In this
research butter samples were collected from Kastamonu and Erzincan cities
during summer and winter seasons between 2012-2013 years. The aim of this
research is isolate and identify the microfungi in butter samples collected
from various houses in Kastamonu and Erzincan cities. Samples were taken in
sterile conditions, placed in sterile sample containers, hidden at +3 - +6 ° C
temperature with a carrying case, brought to the laboratory and examined.
Microfungi are measured in lacto-phenol preparations after they were reproduced
in artifical nutrient media such as Peptone Dextrose Agar, Malt Extract Agar
(MEA), Potato Dextrose Agar (PDA) and Czapek's Agar (CZ). For the
identifications of the studied species, foreign literature sources are
reviewed. The photographs of isolated species were taken via microscope. During
research, 16 samples were studied, total of 158 fungal colonies and 12 species
belonging to four genera were isolated. The isolated genera in research area
are Aspergillus, Fusarium, Penicillium and Geotrichum. Amongst these, Aspergillus and Penicillium are common. Isolated species in this research are Aspergillus flavus, A.parasiticus, A.versicolor, Fusarium oxysporum, Penicillium chrysogenum,
P.echinulatum, P.griseofulvum, P.palitans, P.roqueforti, P.solitum,
P.verrucosum and Geotrichum candidum. In conclusion, a flora of microfungi has been
found which causes economic and health losses by damaging
the butters in research area.
Bu
araştırmada tereyağı numuneleri 2012-2013 yılları arasında yaz ve kış
mevsimlerinde Kastamonu ve Erzincan
illerinden toplanmıştır. Kastamonu ve Erzincan illerindeki çeşitli evlerden toplanan
tereyağı numunelerindeki mikrofungusların
izolasyonları ve identifikasyonları amaçlanmıştır. Örnekler steril koşullarda
alınmış, steril numune kapları içerisine konulmuş, taşıma çantasıyla +3 - +6 °C’ de saklanmış, laboratuvara getirilmiş ve incelenmiştir. Mikrofunguslar Pepton
Dekstroz Agar, Malt Ekstrakt Agar (MEA), Patates Dekstroz Agar (PDA) ve Czapek’s Agar (CZ) gibi suni besin
ortamlarinda üretildikten sonra, hazırlanan laktofenollü preparatlarında
ölçümleri yapılmıştır. İncelenen türlerin identifikasyonları yabancı eserlere
göre verilmeye çalışılmıştır. İzole edilmiş olan türlerin fotoğrafları
çekilmiştir. Araştırma süresince 16 örnek incelenmiş, toplam 158 fungus
kolonisi ve 4 cinse ait 12 tür izole edilmiştir. Araştırma sahasından izole
edilen cinsler; Aspergillus, Fusarium, Penicillium ve Geotrichum’ dur. Bunlar arasından yaygın
olanlar Aspergillus ve Penicillium’ dur.
Araştırmada izole edilen türler ise Aspergillus
flavus, A.parasiticus, A.versicolor, Fusarium oxysporum, Penicillium
chrysogenum, P.echinulatum, P.griseofulvum, P.palitans, P.roqueforti, P.solitum, P.verrucosum ve Geotrichum
candidum’dur. Sonuç olarak,
araştırma sahasında, tereyağlarında zararlar yaparak, ekonomik ve sağlık
kayıplarına sebep olan bir mikrofungus florası tespit edilmiştir.
Primary Language | Turkish |
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Journal Section | RESEARCH ARTICLE |
Authors | |
Publication Date | October 30, 2019 |
Published in Issue | Year 2019 Volume: 10 Issue: 2 |
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