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Kültüre Alınmış Lentinula edodes’in Metanol Ekstraktının Antioksidan ve İn Vitro Bazı Enzim İnhibitör Aktiviteleri

Year 2017, Volume 8, Issue 2, 90 - 98, 31.10.2017

Abstract

Shiitake mantarı adıyla da bilinen Lentinula edodes (Berk.) Pegler Doğu Asya’ya özgü yenilebilir bir mantar olup antioksidan ve antimikrobiyal özellikleriyle tedavi edici etkiler içermektedir. Bu çalışmanın ilk kısmı Türkiye’deki kültür L. edodes’in metanolik ekstraktının antioksidan aktivitesini ayrıca total fenolik ve flavonoid içeriğini ilk kez tespit etmeyi amaçladı. Çalışmanın ikinci kısmında, bu mantar ekstraktının kolinesteraz, tirozinaz, amilaz ve glukozidaza karşı enzim inhibitör potansiyelleri araştırıldı. Bu çalışmada rutin çalışmalardan farklı olarak ultrasonik ekstraksiyon yöntemi kullanıldı. Ekstraktaki total fenolik ve flavonoid içerikleri de Folin-Ciocalteu ve AlCl3 yöntemleriyle tespit edildi. Antioksidan aktiviteleri serbest radikal süpürme (DPPH ve ABTS), indirgeme gücü (FRAP ve CUPRAC), fosfomolibdat ve metal şelatlama testleri gibi farklı yöntemlerle araştırıldı. In vitro enzim inhibitör potansiyelleri bir mikroplate okuyucuyla ölçüldü. Bu çalışmada flavonoid içerik ve tirozinaz enzim inhibisyonu tespit edilemedi. Ayrıca L. edodes ekstraktının antioksidan aktivitesi ve enzim inhibisyonu verilerinin özellikle, popüler ve etkili bir başka tıbbi mantar olan Ganoderma lucidum’un ekstraktından elde edilen verilerden daha düşük olduğu dikkat çekti. 

References

  • Afrin S., Rakib M.A., Kim B.H., Kim, J.O., Ha Y.L, Eritadenine from edible mushrooms inhibits activity of angiotensin converting enzyme in vitro, Journal of Agricultural and Food Chemistry, 64: 2263-2268 (2016).
  • Aruoma O.I., Assessment of potential prooxidant and antioxidant actions, Journal of the American Oil Chemists Society, 73(12): 1617-1625 (1996).
  • Carneiro A.A., Ferreira I.C., Dueñas M., Barros L., Da Silva R., Gomes E., Santos-Buelga, C., Chemical composition and antioxidant activity of dried powder formulations of Agaricus blazei and Lentinus edodes, Food Chemistry, 138: 2168-2173 (2013).
  • Chen G.L., Zhang X., Chen S.G., Han M.D., Gao Y.Q., Antioxidant activities and contents of free, esterified and insoluble-bound phenolics in 14 subtropical fruit leaves collected from the south of China, Journal of Functional Foods, 30: 290-302 (2017).
  • Da Silva A.C., Jorge N., Antioxidant properties of Lentinus edodes and Agaricus blazei extracts, Journal of Food Quality, 34: 386-394 (2011).
  • Gheibi N., Zavareh S.H., Behbahani G.R., Haghbeen K., Sirati-sabet M., Ilghari D., Chegini K.G., Comprehensive kinetic and structural studies of different flavonoids inhibiting diphenolase activity of mushroom tyrosinase, Applied Biochemistry and Microbiology, 52: 304-310 (2016).
  • Gil-Ramírez A., Pavo-Caballero C., Baeza E., Baenas N., Garcia-Viguera C., Marín FR., Soler-Rivas C., Mushrooms do not contain flavonoids, Journal of Functional Foods, 25: 1-13. (2016).
  • Hou W.C., Lin R.D., Cheng K.T., Hung Y.T., Cho C.H., Chen C.H., Hwang S.Y., Lee M.H., Free radical scavenging activity of Taiwanese native plants, Phytomedicine, 10: 170-175 (2003).
  • Howes M.J.R., Houghton P.J., Perry N.S.L., Plants with traditional uses and activities, relevant to the management of Alzheimer’s disease and other cognitive disorders, Phytotheraphy Research, 17: 1-18 (2003).
  • Kim Y.J., Uyama H., Tyrosinase inhibitors from natural and synthetic sources: structure, inhibition mechanism and perspective for the future, Cellular and Molecular Life Sciences, 62(15): 1707-23 (2005).
  • Kitzberger C.S.G., Smânia A., Pedrosa R.C., Ferreira S.R.S., Antioxidant and antimicrobial activities of shiitake (Lentinula edodes) extracts obtained by organic solvents and supercritical fluids, Journal of Food Engineering, 80: 631-638 (2007).
  • Laube H., Acarbose: An update of its therapeutic use in diabetes treatment, Clinical Drug Investigation, 22: 141-156 (2002).
  • Melzer D., New drug treatment for alzheimer’s diseases: lessons for healthcare policy, British Medical Journal, 316: 762-764 (1998).
  • Melzer D., New drug treatment for alzheimer’s diseases: lessons for healthcare policy, British Medical Journal, 316: 762-764 (1998).
  • Özçelik E., Pekşen A., Lentinus edodes yetiştiriciliğinde fındık zurufundan hazırlanan farklı yetiştirme ortamlarının verim ve bazı mantar özelliklerine etkileri, 21(1): 65-70 (2006).
  • Özparlak H., Alkan S., Zengin G., Aktümsek A., Türkiye'deki yabani ve kültüre alınmış Ganoderma lucidum’un sulu ekstraktlarının in vitro bazı enzim inhibitör özelliklerinin karşılaştırılması, Mantar Dergisi, 7(2): 110-117 (2016b).
  • Özparlak H., Zengin G., Kaşık G., Türkiye'den yabani ve kültüre alınmış Ganoderma lucidum'un sulu ekstraktlarının antioksidan özelliklerinin karşılaştırılması, Mantar Dergisi, 7(2): 102-109 (2016a).
  • Singh J., Dartois, A., Kaur, L., Starch digestibility in food matrix: A review, Trends in Food Science & Technology, 21: 168-180 (2010).
  • Solano F., Briganti S., Picardo M., Ghanem G., Hypopigmenting agents: an updated review on biological, chemical and clinical aspects, Pigment Cell & Melanoma Research, 19: 550-571 (2006).
  • Tocco G., Fais A., Meli G., Begala M., Podda G., Fadda M B., Corda M., Attanasi O.A., Filippone P., Berretta S., Peg-immobilization of cardol and soluble polymer-supported synthesis of some cardol–coumarin derivatives: preliminary evaluation of their inhibitory activity on mushroom tyrosinase, Bioorganic & Medicinal Chemistry Letters, 19: 36-39 (2009).
  • Tundis R., Loizzo M.R., Menichini F., Natural products as alpha-amylase and alpha-glucosidase inhibitors and their hypoglycaemic potential in the treatment of diabetes: An update, Mini-Reviews in Medicinal Chemistry, 10: 315-331 (2010).
  • Üstün O., Makrofungusların besin değeri ve biyolojik etkileri, Türk Hijyen ve Deneysel Biyoloji Dergisi, 68(4): 223-240 (2011).
  • Zengin G., Sarikurkcu C., Gunes E., Uysal A., Ceylan R., Uysal S., Güngor H., Aktumsek A., Two Ganoderma species: profiling of phenolic compounds by HPLC–DAD, antioxidant, antimicrobial and inhibitory activities on key enzymes linked to diabetes mellitus, Alzheimer's disease and skin disorders, Food Function, 6: 2794-2802 (2015).
  • Zhang XX., Shi QQ., Ji D., Niu LX., Zhang YL., Determination of the phenolic content, profile, and antioxidant activity of seeds from nine tree peony (Paeonia section Moutan DC.) species native to China, Food Research International 97: 141-148 (2017).

Antioxidant and In Vitro Some Enzyme Inhibitory Activities of Methanolic Extract of Cultivated Lentinula edodes

Year 2017, Volume 8, Issue 2, 90 - 98, 31.10.2017

Abstract

Lentinula edodes (Berk.) Pegler, as called Shiitake mushroom, is an edible fungus native to East Asia and contains several therapeutic actions such as antioxidant and antimicrobial properties. The first part of this work aimed to evaluate the antioxidant activity of methanolic extract of cultivated L. edodes in Turkey for the first time, as well as to measure the content of total phenolic and flavonoid compounds of mushroom extract. In the second part of this work, the enzyme inhibitory potentials of this mushroom extract were investigated against cholinesterase, tyrosinase, amylase and glucosidase. In this study, ultrasonication assisted extraction method was used in contrast to routine methods. Total phenolics and flavonoids contents in the extract were determined by Folin-Ciocalteu and AlCl assays. Antioxidant activities were investigated by using different assays, including free radical scavenging assays (DPPH and ABTS) reducing power (FRAP and CUPRAC), phosphomolybdenum and metal chelating assays. The in vitro enzyme inhibitory potentials were measured with a microplate reader. Flavonoid content and tyrosinase enzyme inhibition could not be detected in the present study. It has been noted especially that the antioxidant activity and enzyme inhibition data of the L. edodes extract were lower than those of the Ganoderma lucidum, another popular and effective medical mushroom.

References

  • Afrin S., Rakib M.A., Kim B.H., Kim, J.O., Ha Y.L, Eritadenine from edible mushrooms inhibits activity of angiotensin converting enzyme in vitro, Journal of Agricultural and Food Chemistry, 64: 2263-2268 (2016).
  • Aruoma O.I., Assessment of potential prooxidant and antioxidant actions, Journal of the American Oil Chemists Society, 73(12): 1617-1625 (1996).
  • Carneiro A.A., Ferreira I.C., Dueñas M., Barros L., Da Silva R., Gomes E., Santos-Buelga, C., Chemical composition and antioxidant activity of dried powder formulations of Agaricus blazei and Lentinus edodes, Food Chemistry, 138: 2168-2173 (2013).
  • Chen G.L., Zhang X., Chen S.G., Han M.D., Gao Y.Q., Antioxidant activities and contents of free, esterified and insoluble-bound phenolics in 14 subtropical fruit leaves collected from the south of China, Journal of Functional Foods, 30: 290-302 (2017).
  • Da Silva A.C., Jorge N., Antioxidant properties of Lentinus edodes and Agaricus blazei extracts, Journal of Food Quality, 34: 386-394 (2011).
  • Gheibi N., Zavareh S.H., Behbahani G.R., Haghbeen K., Sirati-sabet M., Ilghari D., Chegini K.G., Comprehensive kinetic and structural studies of different flavonoids inhibiting diphenolase activity of mushroom tyrosinase, Applied Biochemistry and Microbiology, 52: 304-310 (2016).
  • Gil-Ramírez A., Pavo-Caballero C., Baeza E., Baenas N., Garcia-Viguera C., Marín FR., Soler-Rivas C., Mushrooms do not contain flavonoids, Journal of Functional Foods, 25: 1-13. (2016).
  • Hou W.C., Lin R.D., Cheng K.T., Hung Y.T., Cho C.H., Chen C.H., Hwang S.Y., Lee M.H., Free radical scavenging activity of Taiwanese native plants, Phytomedicine, 10: 170-175 (2003).
  • Howes M.J.R., Houghton P.J., Perry N.S.L., Plants with traditional uses and activities, relevant to the management of Alzheimer’s disease and other cognitive disorders, Phytotheraphy Research, 17: 1-18 (2003).
  • Kim Y.J., Uyama H., Tyrosinase inhibitors from natural and synthetic sources: structure, inhibition mechanism and perspective for the future, Cellular and Molecular Life Sciences, 62(15): 1707-23 (2005).
  • Kitzberger C.S.G., Smânia A., Pedrosa R.C., Ferreira S.R.S., Antioxidant and antimicrobial activities of shiitake (Lentinula edodes) extracts obtained by organic solvents and supercritical fluids, Journal of Food Engineering, 80: 631-638 (2007).
  • Laube H., Acarbose: An update of its therapeutic use in diabetes treatment, Clinical Drug Investigation, 22: 141-156 (2002).
  • Melzer D., New drug treatment for alzheimer’s diseases: lessons for healthcare policy, British Medical Journal, 316: 762-764 (1998).
  • Melzer D., New drug treatment for alzheimer’s diseases: lessons for healthcare policy, British Medical Journal, 316: 762-764 (1998).
  • Özçelik E., Pekşen A., Lentinus edodes yetiştiriciliğinde fındık zurufundan hazırlanan farklı yetiştirme ortamlarının verim ve bazı mantar özelliklerine etkileri, 21(1): 65-70 (2006).
  • Özparlak H., Alkan S., Zengin G., Aktümsek A., Türkiye'deki yabani ve kültüre alınmış Ganoderma lucidum’un sulu ekstraktlarının in vitro bazı enzim inhibitör özelliklerinin karşılaştırılması, Mantar Dergisi, 7(2): 110-117 (2016b).
  • Özparlak H., Zengin G., Kaşık G., Türkiye'den yabani ve kültüre alınmış Ganoderma lucidum'un sulu ekstraktlarının antioksidan özelliklerinin karşılaştırılması, Mantar Dergisi, 7(2): 102-109 (2016a).
  • Singh J., Dartois, A., Kaur, L., Starch digestibility in food matrix: A review, Trends in Food Science & Technology, 21: 168-180 (2010).
  • Solano F., Briganti S., Picardo M., Ghanem G., Hypopigmenting agents: an updated review on biological, chemical and clinical aspects, Pigment Cell & Melanoma Research, 19: 550-571 (2006).
  • Tocco G., Fais A., Meli G., Begala M., Podda G., Fadda M B., Corda M., Attanasi O.A., Filippone P., Berretta S., Peg-immobilization of cardol and soluble polymer-supported synthesis of some cardol–coumarin derivatives: preliminary evaluation of their inhibitory activity on mushroom tyrosinase, Bioorganic & Medicinal Chemistry Letters, 19: 36-39 (2009).
  • Tundis R., Loizzo M.R., Menichini F., Natural products as alpha-amylase and alpha-glucosidase inhibitors and their hypoglycaemic potential in the treatment of diabetes: An update, Mini-Reviews in Medicinal Chemistry, 10: 315-331 (2010).
  • Üstün O., Makrofungusların besin değeri ve biyolojik etkileri, Türk Hijyen ve Deneysel Biyoloji Dergisi, 68(4): 223-240 (2011).
  • Zengin G., Sarikurkcu C., Gunes E., Uysal A., Ceylan R., Uysal S., Güngor H., Aktumsek A., Two Ganoderma species: profiling of phenolic compounds by HPLC–DAD, antioxidant, antimicrobial and inhibitory activities on key enzymes linked to diabetes mellitus, Alzheimer's disease and skin disorders, Food Function, 6: 2794-2802 (2015).
  • Zhang XX., Shi QQ., Ji D., Niu LX., Zhang YL., Determination of the phenolic content, profile, and antioxidant activity of seeds from nine tree peony (Paeonia section Moutan DC.) species native to China, Food Research International 97: 141-148 (2017).

Details

Journal Section MYCOLOGY
Authors

Sinan ALKAN
0000-0001-7725-1957
Türkiye


Haluk ÖZPARLAK
SELÇUK ÜNİVERSİTESİ, FEN FAKÜLTESİ, BİYOLOJİ BÖLÜMÜ
Türkiye


Gökhan ZENGİN
Türkiye


Gıyasettin KAŞIK
Türkiye

Publication Date October 31, 2017
Published in Issue Year 2017, Volume 8, Issue 2

Cite

Bibtex @research article { mantar319257, journal = {Mantar Dergisi}, issn = {}, eissn = {2147-6845}, address = {}, publisher = {Selcuk University}, year = {2017}, volume = {8}, pages = {90 - 98}, doi = {}, title = {Kültüre Alınmış Lentinula edodes’in Metanol Ekstraktının Antioksidan ve İn Vitro Bazı Enzim İnhibitör Aktiviteleri}, key = {cite}, author = {Alkan, Sinan and Özparlak, Haluk and Zengin, Gökhan and Kaşık, Gıyasettin} }
APA Alkan, S. , Özparlak, H. , Zengin, G. & Kaşık, G. (2017). Kültüre Alınmış Lentinula edodes’in Metanol Ekstraktının Antioksidan ve İn Vitro Bazı Enzim İnhibitör Aktiviteleri . Mantar Dergisi , 8 (2) , 90-98 . Retrieved from https://dergipark.org.tr/en/pub/mantar/issue/31507/319257
MLA Alkan, S. , Özparlak, H. , Zengin, G. , Kaşık, G. "Kültüre Alınmış Lentinula edodes’in Metanol Ekstraktının Antioksidan ve İn Vitro Bazı Enzim İnhibitör Aktiviteleri" . Mantar Dergisi 8 (2017 ): 90-98 <https://dergipark.org.tr/en/pub/mantar/issue/31507/319257>
Chicago Alkan, S. , Özparlak, H. , Zengin, G. , Kaşık, G. "Kültüre Alınmış Lentinula edodes’in Metanol Ekstraktının Antioksidan ve İn Vitro Bazı Enzim İnhibitör Aktiviteleri". Mantar Dergisi 8 (2017 ): 90-98
RIS TY - JOUR T1 - Kültüre Alınmış Lentinula edodes’in Metanol Ekstraktının Antioksidan ve İn Vitro Bazı Enzim İnhibitör Aktiviteleri AU - Sinan Alkan , Haluk Özparlak , Gökhan Zengin , Gıyasettin Kaşık Y1 - 2017 PY - 2017 N1 - DO - T2 - Mantar Dergisi JF - Journal JO - JOR SP - 90 EP - 98 VL - 8 IS - 2 SN - -2147-6845 M3 - UR - Y2 - 2017 ER -
EndNote %0 The Journal of Fungus Kültüre Alınmış Lentinula edodes’in Metanol Ekstraktının Antioksidan ve İn Vitro Bazı Enzim İnhibitör Aktiviteleri %A Sinan Alkan , Haluk Özparlak , Gökhan Zengin , Gıyasettin Kaşık %T Kültüre Alınmış Lentinula edodes’in Metanol Ekstraktının Antioksidan ve İn Vitro Bazı Enzim İnhibitör Aktiviteleri %D 2017 %J Mantar Dergisi %P -2147-6845 %V 8 %N 2 %R %U
ISNAD Alkan, Sinan , Özparlak, Haluk , Zengin, Gökhan , Kaşık, Gıyasettin . "Kültüre Alınmış Lentinula edodes’in Metanol Ekstraktının Antioksidan ve İn Vitro Bazı Enzim İnhibitör Aktiviteleri". Mantar Dergisi 8 / 2 (October 2017): 90-98 .
AMA Alkan S. , Özparlak H. , Zengin G. , Kaşık G. Kültüre Alınmış Lentinula edodes’in Metanol Ekstraktının Antioksidan ve İn Vitro Bazı Enzim İnhibitör Aktiviteleri. Değerlendirme süreci. 2017; 8(2): 90-98.
Vancouver Alkan S. , Özparlak H. , Zengin G. , Kaşık G. Kültüre Alınmış Lentinula edodes’in Metanol Ekstraktının Antioksidan ve İn Vitro Bazı Enzim İnhibitör Aktiviteleri. Mantar Dergisi. 2017; 8(2): 90-98.
IEEE S. Alkan , H. Özparlak , G. Zengin and G. Kaşık , "Kültüre Alınmış Lentinula edodes’in Metanol Ekstraktının Antioksidan ve İn Vitro Bazı Enzim İnhibitör Aktiviteleri", Mantar Dergisi, vol. 8, no. 2, pp. 90-98, Oct. 2017

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