New isolates of Streptomyces from Issyk-Kul region soils for biopharmacology.

Abstract

3889 colony forming units of actynomyces have been screened through E.coli cultures containing the resistant to known antibiotics plasmids used in medical practice. 54 isolates of Streptomyces producing potentially new antibiotic compounds have been screened. New PKS genes in soils of Issuk-Kul region have been found as a result of molecular screening by polyketide synthetase primers. The cosmid libraries of PKS genes have been created from soils sampled from Issuk-Kul region

Keywords

• soil,Streptomyces isolates,screening for antibiotic substances,PCR analysis,PKS genes

References

1. Borodina Irina, Scholler С., Eliasson A., Nielsen J. 2005. Metabolic Network Analysis of
2. Streptomyces tenebrarius, a Streptomyces Species with an Active Entner-Doudoroff Pathway. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, May, p. 2294–2302.
3. Гаузе Г.Ф., Преображенская Т.П., Свешникова М.А.. Определитель актиномицетов. Роды Streptomyces, Streptoverticillium, Chainia// М.:Наука, 1983 г.
4. Doolotkeldieva. T. ,Totubaeva.N. 2009. New Strains of Streptomyces as Producers of Biofungicides and Biological Stimulators for Protection of the Shoots and Seedlings of Tiang-Shang Spruce Fir (Picea Schrenkiana)..J.Microbiology Insights:2,1-13, Labertas Academica , New Zealand.
5. Doolotkeldieva, T., Totubaeva.N. Biodiversity of Streptomyces of high-mountainous ecosystems of Kyrgyzstan and its’ biotechnological potential. .// Journal Antonie van Leeuwenhoek ( 2006) 00:1-4.
6. Dunbar et al., AEM No. 4, Vol 65, 1999, pp. 1662-69.
7. Егоров Н.С.1986.Основы учения об антибиотиках. Москва, «Высшая Школа».
8. Kieser, T.,M. J. Bibb,M. J. Buttner, K. F. Chater, and D. A. Hopwood. 2000.

9. General introduction to actinomycete biology, p. 1–42. Practical Streptomyces genetics. The John Innes Foundation, Norwich, United Kingdom. Тотубаева. Н.Э. , Доолоткельдиева Т.Д., . 2004. Биологическая эффективность новых штаммов
10. Streptomyces для стимуляции роста сеянцев хвойных пород. В сбор. «Исследование живой природы БИП НАН КР Stark, W. M., N. G. Knox, and R. M. Wilgus. 1971. Strains of Streptomyces tenebrarius and biosynthesis of nebramycin. Folia Microbiol. (Prague) 16:205–217.
11. Wawrik, B., G. Zylstra, L. Kerkhof and J. Kukor. 2005. Identification of Unique Type II Polyketide Synthase Genes in Soil. Appl. Env. Microbiol. 2005 (5), p. 2232–2238
12. Altschul, S. F., T. L. Madden, A. A. Schaffer, J. Zhang, Z. Zhang, W. Miller, and D. J. Lipman. 1997.
13. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25:3389-3402.
14. Hill TC, Walsh KA, Harris JA, Moffett BF (2003). Using ecological diversity measures with bacterial communities. FEMS Microbiology Ecology 43:1-1