Biodiversity of Heat Resistance Soil Microfungi in Agricultural Areas of Eskisehir Province

Year 2019, Volume: 10 Issue: 3, 67 - 78, 26.12.2019

Fatma Ayva , Goulsoum Ouzeir , Rasime Demirel , Burhan Şen , Ahmet Asan , Duygu Kadaifçiler

Abstract

Heat-resistant microfungi can survive 30 minutes of heat at 75°C and can
continue to develop and deteriorate products during storage in the room
conditions. The most important role in this heat resistance is based on the
ability to form sexual reproduction structures called ascospores, and
ascospores heat resistance depends on species, strain, pH, heating medium and
other growth. Byssochlamys fulva
(current name; Paecilomyces fulvus)
is the first heat-resistant microfungus recorded, and in addition to B. nivea (current name; Byssochlamys lagunculariae), Neosartorya fischeri (current name; Aspergillus fischeri), Talaromyces macrosporus, T. bacillisporus
and Eupenicillium brefeldianum
(current; Penicillium dodgei) are the
most common heat resistant microfungi. We investigated biodiversity of heat
resistant microfungi in agricultural soils of Eskisehir Province in our study.
For this purpose, four different soil samples were collected from fallow lands
in east, west, north and south locations of Eskisehir Province at September
2017. Isolation process was performed by using heat treatment of soil samples
and the soil dilution method. After purification step, isolates were diagnosed
by using conventional methods and multi locus gene sequencing. We determined
total of 3.22x10³ cfu/g colonies appertain to heat resistant
microfungi and 49 isolates belong to Aspergillus,
Byssochlamys, Penicillium
and Talaromyces genera.
As a result, we determined that the agricultural soils have high heat
resistance microfungal biodiversity that commonly known as mycotoxigenic,
pathogenic and saprophytic.

 

Keywords

Heat resistant microfungi, agricultural soils, Eskisehir, multi locus gene sequencing

Supporting Institution

TUBİTAK, Anadolu University/Eskisehir Technical University Council of Research Project Fund

Project Number

Project Number is 118Z359, Project Number is 1704F102

Thanks

This research was supported by grants from Anadolu University/Eskisehir Technical University Council of Research Project Fund (Project Number is 1704F102) and The Scientific and Technological Research Council of Turkey-TUBITAK (Project Number is 118Z359).

Eskişehir İli Tarım Topraklarındaki Isıya Dirençli Toprak Mikrofunguslarının Biyoçeşitliliği

Abstract

Isıya dayanıklı mikro
mantarlar 75°C'de 30 dakika ısıya dayanabilir ve oda koşullarında depolama
sırasında ürünlerde gelişmeye ve bunlarda bozulmaya devam edebilir. Bu ısı
direncindeki en önemli rol, askospor adı verilen eşeyli üreme yapıları
oluşturma yeteneğine dayanmaktadır ve askosporların ısı direnci; türlere,
strainlere, pH, ısıtma ortamı ve diğer büyüme koşullarına bağlıdır. Byssochlamys fulva (geçerli isim; Paecilomyces fulvus), ilk kaydedilen
ısıya dayanıklı mikrofungusdur ve buna ilave olarak B. nivea (geçerli isim; B.
lagunculariae), Neosartorya fischeri (geçerli isim; Aspergillus fischeri), Talaromyces macrosporus, T. bacillisporus ve Eupenicillium brefeldianum (geçerli
isim; P. dodgei) en yaygın ısıya
dayanıklı mikrofunguslardır. Çalışmamızda, Eskişehir ilinin tarım
topraklarındaki ısıya dayanıklı mikrofungusların biyoçeşitliliği araştırıldı.
Bu amaçla, Eylül 2017'de Eskişehir ilinin doğu, batı, kuzey ve güney
bölgelerindeki nadas alanlarından dört farklı toprak örneği toplanmıştır.
Toprak örneklerinden ısıl işlemi ve toprak seyreltme yöntemi kullanılarak
izolasyon işlemi yapılmıştır. Saflaştırma aşamasından sonra, geleneksel
yöntemler ve çoklu lokus gen dizilimi kullanılarak izolatlar teşhis edilmiştir.
Aspergillus, Byssochlamys, Penicillium ve Talaromyces
cinslerine ait 49 izolat ve ısıya dirençli mikrofunguslara ait toplam 3.22x10³
cfu/g koloni tespit edilmiştir. Sonuç olarak, tarımsal toprakların,
mikotoksijenik, patojenik ve saprofitik olarak bilinen yüksek ısıya dirençli
mikrofungal biyolojik çeşitliliğe sahip olduğunu belirlenmiştir.

Keywords

Isıya dirençli mikrogfunguslar, tarım toprakları, Eskişehir, çoklu gen sekansı

Project Number

Project Number is 118Z359, Project Number is 1704F102

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