Preliminary Study on the Determination of Heat Resistant Fungi in Agricultural Soils

Abstract

Heat resistant fungi are capable of surviving temperature at short heat applications. These organisms continue to growing and metabolic activities. Therefore, they cause spoilage of food products and effect on health because of mycotoxin production. This study focused on determination of density and biodiversity of heat resistant fungi in agricultural soil samples. For this aim, two different soil samples were collected from agricultural lands at June 2018. Isolation process was performed by using main soil dilution method after heat treatment at 75°C for 30 minutes of diluted soil samples. After incubation at 25°C for 7-14 days, all colonies were purified. All of the isolates were diagnosed by using conventional methods according to the macromorphological and micromorphological properties. We determined 11 species belong to Alternaria, Aspergillus, Cladosporium, Epicoccum, Humicola, Penicillium and Taloromyces genera. As a result of this study, we showed that agricultural soil areas have a potential for heat resistance fungi biodiversity and exhibited that we should concentrate on this subject with investigation of different areas and sources.

Keywords

• Agricultural Soils,Heat Resistant Fungi,Conventional Method

Tarım Topraklarındaki Isıya Dirençli Fungusların Belirlenmesi Üzerine Ön Çalışma

Abstract

Isıya dayanıklı mantarlar, kısa ısı uygulamalarına dayanabilir. Bu organizmalar büyümeye ve metabolik aktivitelere devam ederler. Bu nedenle, gıda ürünlerinde bozulmaya ve mikotoksin üretme yetenekleri sayesinde sağlık üzerinde etkiye neden olurlar. Bu çalışma, tarımsal toprak örneklerinde ısıya dirençli mantarların yoğunluğu ve biyolojik çeşitliliğinin belirlenmesi üzerine odaklanmıştır. Bu amaçla, Haziran 2018'de tarım alanlarından iki farklı toprak örneği toplanmıştır. İzolasyon işlemi dilüe edilmiş toprak örneklerinin 75 °C’de 30 dakika boyunca ısı uygulaması yapıldıktan sonra toprak dilüsyon yöntemi ile gerçekleştirilmiştir. 25 °C’de 7-14 günlük inkübasyondan sonra tüm koloniler saflaştırılmıştır. İzolatların tamamı makromorfolojik ve mikromorfolojik özelliklere göre geleneksel yöntemler kullanılarak teşhis edilmiştir. Alternaria, Aspergillus, Cladosporium, Epicoccum, Humicola, Penicillium ve Taloromyces cinslerine ait 11 tür tespit edilmiştir. Bu çalışma sonucunda, tarımsal toprak alanlarının ısıya dirençli mantar biyolojik çeşitliliği potansiyeline sahip olduğunu belirledik ve farklı alanların ve kaynakların araştırılmasıyla bu konuya odaklanmamız gerektiğini gösterdik.

Keywords

• Tarım Toprağı,Isıya Dirençli Fungus,Geleneksel Yöntem

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