Immobilize Pleurotus ostreatus ve Lentinus tigrinus Misellerinin Birlikte Kültürü ile Lakkaz ve Mangan Peroksidaz Enzim Aktivitelerinin Arttırılması

Abstract

Bu çalışmada lignin modifiye edici enzimlerin fazla miktarda üretilmesi için immobilize fungus misellerinin birlikte kültür edilmesi ilk kez olarak rapor edilmiştir. Pleurotus ostreatus OBCC 6043 ve Lentinus tigrinus OBCC 3007 miselleri taşıyıcı olarak kullanılan naylon temizlik süngeri üzerine immobilize edilmiştir. Fungusların immobilize misellerinin ayrı ayrı ve birlikte kültür edilmeleri durumundaki lakkaz ve mangan peroksidaz aktiviteleri karşılaştırılmıştır. Saf Pleurotus ostreatus OBCC 6043 ve Lentinus tigrinus OBCC 3007 kültürlerinin maksimum lakkaz aktiviteleri, sırası ile, 53.73 ve 27.58 U/L iken maksimum mangan peroksidaz aktiviteleri 12.54 ve 52.02 U/L olarak belirlenmiştir. Kokültür koşullarında lakkaz ve mangan peroksidaz aktiviteleri belirgin bir artış ile 319.28 ve 554.33 U/L seviyesine yükselmiştir. Pleurotus ostreatus OBCC 6043 ve Lentinus tigrinus OBCC 3007 birlikte üretildiklerinde lakkaz aktivitesi ayrı ayrı kültür edilmelerine göre, sırası ile,  5.94 ve  11.58 kat fazla bulunmuştur. Diğer taraftan mangan peroksidaz aktivitesi aynı sıra ile  44.21 ve  10.66 kat gibi belirgin biçimde arttırılabilmiştir. Sunulan birlikte kültür çalışmasının sonuçları, hem lakkaz hem de mangan peroksidaz için literatürde sunulan değerlerin çoğuna göre önemli derecede yüksektir.  

Keywords

• İmmobilizasyon,Lakkaz,Lentinus tigrinus,Mangan peroksidaz,Pleurotus ostreatus.

Increasing of Laccase and Manganese Peroxidase Activity by Co-Culture of Immobilized Pleurotus ostreatus and Lentinus tigrinus Mycelia

Abstract

In this study it has firstly reported the using of immobilized fungal mycelia in fungal co-culture studies to enhance activity of lignin-modifying enzymes. For this purpose, the mycelia of Pleurotus ostreatus OBCC 6043 and Lentinus tigrinus OBCC 3007 were immobilized on nylon scouring pad as carrier material. . The immobilized mycelia of the fungi were compared in terms of their laccase and manganese peroxidase activities in mono- and co-culture conditions. The maximum laccase activities of pure Pleurotus ostreatus OBCC 6043 and Lentinus tigrinus OBCC 3007 cultures were determined as 53.73 and 27.58 U/L, respectively, while the maximum manganese peroxidase activities were 12.54 and 52.02 U/L. In co-culture conditions, distinct enhancement was observed in laccase and manganese peroxidase activities as 319.28 and 554.33 U/L, respectively. In the case of laccase, enzyme activity was  5.94 and  11.58 times higher than that of Pleurotus ostreatus OBCC 6043 and Lentinus tigrinus OBCC 3007 mono-cultures, respectively. On the other hand, manganese peroxidase activity could be improved distinctly,  44.21 and  10.66 fold higher values than the corresponding ones. The results of the present co-culture study are significantly higher than most of the reported results in the literature, not only for laccase but also for manganese peroxidase activity.

Keywords

• Immobilization,Laccase,Lentinus tigrinus,Manganese peroxidase,Pleurotus ostreatus

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