Development of Nephelometric Α1-At Method and its Performance Characteristics

Year 2005, Volume: 1 Issue: 6, 29 - 39, 01.01.2005

M. Çalapoğlu O. Değer A. Alver N. Ş. Çalapoğlu E. Kalay C. K. Buruk

https://izlik.org/JA76BD62EH

Abstract

α1-AT, a glycoprotein of molecular mass of 52 kDa, is a major plasma serine protease inhibitor serpin . α1-AT plays a central role as a protease inhibitor in controlling tissue degradation. As a major protease inhibitor in human plasma, α1-AT can complex with a broad spectrum of proteases including elastase, trypsin, chymotrypsin, thrombin, and bacterial leukocyte elastase. α1-AT is an acute phase reactant whose plasma concentration can rise by 3 to 4-fold above normal during inflammation, infection and malignant disease. α1-AT deficiency, one of the most common hereditary disorders, mainly affects the liver and lung [1,2]. The concentrations of α1-AT in plasma can be measured either by functional assays or by immunochemical methods. Functional assays evaluate inhibition of trypsin or elastase. Immunologic methods include radial immunodiffusion, electroimmunoassay and automated nephelometric methods.

References

• D.W. 1-AT deficinecy. In: The Scriver CR, Deaudet AL, Sly WS, Walle D
• α1-AT deficinecy. In: The Scriver CR, Deaudet AL, Sly WS, Walle D
• editors. Metabolic and molecular bases of inherited disease. London: Mc Graw Hill Companies., 1995. p. 4125-4148. 2. MICHAEL, R.N.
• Molecular basis, clinical consequences and diagnosis of α1 AT deficiency. Ann Clin Biochem 1997; 34:230-46.
• CAMPBELL E.J. Alpha1-antitrypsin deficiency: Incidance and detection program. Respiratory Medicine 2000; 94:(Suppl. C):18-21.
• AUSABEL F., BRENT R., KINGSTON R., MOORE D., SEIDMAN J.G., SMİTH J., STRUHL K. Preparation of Polyclonal Antisera. Canada: Greene Puplishing Associates and John Wiley and Sons, 1992. p. 37-41
• PAGE M., THORPE R. Purification of IgG by precipitation with sodium sulfate or ammonium sulfate. In: Walker JM, editor. The Protein Protocols Handbook. Tatowa, New Jersey: Humana Press Inc., 1996. p. 721-2. 6. DEUTSCHER M.P.
• Guide to protein purification. In: Abelson JN, Simon MI
• Editors. Methods in enzymology. California: Academic Press., 1990 p. 57-9, 72-7.
• PAGE M., THORPE R. Purification of using DEAE-sepharose chormatography. In: Walker JM, editor. The protein protocols handbook. Tatowa, New Jersey: Humana Press Inc., 1996. p. 725-6. 8. HAMES B.D.
• One-dimensional polyacryamic gel electrophoresis. In: Hames BD, Rickwood D, editors. Gel electrophoresis of proteins. A practical approach. New York: Oxford University Press., 1990. p.1-59. 9. BAILEY G.S.
• Single radial immunodiffusion. In: Walker JM, editor. The protein protocols handbook. Tatowa, New Jersey: Humana Press Inc., 1996. p.753-5.
• HENRY R.J, CANNON D.C, WINKELMAN J.W, editors. Clinical chemistry: Principles and techniques. New York: Harper & Row., 1974.p. 424-8.
• CARTER J.M. Production and characterization of antibodies aganist peptides. In: Walker JM, editor. The protein protocols handbook. Tatowa, New Jersey: Humana Press Inc., 1996.p. 711-6.
• MICHALSKI J.P., McCOMPS C.C., SHETH S., McCARTHY M., de SAHAZO R.A. Modified double antibady sandwich enzyme-linked ımmunosorbent assay for measurement of α1-AT in biologic fluids. Journal of Immunological Methods 1985; 83: 101-12.
• GEMMELL R.S. Determination of plasma alpha-1-antitrypsin by enzyme- linked immunosorbent assay. Medical Laboratory Sciences 1986; 43:135-9.
• JACKSON G. Immunglobulin quantitation. In: PESCE A.J., KABLAN L.A., editors. Methods in clinical chemistry. USA: The C.V. Mosby Company., 1987 p. 735-41.