High-Throughput Genomic Simple Sequence Repeat (SSR) Marker Development and Construction of a High Resolution Physical Map in Button Mushroom (Agaricus bisporus) Genome

Year 2018, Volume: 9 Issue: 1, 28 - 35, 25.04.2018

Ali Tevfik Uncu , Ayse Ozgur Uncu

Abstract

Button mushroom [Agaricus bisporus (J.E. Lange) Imbach], is both a model organism and a worldwide cultivated edible mushroom species. Despite its scientific and economic relevance, efforts to develop molecular markers in A. bisporus genome are scarce and relatively recent. The present research reports the development of 3943 novel simple sequence repeat (SSR) markers specific to the A. bisporus genome. The marker set is well-distributed among the 13 A. bisporus chromosomes, saturating the entire genome with sequence-specific markers. The large number of novel, A. bisporus specific DNA markers introduced in the present work constitute a valuable resource for molecular genetic research in button mushroom, including strain identification and protection, and gene/QTL (Quantitative Trait Locus) mapping.

Keywords

Molecular genetics, Bioinformatics, Sequence-specific markers, DNA markers

Kültür Mantarı (Agaricus bisporus) Genomuna Özel Yüksek Miktarda Basit Dizi Tekrarı (SSR) Markörünün Geliştirilmesi ve Yüksek Çözünürlükte Fiziksel Genom Haritasının Oluşturulması

Abstract

Kültür mantarı [Agaricus bisporus (J.E. Lange) Imbach], bir model organizma olmasının yanı sıra dünya genelinde yetiştirilen bir yenilebilir mantar türüdür. Bilimsel ve ekonomik önemine rağmen A. bisporus genomuna özel moleküler markör geliştirme çalışmaları ancak yakın zamanda gerçekleştirilmeye başlanmış olup, oldukça kısıtlı sayıdadır. Bu çalışma kapsamında A. bisporus genomuna özel 3943 adet yeni basit dizi tekrarı (SSR) markörü geliştirilmiştir. Geliştirilen markörler A. bisporus kromozomlarına dengeli şekilde dağılmakta olup, genomun sekans-spesifik markörler ile doyurulmasını sağlamaktadır. Çalışma kapsamında tanıtılmakta olan yüksek sayıdaki A. bisporus’a özel yeni DNA markörü, bu türde gerçekleştirilecek moleküler genetik çalışmalar için kıymetli bir kaynak teşkil edecektir.

Keywords

Moleküler genetik, Biyoinformatik, Sekans-spesifik markörler, DNA markörleri

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