Armillaria mellea and Macrolepiota procera Ekstrelerinin Antioksidan ve Antimikrobiyal Etkileri

Abstract

Mantarlar, binlerce yıldır besin ve tıbbi değerleri nedeniyle yaygın olarak kullanılmaktadır. Bu çalışma iki yenilebilir mantar olan Armillaria mellea (Vahl) P.Kumm ve Macrolepiota procera (Scop.) antioksidan ve antimikrobiyal potansiyelinin belirlenmesi için tasarlanmıştır Antioksidan aktivitesi DPHH serbest radikal süpürme yöntemi ile tespit edildi. M.procera methanol ekstresi A.mellea methanol ekstresinden daha güçlü serbest radikal süpürücü aktivitesine sahiptir (IC50: 0.191, 1.19 mg/mL). Toplam fenol, β-karoten ve likopen gibi antioksidan özelliklere sahip bileşenlerin konsantrasyonu spektrofotometrik yöntemlerle belirlendi. Son olarak, antimikrobiyal aktivitesi 14 mikroorganizma üzerinde denenmiş olup agar difüzyon yöntemi ile kullanılmıştır. A. mellea metanol ekstraktı Klebsiella pneumaniae ATCC 13883, Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 25923 mikroorganizmlarına karşı 10 ± 1 mm inhibisyon zon çapı oluşturdu. M.procera metanol ekstresi ise Enterococcus faecalis ATCC 29212, Klebsiella pneumaniae ATCC 13883 mikroorganizmlarına karşı 9 ± 1 mm inhibisyon zon çapı oluşturdu Bu araştırma, iki yenilebilir mantarın doğal antioksidanlar olarak potansiyele sahip olduğunu göstermiştir.

Keywords

• Antimikrobiyal aktivite
• mantar
• Macrolepiota procera
• Antioksidan aktivite
• Armillaria mellea

Antioxidant and Antimicrobial Activities of Armillaria mellea and Macrolepiota procera Extracts

Abstract

Mushrooms have been used extensively, owing to their nutritional and medicinal value, for thousands of years. This study designed for the determine of antioxidant and antimicrobial potential of two edible mushrooms Armillaria mellea (Vahl) P.Kumm. and Macrolepiota procera (Scop.) Singer. Antioxidant activity was detected method by DPHH free radical scavenging. M.procera extract had more potent free radical scavenging activity than A.mellea extract (IC50: 0.191, 1.19 mg/mL). The concent of the components with antioxidant properties, such as total phenols,β-caratone and lycopene were determined by spectrophotometric methods. Finally, the antimicrobial potential was determined with a agar well diffusion method on 14 microorganisms. A. mellea methanol extract formed against to Klebsiella pneumaniae ATCC 13883, Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 25923,10±1 mm inhibition zone diameter. M.procera methanol extract formed against to Enterococcus faecalis ATCC 29212, Klebsiella pneumaniae ATCC 13883, 9±1 mm inhibition zone diameter. This research has shown that these two edible mushrooms have potential as natural antioxidants.

Keywords

• Armillea mellea
• Macrolepiota procera
• mushroom
• Antimicrobial activity
• Antioxidant activity

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