CCI4 İle Oksidatif Stres Oluşturulan Sıçanlarda Pholiota aurivella Liyofilize Ekstrenin Bazı Biyokimyasal ve Hematolojik Parametrelere Etkisi

Abstract

Mantarlar, organizmaları bazı zararlı bileşiklerin toksik etkilerinden korumak için önemli işlev görürler. Bu çalışmada, karbon tetraklorür (CCl4) ile oksidatif stres oluşturulan sıçanlarda P.aurivella liyofilize ekstrenin kan parametreleri üzerindeki etkilerinin değerlendirilmesi amaçlanmıştır. Ratlar, toksisite testi sonrası dört deney grubuna ayrıldı; Kontrol, CCI4, CCI4+P.aurivella (100 mg/kg ekstre) ve CCI4+P.aurivella (500 mg/kg ekstre) grupları. 4 haftalık muamele sonunda, CCI4’un oksidatif stresine karşı P.aurivella ekstraktının oral alımının hematolojik parametrelere, eritrosit farjilitesine ve sıçanların eritrositlerinde antioksidan savunma unsurlarından redükte glutatyon (GSH), glutatyon redüktaz (GR), glutatyon S-transferaz (GST), katalaz (CAT), glutatyon peroksidaz (GPx), süperoksid dismutaz (SOD) aktiviteleri ve malondialdehit (MDA) içeriği tespit edilerek değerlendirilmiştir. Sonuçlara göre, CCI4 ve CCI4+P.aurivella ekstresi uygulanan gruplarda GSH düzeyi Kontrol grubuna göre önemli düşüş gösterdi. Diğer yandan, CCI4 grubundaki GPx aktivitesi Kontrol ve CCI4+P.aurivella gruplarına göre önemli düşüş gösterdi. Ayrıca, CCI4 ve CCI4+P.aurivella gruplarında eritrosit frajilitesi (özellikle % 0.6 NaCl konsantrasyonunda) Kontrol grubuna göre önemli artış gözlendi. CCI4 grubunda RBC, HGB, HCT, WBC, PLT and PCT gibi bazı hematolojik parametrelerde önemli azalma gösterirken RDWs de ise önemli artma belirlendi. Bakılan parametreler üzerinde CCI4’ün zararlı etkileri olmasına rağmen bunu P.aurivella için söylemek zordur. Fakat, P.aurivella eritrosit hemolizine katkı sunduğu için zararlı olabilir. Bu yüzden insanların bu mantarı tüketiminde dikkatli davranması gerektiği sonucuna varılmıştır.

Keywords

• CCI4,Pholiota aurivella,antioksidan enzimler,hematolojik parametreler,eritrosit frajilite,sıçan

Effect of Pholiota aurivella Lyophilized Extract On Some Biochemical and Hematological Parameters Against CCI4-İnduced Oxidative Stress In Rats

Abstract

Mushrooms play an important function to protect organisms from the toxic effects of some harmful compounds. In this study, it was aimed to evaluate the effects of Pholiota aurivella lyophilized extract on blood parameters against carbon tetrachloride (CCl4)-induced oxidative stress in rats. After the toxicity test, rats were divided into four experimental groups: Control, CCI4, CCI4+P.aurivella (100 mg/kg, extract) and CCI4+P.aurivella (500 mg/kg, extract) groups. At the end of 4 weeks experiment, the roles of the orally administrated P.aurivella extract oral administration against CCI4-induced oxidative stress were evaluated by measuring hematological parameters, erythrocyte fragility, antioxidant defence system such as reducte glutathione (GSH), glutathione reductase (GR), glutathione S-transferase (GST), catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD) activities and malondialdehyde (MDA) content in erythrocyte of rats. According to the results, CCI4 and CCI4+P.aurivella groups caused significantly decrease in GSH level according to Control group. On the other hand, in CCI4 group GPx enzyme activity significantly decreased according to Control and CCI4+P.aurivella groups. Also, erythrocyte fragility (especially 0.6 % NaCl concentration) significantly increased in the CCI4 and CCI4+P.aurivella groups according to Control group. Significant decrease in some hematological parameters (RBC, HGB, HCT, WBC, PLT and PCT) and markedly increase in RDWs were observed in CCl4 group. Although CCI4 had clear harmful effects on the parameters examined, it was difficult to say this for P.aurivella. However, P.aurivella might be harmful because of its contribution to the hemolysis of erythrocytes. Therefore, people should be careful consumption of this mushroom.

Keywords

• CCI4,Pholiota aurivella,antioxidant enzymes,hematological parameters,erythrocyte fragility,rat

References

1. Acharya K., Chatterjee S., Iswas G., Hatterjee A., Saha G.K., Hepatoprotective effect of a wild edible mushroom on carbon tetrachloride-induced hepatotoxicity in mice. Int. J. Pharm. Pharm. Sci., 4(3): 285-288 (2012).Alkan S., Özparlak, H., Zengin, G., Kaşık G., Antioxidant and In Vitro Some Enzyme Inhibitory Activities of Methanolic Extract of Cultivated Lentinula edodes. Mantar dergisi/The journal of fungus, 8(2): 90-98 (2017).Anand C.V., Anand V., Agarval R., Antioxidant enzymes, gammaglutamyl transpeptidase and lipid peroxidation in kidney of rats exposed to cigarette smoke. Ind. J. Exp. Biol., 34 (5): 486– 488 (1996).Breitenbach J., Kränzlin F., Fungi of Switzerland. Vol.4, Verlag Mykologia Lucerne, Switzerland 1995. p. 368.Chen J.N., Wang Y.T., Wu J.S.B., A glycoprotein extracted from golden oyster mushroom pleurotus citrinopileatus exhibiting growth inhibitory effect against U937 leukemia cells. Journal of Agricultural and Food Chemistry, 57(15): 6706–6711 (2009).Dogan A., Celik I., Kaya M.S., Antidiabetic properties of lyophilized extract of acorn (Quercus brantii Lindl.) on experimentally STZ-induced diabetic rats. Journal of Ethnopharmacology, 176(1): 243–251 (2015).Elshater A.A., Salman M.M., Mohamed S.A.. The hepatoameliorating effect of Solanum nigrum against CCl4 induced liver toxicity in Albino rats. Egypt Acad. J. Biol. Sci., 5 (1): 59–66 (2013).Feeney M.J., Dwyer J., Hasler-Lewis C.M., Milner J., Noakes M., Rowe S., et al., Mushrooms and health summit proceedings. The Journal of Nutrition, 144(1): 1128-1136 (2014).Fujita K., A study on erythrocyte-membrane osmotic resistance and periodontal changes in rats treated with carbon tetrachloride. Shikwa Gakuho, 90(5): 727-743 (1990).Ganie S.A., Haq E., Hamid A., Qurishi Y., Mahmood Z., Zargar B.A., et al., Carbon tetrachloride induced kidney and lung tissue damages and antioxidant activities of the aqueous rhizome extract of Podophyllumhexandrum. BMC Complement Alternative Medicine, 11: 17. doi.org/10.1186/1472-6882-11-17 (2011).Guillamón E., García-Lafuente A., Lozano, M., D’Arrigo M., Rostagno M.A., Villares A., et al., Edible mushroom: Their roles in the prevention of cardiovascular diseases. Fitoterapia, 81(7): 715–723 (2010).Imazeki R., Hongo T., Colored illustrations of mushrooms of Japan, vol I (in Japanese). Hoikusha, Osaka. 1987. p. 207.Jayakumar T., Ramesh E., Geraldine P., Antioxidant activity of the oyster mushroom, Pleurotus ostreatus, on CCl4-induced liver injury in rats. Food and Chemical Toxicology, 44 (12): 1989-1996 (2006).Jin Y., Che T., Yin Y., Yu G., Yang Q., Liu W., et al., Lethal protein in mass consumption edible mushroom Agrocybe aegerita linked to strong hepatic toxicity. Toxicon, 90 (1): 273-285 (2014).Kalaras M.D., Beelman R.B., Holick M.F., Elias R.J., Generation of potentially bioactive ergosterol-derived products following pulsed ultraviolet light exposure of mushrooms (Agaricus bisporus). Food Chemistry, 135(2): 396–401 (2012).Moritz R.P., Pankow D., Effect of carbon tetrachloride and chloroform on hematologic parameters in rats. Folia Haematol., 116 (2): 283–287 (1988).Paraiso L.F., de Freitas M.V., Goncalves E.O.A.F., de Almeida Neto O.P., Pereira E.A., Mascarenhas Netto R.C., et al., Influence of acute exercise on the osmotic stability of the human erythrocyte membrane. Int. J. Sports. Med., 35(13): 1072-7 (2014).Patel S., Goyal A., Recent developments in mushrooms as antiecancer therapeutics: a review. Biotech, 2(1): 1-15 (2012).Rahmouni F., Hamdaoui L., Badraoui R., Rebai T., Protective effects of Teucrium polium aqueous extract and ascorbic acid on hematological and some biochemical parameters against carbon tetrachloride (CCl4) induced toxicity in rats. Biomedicine & Pharmacotherapy, 91(1): 43–48 (2017).Regula J., Siwulski M., Dried Shiitake (Lentunulla edodes) and oyster (Plerotus ostreatus) mushroom as a good source of nutrient. Acta Scientiarum Polonorum Technologia Alimentaria, 6(4): 135–142 (2007).Roupas P., Keogh J., Noakes M., Margetts C., Taylor P., The role of edible mushrooms in health: Evaluation of the evidence. Journal of Functional Foods, 4 (4): 687–709 (2012).Sanchez C., Reactive oxygen species and antioxidant properties from mushrooms. Synthetic and Systems Biotechnology, 1(2):13-22 (2017).Singh A.K., Singh S., Garg G., Rizvi S.I., Rapamycin alleviates oxidative stress-induced damage in rat erythrocytes. Biochemistry and Cell Biology, 94(5): 471-479 (2016).Solak M.H., Gücin F., New records of macrofungi for Turkey from Bursa District and other macrofungi found in the district. Turkish Journal of Botany, 16 (1): 335-346 (1992).Suzek H., Celik I., Dogan A., Yildirim S., Protective effect and antioxidant role of sweetgum (Liquidambar orientalis) oil against carbon tetrachloride-induced hepatotoxicity and oxidative stress in rats. Pharmaceutical Biology, 54 (3): 451–457 (2016).