CCI4 İle Oksidatif Stres Oluşturulan Sıçanlarda Pholiota aurivella Liyofilize Ekstrenin Bazı Biyokimyasal ve Hematolojik Parametrelere Etkisi

Year 2018, Volume: 9 Issue: 1, 58 - 66, 25.04.2018

Abdulahad Doğan

Abstract

Mantarlar, organizmaları bazı zararlı bileşiklerin toksik etkilerinden korumak için önemli işlev görürler. Bu çalışmada, karbon tetraklorür (CCl4) ile oksidatif stres oluşturulan sıçanlarda P.aurivella liyofilize ekstrenin kan parametreleri üzerindeki etkilerinin değerlendirilmesi amaçlanmıştır. Ratlar, toksisite testi sonrası dört deney grubuna ayrıldı; Kontrol, CCI4, CCI4+P.aurivella (100 mg/kg ekstre) ve CCI4+P.aurivella (500 mg/kg ekstre) grupları. 4 haftalık muamele sonunda, CCI4’un oksidatif stresine karşı P.aurivella ekstraktının oral alımının hematolojik parametrelere, eritrosit farjilitesine ve sıçanların eritrositlerinde antioksidan savunma unsurlarından redükte glutatyon (GSH), glutatyon redüktaz (GR), glutatyon S-transferaz (GST), katalaz (CAT), glutatyon peroksidaz (GPx), süperoksid dismutaz (SOD) aktiviteleri ve malondialdehit (MDA) içeriği tespit edilerek değerlendirilmiştir. Sonuçlara göre, CCI4 ve CCI4+P.aurivella ekstresi uygulanan gruplarda GSH düzeyi Kontrol grubuna göre önemli düşüş gösterdi. Diğer yandan, CCI4 grubundaki GPx aktivitesi Kontrol ve CCI4+P.aurivella gruplarına göre önemli düşüş gösterdi. Ayrıca, CCI4 ve CCI4+P.aurivella gruplarında eritrosit frajilitesi (özellikle % 0.6 NaCl konsantrasyonunda) Kontrol grubuna göre önemli artış gözlendi. CCI4 grubunda RBC, HGB, HCT, WBC, PLT and PCT gibi bazı hematolojik parametrelerde önemli azalma gösterirken RDWs de ise önemli artma belirlendi. Bakılan parametreler üzerinde CCI4’ün zararlı etkileri olmasına rağmen bunu P.aurivella için söylemek zordur. Fakat, P.aurivella eritrosit hemolizine katkı sunduğu için zararlı olabilir. Bu yüzden insanların bu mantarı tüketiminde dikkatli davranması gerektiği sonucuna varılmıştır.

Keywords

CCI4, Pholiota aurivella, antioksidan enzimler, hematolojik parametreler, eritrosit frajilite, sıçan

Effect of Pholiota aurivella Lyophilized Extract On Some Biochemical and Hematological Parameters Against CCI4-İnduced Oxidative Stress In Rats

Abstract

Mushrooms play an important function to protect organisms from the toxic effects of some harmful compounds. In this study, it was aimed to evaluate the effects of Pholiota aurivella lyophilized extract on blood parameters against carbon tetrachloride (CCl4)-induced oxidative stress in rats. After the toxicity test, rats were divided into four experimental groups: Control, CCI4, CCI4+P.aurivella (100 mg/kg, extract) and CCI4+P.aurivella (500 mg/kg, extract) groups. At the end of 4 weeks experiment, the roles of the orally administrated P.aurivella extract oral administration against CCI4-induced oxidative stress were evaluated by measuring hematological parameters, erythrocyte fragility, antioxidant defence system such as reducte glutathione (GSH), glutathione reductase (GR), glutathione S-transferase (GST), catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD) activities and malondialdehyde (MDA) content in erythrocyte of rats. According to the results, CCI4 and CCI4+P.aurivella groups caused significantly decrease in GSH level according to Control group. On the other hand, in CCI4 group GPx enzyme activity significantly decreased according to Control and CCI4+P.aurivella groups. Also, erythrocyte fragility (especially 0.6 % NaCl concentration) significantly increased in the CCI4 and CCI4+P.aurivella groups according to Control group. Significant decrease in some hematological parameters (RBC, HGB, HCT, WBC, PLT and PCT) and markedly increase in RDWs were observed in CCl4 group. Although CCI4 had clear harmful effects on the parameters examined, it was difficult to say this for P.aurivella. However, P.aurivella might be harmful because of its contribution to the hemolysis of erythrocytes. Therefore, people should be careful consumption of this mushroom.

Keywords

CCI4, Pholiota aurivella, antioxidant enzymes, hematological parameters, erythrocyte fragility, rat

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