Possibilities of Using Myconanotechnology in Combating Verticillium dahliae Kleb. Fungal Pathogen

Year 2022, , 9 - 14, 30.12.2022

Hilal Acay , Ayfer Yıldırım

https://doi.org/10.30708/mantar.1187449

Abstract

Verticillium dahliae is a soil-borne plant pathogen and its control is limited with chemical fungicides. The disease causing fungus, which causes significant economic agricultural losses all over the world, has a very wide host range. The scarcity of successful methods in the control of the disease further increases the importance of the disease. In addition, the effort to develop alternative control methods is based on the inadequacy of environmentally friendly fungicides, the emergence of fungicide resistance in pathogens and the breaking of host resistance of pathogen populations. Today, the popularity of nanoparticle applications, which provide better efficiency in the control of diseases, is increasing day by day. The current research aims to synthesize a bionanomaterial (RDEK) with the wild mushroom Russula delica Fr. methanol extract (RDE) without using any hazardous substances, with a new, simple, cost-effective and environmentally friendly method, and to point out the use of this synthesized material in agricultural applications as an antifungal agent for the first time. aims.
In the study, it was determined that RDE and RDEK applied in different concentrations showed antifungal activity against Verticillium dahlia. RDEK bionanomaterial, which shows the best activity at 0.02 mg/ml concentration, can be evaluated as a fungicide in agricultural applications.

Keywords

Verticillium dahliae, Fungicide, Russula delica, Bionanomaterial,

Verticillium dahliae Fungal Patojeni İle Mücadelede Mikonanoteknolojinin Kullanım Olanakları

Abstract

Verticillium dahliae toprak kökenli bir bitki patojenidir ve mücadelesi kimyasal fungusitler ile sınırlıdır. Tüm dünyada önemli ekonomik tarımsal kayıplara neden olan hastalık etmeni fungusun çok geniş bir konukçu dizisine sahiptir. Hastalığın kontrolünde başarılı yöntemlerin azlığı, hastalığın önemini daha da arttırmaktadır. Ayrıca alternatif mücadele yöntemleri geliştirilme çabası çevre dostu fungusitlerin yetersizliği, patojenlerde fungisit dayanıklılığının ortaya çıkışı ve patojen popülasyonlarının konukçu dayanıklılığını kırması nedenlerine dayanmaktadır. Günümüzde ise hastalıklarının kontrolünde daha iyi etkinlik sağlayan nanoparçacık uygulamalarının popülerliği her geçen gün artmaktadır. Mevcut araştırma, yabani mantar olan Russula delica metanol ekstresi (RDE) ile herhangi bir tehlikeli madde kullanmadan yeni, basit, uygun maliyetli ve çevre dostu bir yöntem ile biyonanomateryal (RDEK) sentezlemek ve ilk defa sentezlenen bu materyalinantifungal madde olarak tarımsal uygulamlarda kullanımına işaret etmeyi amaçlamaktadır.
Çalışmada, farklı konsnatrasyonlarda uygulanan RDE ve RDEK’nın Verticillium dahlia’ye karşı antifungal aktivite gösterdiği tespit edilmiştir. 0.02 mg/ml konsanrtasyonda en iyi aktivite gösteren RDEK biyonanomateryali tarımsal uygulamlarda fungusit olarak değerlendirilebilir.

Keywords

Verticillium dahlia, Russula delica, Biyonanomateryal, Fungusit, Verticillium dahliae, . Bionanomaterial, Fungicide, Bionanomaterial

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