Nijerya, Lagos Eyaletinden Ganoderma Türlerinin Moleküler Tanımlanması

Abstract

Çok gözenekli bir mantar olan Ganoderma, terapötik özellikleri ve çeşitli sağlık ürünlerinde kullanımı ile yaygın olarak tanınmaktadır. Şu anda Nijerya'nın Lagos kentinden Ganoderma türlerinin moleküler çeşitliliği hakkında herhangi bir bulgu bulunmamaktadır. Bu ön çalışma, Nijerya'nın Lagos Eyaletindeki çeşitli yerlerden toplanan otuz iki yabani Ganoderma türü arasındaki çeşitliliği tespit etmeyi amaçladı. Odun kütüklerinden toplanan otuz iki Ganoderma izolatları, Dahili Transkribe Spacer (ITS1 ve ITS4) bölgesinin genetik analizi ile tanımlandı. Çeşitli dizilerin kaliteli kırpılması ve hizalanması, sırasıyla bioedit yazılımı ve ClustalW algoritması kullanılarak yapıldı. Kalıtım ağacı, MEGA 12 yazılımı ile Komşu Birleştirme yöntemi kullanılarak tahmin edilmiştir. Sonuçlar, toplanan otuz iki örneğin orta, güçlü ve çok güçlü önyükleme değerleri ile desteklenen farklı dalların gösterdiği gibi üç ayrı gruba ayrıldığını ortaya koydu: Ganoderma mbrekobenum (%51-92), G. enigmaticum (%81-99) ve G. amazonense (%91-99) sırasıyla. Bu nedenle, izolatların çoğunluğu Gana'dan gelen izolatlarla en yakın bağlara sahiptir ve bu, Nijerya'dan G. amazonense'nin ilk tanımıdır. Bununla birlikte, izolatların hiçbiri Nijerya'da en çok incelenen tür olan Ganoderma lucidum veya G.applanatum değildi. Diziler referans amacıyla GenBank veri tabanına gönderildi ve filogenetik ağaçta görüldüğü gibi erişim numaraları atandı.

Keywords

• Mantar
• ITS dizisi
• Taksonomi Çeşitlilik
• Filogenetik sistematik
• Batı Afrika

Molecular Identification of Ganoderma Species from Lagos State, Nigeria

Abstract

Ganoderma, a polypore fungus, is widely recognized for its therapeutic properties and use in various health products. There are currently no findings on the molecular diversity of Ganoderma species from Lagos, Nigeria. This preliminary study aimed to ascertain the diversity among thirty-two wild Ganoderma species collected from various locations in Lagos State in Nigeria. Thirty two isolates of Ganoderma collected from wood logs were identified through genetic analysis of Internal Transcribed Spacer (ITS1 and ITS4) region. Quality trimming and alignment of various sequences was done using bioedit software and ClustalW algorithm, respectively. The ancestry tree was estimated using the Neighbor Joining method with MEGA 12 software. The results revealed that the samples collected are separated into three distinct groups as shown by the different clades supported by moderate, strong and very strong bootstrap values, namely Ganoderma mbrekobenum (51-92%) , G. enigmaticum (81-99%), and G. amazonense (91-99%) respectively. Therefore, majority of the isolates have the closest ties to isolates from Ghana and this is the first description of G. amazonense from Nigeria. However, none of the isolates was Ganoderma lucidum or G. applanatum which is the species mostly studied in Nigeria. The sequences were submitted to the GenBank database for reference purposes and were assigned accession numbers as seen on phylogenetic tree.

Keywords

• Mushroom
• ITS sequence
• Taxonomy Diversity
• Phylogenetic systematic
• West Africa

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