Some Morphological Properties and Antibacterial Performance of Shiitake (Lentinula edodes) Mushroom Grown in Glass Jar

Abstract

The objective of this study was to determine some morphological characters and antibacterial performance of Shiitake (Lentinula edodes) mushroom grown in the growing medium prepared from a mixture of hornbeam sawdust and wheat bran in the glass jar. In our work, mushroom production was carried out in the growing media prepared in glass jars. Antibacterial activities of the extract prepared in DMSO (10%) from the obtained mushrooms on Enterococcus faecalis (ATCC 29212), Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), Bacillus subtilis (ATCC 6633), Klebsiella pneumoniae and Staphylococcus aureus were investigated using spectrophotometric broth microdilution method. Accordingly, the highest activity was found P. aeruginosa (7.86 mg/ml) while the lowest activity was on B. subtilis (71.28 g/ml). As a result, it was determined that Shiitake mushroom grown in glass jar has morphologically close features to mushroom types grown by other methods and has antimicrobial activity against pathogenic bacteria.

Keywords

• Shiitake (Lentinula edodes),Antibacterial,Microdilution,Hornbeam sawdust

Cam Kavanozda Yetiştirilen Shiitake (Lentinula edodes) Mantarının Bazı Morfolojik Özellikleri ve Antibakteriyel Performansı

Abstract

Bu çalışmanın amacı, cam kavanozda gürgen talaşı ile buğday kepeği karışımından hazırlanan yetiştirme ortamında yetiştirilen Shiitake (Lentinula edodes Berk.) mantarının bazı morfolojik özelliklerini ve antibakteriyel performansını belirlemektir. Çalışmamızda cam kavanozlar içerisinde hazırlanan yetiştirme ortamlarında mantar üretimi gerçekleştirildi. Elde edilen mantarlardan hazırlanan DMSO’ lu (%10) ekstraktın Enterococcus faecalis (ATCC 29212), Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), Bacillus subtilis (ATCC 6633), Klebsiella pneumoniae, Staphylococcus aureus üzerine antibakteriyel aktiviteleri spektrofotometrik broth mikrodilüsyon yöntemi kullanılarak araştırıldı. Buna göre, en düşük aktivite B. subtilis  (71.28 g/ml) bakterisi üzerine iken, en yüksek aktivite P. aeruginosa (7.86 g/ml) üzerine bulundu. Sonuç olarak, cam kavanozda yetiştirilen Shiitake mantarının diğer yöntemlerle üretilen mantar tiplerine morfolojik olarak yakın özellikler taşıdığı ve patojen bakterilere karşı antimikrobiyal  etkinliğe sahip olduğu belirlendi. 

Keywords

• Shiitake (Lentinula edodes),Antibakteriyel,Mikrodilüsyon,Gürgen talaşı

References

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