Diagnostic capabilities of kallistatin, IL-10 and IL-1β in patients with non-alcoholic fatty liver disease and hypertension

Year 2021, Volume: 13 Issue: 2, 61 - 69, 31.08.2021

Anastasiia Rozhdestvenska , Natalia Zhelezniakova

https://doi.org/10.35514/mtd.2021.50

Abstract

Introduction. Non-alcoholic fatty liver disease (NAFLD) is closely linked to hypertension (HT) and affects about 25% of the adult population. An important issue remains the search for non-invasive biomarkers for NAFLD diagnosis.
The objective of the study was to evaluate the diagnostic value of kallistatin, interleukin-10 (IL-10) and interleukin-1β (IL-1β) in diagnosis of NAFLD in combination with HT.
Materials and methods. 115 patients with NAFLD at the stage of non-alcoholic steatohepatitis (NASH) were examined, including 63 patients with comorbidity of NASH and HT (main group) and 52 people with isolated NASH (comparison group). Clinical and laboratory parameters were evaluated; plasma kallistatin, IL-10 and IL-1β levels were measured in all patients.
Results. Kallistatin levels averaged 65.03 ng/ml, 83.42 ng/ml and 111.70 ng/ml in patients with NAFLD and HT, isolated NAFLD and control group, respectively. The IL-10 level was 2.69 ng/ml and 4.90 ng/ml in patients with comorbid and isolated NAFLD, respectively, while control results averaged 8.17 ng/ml. The IL-1β level in NAFLD and HT group was 4.76 pg/ml, and in isolated NAFLD group the indicator averaged 4.02 pg/ml, which exceeded the control values (0.59 pg/ml).
Conclusions. The course of both isolated NAFLD and comorbidity NAFLD with HT was accompanied by significant changes of kallistatin, IL-10 and IL-1β levels. Higher HT stage and BP grade, increased BMI and high CRP levels are associated with significantly more pronounced deviations of these indicators in patients with NAFLD and HT. The obtained data provide the possibility to consider the kallistatin, IL-10 and IL-1β as biomarkers of NAFLD severity.

Keywords

kallistatin, IL-10, IL-1β, non-alcoholic fatty liver disease, NAFLD, non-alcoholic steatohepatitis, NASH, hypertension

Thanks

We express our gratitude to the Prof. Dr. Barış ÇAKIR (Maltepe University, Department of Physiology, TURKEY), who offered to publish the article in the “Maltepe Medical Journal” after our presentation in the MEDNS-21 congress.

Alkole bağlı olmayan yağlı karaciğer hastalığı ve hipertansiyonu olan hastalarda kallistatin, IL-10 ve IL1β'nin tanısal güçleri

Abstract

Giriş. Alkolsüz yağlı karaciğer hastalığı, hipertansiyonla yakından bağlantılıdır ve yetişkin nüfusun yaklaşık% 25'ini etkiler. Alkolsüz yağlı karaciğer hastalığı teşhisi için invazif olmayan biyobelirteçlerin araştırılması önemli bir konu olmaya devam etmektedir.
Çalışmanın amacı, hipertansiyon ile kombinasyon halinde alkolsüz yağlı karaciğer hastalığı tanısında kallistatin, interlökin-10 (IL-10) ve interlökin-1β (IL-1β) 'nin tanısal değerini değerlendirmektir.
Malzemeler ve yöntemler. Alkolsüz steatohepatit evresinde alkolsüz yağlı karaciğer hastalığı olan 115 hasta incelendi; bunlara eşlik eden alkolsüz steatohepatit ve hipertansiyonu olan 63 hasta (ana grup) ve izole alkolsüz steatohepatitli 52 kişi (karşılaştırma grubu). Klinik ve laboratuvar parametreleri değerlendirildi; Tüm hastalarda plazma kallistatin, IL-10 ve IL-1β seviyeleri ölçüldü.
Sonuçlar. Alkolsüz yağlı karaciğer hastalığı ve hipertansiyonu olan hastalarda, izole alkolsüz yağlı karaciğer hastalığı ve kontrol grubunda ortalama kallistatin seviyeleri sırasıyla 65.03 ng / ml, 83.42 ng / ml ve 111.70 ng / ml olmuştur. IL-10 seviyesi, komorbid ve izole alkolsüz yağlı karaciğer hastalığı olan hastalarda sırasıyla 2.69 ng / ml ve 4.90 ng / ml iken, kontrol sonuçlarının ortalaması 8.17 ng / ml idi. Alkolsüz yağlı karaciğer hastalığı ve HT grubunda IL-1β seviyesi 4.76 pg / ml idi ve izole alkolsüz yağlı karaciğer hastalığı grubunda gösterge ortalama 4.02 pg / ml idi ve bu da kontrol değerlerini (0.59 pg / ml) aştı. .
Sonuçlar. Hem izole alkolik olmayan yağlı karaciğer hastalığı hem de alkolsüz yağlı karaciğer hastalığı ile birlikte hipertansiyonun seyrine kallistatin, IL-10 ve IL-1p seviyelerinde önemli değişiklikler eşlik etti. Alkolsüz yağlı karaciğer hastalığı ve hipertansiyonu olan hastalarda daha yüksek hipertansiyon evresi ve kan basıncı derecesi, artan vücut kitle indeksi ve yüksek C-reaktif protein seviyeleri, bu göstergelerin önemli ölçüde daha belirgin sapmalarıyla ilişkilidir. Elde edilen veriler, kallistatin, IL-10 ve IL-1β'nın alkolik olmayan yağlı karaciğer hastalığı ciddiyetinin biyolojik belirteçleri olarak dikkate alınmasını sağlar.

Keywords

kallistatin, IL-1β, IL-10, alkolden bağımsız karaciğer yağlanması, yüksek tansiyon

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