sciences |
| Journal Section | Research Article |
| Authors | |
| Publication Date | December 31, 2020 |
| Submission Date | December 9, 2020 |
| Published in Issue | Year 2020 Volume: 12 Issue: 3 |
